FvH4_4g25530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011343
Physical Location & Seq
Reverse (-)
27262332 .. 27264745
2414 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25530.t2

Sequence Viewer

Length: 1074 bp
ATGGCCGTAGTTCTCGGAAACCTAGCTCTGTTACTAGACGTGACCTCGCCCCGGACCATATTACCGGACCGGAAAACTCGTCCGGTGGCTCTTGACACTTTACTGAACCTAAACCTCAGCCTGCCGATAAAGCGGGAGAGGGACCCGCATAATCACAGTCACTCATTTAACGGCTTCATCGGCGCCAAGGGGTTTGACTCGGACGGCGGCGAGACTCGGAGCCAGCGAGTGGTGGCTCGGGGGAAGGCGAATTCGAAGGTGAACGGGGTGGACTTTGGGGAGGGGAACGGGAATGGATTTGGGGAAGGGGATGAGGAGGAGGAGCAGGCGGCGCCGTTGGATTGGGAGAAGGAAATGCGGCGGAGGGTGAAGGAGATCGAGGAGAGGAAGGAGCTGGAGAAGAAGGCTGAGGAGTTACAGAGCCGGATCGAGGAGGATTATGGAGGTGACGGTGGGGAAGAGACTGAGCAGGAGAAGCGGATGAGAGTTAGGAAAGAGCTAGAAAAGGTGGCTAAGGAGCAGGCTGAGCGGAGAGCCACCGCACAGTTAATGTTCGACTTGGGACAGAAGGCTTATGGGAGGGGGATGTATGGTCGAGCCATTGAGTTCCTAGAAGGTGCACTGACAATCATTCCGAGGCCAACGTTACTTGGTGGTGAGATACAAATTTGGCTTGCTATGGCTTACGAGGCTAACAAGCGCCATGCCGATTGCATTGATCTTTATAAGCAATTGGAGAACAAGCACCCCAGTGTCAGCATACGACGCCAAGCTGCAGATCTTCGCTACATTTTGCAAGCACCAAAGATCAAGATTACCCGAGAAGAGATGGTAACCATACCACTCATTGGTTCTAGTTATGACAGCTATGCTGGAACGTGGAGCGATAAATACAAGGACAAAGATCAGAGGAGCAGCGGGACAGTAACTAATCAAACTCCATCATCCAAAGACTATTTTGGGGACTTCATGGTATGGCGACCCCCAATTGGATTGGAGAAAAGCCAAGCTTTCTGGATAGCCTTGACATTATGGTTGGGTTTAGTTGGAGCTGCAGTCTTTCTTCAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

358

Amino Acids

40.25

Weight (kDa)

6.1

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26580
fragaria_vesca FvH4_4g25530 FvH4_4g25530
malus_domestica MD13G1101900.v1.1 MD16G1102400.v1.1
prunus_persica Prupe.1G243900_v2.0.a1 Prupe.1G243900_v2.0.a1
pyrus_communis pycom13g08900 pycom16g08700
rosa_chinensis RchiOBHm_Chr4g0432921
rosa_laevigata RLG00000006801
rosa_multiflora Rmu_sc0001762.1_g000047
rosa_roxburghii Rroxscaffold_5G00374330
rosa_rugosa Rorug04G0263800 Rorug04G0263900
rosa_samantha Rh4AG317900 Rh4BG326100 Rh4CG341200 Rh4DG321200
rosa_wichuraiana Rw4G027640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 726
AccB1I GGYRCC 2 cut(s) 182, 331
AccB7I CCANNNNNTGG 2 cut(s) 229, 848
AccBSI CCGCTC 1 cut(s) 529
AclI AACGTT 1 cut(s) 644
AclWI GGATC 1 cut(s) 434
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 250, 666
AcyI GRCGYC 3 cut(s) 183, 332, 766
AfiI CCNNNNNNNGG 5 cut(s) 51, 229, 430, 848, 989
AgsI TTSAA 1 cut(s) 1067
AjiI CACGTC 1 cut(s) 40
AleI CACNNNNGTG 1 cut(s) 750
AluBI AGCT 8 cut(s) 26, 394, 499, 773, 867, 1010, 1052, 1071
AluI AGCT 8 cut(s) 26, 394, 499, 773, 867, 1010, 1052, 1071
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 2 cut(s) 206, 455
Alw44I GTGCAC 1 cut(s) 618
AlwI GGATC 1 cut(s) 434
Ama87I CYCGRG 2 cut(s) 237, 819
AoxI GGCC 2 cut(s) 3, 638
ApaLI GTGCAC 1 cut(s) 618
ApeKI GCWGC 3 cut(s) 773, 915, 1052
ApoI RAATTY 2 cut(s) 250, 666
AspLEI GCGC 3 cut(s) 185, 334, 702
AspS9I GGNCC 3 cut(s) 54, 67, 142
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 4 cut(s) 271, 379, 458, 668
AsuII TTCGAA 1 cut(s) 254
AvaI CYCGRG 2 cut(s) 237, 819
AvaII GGWCC 3 cut(s) 54, 67, 142
BaeGI GKGCMC 1 cut(s) 622
BanI GGYRCC 2 cut(s) 182, 331
Bbv12I GWGCWC 1 cut(s) 622
BbvCI CCTCAGC 2 cut(s) 116, 408
BbvI GCAGC 3 cut(s) 760, 927, 1039
BccI CCATC 2 cut(s) 823, 949
BceAI ACGGC 3 cut(s) 187, 220, 319
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 2 cut(s) 206, 455
BfaI CTAG 5 cut(s) 23, 35, 500, 611, 855
BfmI CTRYAG 2 cut(s) 774, 1053
BfoI RGCGCY 3 cut(s) 186, 335, 703
BglII AGATCT 1 cut(s) 778
BisI GCNGC 6 cut(s) 208, 330, 359, 774, 916, 1053
BlpI GCTNAGC 1 cut(s) 525
BlsI GCNGC 6 cut(s) 209, 331, 360, 775, 917, 1054
Bme1390I CCNGG 1 cut(s) 52
Bme18I GGWCC 3 cut(s) 54, 67, 142
BmeT110I CYCGRG 2 cut(s) 237, 819
BmgBI CACGTC 1 cut(s) 40
BmgT120I GGNCC 3 cut(s) 54, 67, 142
BmiI GGNNCC 5 cut(s) 143, 144, 184, 221, 333
BmrFI CCNGG 1 cut(s) 52
BmrI ACTGGG 1 cut(s) 744
BmuI ACTGGG 1 cut(s) 744
BpmI CTGGAG 1 cut(s) 416
Bpu10I CCTNAGC 3 cut(s) 116, 408, 513
Bpu1102I GCTNAGC 1 cut(s) 525
Bpu14I TTCGAA 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 52
BsaHI GRCGYC 3 cut(s) 183, 332, 766
BsaJI CCNNGG 3 cut(s) 50, 186, 635
BsaWI WCCGGW 3 cut(s) 64, 69, 82
BsaXI ACNNNNNCTCC 2 cut(s) 1041, 1071
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 229, 430, 848, 989
Bse1I ACTGG 1 cut(s) 750
BseDI CCNNGG 3 cut(s) 50, 186, 635
BseGI GGATG 4 cut(s) 316, 486, 591, 944
BseLI CCNNNNNNNGG 5 cut(s) 51, 229, 430, 848, 989
BseMII CTCAG 4 cut(s) 130, 399, 456, 516
BseNI ACTGG 1 cut(s) 750
BseRI GAGGAG 7 cut(s) 329, 332, 335, 395, 425, 446, 925
BseSI GKGCMC 1 cut(s) 622
BseXI GCAGC 3 cut(s) 760, 927, 1039
BshFI GGCC 2 cut(s) 5, 640
BshNI GGYRCC 2 cut(s) 182, 331
BsiHKAI GWGCWC 1 cut(s) 622
BsiHKCI CYCGRG 2 cut(s) 237, 819
BsiSI CCGG 5 cut(s) 52, 65, 70, 83, 424
BslFI GGGAC 4 cut(s) 155, 576, 934, 977
BslI CCNNNNNNNGG 5 cut(s) 51, 229, 430, 848, 989
BsmAI GTCTC 2 cut(s) 206, 455
BsmFI GGGAC 4 cut(s) 155, 576, 934, 977
BsnI GGCC 2 cut(s) 5, 640
BsoBI CYCGRG 2 cut(s) 237, 819
Bsp119I TTCGAA 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 6 cut(s) 375, 426, 718, 778, 807, 904
Bsp1720I GCTNAGC 1 cut(s) 525
BspANI GGCC 2 cut(s) 5, 640
BspCNI CTCAG 4 cut(s) 129, 400, 457, 517
BspLI GGNNCC 5 cut(s) 143, 144, 184, 221, 333
BspMAI CTGCAG 2 cut(s) 778, 1057
BspPI GGATC 1 cut(s) 434
BspT104I TTCGAA 1 cut(s) 254
BspT107I GGYRCC 2 cut(s) 182, 331
BsrBI CCGCTC 1 cut(s) 529
BsrI ACTGG 1 cut(s) 750
BssECI CCNNGG 3 cut(s) 50, 186, 635
BssMI GATC 6 cut(s) 375, 426, 718, 778, 807, 904
BssNI GRCGYC 3 cut(s) 183, 332, 766
BssT1I CCWWGG 1 cut(s) 186
Bst4CI ACNGT 4 cut(s) 158, 452, 546, 925
Bst6I CTCTTC 2 cut(s) 453, 819
BstACI GRCGYC 3 cut(s) 183, 332, 766
BstBI TTCGAA 1 cut(s) 254
BstC8I GCNNGC 6 cut(s) 122, 224, 327, 522, 675, 798
BstDEI CTNAG 5 cut(s) 116, 408, 465, 513, 525
BstEII GGTNACC 1 cut(s) 832
BstF5I GGATG 4 cut(s) 316, 486, 591, 944
BstH2I RGCGCY 3 cut(s) 186, 335, 703
BstHHI GCGC 3 cut(s) 185, 334, 702
BstKTI GATC 6 cut(s) 378, 429, 721, 781, 810, 907
BstMAI GTCTC 2 cut(s) 206, 455
BstMBI GATC 6 cut(s) 375, 426, 718, 778, 807, 904
BstMWI GCNNNNNNNGC 7 cut(s) 130, 180, 331, 475, 526, 689, 765
BstPI GGTNACC 1 cut(s) 832
BstSCI CCNGG 1 cut(s) 50
BstSFI CTRYAG 2 cut(s) 774, 1053
BstSLI GKGCMC 1 cut(s) 622
BstV1I GCAGC 3 cut(s) 760, 927, 1039
BstX2I RGATCY 1 cut(s) 778
BstYI RGATCY 1 cut(s) 778
BsuRI GGCC 2 cut(s) 5, 640
BtrI CACGTC 1 cut(s) 40
BtsCI GGATG 4 cut(s) 316, 486, 591, 944
BtsIMutI CAGTG 2 cut(s) 620, 757
Cac8I GCNNGC 6 cut(s) 122, 224, 327, 522, 675, 798
CfoI GCGC 3 cut(s) 185, 334, 702
Cfr13I GGNCC 3 cut(s) 54, 67, 142
CpoI CGGWCCG 1 cut(s) 67
CseI GACGC 1 cut(s) 774
CspI CGGWCCG 1 cut(s) 67
CviAII CATG 2 cut(s) 704, 970
DdeI CTNAG 5 cut(s) 116, 408, 465, 513, 525
DinI GGCGCC 2 cut(s) 184, 333
DpnI GATC 6 cut(s) 377, 428, 720, 780, 809, 906
DpnII GATC 6 cut(s) 375, 426, 718, 778, 807, 904
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 453, 819
EarI CTCTTC 2 cut(s) 453, 819
EciI GGCGGA 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 186
Eco47I GGWCC 3 cut(s) 54, 67, 142
Eco88I CYCGRG 2 cut(s) 237, 819
Eco91I GGTNACC 1 cut(s) 832
EcoO109I RGGNCCY 1 cut(s) 142
EcoO65I GGTNACC 1 cut(s) 832
EcoRI GAATTC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 186
EgeI GGCGCC 2 cut(s) 184, 333
EheI GGCGCC 2 cut(s) 184, 333
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 2 cut(s) 707, 973
FalI AAGNNNNNCTT 2 cut(s) 994, 1026
FaqI GGGAC 4 cut(s) 155, 576, 934, 977
FatI CATG 2 cut(s) 703, 969
FauI CCCGC 3 cut(s) 126, 153, 911
Fnu4HI GCNGC 6 cut(s) 208, 330, 359, 774, 916, 1053
FokI GGATG 4 cut(s) 323, 493, 598, 931
Fsp4HI GCNGC 6 cut(s) 208, 330, 359, 774, 916, 1053
FspBI CTAG 5 cut(s) 23, 35, 500, 611, 855
GlaI GCGC 3 cut(s) 184, 333, 701
GluI GCNGC 6 cut(s) 208, 330, 359, 774, 916, 1053
GsuI CTGGAG 1 cut(s) 416
HaeII RGCGCY 3 cut(s) 186, 335, 703
HaeIII GGCC 2 cut(s) 5, 640
HapII CCGG 5 cut(s) 52, 65, 70, 83, 424
HgaI GACGC 1 cut(s) 774
HhaI GCGC 3 cut(s) 185, 334, 702
Hin1I GRCGYC 3 cut(s) 183, 332, 766
Hin1II CATG 2 cut(s) 707, 973
Hin6I GCGC 3 cut(s) 183, 332, 700
HinP1I GCGC 3 cut(s) 183, 332, 700
HindIII AAGCTT 1 cut(s) 1008
HinfI GANTC 2 cut(s) 197, 214
HpaII CCGG 5 cut(s) 52, 65, 70, 83, 424
HphI GGTGA 4 cut(s) 271, 379, 458, 668
Hpy166II GTNNAC 3 cut(s) 262, 271, 620
Hpy188I TCNGA 5 cut(s) 17, 202, 219, 636, 909
Hpy188III TCNNGA 3 cut(s) 92, 811, 1015
Hpy8I GTNNAC 3 cut(s) 262, 271, 620
Hpy99I CGWCG 1 cut(s) 768
HpyAV CCTTC 9 cut(s) 238, 250, 299, 343, 364, 382, 397, 562, 608
HpyCH4III ACNGT 4 cut(s) 158, 452, 546, 925
HpyCH4IV ACGT 3 cut(s) 39, 644, 878
HpyCH4V TGCA 5 cut(s) 620, 714, 776, 796, 1055
HpyF10VI GCNNNNNNNGC 7 cut(s) 130, 180, 331, 475, 526, 689, 765
HpyF3I CTNAG 5 cut(s) 116, 408, 465, 513, 525
HpySE526I ACGT 3 cut(s) 39, 644, 878
Hsp92I GRCGYC 3 cut(s) 183, 332, 766
Hsp92II CATG 2 cut(s) 707, 973
HspAI GCGC 3 cut(s) 183, 332, 700
KasI GGCGCC 2 cut(s) 182, 331
KflI GGGWCCC 1 cut(s) 142
Kzo9I GATC 6 cut(s) 375, 426, 718, 778, 807, 904
LmnI GCTCC 7 cut(s) 219, 322, 391, 517, 882, 912, 1049
Lsp1109I GCAGC 3 cut(s) 760, 927, 1039
MaeI CTAG 5 cut(s) 23, 35, 500, 611, 855
MaeII ACGT 3 cut(s) 39, 644, 878
MaeIII GTNAC 8 cut(s) 30, 40, 158, 414, 446, 645, 832, 925
MalI GATC 6 cut(s) 377, 428, 720, 780, 809, 906
MbiI CCGCTC 1 cut(s) 529
MboI GATC 6 cut(s) 375, 426, 718, 778, 807, 904
MboII GAAGA 5 cut(s) 412, 470, 773, 836, 1055
MfeI CAATTG 2 cut(s) 731, 987
MflI RGATCY 1 cut(s) 778
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 4 cut(s) 250, 666, 731, 987
Mly113I GGCGCC 2 cut(s) 183, 332
MlyI GAGTC 2 cut(s) 191, 208
MmeI TCCRAC 2 cut(s) 318, 1027
MseI TTAA 2 cut(s) 168, 548
MslI CAYNNNNRTG 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 918
MspI CCGG 5 cut(s) 52, 65, 70, 83, 424
MspR9I CCNGG 1 cut(s) 52
MunI CAATTG 2 cut(s) 731, 987
MwoI GCNNNNNNNGC 7 cut(s) 130, 180, 331, 475, 526, 689, 765
NarI GGCGCC 2 cut(s) 183, 332
NciI CCSGG 1 cut(s) 52
NdeII GATC 6 cut(s) 375, 426, 718, 778, 807, 904
NlaIII CATG 2 cut(s) 707, 973
NlaIV GGNNCC 5 cut(s) 143, 144, 184, 221, 333
NmuCI GTSAC 3 cut(s) 40, 158, 446
NspV TTCGAA 1 cut(s) 254
OliI CACNNNNGTG 1 cut(s) 750
PcsI WCGNNNNNNNCGW 1 cut(s) 223
PflMI CCANNNNNTGG 2 cut(s) 229, 848
PkrI GCNGC 6 cut(s) 209, 331, 360, 775, 917, 1054
PleI GAGTC 2 cut(s) 191, 208
PluTI GGCGCC 2 cut(s) 186, 335
PpsI GAGTC 2 cut(s) 191, 208
PpuMI RGGWCCY 1 cut(s) 142
PsiI TTATAA 1 cut(s) 726
Psp1406I AACGTT 1 cut(s) 644
Psp5II RGGWCCY 1 cut(s) 142
PspEI GGTNACC 1 cut(s) 832
PspN4I GGNNCC 5 cut(s) 143, 144, 184, 221, 333
PspPI GGNCC 3 cut(s) 54, 67, 142
PspPPI RGGWCCY 1 cut(s) 142
PstI CTGCAG 2 cut(s) 778, 1057
PsuI RGATCY 1 cut(s) 778
RseI CAYNNNNRTG 1 cut(s) 750
Rsr2I CGGWCCG 1 cut(s) 67
RsrII CGGWCCG 1 cut(s) 67
SaqAI TTAA 2 cut(s) 168, 548
SatI GCNGC 6 cut(s) 208, 330, 359, 774, 916, 1053
Sau3AI GATC 6 cut(s) 375, 426, 718, 778, 807, 904
Sau96I GGNCC 3 cut(s) 54, 67, 142
SchI GAGTC 2 cut(s) 191, 208
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 1 cut(s) 622
SfcI CTRYAG 2 cut(s) 774, 1053
SfoI GGCGCC 2 cut(s) 184, 333
SfuI TTCGAA 1 cut(s) 254
SinI GGWCC 3 cut(s) 54, 67, 142
SmiMI CAYNNNNRTG 1 cut(s) 750
Sse9I AATT 4 cut(s) 250, 666, 731, 987
SspDI GGCGCC 2 cut(s) 182, 331
SspMI CTAG 5 cut(s) 23, 35, 500, 611, 855
StyD4I CCNGG 1 cut(s) 50
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 158, 452, 546, 925
TaiI ACGT 3 cut(s) 42, 647, 881
TaqI TCGA 5 cut(s) 254, 378, 429, 555, 595
TasI AATT 4 cut(s) 250, 666, 731, 987
TauI GCSGC 3 cut(s) 210, 332, 361
Tru1I TTAA 2 cut(s) 168, 548
Tru9I TTAA 2 cut(s) 168, 548
TscAI CASTG 2 cut(s) 627, 757
TseFI GTSAC 3 cut(s) 40, 158, 446
TseI GCWGC 3 cut(s) 773, 915, 1052
Tsp45I GTSAC 3 cut(s) 40, 158, 446
TspDTI ATGAA 2 cut(s) 166, 958
TspRI CASTG 2 cut(s) 627, 757
Van91I CCANNNNNTGG 2 cut(s) 229, 848
VneI GTGCAC 1 cut(s) 618
VpaK11BI GGWCC 3 cut(s) 54, 67, 142
XapI RAATTY 2 cut(s) 250, 666
XspI CTAG 5 cut(s) 23, 35, 500, 611, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.