FvH4_4g21870

50S ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009178
Physical Location & Seq
Forward (+)
24851796 .. 24853768
1973 bp
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UTR
Exon/CDS
Intron
FvH4_4g21870.t1

Sequence Viewer

Length: 480 bp
ATGTCAGCGTCTCTTTCGTTTGTTGGGTCTTCCCAACAGCTTTGGATGCGTCCTAAGTTTGTAGGAGTATCTGCTCCAAAGCCTCAATCTCAGTCTCTAGTTATCGAAGCCAAGGCCACTACCAAAAGAGAAGACAGGACCGCTCGCCATATTCGTATCAGAAAGAAGGTTGAAGGAACTACCGAAAGGCCAAGACTATGTGTCTTCCGCTCCAACAAGCATCTCTATGTCCAAGTGATTGATGACACCAAGATGCATACACTTGCTTCAGCTTCAACAATGCAGAAGCCCATCTCTGAGGAGTTTGACTACACTTCTGGTCCTACCATTGAAGTAGCAAAGAAGGTGGGAGAAGTCATTGCAAAGTCTTGTTTGGAGAAAGGGATCACGAAGGTAGCCTTTGACCGAGGTGGCTATCCGTATCATGGACGTGTGGAAGCCCTTGCTAATGCAGCTAGGGAGAACGGTCTTCAATTCTAA

Protein Analysis

160

Amino Acids

17.71

Weight (kDa)

9.82

Isoelectric Point (pI)

52.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 41 - 159 8.3e-47 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 143, 210
AciI CCGC 2 cut(s) 141, 208
AclWI GGATC 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 252
AfiI CCNNNNNNNGG 1 cut(s) 425
AflIII ACRYGT 1 cut(s) 430
AgsI TTSAA 4 cut(s) 173, 276, 332, 473
AhdI GACNNNNNGTC 1 cut(s) 200
AjiI CACGTC 1 cut(s) 431
AluBI AGCT 3 cut(s) 40, 272, 455
AluI AGCT 3 cut(s) 40, 272, 455
Alw26I GTCTC 2 cut(s) 15, 99
AlwI GGATC 1 cut(s) 392
AoxI GGCC 2 cut(s) 114, 188
ApeKI GCWGC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 138, 320
AvaII GGWCC 2 cut(s) 138, 320
BbsI GAAGAC 4 cut(s) 21, 138, 196, 461
BbvI GCAGC 1 cut(s) 464
BccI CCATC 1 cut(s) 299
BcoDI GTCTC 2 cut(s) 15, 99
BfaI CTAG 2 cut(s) 98, 456
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme18I GGWCC 2 cut(s) 138, 320
BmeRI GACNNNNNGTC 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 138, 320
BmsI GCATC 3 cut(s) 36, 229, 243
BpiI GAAGAC 4 cut(s) 21, 138, 196, 461
BsaJI CCNNGG 2 cut(s) 111, 406
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 357
BseDI CCNNGG 2 cut(s) 111, 406
BseGI GGATG 1 cut(s) 51
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 2 cut(s) 104, 288
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 1 cut(s) 464
BshFI GGCC 2 cut(s) 116, 190
BslI CCNNNNNNNGG 1 cut(s) 425
BsmAI GTCTC 2 cut(s) 15, 99
BsmBI CGTCTC 1 cut(s) 15
BsnI GGCC 2 cut(s) 116, 190
Bsp143I GATC 1 cut(s) 384
BspACI CCGC 2 cut(s) 141, 208
BspANI GGCC 2 cut(s) 116, 190
BspCNI CTCAG 2 cut(s) 103, 289
BspPI GGATC 1 cut(s) 392
BsrBI CCGCTC 2 cut(s) 143, 210
BsrDI GCAATG 1 cut(s) 357
BssECI CCNNGG 2 cut(s) 111, 406
BssMI GATC 1 cut(s) 384
BssT1I CCWWGG 1 cut(s) 111
Bst4CI ACNGT 1 cut(s) 467
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 3 cut(s) 54, 90, 297
BstF5I GGATG 1 cut(s) 51
BstKTI GATC 1 cut(s) 387
BstMAI GTCTC 2 cut(s) 15, 99
BstMBI GATC 1 cut(s) 384
BstMWI GCNNNNNNNGC 2 cut(s) 46, 452
BstV1I GCAGC 1 cut(s) 464
BstV2I GAAGAC 4 cut(s) 21, 138, 196, 461
BsuRI GGCC 2 cut(s) 116, 190
BtrI CACGTC 1 cut(s) 431
BtsCI GGATG 1 cut(s) 51
Cac8I GCNNGC 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 138, 320
CseI GACGC 1 cut(s) 38
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 1 cut(s) 425
DdeI CTNAG 3 cut(s) 54, 90, 297
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
DriI GACNNNNNGTC 1 cut(s) 200
Eam1105I GACNNNNNGTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 111
Eco47I GGWCC 2 cut(s) 138, 320
Eco57I CTGAAG 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 1 cut(s) 428
FaiI YATR 5 cut(s) 150, 199, 228, 258, 426
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FatI CATG 1 cut(s) 424
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 1 cut(s) 58
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 2 cut(s) 98, 456
GluI GCNGC 1 cut(s) 453
HaeIII GGCC 2 cut(s) 116, 190
HgaI GACGC 1 cut(s) 38
Hin1II CATG 1 cut(s) 428
Hpy188I TCNGA 2 cut(s) 161, 298
Hpy188III TCNNGA 1 cut(s) 388
HpyAV CCTTC 4 cut(s) 160, 167, 337, 385
HpyCH4III ACNGT 1 cut(s) 467
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 4 cut(s) 256, 283, 362, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 452
HpyF3I CTNAG 3 cut(s) 54, 90, 297
HpySE526I ACGT 1 cut(s) 430
Hsp92II CATG 1 cut(s) 428
Kzo9I GATC 1 cut(s) 384
LmnI GCTCC 2 cut(s) 79, 215
LpnPI CCDG 2 cut(s) 121, 303
Lsp1109I GCAGC 1 cut(s) 464
LweI GCATC 3 cut(s) 36, 229, 243
MaeI CTAG 2 cut(s) 98, 456
MaeII ACGT 1 cut(s) 430
MalI GATC 1 cut(s) 386
MbiI CCGCTC 2 cut(s) 143, 210
MboI GATC 1 cut(s) 384
MboII GAAGA 4 cut(s) 21, 143, 196, 461
MluCI AATT 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 237
MnlI CCTC 3 cut(s) 93, 292, 401
Mph1103I ATGCAT 1 cut(s) 258
MslI CAYNNNNRTG 3 cut(s) 225, 251, 429
MwoI GCNNNNNNNGC 2 cut(s) 46, 452
NdeII GATC 1 cut(s) 384
NlaIII CATG 1 cut(s) 428
NsiI ATGCAT 1 cut(s) 258
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PkrI GCNGC 1 cut(s) 454
PspPI GGNCC 2 cut(s) 138, 320
RseI CAYNNNNRTG 3 cut(s) 225, 251, 429
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 2 cut(s) 138, 320
SetI ASST 8 cut(s) 42, 171, 274, 348, 396, 412, 433, 457
SfaNI GCATC 3 cut(s) 36, 229, 243
SinI GGWCC 2 cut(s) 138, 320
SmiMI CAYNNNNRTG 3 cut(s) 225, 251, 429
Sse9I AATT 1 cut(s) 473
SsiI CCGC 2 cut(s) 141, 208
SspMI CTAG 2 cut(s) 98, 456
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 467
TaiI ACGT 1 cut(s) 433
TaqI TCGA 1 cut(s) 105
TaqII GACCGA 1 cut(s) 420
TasI AATT 1 cut(s) 473
TseI GCWGC 1 cut(s) 452
TspGWI ACGGA 1 cut(s) 408
VpaK11BI GGWCC 2 cut(s) 138, 320
XspI CTAG 2 cut(s) 98, 456
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.