Rorug04G0263800

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001929
Physical Location & Seq
Reverse (-)
43541010 .. 43542112
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0263800.1

Sequence Viewer

Length: 300 bp
ATGGCTTCCTGTCTCTCAAAAGCAAATCTCCTTCCCCTTGCCGCCTCCATTCATCGACGAGGTTACGCGGCTGCATCACATGGCCCAGTGGCAGCTGGATATGGAATGGCAGGGTCCAGGAGTGCTATGGTGAGGAAGCTGGAAGAGGGGGTTGGGATGAAGGAAGAGTCTTCGTCTGCTTGGGCACCGGATCCTCGAACCGGGTACTACAGGCCGGCTAATAGGGGGGATGAGATCGACCCAGTGGAGCTCCGGGAAATGCTCTTGAACCAGAAACAGTTGTTGGTGAAATTACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

99

Amino Acids

10.7

Weight (kDa)

9.25

Isoelectric Point (pI)

52.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_3a PF03242 3 - 93 6.3e-26 Late embryogenesis abundant protein (LEA_3a subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 184
AccII CGCG 1 cut(s) 68
AciI CCGC 2 cut(s) 42, 68
AclWI GGATC 2 cut(s) 185, 198
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 1 cut(s) 268
AjnI CCWGG 1 cut(s) 116
AjuI GAANNNNNNNTTGG 4 cut(s) 135, 167, 266, 298
AluBI AGCT 3 cut(s) 95, 139, 250
AluI AGCT 3 cut(s) 95, 139, 250
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 1 cut(s) 17
AlwI GGATC 2 cut(s) 185, 198
AoxI GGCC 2 cut(s) 82, 212
ApeKI GCWGC 2 cut(s) 71, 92
AspS9I GGNCC 2 cut(s) 83, 114
AsuC2I CCSGG 2 cut(s) 202, 254
AsuHPI GGTGA 2 cut(s) 142, 298
AvaII GGWCC 1 cut(s) 114
BaeGI GKGCMC 1 cut(s) 187
BamHI GGATCC 1 cut(s) 190
BanI GGYRCC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 252
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 2 cut(s) 58, 104
BciT130I CCWGG 1 cut(s) 118
BcnI CCSGG 2 cut(s) 202, 254
BcoDI GTCTC 1 cut(s) 17
BfmI CTRYAG 1 cut(s) 208
BisI GCNGC 4 cut(s) 42, 69, 72, 93
BlsI GCNGC 4 cut(s) 43, 70, 73, 94
Bme1390I CCNGG 3 cut(s) 118, 202, 254
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 2 cut(s) 83, 114
BmiI GGNNCC 3 cut(s) 115, 186, 192
BmrFI CCNGG 3 cut(s) 118, 202, 254
BmrI ACTGGG 2 cut(s) 80, 236
BmsI GCATC 1 cut(s) 83
BmuI ACTGGG 2 cut(s) 80, 236
BpiI GAAGAC 1 cut(s) 162
BpuMI CCSGG 2 cut(s) 202, 254
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 2 cut(s) 86, 242
BseBI CCWGG 1 cut(s) 118
BseGI GGATG 2 cut(s) 162, 235
BseLI CCNNNNNNNGG 1 cut(s) 200
BseNI ACTGG 2 cut(s) 86, 242
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 2 cut(s) 58, 104
Bsh1236I CGCG 1 cut(s) 68
BshFI GGCC 2 cut(s) 84, 214
BshNI GGYRCC 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 252
BsiSI CCGG 4 cut(s) 188, 201, 215, 253
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 1 cut(s) 17
BsnI GGCC 2 cut(s) 84, 214
Bsp1286I GDGCHC 2 cut(s) 187, 252
Bsp143I GATC 2 cut(s) 190, 234
BspACI CCGC 2 cut(s) 42, 68
BspANI GGCC 2 cut(s) 84, 214
BspFNI CGCG 1 cut(s) 68
BspLI GGNNCC 3 cut(s) 115, 186, 192
BspPI GGATC 2 cut(s) 185, 198
BspT107I GGYRCC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 2 cut(s) 86, 242
BssAI RCCGGY 1 cut(s) 214
BssMI GATC 2 cut(s) 190, 234
Bst2UI CCWGG 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 279
Bst6I CTCTTC 2 cut(s) 138, 159
BstC8I GCNNGC 1 cut(s) 216
BstF5I GGATG 2 cut(s) 162, 235
BstFNI CGCG 1 cut(s) 68
BstKTI GATC 2 cut(s) 193, 237
BstMAI GTCTC 1 cut(s) 17
BstMBI GATC 2 cut(s) 190, 234
BstNI CCWGG 1 cut(s) 118
BstSCI CCNGG 3 cut(s) 116, 200, 252
BstSFI CTRYAG 1 cut(s) 208
BstSLI GKGCMC 1 cut(s) 187
BstUI CGCG 1 cut(s) 68
BstV1I GCAGC 2 cut(s) 58, 104
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
BsuRI GGCC 2 cut(s) 84, 214
BtsCI GGATG 2 cut(s) 162, 235
BtsIMutI CAGTG 2 cut(s) 93, 249
Cac8I GCNNGC 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 2 cut(s) 83, 114
Csp6I GTAC 1 cut(s) 205
CviAII CATG 1 cut(s) 80
CviJI RGCY 8 cut(s) 5, 71, 84, 95, 139, 214, 218, 250
CviKI_1 RGCY 8 cut(s) 5, 71, 84, 95, 139, 214, 218, 250
CviQI GTAC 1 cut(s) 205
DpnI GATC 2 cut(s) 192, 236
DpnII GATC 2 cut(s) 190, 234
Eam1104I CTCTTC 2 cut(s) 138, 159
EarI CTCTTC 2 cut(s) 138, 159
Ecl136II GAGCTC 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 114
Eco53kI GAGCTC 1 cut(s) 250
EcoICRI GAGCTC 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 116
EcoT38I GRGCYC 1 cut(s) 252
FaeI CATG 1 cut(s) 83
FaiI YATR 3 cut(s) 81, 102, 128
FatI CATG 1 cut(s) 79
Fnu4HI GCNGC 4 cut(s) 42, 69, 72, 93
FokI GGATG 2 cut(s) 169, 242
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 4 cut(s) 42, 69, 72, 93
GluI GCNGC 4 cut(s) 42, 69, 72, 93
HaeIII GGCC 2 cut(s) 84, 214
HapII CCGG 4 cut(s) 188, 201, 215, 253
Hin1II CATG 1 cut(s) 83
HinfI GANTC 1 cut(s) 167
HpaII CCGG 4 cut(s) 188, 201, 215, 253
HphI GGTGA 2 cut(s) 142, 298
Hpy188III TCNNGA 1 cut(s) 265
Hpy99I CGWCG 1 cut(s) 60
HpyAV CCTTC 2 cut(s) 41, 154
HpyCH4III ACNGT 1 cut(s) 279
HpyCH4V TGCA 1 cut(s) 74
Hsp92II CATG 1 cut(s) 83
KroI GCCGGC 1 cut(s) 214
KroNI GCCGGC 1 cut(s) 216
Kzo9I GATC 2 cut(s) 190, 234
LmnI GCTCC 2 cut(s) 247, 255
Lsp1109I GCAGC 2 cut(s) 58, 104
LweI GCATC 1 cut(s) 83
MaeIII GTNAC 1 cut(s) 62
MalI GATC 2 cut(s) 192, 236
MboI GATC 2 cut(s) 190, 234
MboII GAAGA 3 cut(s) 155, 162, 176
MflI RGATCY 1 cut(s) 190
MhlI GDGCHC 2 cut(s) 187, 252
MluCI AATT 1 cut(s) 290
MlyI GAGTC 1 cut(s) 176
MnlI CCTC 5 cut(s) 53, 55, 126, 139, 204
MroNI GCCGGC 1 cut(s) 214
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 4 cut(s) 188, 201, 215, 253
MspR9I CCNGG 3 cut(s) 118, 202, 254
MvaI CCWGG 1 cut(s) 118
MvnI CGCG 1 cut(s) 68
NaeI GCCGGC 1 cut(s) 216
NciI CCSGG 2 cut(s) 202, 254
NdeII GATC 2 cut(s) 190, 234
NgoMIV GCCGGC 1 cut(s) 214
NlaIII CATG 1 cut(s) 83
NlaIV GGNNCC 3 cut(s) 115, 186, 192
PdiI GCCGGC 1 cut(s) 216
PfoI TCCNGGA 2 cut(s) 116, 252
PkrI GCNGC 4 cut(s) 43, 70, 73, 94
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 252
Psp6I CCWGG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 115, 186, 192
PspPI GGNCC 2 cut(s) 83, 114
PsuI RGATCY 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SacI GAGCTC 1 cut(s) 252
SatI GCNGC 4 cut(s) 42, 69, 72, 93
Sau3AI GATC 2 cut(s) 190, 234
Sau96I GGNCC 2 cut(s) 83, 114
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 3 cut(s) 118, 202, 254
SduI GDGCHC 2 cut(s) 187, 252
SetI ASST 4 cut(s) 64, 97, 141, 252
SfaNI GCATC 1 cut(s) 83
SfcI CTRYAG 1 cut(s) 208
SinI GGWCC 1 cut(s) 114
Sse9I AATT 1 cut(s) 290
SsiI CCGC 2 cut(s) 42, 68
SstI GAGCTC 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 116, 200, 252
TaaI ACNGT 1 cut(s) 279
TaqI TCGA 3 cut(s) 55, 196, 237
TasI AATT 1 cut(s) 290
TauI GCSGC 2 cut(s) 44, 71
TscAI CASTG 2 cut(s) 93, 249
TseI GCWGC 2 cut(s) 71, 92
TspDTI ATGAA 2 cut(s) 41, 173
TspRI CASTG 2 cut(s) 93, 249
VpaK11BI GGWCC 1 cut(s) 114
XcmI CCANNNNNNNNNTGG 1 cut(s) 124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.