Rw4G024310

50S ribosomal protein L18

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0009178
Physical Location & Seq
Forward (+)
50159237 .. 50160737
1501 bp
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UTR
Exon/CDS
Intron
Rw4G024310.1

Sequence Viewer

Length: 480 bp
ATGTCAGCGTCTCTTTCGTTCATTGGGTCTTCACAACAGCTATGGATGCGTCCCAAGTTTGTAGGAGTATCCGCTCCCAGGCCTCAATCTCAGTCTCTAGTTATCGAAGCCAAGGCCACTACCAAAAGAGAAGACAGGACCGCTCGCCATACTCGTATCAGAAAGAAGGTTGAAGGAACTACCGAAAGGCCAAGACTATGTGTCTTCCGCTCCAACAAGCATCTCTATGTCCAAGTGATTGACGATACCAAGATGCATACACTTGCTTCAGCTTCAACAATGCAGAAGCCCATCTCTGAGGAGTTTGACTTCACTTCTGGTCCAACCATTGAAGTAGCAAAGAAGGTGGGAGAAGTCATTGCAAAGTCATGTTTGGAGAAAGGGATCACCAAGGTAGCCTTCGACCGAGGTGGCTATCCATATCATGGACGTGTGGAAGCCCTTGCTACTGCTGCTAGGGAGAATGGCCTTCAATTCTGA

Protein Analysis

159

Amino Acids

17.71

Weight (kDa)

9.9

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18p PF00861 41 - 159 9.1e-47 Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 425
AccBSI CCGCTC 3 cut(s) 74, 143, 210
AciI CCGC 3 cut(s) 72, 141, 208
AclWI GGATC 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 252
AfiI CCNNNNNNNGG 2 cut(s) 78, 425
AflIII ACRYGT 1 cut(s) 430
AgsI TTSAA 4 cut(s) 173, 276, 332, 473
AhdI GACNNNNNGTC 1 cut(s) 200
AjiI CACGTC 1 cut(s) 431
AjnI CCWGG 1 cut(s) 77
AjuI GAANNNNNNNTTGG 2 cut(s) 383, 415
AluBI AGCT 2 cut(s) 40, 272
AluI AGCT 2 cut(s) 40, 272
Alw26I GTCTC 2 cut(s) 15, 99
AlwI GGATC 1 cut(s) 392
AoxI GGCC 4 cut(s) 80, 114, 188, 466
ApeKI GCWGC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 138, 320
AsuHPI GGTGA 1 cut(s) 379
AvaII GGWCC 2 cut(s) 138, 320
BbsI GAAGAC 3 cut(s) 21, 138, 196
BbvI GCAGC 1 cut(s) 439
BccI CCATC 1 cut(s) 299
BciT130I CCWGG 1 cut(s) 79
BciVI GTATCC 1 cut(s) 79
BcoDI GTCTC 2 cut(s) 15, 99
BfaI CTAG 2 cut(s) 98, 456
BfuI GTATCC 1 cut(s) 79
BisI GCNGC 1 cut(s) 453
BlsI GCNGC 1 cut(s) 454
Bme1390I CCNGG 1 cut(s) 79
Bme18I GGWCC 2 cut(s) 138, 320
BmeRI GACNNNNNGTC 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 138, 320
BmrFI CCNGG 1 cut(s) 79
BmsI GCATC 3 cut(s) 36, 229, 243
BpiI GAAGAC 3 cut(s) 21, 138, 196
BsaJI CCNNGG 4 cut(s) 77, 111, 390, 406
Bsc4I CCNNNNNNNGG 2 cut(s) 78, 425
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 4 cut(s) 77, 111, 390, 406
BseGI GGATG 1 cut(s) 51
BseLI CCNNNNNNNGG 2 cut(s) 78, 425
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 2 cut(s) 104, 288
BseRI GAGGAG 1 cut(s) 314
BseXI GCAGC 1 cut(s) 439
Bsh1285I CGRYCG 1 cut(s) 406
BshFI GGCC 4 cut(s) 82, 116, 190, 468
BsiEI CGRYCG 1 cut(s) 406
BslFI GGGAC 1 cut(s) 36
BslI CCNNNNNNNGG 2 cut(s) 78, 425
BsmAI GTCTC 2 cut(s) 15, 99
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 1 cut(s) 36
BsnI GGCC 4 cut(s) 82, 116, 190, 468
Bsp143I GATC 1 cut(s) 384
BspACI CCGC 3 cut(s) 72, 141, 208
BspANI GGCC 4 cut(s) 82, 116, 190, 468
BspCNI CTCAG 2 cut(s) 103, 289
BspPI GGATC 1 cut(s) 392
BsrBI CCGCTC 3 cut(s) 74, 143, 210
BsrDI GCAATG 1 cut(s) 357
BssECI CCNNGG 4 cut(s) 77, 111, 390, 406
BssMI GATC 1 cut(s) 384
BssT1I CCWWGG 2 cut(s) 111, 390
Bst2UI CCWGG 1 cut(s) 79
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 2 cut(s) 90, 297
BstF5I GGATG 1 cut(s) 51
BstKTI GATC 1 cut(s) 387
BstMAI GTCTC 2 cut(s) 15, 99
BstMBI GATC 1 cut(s) 384
BstMCI CGRYCG 1 cut(s) 406
BstMWI GCNNNNNNNGC 2 cut(s) 46, 452
BstNI CCWGG 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 439
BstV2I GAAGAC 3 cut(s) 21, 138, 196
BsuI GTATCC 1 cut(s) 79
BsuRI GGCC 4 cut(s) 82, 116, 190, 468
BtrI CACGTC 1 cut(s) 431
BtsCI GGATG 1 cut(s) 51
Cac8I GCNNGC 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 138, 320
CseI GACGC 1 cut(s) 38
CspCI CAANNNNNGTGG 2 cut(s) 327, 362
CviAII CATG 2 cut(s) 369, 425
DdeI CTNAG 2 cut(s) 90, 297
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
DriI GACNNNNNGTC 1 cut(s) 200
Eam1105I GACNNNNNGTC 1 cut(s) 200
Eco130I CCWWGG 2 cut(s) 111, 390
Eco147I AGGCCT 1 cut(s) 82
Eco47I GGWCC 2 cut(s) 138, 320
Eco57I CTGAAG 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 77
EcoT14I CCWWGG 2 cut(s) 111, 390
EcoT22I ATGCAT 1 cut(s) 258
ErhI CCWWGG 2 cut(s) 111, 390
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 2 cut(s) 372, 428
FaiI YATR 8 cut(s) 43, 150, 199, 228, 258, 370, 421, 426
FalI AAGNNNNNCTT 2 cut(s) 383, 415
FaqI GGGAC 1 cut(s) 36
FatI CATG 2 cut(s) 368, 424
Fnu4HI GCNGC 1 cut(s) 453
FokI GGATG 1 cut(s) 58
Fsp4HI GCNGC 1 cut(s) 453
FspBI CTAG 2 cut(s) 98, 456
GluI GCNGC 1 cut(s) 453
HaeIII GGCC 4 cut(s) 82, 116, 190, 468
HgaI GACGC 1 cut(s) 38
Hin1II CATG 2 cut(s) 372, 428
HphI GGTGA 1 cut(s) 379
Hpy188I TCNGA 3 cut(s) 161, 298, 479
HpyAV CCTTC 5 cut(s) 160, 167, 337, 409, 479
HpyCH4IV ACGT 1 cut(s) 430
HpyCH4V TGCA 3 cut(s) 256, 283, 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 46, 452
HpyF3I CTNAG 2 cut(s) 90, 297
HpySE526I ACGT 1 cut(s) 430
Hsp92II CATG 2 cut(s) 372, 428
Kzo9I GATC 1 cut(s) 384
LmnI GCTCC 2 cut(s) 79, 215
LpnPI CCDG 4 cut(s) 64, 91, 121, 303
Lsp1109I GCAGC 1 cut(s) 439
LweI GCATC 3 cut(s) 36, 229, 243
MaeI CTAG 2 cut(s) 98, 456
MaeII ACGT 1 cut(s) 430
MalI GATC 1 cut(s) 386
MbiI CCGCTC 3 cut(s) 74, 143, 210
MboI GATC 1 cut(s) 384
MboII GAAGA 3 cut(s) 21, 143, 196
MluCI AATT 1 cut(s) 473
MmeI TCCRAC 2 cut(s) 237, 347
MnlI CCTC 3 cut(s) 93, 292, 401
Mph1103I ATGCAT 1 cut(s) 258
MslI CAYNNNNRTG 2 cut(s) 225, 429
MspR9I CCNGG 1 cut(s) 79
MvaI CCWGG 1 cut(s) 79
MwoI GCNNNNNNNGC 2 cut(s) 46, 452
NdeII GATC 1 cut(s) 384
NlaIII CATG 2 cut(s) 372, 428
NsiI ATGCAT 1 cut(s) 258
PceI AGGCCT 1 cut(s) 82
PcsI WCGNNNNNNNCGW 1 cut(s) 151
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 1 cut(s) 454
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspPI GGNCC 2 cut(s) 138, 320
RseI CAYNNNNRTG 2 cut(s) 225, 429
SatI GCNGC 1 cut(s) 453
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 2 cut(s) 138, 320
ScrFI CCNGG 1 cut(s) 79
SetI ASST 7 cut(s) 42, 171, 274, 348, 396, 412, 433
SfaNI GCATC 3 cut(s) 36, 229, 243
SinI GGWCC 2 cut(s) 138, 320
SmiMI CAYNNNNRTG 2 cut(s) 225, 429
Sse9I AATT 1 cut(s) 473
SseBI AGGCCT 1 cut(s) 82
SsiI CCGC 3 cut(s) 72, 141, 208
SspMI CTAG 2 cut(s) 98, 456
StuI AGGCCT 1 cut(s) 82
StyD4I CCNGG 1 cut(s) 77
StyI CCWWGG 2 cut(s) 111, 390
TaiI ACGT 1 cut(s) 433
TaqI TCGA 2 cut(s) 105, 402
TaqII GACCGA 1 cut(s) 420
TasI AATT 1 cut(s) 473
TseI GCWGC 1 cut(s) 452
TspDTI ATGAA 1 cut(s) 10
Van91I CCANNNNNTGG 1 cut(s) 425
VpaK11BI GGWCC 2 cut(s) 138, 320
XspI CTAG 2 cut(s) 98, 456
Zsp2I ATGCAT 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.