MD16G1139400.v1.1

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013145
Physical Location & Seq
Reverse (-)
10737816 .. 10739753
1938 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1139400.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGTGGTGGTCTCAGTCAAAACCACTCATCACCACCGTCAACCTCCTCCTCCTATCCCTCCACCTCCACCTCCTCCCCACCCCCACTCTCTCCCAATGCCGCACCTCCTGCGGCCCCATCCCCATACACTACCCTTTCGCCCTCGATGACGGCTGTGGCGCGCCCCAGTTCCGCCACATGTTCAACTGCTCTACCGACCTCTTCTTCGCCACCCCATCCGGAAACTACAAAGTCCAGTCCATAAACTACGACAAGAAAACCATGGTCGTCTACGACCCCGCCATGTCCACCTGCTCCATCCTCCAGCCCCACCACGACTTCCTCATGACCCCAATCCAGTCCGCCATTATCCCGCCCACCTCCGACACCGTCTTCGCCCTCCTCAACTGCTCCGTCGACTCCCCTGTCCTCAACCACTACAAGTTTCTCTGCTTCAACTTCTCCGGCCACTCCTGTGACGAGCTCTACGACTCCTGCAACGCTTTTCGGATTTTCCGTTCCGGTTCCGGATTGGCTACTCCTACCCCCAGTCCCGGAGGATTTTTAACCAACGGTACTATCCCCAGTCCCGGAGGATTTTTAACCAACGGTACTACCCCCAGTCCCGGAGGATTTCTAACCAACGGTACTCCTCCTCCTGCTGCTGCTACTACGACTACTTCTCCGCCGTGTTGTTTTACGGGGTACGATACGGTGAAGTTCATGAGCATGAATATATTGGACTGCACGCATTATACTAGCGTGACGAATACGGACGGATTGAAAGGGTTAGGACCGCCAGATTGGGTTTACGGGATTAAGCTGAGCTTCGCGGTGCCGGATACCGGGTGTGAGAGGTGTGCGCAGTCCGGTGGGACTTGTGGGTTTGATACCGAGACTGAAGGGTCTCTCTGCCTCTGCTCAACTTCCTCTAATTTCACTAGACAATGTGGTGCAGGCAGTTTTTCAGCGGTAGGACGGGACTGTTCTCCCAATATACCATTCCAAGCATTCTTTGTGATTTTGGGGGCGTTGTTAGTCTTTTTGCATGAGTTTAGGATGCCGAATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

351

Amino Acids

37.66

Weight (kDa)

5.87

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 92 3.9e-12 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 265 - 301 6.6e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17350 AT3G17350
fragaria_vesca FvH4_4g21130
malus_domestica MD16G1139400.v1.1
prunus_persica Prupe.1G108900_v2.0.a1
pyrus_communis pycom16g12380
rosa_chinensis RchiOBHm_Chr4g0426711
rosa_laevigata RLG00000007284
rosa_multiflora Rmu_co8392541.1_g000001 Rmu_sc0010934.1_g000002 Rmu_sc0021908.1_g000002
rosa_roxburghii Rroxscaffold_5G00368680
rosa_rugosa Rorug04G0214400
rosa_samantha Rh4AG269400 Rh4BG275700 Rh4CG290200 Rh4DG272700
rosa_wichuraiana Rw4G023370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 299
AasI GACNNNNNNGTC 2 cut(s) 404, 883
Acc16I TGCGCA 1 cut(s) 843
Acc36I ACCTGC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 814
AccI GTMKAC 2 cut(s) 270, 396
AccII CGCG 2 cut(s) 161, 812
AccIII TCCGGA 2 cut(s) 218, 506
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 1 cut(s) 1045
AcuI CTGAAG 1 cut(s) 900
AfaI GTAC 4 cut(s) 556, 592, 628, 686
AfiI CCNNNNNNNGG 4 cut(s) 533, 569, 605, 824
AflIII ACRYGT 1 cut(s) 177
AgsI TTSAA 3 cut(s) 184, 436, 763
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
AleI CACNNNNGTG 1 cut(s) 453
AloI GAACNNNNNNTCC 2 cut(s) 481, 513
AluBI AGCT 3 cut(s) 463, 802, 807
AluI AGCT 3 cut(s) 463, 802, 807
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 3 cut(s) 15, 869, 891
Aor13HI TCCGGA 2 cut(s) 218, 506
AoxI GGCC 2 cut(s) 112, 445
ApeKI GCWGC 2 cut(s) 641, 644
ApoI RAATTY 1 cut(s) 1045
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 356, 388
AscI GGCGCGCC 1 cut(s) 159
AspLEI GCGC 3 cut(s) 161, 163, 844
AspS9I GGNCC 2 cut(s) 113, 773
AsuC2I CCSGG 4 cut(s) 534, 570, 606, 826
AsuHPI GGTGA 2 cut(s) 22, 706
AvaII GGWCC 1 cut(s) 773
BanI GGYRCC 1 cut(s) 814
BanII GRGCYC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 364
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 2 cut(s) 628, 631
BccI CCATC 3 cut(s) 125, 223, 305
BceAI ACGGC 2 cut(s) 166, 652
BciVI GTATCC 1 cut(s) 814
BcnI CCSGG 4 cut(s) 534, 570, 606, 826
BcoDI GTCTC 3 cut(s) 15, 869, 891
BfaI CTAG 2 cut(s) 738, 921
BfuAI ACCTGC 1 cut(s) 299
BfuI GTATCC 1 cut(s) 814
BisI GCNGC 4 cut(s) 100, 112, 642, 645
BlpI GCTNAGC 1 cut(s) 803
BlsI GCNGC 4 cut(s) 101, 113, 643, 646
Bme1390I CCNGG 4 cut(s) 534, 570, 606, 826
Bme18I GGWCC 1 cut(s) 773
BmgT120I GGNCC 2 cut(s) 113, 773
BmiI GGNNCC 3 cut(s) 115, 505, 816
BmrFI CCNGG 4 cut(s) 534, 570, 606, 826
BmrI ACTGGG 4 cut(s) 160, 522, 558, 594
BmsI GCATC 1 cut(s) 1029
BmuI ACTGGG 4 cut(s) 160, 522, 558, 594
BpiI GAAGAC 1 cut(s) 364
BpmI CTGGAG 1 cut(s) 287
Bpu1102I GCTNAGC 1 cut(s) 803
BpuMI CCSGG 4 cut(s) 534, 570, 606, 826
BsaI GGTCTC 2 cut(s) 15, 891
BsaJI CCNNGG 1 cut(s) 261
BsaWI WCCGGW 4 cut(s) 218, 500, 506, 848
BsaXI ACNNNNNCTCC 4 cut(s) 619, 646, 649, 676
Bsc4I CCNNNNNNNGG 4 cut(s) 533, 569, 605, 824
Bse1I ACTGG 6 cut(s) 166, 235, 337, 528, 564, 600
BseAI TCCGGA 2 cut(s) 218, 506
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 4 cut(s) 117, 215, 297, 1044
BseLI CCNNNNNNNGG 4 cut(s) 533, 569, 605, 824
BseMII CTCAG 2 cut(s) 26, 794
BseNI ACTGG 6 cut(s) 166, 235, 337, 528, 564, 600
BsePI GCGCGC 1 cut(s) 159
BseRI GAGGAG 6 cut(s) 35, 38, 62, 371, 621, 624
BseXI GCAGC 2 cut(s) 628, 631
BsgI GTGCAG 2 cut(s) 709, 954
Bsh1236I CGCG 2 cut(s) 161, 812
BshFI GGCC 2 cut(s) 114, 447
BshNI GGYRCC 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 465
BslFI GGGAC 5 cut(s) 516, 552, 588, 868, 974
BslI CCNNNNNNNGG 4 cut(s) 533, 569, 605, 824
BsmAI GTCTC 3 cut(s) 15, 869, 891
BsmFI GGGAC 5 cut(s) 516, 552, 588, 868, 974
BsmI GAATGC 1 cut(s) 989
BsnI GGCC 2 cut(s) 114, 447
Bso31I GGTCTC 2 cut(s) 15, 891
Bsp1286I GDGCHC 1 cut(s) 465
Bsp13I TCCGGA 2 cut(s) 218, 506
Bsp1720I GCTNAGC 1 cut(s) 803
Bsp19I CCATGG 1 cut(s) 261
BspANI GGCC 2 cut(s) 114, 447
BspCNI CTCAG 2 cut(s) 25, 795
BspEI TCCGGA 2 cut(s) 218, 506
BspFNI CGCG 2 cut(s) 161, 812
BspHI TCATGA 2 cut(s) 324, 702
BspLI GGNNCC 3 cut(s) 115, 505, 816
BspMI ACCTGC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 814
BspTNI GGTCTC 2 cut(s) 15, 891
BsrI ACTGG 6 cut(s) 166, 235, 337, 528, 564, 600
BssECI CCNNGG 1 cut(s) 261
BssHII GCGCGC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 261
Bst4CI ACNGT 7 cut(s) 37, 370, 554, 590, 626, 694, 965
Bst6I CTCTTC 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 108
BstC8I GCNNGC 3 cut(s) 161, 728, 937
BstDEI CTNAG 2 cut(s) 12, 803
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 4 cut(s) 117, 215, 297, 1044
BstFNI CGCG 2 cut(s) 161, 812
BstHHI GCGC 3 cut(s) 161, 163, 844
BstMAI GTCTC 3 cut(s) 15, 869, 891
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 181
BstSCI CCNGG 4 cut(s) 532, 568, 604, 824
BstUI CGCG 2 cut(s) 161, 812
BstV1I GCAGC 2 cut(s) 628, 631
BstV2I GAAGAC 1 cut(s) 364
BsuI GTATCC 1 cut(s) 814
BsuRI GGCC 2 cut(s) 114, 447
BtgI CCRYGG 1 cut(s) 261
BtsCI GGATG 4 cut(s) 117, 215, 297, 1044
BveI ACCTGC 1 cut(s) 299
Cac8I GCNNGC 3 cut(s) 161, 728, 937
CciI TCATGA 2 cut(s) 324, 702
CfoI GCGC 3 cut(s) 161, 163, 844
Cfr13I GGNCC 2 cut(s) 113, 773
Csp6I GTAC 4 cut(s) 555, 591, 627, 685
CviAII CATG 7 cut(s) 178, 262, 283, 325, 703, 709, 1028
CviJI RGCY 8 cut(s) 114, 153, 307, 447, 463, 515, 802, 807
CviKI_1 RGCY 8 cut(s) 114, 153, 307, 447, 463, 515, 802, 807
CviQI GTAC 4 cut(s) 555, 591, 627, 685
DdeI CTNAG 2 cut(s) 12, 803
DrdI GACNNNNNNGTC 2 cut(s) 404, 883
DseDI GACNNNNNNGTC 2 cut(s) 404, 883
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 206
EciI GGCGGA 3 cut(s) 161, 331, 654
Ecl136II GAGCTC 1 cut(s) 463
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 465
Eco31I GGTCTC 2 cut(s) 15, 891
Eco47I GGWCC 1 cut(s) 773
Eco53kI GAGCTC 1 cut(s) 463
Eco57I CTGAAG 1 cut(s) 900
EcoICRI GAGCTC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 7 cut(s) 181, 265, 286, 328, 706, 712, 1031
FalI AAGNNNNNCTT 2 cut(s) 791, 823
FaqI GGGAC 5 cut(s) 516, 552, 588, 868, 974
FatI CATG 7 cut(s) 177, 261, 282, 324, 702, 708, 1027
FauI CCCGC 2 cut(s) 286, 360
FblI GTMKAC 2 cut(s) 270, 396
Fnu4HI GCNGC 4 cut(s) 100, 112, 642, 645
FokI GGATG 3 cut(s) 104, 202, 284
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 4 cut(s) 100, 112, 642, 645
FspBI CTAG 2 cut(s) 738, 921
FspI TGCGCA 1 cut(s) 843
GlaI GCGC 3 cut(s) 160, 162, 843
GluI GCNGC 4 cut(s) 100, 112, 642, 645
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 2 cut(s) 114, 447
HhaI GCGC 3 cut(s) 161, 163, 844
Hin1II CATG 7 cut(s) 181, 265, 286, 328, 706, 712, 1031
Hin6I GCGC 3 cut(s) 159, 161, 842
HinP1I GCGC 3 cut(s) 159, 161, 842
HincII GTYRAC 2 cut(s) 40, 397
HindII GTYRAC 2 cut(s) 40, 397
HinfI GANTC 2 cut(s) 398, 470
HphI GGTGA 2 cut(s) 22, 706
Hpy166II GTNNAC 5 cut(s) 40, 271, 288, 397, 790
Hpy188I TCNGA 2 cut(s) 364, 489
Hpy188III TCNNGA 4 cut(s) 219, 325, 507, 703
Hpy8I GTNNAC 5 cut(s) 40, 271, 288, 397, 790
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 1 cut(s) 875
HpyCH4III ACNGT 7 cut(s) 37, 370, 554, 590, 626, 694, 965
HpyCH4V TGCA 4 cut(s) 477, 726, 935, 1027
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpyF3I CTNAG 2 cut(s) 12, 803
Hsp92II CATG 7 cut(s) 181, 265, 286, 328, 706, 712, 1031
HspAI GCGC 3 cut(s) 159, 161, 842
Kpn2I TCCGGA 2 cut(s) 218, 506
LmnI GCTCC 2 cut(s) 299, 395
Lsp1109I GCAGC 2 cut(s) 628, 631
LweI GCATC 1 cut(s) 1029
MaeI CTAG 2 cut(s) 738, 921
MaeIII GTNAC 2 cut(s) 455, 742
MboII GAAGA 3 cut(s) 193, 196, 364
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 2 cut(s) 913, 1045
MlyI GAGTC 2 cut(s) 392, 464
MmeI TCCRAC 1 cut(s) 387
MroI TCCGGA 2 cut(s) 218, 506
MseI TTAA 3 cut(s) 545, 581, 798
MslI CAYNNNNRTG 2 cut(s) 453, 707
MspA1I CMGCKG 1 cut(s) 950
MspR9I CCNGG 4 cut(s) 534, 570, 606, 826
Mva1269I GAATGC 1 cut(s) 989
MvnI CGCG 2 cut(s) 161, 812
MwoI GCNNNNNNNGC 1 cut(s) 108
NciI CCSGG 4 cut(s) 534, 570, 606, 826
NcoI CCATGG 1 cut(s) 261
NlaIII CATG 7 cut(s) 181, 265, 286, 328, 706, 712, 1031
NlaIV GGNNCC 3 cut(s) 115, 505, 816
NmuCI GTSAC 2 cut(s) 455, 742
NsbI TGCGCA 1 cut(s) 843
NspI RCATGY 1 cut(s) 181
OliI CACNNNNGTG 1 cut(s) 453
PagI TCATGA 2 cut(s) 324, 702
PalAI GGCGCGCC 1 cut(s) 159
PaqCI CACCTGC 1 cut(s) 299
PauI GCGCGC 1 cut(s) 159
PciI ACATGT 1 cut(s) 177
PcsI WCGNNNNNNNCGW 2 cut(s) 465, 493
PctI GAATGC 1 cut(s) 989
PflFI GACNNNGTC 1 cut(s) 368
PfoI TCCNGGA 3 cut(s) 532, 568, 604
PkrI GCNGC 4 cut(s) 101, 113, 643, 646
PleI GAGTC 2 cut(s) 392, 464
PpsI GAGTC 2 cut(s) 392, 464
PscI ACATGT 1 cut(s) 177
Psp124BI GAGCTC 1 cut(s) 465
PspN4I GGNNCC 3 cut(s) 115, 505, 816
PspPI GGNCC 2 cut(s) 113, 773
PsyI GACNNNGTC 1 cut(s) 368
PteI GCGCGC 1 cut(s) 159
RsaI GTAC 4 cut(s) 556, 592, 628, 686
RsaNI GTAC 4 cut(s) 555, 591, 627, 685
RseI CAYNNNNRTG 2 cut(s) 453, 707
SacI GAGCTC 1 cut(s) 465
SalI GTCGAC 1 cut(s) 395
SaqAI TTAA 3 cut(s) 545, 581, 798
SatI GCNGC 4 cut(s) 100, 112, 642, 645
Sau96I GGNCC 2 cut(s) 113, 773
SchI GAGTC 2 cut(s) 392, 464
ScrFI CCNGG 4 cut(s) 534, 570, 606, 826
SduI GDGCHC 1 cut(s) 465
SfaNI GCATC 1 cut(s) 1029
SgsI GGCGCGCC 1 cut(s) 159
SinI GGWCC 1 cut(s) 773
SmiMI CAYNNNNRTG 2 cut(s) 453, 707
Sse9I AATT 2 cut(s) 913, 1045
SspMI CTAG 2 cut(s) 738, 921
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 4 cut(s) 532, 568, 604, 824
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 7 cut(s) 37, 370, 554, 590, 626, 694, 965
TaqI TCGA 2 cut(s) 144, 396
TasI AATT 2 cut(s) 913, 1045
TauI GCSGC 2 cut(s) 102, 114
Tru1I TTAA 3 cut(s) 545, 581, 798
Tru9I TTAA 3 cut(s) 545, 581, 798
TseFI GTSAC 2 cut(s) 455, 742
TseI GCWGC 2 cut(s) 641, 644
Tsp45I GTSAC 2 cut(s) 455, 742
TspDTI ATGAA 2 cut(s) 691, 725
TspGWI ACGGA 4 cut(s) 382, 485, 767, 771
Tth111I GACNNNGTC 1 cut(s) 368
VpaK11BI GGWCC 1 cut(s) 773
XapI RAATTY 1 cut(s) 1045
XceI RCATGY 1 cut(s) 181
XmiI GTMKAC 2 cut(s) 270, 396
XspI CTAG 2 cut(s) 738, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.