Rroxscaffold_5G00368680

Wall-associated receptor kinase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013145
Physical Location & Seq
Forward (+)
50410385 .. 50412169
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00368680.1

Sequence Viewer

Length: 819 bp
ATGAGGTCTCTCTCAAAACCAGTCACCATCATCAACTTCATAGTAGTACTCCTCTCACTCCCTACCCTAACTCTCTCCCAATGCCGCGACTCCTGCGGCGACATCCCCATAAAGTACCCTTTTGCCCTCGATGATGGCTGCGGCGCCGCCCCGCAGTTCCGCCGCATGCTCAACTGCTCCACCGACCTCTTCTTCCTCACCCCGTCCGGCTCCTACAAAGTCGAGTCCATCAACTACGACAAGAAAACCATCGTCGTTTACGACCCCGCCATGTCCACCTGCTCCATCCTCCAGCCCCACCACGACTTCCTCATGACCGATATCCAGTCAGCCCTAATCCCCCCGACCTCCGACACCGTCTTTGCCCTCCTCAACTGCTCTATTGACTCCCCTGTCCTCAACCACTACAGTTACCTCTGCTTCAACTTCTCTTCCCACTCCTGCGACGAGCTCTACGGCTCGTGCAATGCGTTTAGGATTTTCCACATGGTTGCCAACACTACTTCGCCGCCGTGCTGCTTTACAGGTTACGACACCGTTAAGTTCATGAGCATGAACGTCTTGGACTGTACGCATTACACCACGGTGACGAACACGGATGAGTTGAGGGGGGATTGGACCGCAGGATTGGGTTTATGGGATCAAGATGACCTTCGCCGTGCCGGATTCCGGTTGCGAGAGGCCGAATCAAAAGCGGACTTTCACAAAGATGTAAAAGTGCGGACGCGGTTCATAACGGCTACGGGCGGTGTAGATGGTGGCGCTCGTCTTGCGGTGTGCTTAACCGGACCTCCACCTCAAGCTCCTTCTTATCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

272

Amino Acids

30.18

Weight (kDa)

5.94

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 88 6.8e-13 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013145)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17350 AT3G17350
fragaria_vesca FvH4_4g21130
malus_domestica MD16G1139400.v1.1
prunus_persica Prupe.1G108900_v2.0.a1
pyrus_communis pycom16g12380
rosa_chinensis RchiOBHm_Chr4g0426711
rosa_laevigata RLG00000007284
rosa_multiflora Rmu_co8392541.1_g000001 Rmu_sc0010934.1_g000002 Rmu_sc0021908.1_g000002
rosa_roxburghii Rroxscaffold_5G00368680
rosa_rugosa Rorug04G0214400
rosa_samantha Rh4AG269400 Rh4BG275700 Rh4CG290200 Rh4DG272700
rosa_wichuraiana Rw4G023370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 287
AasI GACNNNNNNGTC 1 cut(s) 392
Acc36I ACCTGC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 143
AccII CGCG 2 cut(s) 87, 727
AclWI GGATC 1 cut(s) 648
AcyI GRCGYC 1 cut(s) 144
AfaI GTAC 3 cut(s) 48, 116, 571
AfiI CCNNNNNNNGG 1 cut(s) 669
AgsI TTSAA 1 cut(s) 424
AleI CACNNNNGTG 1 cut(s) 584
AluBI AGCT 2 cut(s) 451, 803
AluI AGCT 2 cut(s) 451, 803
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 1 cut(s) 648
AoxI GGCC 1 cut(s) 681
ApeKI GCWGC 2 cut(s) 138, 516
ArsI GACNNNNNNTTYG 2 cut(s) 344, 376
AspLEI GCGC 2 cut(s) 146, 764
AspS9I GGNCC 2 cut(s) 618, 788
AsuHPI GGTGA 3 cut(s) 16, 190, 598
AvaII GGWCC 2 cut(s) 618, 788
BanI GGYRCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 453
BauI CACGAG 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 125, 503
BccI CCATC 6 cut(s) 35, 128, 236, 257, 293, 749
BceAI ACGGC 4 cut(s) 472, 496, 642, 753
BcoDI GTCTC 1 cut(s) 12
BfmI CTRYAG 1 cut(s) 406
BfoI RGCGCY 2 cut(s) 147, 765
BfuAI ACCTGC 1 cut(s) 287
BisI GCNGC 8 cut(s) 85, 97, 139, 142, 147, 163, 509, 517
BlsI GCNGC 8 cut(s) 86, 98, 140, 143, 148, 164, 510, 518
BmcAI AGTACT 1 cut(s) 48
Bme18I GGWCC 2 cut(s) 618, 788
BmgT120I GGNCC 2 cut(s) 618, 788
BmiI GGNNCC 2 cut(s) 145, 211
BpmI CTGGAG 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 783
BsaHI GRCGYC 1 cut(s) 144
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 582
BsaWI WCCGGW 2 cut(s) 669, 785
BsaXI ACNNNNNCTCC 2 cut(s) 775, 805
Bsc4I CCNNNNNNNGG 1 cut(s) 669
Bse1I ACTGG 2 cut(s) 20, 325
Bse3DI GCAATG 1 cut(s) 472
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 3 cut(s) 102, 285, 604
BseLI CCNNNNNNNGG 1 cut(s) 669
BseMI GCAATG 1 cut(s) 472
BseNI ACTGG 2 cut(s) 20, 325
BseRI GAGGAG 2 cut(s) 41, 359
BseXI GCAGC 2 cut(s) 125, 503
Bsh1236I CGCG 2 cut(s) 87, 727
BshFI GGCC 1 cut(s) 683
BshNI GGYRCC 1 cut(s) 143
BsiHKAI GWGCWC 1 cut(s) 453
BsiSI CCGG 4 cut(s) 207, 663, 670, 786
BslI CCNNNNNNNGG 1 cut(s) 669
BsmAI GTCTC 1 cut(s) 12
BsnI GGCC 1 cut(s) 683
Bso31I GGTCTC 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 453
Bsp143I GATC 1 cut(s) 640
BspANI GGCC 1 cut(s) 683
BspFNI CGCG 2 cut(s) 87, 727
BspHI TCATGA 2 cut(s) 312, 546
BspLI GGNNCC 2 cut(s) 145, 211
BspMI ACCTGC 1 cut(s) 287
BspPI GGATC 1 cut(s) 648
BspT107I GGYRCC 1 cut(s) 143
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 472
BsrI ACTGG 2 cut(s) 20, 325
BssECI CCNNGG 1 cut(s) 582
BssMI GATC 1 cut(s) 640
BssNI GRCGYC 1 cut(s) 144
BssSI CACGAG 1 cut(s) 460
Bst2BI CACGAG 1 cut(s) 460
Bst4CI ACNGT 5 cut(s) 358, 410, 538, 569, 586
Bst6I CTCTTC 2 cut(s) 194, 436
BstACI GRCGYC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 167
BstDSI CCRYGG 1 cut(s) 582
BstF5I GGATG 3 cut(s) 102, 285, 604
BstFNI CGCG 2 cut(s) 87, 727
BstH2I RGCGCY 2 cut(s) 147, 765
BstHHI GCGC 2 cut(s) 146, 764
BstKTI GATC 1 cut(s) 643
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 1 cut(s) 640
BstMWI GCNNNNNNNGC 2 cut(s) 93, 770
BstNSI RCATGY 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 406
BstUI CGCG 2 cut(s) 87, 727
BstV1I GCAGC 2 cut(s) 125, 503
BsuRI GGCC 1 cut(s) 683
BtgI CCRYGG 1 cut(s) 582
BtsCI GGATG 3 cut(s) 102, 285, 604
BveI ACCTGC 1 cut(s) 287
Cac8I GCNNGC 1 cut(s) 167
CciI TCATGA 2 cut(s) 312, 546
CfoI GCGC 2 cut(s) 146, 764
Cfr13I GGNCC 2 cut(s) 618, 788
CseI GACGC 1 cut(s) 733
Csp6I GTAC 3 cut(s) 47, 115, 570
CviAII CATG 6 cut(s) 166, 271, 313, 487, 547, 553
CviJI RGCY 9 cut(s) 138, 210, 295, 332, 451, 459, 683, 740, 803
CviKI_1 RGCY 9 cut(s) 138, 210, 295, 332, 451, 459, 683, 740, 803
CviQI GTAC 3 cut(s) 47, 115, 570
DinI GGCGCC 1 cut(s) 145
DpnI GATC 1 cut(s) 642
DpnII GATC 1 cut(s) 640
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
Eam1104I CTCTTC 2 cut(s) 194, 436
EarI CTCTTC 2 cut(s) 194, 436
EciI GGCGGA 1 cut(s) 149
Ecl136II GAGCTC 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 12
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 618, 788
Eco53kI GAGCTC 1 cut(s) 451
EcoICRI GAGCTC 1 cut(s) 451
EcoRV GATATC 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 453
EgeI GGCGCC 1 cut(s) 145
EheI GGCGCC 1 cut(s) 145
FaeI CATG 6 cut(s) 169, 274, 316, 490, 550, 556
FalI AAGNNNNNCTT 2 cut(s) 636, 668
FatI CATG 6 cut(s) 165, 270, 312, 486, 546, 552
FauI CCCGC 2 cut(s) 159, 274
Fnu4HI GCNGC 8 cut(s) 85, 97, 139, 142, 147, 163, 509, 517
FokI GGATG 3 cut(s) 89, 272, 611
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 8 cut(s) 85, 97, 139, 142, 147, 163, 509, 517
GlaI GCGC 2 cut(s) 145, 763
GluI GCNGC 8 cut(s) 85, 97, 139, 142, 147, 163, 509, 517
GsuI CTGGAG 1 cut(s) 275
HaeII RGCGCY 2 cut(s) 147, 765
HaeIII GGCC 1 cut(s) 683
HapII CCGG 4 cut(s) 207, 663, 670, 786
HgaI GACGC 1 cut(s) 733
HhaI GCGC 2 cut(s) 146, 764
Hin1I GRCGYC 1 cut(s) 144
Hin1II CATG 6 cut(s) 169, 274, 316, 490, 550, 556
Hin6I GCGC 2 cut(s) 144, 762
HinP1I GCGC 2 cut(s) 144, 762
HinfI GANTC 5 cut(s) 89, 224, 386, 666, 686
HpaII CCGG 4 cut(s) 207, 663, 670, 786
HphI GGTGA 3 cut(s) 16, 190, 598
Hpy166II GTNNAC 2 cut(s) 259, 276
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 3 cut(s) 313, 547, 644
Hpy8I GTNNAC 2 cut(s) 259, 276
Hpy99I CGWCG 2 cut(s) 257, 449
HpyAV CCTTC 2 cut(s) 662, 816
HpyCH4III ACNGT 5 cut(s) 358, 410, 538, 569, 586
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 1 cut(s) 465
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 770
HpySE526I ACGT 1 cut(s) 558
Hsp92I GRCGYC 1 cut(s) 144
Hsp92II CATG 6 cut(s) 169, 274, 316, 490, 550, 556
HspAI GCGC 2 cut(s) 144, 762
KasI GGCGCC 1 cut(s) 143
Kzo9I GATC 1 cut(s) 640
LmnI GCTCC 4 cut(s) 182, 215, 287, 808
Lsp1109I GCAGC 2 cut(s) 125, 503
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 4 cut(s) 22, 410, 527, 586
MalI GATC 1 cut(s) 642
MboI GATC 1 cut(s) 640
MboII GAAGA 3 cut(s) 181, 184, 423
MhlI GDGCHC 1 cut(s) 453
Mly113I GGCGCC 1 cut(s) 144
MlyI GAGTC 3 cut(s) 83, 233, 380
MmeI TCCRAC 1 cut(s) 375
MseI TTAA 2 cut(s) 540, 782
MslI CAYNNNNRTG 3 cut(s) 551, 584, 708
MspI CCGG 4 cut(s) 207, 663, 670, 786
MvnI CGCG 2 cut(s) 87, 727
MwoI GCNNNNNNNGC 2 cut(s) 93, 770
NarI GGCGCC 1 cut(s) 144
NdeII GATC 1 cut(s) 640
NlaIII CATG 6 cut(s) 169, 274, 316, 490, 550, 556
NlaIV GGNNCC 2 cut(s) 145, 211
NmuCI GTSAC 2 cut(s) 22, 586
NspI RCATGY 1 cut(s) 169
OliI CACNNNNGTG 1 cut(s) 584
PaeI GCATGC 1 cut(s) 169
PagI TCATGA 2 cut(s) 312, 546
PaqCI CACCTGC 1 cut(s) 287
PcsI WCGNNNNNNNCGW 2 cut(s) 258, 467
PfeI GAWTC 2 cut(s) 666, 686
PflFI GACNNNGTC 1 cut(s) 356
PkrI GCNGC 8 cut(s) 86, 98, 140, 143, 148, 164, 510, 518
PleI GAGTC 3 cut(s) 83, 232, 380
PluTI GGCGCC 1 cut(s) 147
PpsI GAGTC 3 cut(s) 83, 232, 380
Psp124BI GAGCTC 1 cut(s) 453
PspN4I GGNNCC 2 cut(s) 145, 211
PspPI GGNCC 2 cut(s) 618, 788
PsyI GACNNNGTC 1 cut(s) 356
RsaI GTAC 3 cut(s) 48, 116, 571
RsaNI GTAC 3 cut(s) 47, 115, 570
RseI CAYNNNNRTG 3 cut(s) 551, 584, 708
SacI GAGCTC 1 cut(s) 453
SaqAI TTAA 2 cut(s) 540, 782
SatI GCNGC 8 cut(s) 85, 97, 139, 142, 147, 163, 509, 517
Sau3AI GATC 1 cut(s) 640
Sau96I GGNCC 2 cut(s) 618, 788
ScaI AGTACT 1 cut(s) 48
SchI GAGTC 3 cut(s) 83, 233, 380
SduI GDGCHC 1 cut(s) 453
SfcI CTRYAG 1 cut(s) 406
SfoI GGCGCC 1 cut(s) 145
SinI GGWCC 2 cut(s) 618, 788
SmiMI CAYNNNNRTG 3 cut(s) 551, 584, 708
SmlI CTYRAG 1 cut(s) 798
SmoI CTYRAG 1 cut(s) 798
SphI GCATGC 1 cut(s) 169
SspDI GGCGCC 1 cut(s) 143
SstI GAGCTC 1 cut(s) 453
TaaI ACNGT 5 cut(s) 358, 410, 538, 569, 586
TaiI ACGT 1 cut(s) 561
TaqI TCGA 2 cut(s) 129, 222
TaqII GACCGA 1 cut(s) 332
TatI WGTACW 1 cut(s) 46
TauI GCSGC 6 cut(s) 87, 99, 144, 149, 165, 511
TfiI GAWTC 2 cut(s) 666, 686
Tru1I TTAA 2 cut(s) 540, 782
Tru9I TTAA 2 cut(s) 540, 782
TseFI GTSAC 2 cut(s) 22, 586
TseI GCWGC 2 cut(s) 138, 516
Tsp45I GTSAC 2 cut(s) 22, 586
TspDTI ATGAA 4 cut(s) 28, 535, 569, 721
TspGWI ACGGA 1 cut(s) 611
Tth111I GACNNNGTC 1 cut(s) 356
VpaK11BI GGWCC 2 cut(s) 618, 788
XceI RCATGY 1 cut(s) 169
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.