Rorug05G0573700

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001929
Physical Location & Seq
Reverse (-)
76888672 .. 76894126
5455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0573700.1

Sequence Viewer

Length: 4023 bp
ATGTCGGACTATCTTCACTCTCCGGCTGTAGAGCCGTTTAGCCAGCAGTCGCTGCAGCCGCGGCTCCAGGCGCTGATCGAGGGCGCCAGGGAGAGCTGGACCTATGCCATCTTCTGGCAGTCGTCGTACGACATGTCGGGAGATTCCGTTCTCGGGTGGGGCGACGGGTTCTACAAGGACGAGAGTGATAAATGGAAAGCGAGGCCCAAAAAGATGACGTCATTAGTCGAGCAGGAGTACCGGAAGAAAGTGTTCAGGGACCTCAACTCTTTGATTTCCGGGGCCGACACCACCGCCGACGACGAGGTCGTAGATCAAGTCACTGGTACAGAATGGTTCTTCCTTGTTTCCATGACCCATAGTTTCGTCAACGGCGGCGGGTTGCCGGGTCAGGCCTTTTTCTATTCCAACCCGGTCTGGGTCACCGGGCCGGACCGACTAGCGGCGTCCTCCTGCGAGCGGGCCCGCCAAGCTCAGGTCTCCGGCCTACAGACTATGGTCTGCGTCCCGACGGCGATTGGGGTGGTCGAATTGGGGTCGACGGAGCTGATATTCCTGAGCTCGGATGTGATGAACGAGGCCCGAGTATTGTTTGATTTCAATAATCTTGAAGTCGGCTCTTGGCCTGTAGCTCCGGCCACTTCAGGACCGAGTAGTAGTAACCATCACATCTCTAAGCCGATTCAGTTTGACAACCACCCTAGTTCCAGTAGCTCTTCTGCTACTCAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGAAGGAGTATTGTATCTGATTTATACGATCGGCTGCAAAACAAGAGAGGAATTAGAACATTTATGGATGATCCAGATCTTCGAGTAGGGGATGCTATTTCTCCCGCTCTCTTAAAGGCGATTGAAGAATCAAGGTTTGCAATCGTTGTTTTATCGAAAAAGTATGCTCATTCTACTTGGTGTTTGGAGGAACTTACAAGTATTTGTCAGTGCATGAAAGACTTCAACAGAATTCTTCCACTTTTCTATAATGTGGAACCCTCTGATGTAAGGTACCGGAAAAGGAGTTTCCGAAGAGCTTTCGATAAGCATGAACGCTCTGGGCGACACACATCAGAGAAGGTGCAGCGGTGGAATGATGCGTTAAACAAAGTGGCCAGTTTTTCTGGGTGGCATACAAAAAATTTTAAGACTGATAGAGATCTTGTCGACTCCATTGTGGAATTTGTGTGCAATGAAGTACAGCCTATTGAAATCGGGTTTACAATGTTCGCCGGAGATTTTCAAGCATTTGAAGCAACAACAAAAAGCATGAATGAGGTTATGAAGGCGCTTCAAGATGACGAGGTCACTGCCCTTGGGGTCTATGGAATGGGAGGGGTCGGCAAGACAACCATGGTTACACGTGTTGGTGAGCTAGCCCGCAAGAATGGAATCTTTAACCATGTACTTCTGGCTGCCATATCCCAAAGCCCTGACTCGAGAAATATTCAAGCCACATTGGCAGAGCTGCTGGGCTTCAAATTCCAGAGGGATACAGAAATTGGAAGAGCCGCTGAGTTGCATAAGAAGATAATGAGTAAAAAGAAGATCCTTATAATCCTGGACGACATTTGGGAGAGAATGGATTTGTCAAGAATAGGAATTCCCAGCTATGACGAGCTCAAAAAATACAATTCTAAAGTCCTACTCACCACGAGGAGATTGCATGTTTGTAATTCCATGGACAGCCAAGCAAGCATTCCTCTCGATATCCTATCAGAAGAAGATTCTTGGAAATTGTTTATGAGAAATGCAGGAAGGTCTTTTGAATCCCCCAATTTTAATAATGTAGCAAGAAAGGTAGCTGGAGAATGTAGAGGTCTACCAATTGCATTGATAGCAGTTGCAAGGGCACTCAGAGATAAAGATCTGGCGGAATGGCAAAAAGCAGCCCAACGACTAGAGAAGTCACAAATTGCCAACCCTGATGATAAGGGAGATGCATTCGAATGTATAAAATTAAGCTTTGATTACTTGAAAGATGAGGACCACAAGTCATGCTTCTTGCTCTGTTGCCTATTCCCAGAAGACCATGAAATCAATATAGAAGAATTGTTCAGGTATGCGATTGGAAAAGGATTGTTTCAAGATGCCGAAACAATGGACGAAGCCAGAGGAACAGCAGATTCAGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGAGAGTGAATACATTGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATGAATATTGCAAAATCTAAAAATGGGCATCGGTTTACGGTAAAAGTTGGCTGTGGTTTAGAGGATTGGCGGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACAAAATTCGCAAGCTACCCGAAGAAGAGTTGGTATGTCCAAATCTCCAGCTTTTATTACTAAAACAGAATGCTGATTTAAATGAGATCCCAGAAAAGTTGATCCAAAATCTGAATAAATTAAGGCTCTTGGATCTTAGCAACACTAGTATTTCCGTATTACCCCAATCATTCAGTCTCCTTACCAACCTTCAAGCTTTGAATTTAGATTTTTGCGAGCAATTGATTGATATATCCGTAGTGGGAAAACTTAAGAAGCTTGAAATTCTTAGTATGAGACAATGTCCTCTCAAGAAACTGTCGAGAGAAATAGGACATTTGACCAATCTAAGAATTTTGGATGTCAGTTCCGCTGAATTTCTCATAATTCCACCTAAAGTGATACCGAAGTTGCATAAATTAGAAGAACTGTATATACTAAACTGTGGATTTGAGGATTGGGGGAGTGAAGTTGAGAGAGAAGGAGAAGAAACTGAATTGGTGTTCCCTGTTTTGTCAAATTTGAAAAATTGGCAGGTTTGCATATCTGATGCAAAATGCGAACCTATCAGTCTTGAGGTCAATGGACAATATCATAATTCAAGATCCTTGTTTCTTAATGGAGCAACCATAAGTACCTTACCTGATTGGTTTATCAACACAGTGACAAAGAAAACAGAGAAGCTAGAGTACAGTCGCTGCAAAGGGATGAGTGACATTCTTATGGAATATGACCATGGGAGGTTACATAAGCTCAAGCATCTCAGAGTATGTGGTTATTTTTGTGATTCCTATGTGTACTTGAAGGAGTTGATGAACACAACAAGACGAGTTCAAAAAGGACCAGTGTTTGAGAATTTGGAAGAGTTGCATCTGATAGATTTGATCCACCTTAATGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTTCAATCTCAAGGTATTGCATGTGTCTTTTTGTAATATTTTGAAGAATGTATCAAAATTGGTACAGAGACTACCAAATCTGGAGAAACTATATTTAAATTGCATGGATAACTTGGAATATGTGTTTGGATGCGAAGGGTTTGAGCCGAAAGAATCAAAACTGAGAGAGATGCAGTTGTTGGGTCCAAATTCAGTGAAGAGCATTTGTAGTGGTCCTGCCCCACGCGTAATGTTCCAAAGTCTTAAAAGTTTGACCTTTTACCGTTGCGAGTTGCTGCAAAGTCTGTTCGTATATGATGTAGCCCAGAATCTTGTTCAATTGGAAGACCTTTTAGTAGAATGGTGCCCTTTGTTGGAAAGAGTAATTGAAGCAGTGAACAACGAGAAGACGGTTCTTCGAAAAATGAAGAACTTGGTTTTCATGGGACTTCCCAAGTTGTATGGTGCAAGTGCTACTATTGACATTGAGTGTCCTTCATTGGAACACTTGATTGTGGTGGATTGTCCCCGGTTTTCATTTTCAACCTCTTCCTTAAGCAGGAACCAGTTTTCATTTTCAACCCCTGCTTCTGACTACTTTGGCAGCAGGAACCTAGTCAAACTCAATGATCAACAACATCTTCAACTTCTATCATCAAGGGTTGGTCATCTAGGTGAGGCTGCAAATCCGATCTATACGCTTGAAGCTGCATTGCTGGATCGTGACTTTCAAGACAGCCACAGGGATTATAACAGCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

1340

Amino Acids

152.06

Weight (kDa)

6.06

Isoelectric Point (pI)

43.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 18 - 198 1.4e-50 bHLH-MYC and R2R3-MYB transcription factors N-terminal
TIR PF01582 245 - 411 1e-45 TIR domain
TIR_2 PF13676 248 - 345 1.6e-16 TIR domain
NB-ARC PF00931 432 - 597 1.1e-29 NB-ARC domain
LRR_8 PF13855 804 - 862 8.8e-07 Leucine rich repeat
LRR_14 PF23598 806 - 1003 2.3e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 1070 - 1209 5.4e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1149 - 1262 3.8e-09 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1589, 4011
AasI GACNNNNNNGTC 1 cut(s) 305
AatII GACGTC 1 cut(s) 221
Acc36I ACCTGC 1 cut(s) 2875
Acc65I GGTACC 1 cut(s) 1044
AccB1I GGYRCC 3 cut(s) 83, 1044, 3624
AccB7I CCANNNNNTGG 1 cut(s) 114
AccBSI CCGCTC 2 cut(s) 460, 878
AccI GTMKAC 3 cut(s) 539, 1200, 1855
AccII CGCG 3 cut(s) 61, 2331, 3507
AclWI GGATC 8 cut(s) 836, 1576, 2452, 2467, 2511, 2949, 3229, 3987
AcoI YGGCCR 3 cut(s) 636, 1146, 2324
AcuI CTGAAG 1 cut(s) 627
AcvI CACGTG 1 cut(s) 1397
AcyI GRCGYC 3 cut(s) 84, 218, 446
AfiI CCNNNNNNNGG 9 cut(s) 114, 153, 418, 442, 459, 475, 562, 3634, 3819
AflII CTTAAG 2 cut(s) 2621, 3814
AflIII ACRYGT 4 cut(s) 132, 1394, 1396, 3505
AhdI GACNNNNNGTC 1 cut(s) 2026
AhlI ACTAGT 1 cut(s) 2516
AjnI CCWGG 4 cut(s) 66, 86, 1593, 3271
AjuI GAANNNNNNNTTGG 4 cut(s) 1716, 1748, 3390, 3422
AloI GAACNNNNNNTCC 2 cut(s) 132, 164
Alw21I GWGCWC 2 cut(s) 563, 1656
Alw26I GTCTC 5 cut(s) 484, 2552, 2641, 3282, 3343
AlwI GGATC 8 cut(s) 836, 1576, 2452, 2467, 2511, 2949, 3229, 3987
AlwNI CAGNNNCTG 3 cut(s) 52, 73, 3048
Ama87I CYCGRG 3 cut(s) 152, 582, 1471
ApaI GGGCCC 1 cut(s) 466
Asp700I GAANNNNTTC 2 cut(s) 251, 992
Asp718I GGTACC 1 cut(s) 1044
AspLEI GCGC 3 cut(s) 73, 86, 1324
AsuC2I CCSGG 6 cut(s) 280, 387, 413, 427, 2243, 3790
AsuHPI GGTGA 5 cut(s) 415, 1415, 1675, 3272, 3947
AsuII TTCGAA 2 cut(s) 1980, 3679
AsuNHI GCTAGC 1 cut(s) 1408
AvaI CYCGRG 3 cut(s) 152, 582, 1471
AvaII GGWCC 9 cut(s) 99, 259, 433, 647, 761, 2020, 3191, 3464, 3494
BaeGI GKGCMC 3 cut(s) 466, 1888, 3629
BaeI ACNNNNGTAYC 2 cut(s) 2744, 2777
BalI TGGCCA 1 cut(s) 1148
BanI GGYRCC 3 cut(s) 83, 1044, 3624
BanII GRGCYC 3 cut(s) 466, 563, 1656
BarI GAAGNNNNNNTAC 2 cut(s) 3023, 3055
BauI CACGAG 1 cut(s) 1687
BbrPI CACGTG 1 cut(s) 1397
BbsI GAAGAC 3 cut(s) 2067, 3612, 3674
Bbv12I GWGCWC 2 cut(s) 563, 1656
BccI CCATC 2 cut(s) 116, 672
BceAI ACGGC 3 cut(s) 19, 388, 528
BcgI CGANNNNNNTGC 6 cut(s) 1325, 1359, 1678, 1712, 1720, 1754
BciT130I CCWGG 4 cut(s) 68, 88, 1595, 3273
BciVI GTATCC 2 cut(s) 1519, 2239
BclI TGATCA 1 cut(s) 3889
BcnI CCSGG 6 cut(s) 280, 387, 413, 427, 2243, 3790
BcoDI GTCTC 5 cut(s) 484, 2552, 2641, 3282, 3343
BcuI ACTAGT 1 cut(s) 2516
BfaI CTAG 9 cut(s) 440, 702, 1409, 1934, 2187, 2517, 3035, 3875, 3932
BfmI CTRYAG 4 cut(s) 27, 53, 488, 627
BfoI RGCGCY 3 cut(s) 74, 87, 1325
BfrI CTTAAG 2 cut(s) 2621, 3814
BfuAI ACCTGC 1 cut(s) 2875
BfuI GTATCC 2 cut(s) 1519, 2239
BglI GCCNNNNNGGC 1 cut(s) 1493
BglII AGATCT 3 cut(s) 847, 1192, 1900
Bme18I GGWCC 9 cut(s) 99, 259, 433, 647, 761, 2020, 3191, 3464, 3494
BmeRI GACNNNNNGTC 1 cut(s) 2026
BmeT110I CYCGRG 3 cut(s) 152, 582, 1471
BmtI GCTAGC 1 cut(s) 1412
BoxI GACNNNNGTC 1 cut(s) 497
BpiI GAAGAC 3 cut(s) 2067, 3612, 3674
BpmI CTGGAG 4 cut(s) 50, 1860, 2402, 3383
Bpu10I CCTNAGC 2 cut(s) 474, 557
Bpu14I TTCGAA 2 cut(s) 1980, 3679
BpuEI CTTGAG 4 cut(s) 2645, 2945, 3089, 3275
BpuMI CCSGG 6 cut(s) 280, 387, 413, 427, 2243, 3790
BsaAI YACGTR 1 cut(s) 1397
BsaBI GATNNNNATC 3 cut(s) 846, 1191, 1899
BsaHI GRCGYC 3 cut(s) 84, 218, 446
BsaI GGTCTC 2 cut(s) 484, 3282
BsaJI CCNNGG 9 cut(s) 59, 87, 279, 1348, 1386, 1713, 3085, 3272, 3788
BsaWI WCCGGW 2 cut(s) 240, 1047
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 9 cut(s) 114, 153, 418, 442, 459, 475, 562, 3634, 3819
Bse1I ACTGG 5 cut(s) 328, 708, 1149, 3194, 3826
Bse3DI GCAATG 2 cut(s) 1231, 3971
Bse8I GATNNNNATC 3 cut(s) 846, 1191, 1899
BseBI CCWGG 4 cut(s) 68, 88, 1595, 3273
BseDI CCNNGG 9 cut(s) 59, 87, 279, 1348, 1386, 1713, 3085, 3272, 3788
BseGI GGATG 8 cut(s) 571, 844, 868, 2222, 2238, 2716, 3063, 3416
BseJI GATNNNNATC 3 cut(s) 846, 1191, 1899
BseLI CCNNNNNNNGG 9 cut(s) 114, 153, 418, 442, 459, 475, 562, 3634, 3819
BseMI GCAATG 2 cut(s) 1231, 3971
BseMII CTCAG 7 cut(s) 488, 548, 740, 1539, 1903, 3127, 3434
BseNI ACTGG 5 cut(s) 328, 708, 1149, 3194, 3826
BseRI GAGGAG 1 cut(s) 1705
BseSI GKGCMC 3 cut(s) 466, 1888, 3629
BseYI CCCAGC 2 cut(s) 1504, 1640
BsgI GTGCAG 2 cut(s) 1136, 2332
Bsh1236I CGCG 3 cut(s) 61, 2331, 3507
Bsh1285I CGRYCG 1 cut(s) 802
BshNI GGYRCC 3 cut(s) 83, 1044, 3624
BsiEI CGRYCG 1 cut(s) 802
BsiHKAI GWGCWC 2 cut(s) 563, 1656
BsiHKCI CYCGRG 3 cut(s) 152, 582, 1471
BsiWI CGTACG 1 cut(s) 126
BslFI GGGAC 4 cut(s) 272, 491, 3720, 3771
BslI CCNNNNNNNGG 9 cut(s) 114, 153, 418, 442, 459, 475, 562, 3634, 3819
BsmAI GTCTC 5 cut(s) 484, 2552, 2641, 3282, 3343
BsmFI GGGAC 4 cut(s) 272, 491, 3720, 3771
BsmI GAATGC 3 cut(s) 1731, 1976, 2446
Bso31I GGTCTC 2 cut(s) 484, 3282
BsoBI CYCGRG 3 cut(s) 152, 582, 1471
Bsp119I TTCGAA 2 cut(s) 1980, 3679
Bsp120I GGGCCC 1 cut(s) 462
Bsp1286I GDGCHC 5 cut(s) 466, 563, 1656, 1888, 3629
Bsp19I CCATGG 3 cut(s) 1386, 1713, 3085
BspCNI CTCAG 7 cut(s) 487, 549, 739, 1540, 1902, 3126, 3435
BspFNI CGCG 3 cut(s) 61, 2331, 3507
BspMAI CTGCAG 1 cut(s) 57
BspMI ACCTGC 1 cut(s) 2875
BspOI GCTAGC 1 cut(s) 1412
BspPI GGATC 8 cut(s) 836, 1576, 2452, 2467, 2511, 2949, 3229, 3987
BspQI GCTCTTC 4 cut(s) 721, 1060, 1534, 3473
BspT104I TTCGAA 2 cut(s) 1980, 3679
BspT107I GGYRCC 3 cut(s) 83, 1044, 3624
BspTI CTTAAG 2 cut(s) 2621, 3814
BspTNI GGTCTC 2 cut(s) 484, 3282
BsrBI CCGCTC 2 cut(s) 460, 878
BsrDI GCAATG 2 cut(s) 1231, 3971
BsrI ACTGG 5 cut(s) 328, 708, 1149, 3194, 3826
BssECI CCNNGG 9 cut(s) 59, 87, 279, 1348, 1386, 1713, 3085, 3272, 3788
BssNI GRCGYC 3 cut(s) 84, 218, 446
BssSI CACGAG 1 cut(s) 1687
BssT1I CCWWGG 4 cut(s) 1348, 1386, 1713, 3085
Bst2BI CACGAG 1 cut(s) 1687
Bst2UI CCWGG 4 cut(s) 68, 88, 1595, 3273
Bst4CI ACNGT 8 cut(s) 2293, 2670, 2781, 2795, 3013, 3044, 3545, 3673
Bst6I CTCTTC 8 cut(s) 721, 1060, 1534, 2391, 3207, 3287, 3473, 3814
BstACI GRCGYC 3 cut(s) 84, 218, 446
BstAFI CTTAAG 2 cut(s) 2621, 3814
BstAPI GCANNNNNTGC 1 cut(s) 52
BstBAI YACGTR 1 cut(s) 1397
BstBI TTCGAA 2 cut(s) 1980, 3679
BstDSI CCRYGG 4 cut(s) 59, 1386, 1713, 3085
BstEII GGTNACC 1 cut(s) 421
BstENI CCTNNNNNAGG 1 cut(s) 3817
BstF5I GGATG 8 cut(s) 571, 844, 868, 2222, 2238, 2716, 3063, 3416
BstFNI CGCG 3 cut(s) 61, 2331, 3507
BstH2I RGCGCY 3 cut(s) 74, 87, 1325
BstHHI GCGC 3 cut(s) 73, 86, 1324
BstMAI GTCTC 5 cut(s) 484, 2552, 2641, 3282, 3343
BstMCI CGRYCG 1 cut(s) 802
BstMWI GCNNNNNNNGC 9 cut(s) 52, 58, 61, 70, 470, 1286, 1493, 1728, 1871
BstNI CCWGG 4 cut(s) 68, 88, 1595, 3273
BstNSI RCATGY 3 cut(s) 136, 1703, 3305
BstPAI GACNNNNGTC 1 cut(s) 497
BstPI GGTNACC 1 cut(s) 421
BstSFI CTRYAG 4 cut(s) 27, 53, 488, 627
BstSLI GKGCMC 3 cut(s) 466, 1888, 3629
BstUI CGCG 3 cut(s) 61, 2331, 3507
BstV2I GAAGAC 3 cut(s) 2067, 3612, 3674
BstX2I RGATCY 7 cut(s) 847, 1192, 1581, 1900, 2457, 2503, 2954
BstYI RGATCY 7 cut(s) 847, 1192, 1581, 1900, 2457, 2503, 2954
BsuI GTATCC 2 cut(s) 1519, 2239
BtgI CCRYGG 4 cut(s) 59, 1386, 1713, 3085
BtsCI GGATG 8 cut(s) 571, 844, 868, 2222, 2238, 2716, 3063, 3416
BtsI GCAGTG 3 cut(s) 1341, 2349, 3660
BveI ACCTGC 1 cut(s) 2875
CaiI CAGNNNCTG 3 cut(s) 52, 73, 3048
CfoI GCGC 3 cut(s) 73, 86, 1324
Cfr42I CCGCGG 1 cut(s) 62
CpoI CGGWCCG 1 cut(s) 433
CseI GACGC 2 cut(s) 435, 493
CspI CGGWCCG 1 cut(s) 433
DinI GGCGCC 1 cut(s) 85
DraI TTTAAA 2 cut(s) 2451, 3378
DrdI GACNNNNNNGTC 1 cut(s) 305
DriI GACNNNNNGTC 1 cut(s) 2026
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 3 cut(s) 636, 1146, 2324
Eam1104I CTCTTC 8 cut(s) 721, 1060, 1534, 2391, 3207, 3287, 3473, 3814
Eam1105I GACNNNNNGTC 1 cut(s) 2026
EarI CTCTTC 8 cut(s) 721, 1060, 1534, 2391, 3207, 3287, 3473, 3814
EciI GGCGGA 1 cut(s) 1922
Ecl136II GAGCTC 2 cut(s) 561, 1654
Eco130I CCWWGG 4 cut(s) 1348, 1386, 1713, 3085
Eco147I AGGCCT 1 cut(s) 395
Eco24I GRGCYC 3 cut(s) 466, 563, 1656
Eco31I GGTCTC 2 cut(s) 484, 3282
Eco32I GATATC 1 cut(s) 1744
Eco47I GGWCC 9 cut(s) 99, 259, 433, 647, 761, 2020, 3191, 3464, 3494
Eco53kI GAGCTC 2 cut(s) 561, 1654
Eco57I CTGAAG 1 cut(s) 627
Eco72I CACGTG 1 cut(s) 1397
Eco88I CYCGRG 3 cut(s) 152, 582, 1471
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 2 cut(s) 561, 1654
EcoNI CCTNNNNNAGG 1 cut(s) 3817
EcoO109I RGGNCCY 2 cut(s) 259, 761
EcoO65I GGTNACC 1 cut(s) 421
EcoRI GAATTC 2 cut(s) 1002, 1635
EcoRII CCWGG 4 cut(s) 66, 86, 1593, 3271
EcoRV GATATC 1 cut(s) 1744
EcoT14I CCWWGG 4 cut(s) 1348, 1386, 1713, 3085
EcoT22I ATGCAT 2 cut(s) 1978, 2232
EcoT38I GRGCYC 3 cut(s) 466, 563, 1656
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
ErhI CCWWGG 4 cut(s) 1348, 1386, 1713, 3085
FalI AAGNNNNNCTT 2 cut(s) 2018, 2050
FaqI GGGAC 4 cut(s) 272, 491, 3720, 3771
FauI CCCGC 5 cut(s) 371, 453, 473, 883, 1421
FbaI TGATCA 1 cut(s) 3889
FblI GTMKAC 3 cut(s) 539, 1200, 1855
FokI GGATG 8 cut(s) 578, 851, 875, 2225, 2229, 2723, 3070, 3423
FriOI GRGCYC 3 cut(s) 466, 563, 1656
FspBI CTAG 9 cut(s) 440, 702, 1409, 1934, 2187, 2517, 3035, 3875, 3932
GlaI GCGC 3 cut(s) 72, 85, 1323
GsaI CCCAGC 2 cut(s) 1508, 1644
GsuI CTGGAG 4 cut(s) 50, 1860, 2402, 3383
HaeII RGCGCY 3 cut(s) 74, 87, 1325
HgaI GACGC 2 cut(s) 435, 493
HhaI GCGC 3 cut(s) 73, 86, 1324
Hin1I GRCGYC 3 cut(s) 84, 218, 446
Hin6I GCGC 3 cut(s) 71, 84, 1322
HinP1I GCGC 3 cut(s) 71, 84, 1322
HincII GTYRAC 3 cut(s) 370, 540, 1201
HindII GTYRAC 3 cut(s) 370, 540, 1201
HindIII AAGCTT 3 cut(s) 1996, 2565, 2627
HphI GGTGA 5 cut(s) 415, 1415, 1675, 3272, 3947
Hpy166II GTNNAC 8 cut(s) 370, 540, 1201, 1254, 1856, 2289, 3148, 3658
Hpy8I GTNNAC 8 cut(s) 370, 540, 1201, 1254, 1856, 2289, 3148, 3658
Hpy99I CGWCG 6 cut(s) 127, 167, 302, 305, 514, 544
HpyCH4III ACNGT 8 cut(s) 2293, 2670, 2781, 2795, 3013, 3044, 3545, 3673
HpyCH4IV ACGT 2 cut(s) 218, 1396
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 58, 61, 70, 470, 1286, 1493, 1728, 1871
HpySE526I ACGT 2 cut(s) 218, 1396
Hsp92I GRCGYC 3 cut(s) 84, 218, 446
HspAI GCGC 3 cut(s) 71, 84, 1322
KasI GGCGCC 1 cut(s) 83
KpnI GGTACC 1 cut(s) 1048
Ksp22I TGATCA 1 cut(s) 3889
KspI CCGCGG 1 cut(s) 62
LguI GCTCTTC 4 cut(s) 721, 1060, 1534, 3473
LmnI GCTCC 4 cut(s) 69, 544, 637, 2972
MaeI CTAG 9 cut(s) 440, 702, 1409, 1934, 2187, 2517, 3035, 3875, 3932
MaeII ACGT 2 cut(s) 218, 1396
MbiI CCGCTC 2 cut(s) 460, 878
MfeI CAATTG 3 cut(s) 1860, 2591, 3599
MflI RGATCY 7 cut(s) 847, 1192, 1581, 1900, 2457, 2503, 2954
MhlI GDGCHC 5 cut(s) 466, 563, 1656, 1888, 3629
MlsI TGGCCA 1 cut(s) 1148
MluI ACGCGT 1 cut(s) 3505
MluNI TGGCCA 1 cut(s) 1148
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 2 cut(s) 1196, 1463
MmeI TCCRAC 2 cut(s) 432, 3615
Mox20I TGGCCA 1 cut(s) 1148
Mph1103I ATGCAT 2 cut(s) 1978, 2232
MroXI GAANNNNTTC 2 cut(s) 251, 992
MscI TGGCCA 1 cut(s) 1148
MslI CAYNNNNRTG 4 cut(s) 1981, 3071, 3243, 3933
Msp20I TGGCCA 1 cut(s) 1148
MspA1I CMGCKG 4 cut(s) 61, 1120, 1547, 2723
MspCI CTTAAG 2 cut(s) 2621, 3814
MunI CAATTG 3 cut(s) 1860, 2591, 3599
Mva1269I GAATGC 3 cut(s) 1731, 1976, 2446
MvaI CCWGG 4 cut(s) 68, 88, 1595, 3273
MvnI CGCG 3 cut(s) 61, 2331, 3507
MwoI GCNNNNNNNGC 9 cut(s) 52, 58, 61, 70, 470, 1286, 1493, 1728, 1871
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 6 cut(s) 280, 387, 413, 427, 2243, 3790
NcoI CCATGG 3 cut(s) 1386, 1713, 3085
NheI GCTAGC 1 cut(s) 1408
NmuCI GTSAC 7 cut(s) 319, 421, 1339, 1941, 3013, 3062, 3983
NsiI ATGCAT 2 cut(s) 1978, 2232
NspI RCATGY 3 cut(s) 136, 1703, 3305
NspV TTCGAA 2 cut(s) 1980, 3679
PaeR7I CTCGAG 1 cut(s) 1471
PceI AGGCCT 1 cut(s) 395
PciI ACATGT 1 cut(s) 132
PciSI GCTCTTC 4 cut(s) 721, 1060, 1534, 3473
PcsI WCGNNNNNNNCGW 3 cut(s) 159, 306, 1093
PctI GAATGC 3 cut(s) 1731, 1976, 2446
PdmI GAANNNNTTC 2 cut(s) 251, 992
Pfl23II CGTACG 1 cut(s) 126
PflFI GACNNNGTC 3 cut(s) 305, 1337, 2652
PflMI CCANNNNNTGG 1 cut(s) 114
PfoI TCCNGGA 2 cut(s) 1593, 2241
Ple19I CGATCG 1 cut(s) 802
PleI GAGTC 2 cut(s) 1196, 1463
PluTI GGCGCC 1 cut(s) 87
PmaCI CACGTG 1 cut(s) 1397
PmlI CACGTG 1 cut(s) 1397
PpsI GAGTC 2 cut(s) 1196, 1463
Ppu21I YACGTR 1 cut(s) 1397
PpuMI RGGWCCY 2 cut(s) 259, 761
PscI ACATGT 1 cut(s) 132
PshAI GACNNNNGTC 1 cut(s) 497
PsiI TTATAA 2 cut(s) 1589, 4011
Psp124BI GAGCTC 2 cut(s) 563, 1656
Psp5II RGGWCCY 2 cut(s) 259, 761
Psp6I CCWGG 4 cut(s) 66, 86, 1593, 3271
PspCI CACGTG 1 cut(s) 1397
PspEI GGTNACC 1 cut(s) 421
PspFI CCCAGC 2 cut(s) 1504, 1640
PspGI CCWGG 4 cut(s) 66, 86, 1593, 3271
PspLI CGTACG 1 cut(s) 126
PspOMI GGGCCC 1 cut(s) 462
PspPPI RGGWCCY 2 cut(s) 259, 761
PsrI GAACNNNNNNTAC 2 cut(s) 2769, 2801
PstI CTGCAG 1 cut(s) 57
PstNI CAGNNNCTG 3 cut(s) 52, 73, 3048
PsuI RGATCY 7 cut(s) 847, 1192, 1581, 1900, 2457, 2503, 2954
PsyI GACNNNGTC 3 cut(s) 305, 1337, 2652
PvuI CGATCG 1 cut(s) 802
RseI CAYNNNNRTG 4 cut(s) 1981, 3071, 3243, 3933
Rsr2I CGGWCCG 1 cut(s) 433
RsrII CGGWCCG 1 cut(s) 433
SacI GAGCTC 2 cut(s) 563, 1656
SacII CCGCGG 1 cut(s) 62
SalI GTCGAC 2 cut(s) 538, 1199
SapI GCTCTTC 4 cut(s) 721, 1060, 1534, 3473
SchI GAGTC 2 cut(s) 1196, 1463
SduI GDGCHC 5 cut(s) 466, 563, 1656, 1888, 3629
SfcI CTRYAG 4 cut(s) 27, 53, 488, 627
SfoI GGCGCC 1 cut(s) 85
Sfr274I CTCGAG 1 cut(s) 1471
Sfr303I CCGCGG 1 cut(s) 62
SfuI TTCGAA 2 cut(s) 1980, 3679
SgrBI CCGCGG 1 cut(s) 62
SinI GGWCC 9 cut(s) 99, 259, 433, 647, 761, 2020, 3191, 3464, 3494
SlaI CTCGAG 1 cut(s) 1471
SmiI ATTTAAAT 2 cut(s) 2451, 3378
SmiMI CAYNNNNRTG 4 cut(s) 1981, 3071, 3243, 3933
SmlI CTYRAG 7 cut(s) 1471, 2621, 2660, 2924, 3104, 3290, 3814
SmoI CTYRAG 7 cut(s) 1471, 2621, 2660, 2924, 3104, 3290, 3814
SpeI ACTAGT 1 cut(s) 2516
SseBI AGGCCT 1 cut(s) 395
SspDI GGCGCC 1 cut(s) 83
SspI AATATT 3 cut(s) 1480, 2260, 3319
SspMI CTAG 9 cut(s) 440, 702, 1409, 1934, 2187, 2517, 3035, 3875, 3932
SstI GAGCTC 2 cut(s) 563, 1656
StuI AGGCCT 1 cut(s) 395
StyI CCWWGG 4 cut(s) 1348, 1386, 1713, 3085
SwaI ATTTAAAT 2 cut(s) 2451, 3378
TaaI ACNGT 8 cut(s) 2293, 2670, 2781, 2795, 3013, 3044, 3545, 3673
TaiI ACGT 2 cut(s) 221, 1399
TaqII GACCGA 2 cut(s) 450, 664
TatI WGTACW 4 cut(s) 1231, 1438, 3039, 3147
TauI GCSGC 6 cut(s) 61, 64, 378, 446, 1547, 2326
TseFI GTSAC 7 cut(s) 319, 421, 1339, 1941, 3013, 3062, 3983
Tsp45I GTSAC 7 cut(s) 319, 421, 1339, 1941, 3013, 3062, 3983
TspGWI ACGGA 4 cut(s) 136, 557, 2515, 2596
Tth111I GACNNNGTC 3 cut(s) 305, 1337, 2652
Van91I CCANNNNNTGG 1 cut(s) 114
Vha464I CTTAAG 2 cut(s) 2621, 3814
VpaK11BI GGWCC 9 cut(s) 99, 259, 433, 647, 761, 2020, 3191, 3464, 3494
XagI CCTNNNNNAGG 1 cut(s) 3817
XceI RCATGY 3 cut(s) 136, 1703, 3305
XhoI CTCGAG 1 cut(s) 1471
XmiI GTMKAC 3 cut(s) 539, 1200, 1855
XmnI GAANNNNTTC 2 cut(s) 251, 992
XspI CTAG 9 cut(s) 440, 702, 1409, 1934, 2187, 2517, 3035, 3875, 3932
ZraI GACGTC 1 cut(s) 219
Zsp2I ATGCAT 2 cut(s) 1978, 2232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.