Rh4CG300800

50S ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0009178
Physical Location & Seq
Forward (+)
56120514 .. 56122240
1727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG300800.1

Sequence Viewer

Length: 336 bp
ATGGCTCATTTCTTGGTTTTTCAGGTTGAAGGAACTACCGAAAGGCCAAGACTATGTGTCTTCCGCTCCAACAAGCATCTCTATGTCCAAGTGATTGATGATACCAAGATGCATACACTTGCTTCAGCTTCAACAATGCAGAAGCCCATCTCTGAGGAGTTTGACTTCACTTCTGGTCCAACCATTGAAGTAGCAAAGAAGGTGGGAGAAGTCATTGCAAAGTCATGTTTGGAGAAAGGGATCACAAAGGTAGCCTTCGACCGAGGTGGCTATCCGTATCATGGACGTGTGGAAGCCCTTGCTACTGCTGCTAGGGAGAATGGCCTTCAATTCTGA

Protein Analysis

111

Amino Acids

12.35

Weight (kDa)

7.82

Isoelectric Point (pI)

31.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 8 - 111 4.7e-40 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 66
AciI CCGC 1 cut(s) 64
AclWI GGATC 1 cut(s) 248
AcuI CTGAAG 1 cut(s) 108
AfiI CCNNNNNNNGG 1 cut(s) 281
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 4 cut(s) 29, 132, 188, 329
AhdI GACNNNNNGTC 1 cut(s) 56
AjiI CACGTC 1 cut(s) 287
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
AlwI GGATC 1 cut(s) 248
AoxI GGCC 2 cut(s) 44, 322
ApeKI GCWGC 1 cut(s) 308
AspS9I GGNCC 1 cut(s) 176
AvaII GGWCC 1 cut(s) 176
BbsI GAAGAC 1 cut(s) 52
BbvI GCAGC 1 cut(s) 295
BccI CCATC 1 cut(s) 155
BfaI CTAG 1 cut(s) 312
BisI GCNGC 1 cut(s) 309
BlsI GCNGC 1 cut(s) 310
Bme18I GGWCC 1 cut(s) 176
BmeRI GACNNNNNGTC 1 cut(s) 56
BmgBI CACGTC 1 cut(s) 287
BmgT120I GGNCC 1 cut(s) 176
BmsI GCATC 2 cut(s) 85, 99
BpiI GAAGAC 1 cut(s) 52
BsaJI CCNNGG 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 281
Bse3DI GCAATG 1 cut(s) 213
BseDI CCNNGG 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 144
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 1 cut(s) 295
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 2 cut(s) 46, 324
BsiEI CGRYCG 1 cut(s) 262
BslI CCNNNNNNNGG 1 cut(s) 281
BsnI GGCC 2 cut(s) 46, 324
Bsp143I GATC 1 cut(s) 240
BspACI CCGC 1 cut(s) 64
BspANI GGCC 2 cut(s) 46, 324
BspCNI CTCAG 1 cut(s) 145
BspPI GGATC 1 cut(s) 248
BsrBI CCGCTC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 213
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 153
BstKTI GATC 1 cut(s) 243
BstMBI GATC 1 cut(s) 240
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstV1I GCAGC 1 cut(s) 295
BstV2I GAAGAC 1 cut(s) 52
BsuRI GGCC 2 cut(s) 46, 324
BtrI CACGTC 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 176
CspCI CAANNNNNGTGG 2 cut(s) 183, 218
CviAII CATG 2 cut(s) 225, 281
CviJI RGCY 8 cut(s) 5, 46, 128, 145, 254, 270, 296, 324
CviKI_1 RGCY 8 cut(s) 5, 46, 128, 145, 254, 270, 296, 324
DdeI CTNAG 1 cut(s) 153
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
DriI GACNNNNNGTC 1 cut(s) 56
Eam1105I GACNNNNNGTC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 108
EcoT22I ATGCAT 1 cut(s) 114
FaeI CATG 2 cut(s) 228, 284
FaiI YATR 5 cut(s) 55, 84, 114, 226, 282
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FatI CATG 2 cut(s) 224, 280
Fnu4HI GCNGC 1 cut(s) 309
Fsp4HI GCNGC 1 cut(s) 309
FspBI CTAG 1 cut(s) 312
GluI GCNGC 1 cut(s) 309
HaeIII GGCC 2 cut(s) 46, 324
Hin1II CATG 2 cut(s) 228, 284
Hpy188I TCNGA 2 cut(s) 154, 335
HpyAV CCTTC 4 cut(s) 23, 193, 265, 335
HpyCH4IV ACGT 1 cut(s) 286
HpyCH4V TGCA 3 cut(s) 112, 139, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 1 cut(s) 286
Hsp92II CATG 2 cut(s) 228, 284
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 2 cut(s) 8, 159
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 2 cut(s) 85, 99
MaeI CTAG 1 cut(s) 312
MaeII ACGT 1 cut(s) 286
MalI GATC 1 cut(s) 242
MbiI CCGCTC 1 cut(s) 66
MboI GATC 1 cut(s) 240
MboII GAAGA 1 cut(s) 52
MluCI AATT 1 cut(s) 329
MmeI TCCRAC 2 cut(s) 93, 203
MnlI CCTC 2 cut(s) 148, 257
Mph1103I ATGCAT 1 cut(s) 114
MslI CAYNNNNRTG 2 cut(s) 81, 285
MwoI GCNNNNNNNGC 1 cut(s) 308
NdeII GATC 1 cut(s) 240
NlaIII CATG 2 cut(s) 228, 284
NsiI ATGCAT 1 cut(s) 114
PkrI GCNGC 1 cut(s) 310
PspPI GGNCC 1 cut(s) 176
RseI CAYNNNNRTG 2 cut(s) 81, 285
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 1 cut(s) 176
SetI ASST 6 cut(s) 27, 130, 204, 252, 268, 289
SfaNI GCATC 2 cut(s) 85, 99
SinI GGWCC 1 cut(s) 176
SmiMI CAYNNNNRTG 2 cut(s) 81, 285
Sse9I AATT 1 cut(s) 329
SsiI CCGC 1 cut(s) 64
SspMI CTAG 1 cut(s) 312
TaiI ACGT 1 cut(s) 289
TaqI TCGA 1 cut(s) 258
TaqII GACCGA 1 cut(s) 276
TasI AATT 1 cut(s) 329
TseI GCWGC 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 264
VpaK11BI GGWCC 1 cut(s) 176
XspI CTAG 1 cut(s) 312
Zsp2I ATGCAT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.