Rroxscaffold_7G00208570

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001929
Physical Location & Seq
Reverse (-)
58619852 .. 58637981
18130 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00208570.1

Sequence Viewer

Length: 930 bp
ATGGAAACGACAGCCAAAAGAGGAGTGAGCAAACCAAAGTCTACTGTAGTCCCCGTTGCCAAAGAAATAAAGAAACAGTCTGCAGCTGAACTAGCTTCTTCAGGAATTACACATGTTTGGCTTCCACCACCTTCTCATGCTTGTTCAGATGAAGGTGGTTCACCTAGCATTATTCCACCATATATACTGAACTCAACTTTTTTTGAAGGATACCTGCCTGGTAGGCTCAATGATCTCACATCAAAATACGGAAGCCAAGATGAATTAAAGGCACCGGTAAAAACCTACCATGACAATGGGATACAGAGCATAGCAGATATTGTTATAAACCACGGATGTGCAGAGAAGAAAGATGAGAGAGGAATATTGTGCATTTTTGAAGGAGTAACACCGATGATCGCCTTGATTGGGGCCCGTCTCTTGTTTGCAGCAACGACGAGAGAGTTTTCGATGGCAAGGGAAACCCCGATACCGGAAATAGCCGGGGTGGGTGAGTGGCCATTTCCAACAGATAAAAGTCATTCAAGGAATGCTTACATTCTTACTCATCTAAGGATCCCATCAATTTTCTACGACCACTTCTTTGACTGGGAATGGGACTGGGACTGGGGCTTGAGAGTGGAGATCTCGAAATTCACTGCGATTATGTCCAGGAATGGGATCAAACTTTACGGTGTGGTGCGTATCGTAGCAGCTGATCCAGATCTCTATGTAGCTTCCACAGATGACAAAATCATAATGAAGATTGGTCCAAGATATGAGGTTGGGGACCTTGAAGCAAGTCGAGCAAAACAAACTATTTTGAGCAAGCGGCTAAGGAGTAGAGCCGCTGTAGCTGAGTGTGAGTGGAGTCGGAAGAGCTACAAGACACGTTTTGGGAGGTGTGGCGATCGTTGTTTTGATCGGCGTAGTGGAAAAGCAAACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

309

Amino Acids

34.53

Weight (kDa)

8.91

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amyl_C2 PF07821 218 - 261 1e-09 Alpha-amylase C-terminal beta-sheet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 222
AccB1I GGYRCC 1 cut(s) 271
AccI GTMKAC 1 cut(s) 41
AciI CCGC 2 cut(s) 811, 828
AclWI GGATC 4 cut(s) 550, 563, 668, 692
AcoI YGGCCR 1 cut(s) 497
AcsI RAATTY 1 cut(s) 632
AcuI CTGAAG 1 cut(s) 84
AfiI CCNNNNNNNGG 3 cut(s) 408, 472, 657
AflIII ACRYGT 2 cut(s) 112, 869
AgeI ACCGGT 1 cut(s) 274
AgsI TTSAA 4 cut(s) 206, 380, 525, 776
AjnI CCWGG 2 cut(s) 217, 650
AleI CACNNNNGTG 1 cut(s) 336
AluBI AGCT 6 cut(s) 86, 95, 695, 716, 836, 861
AluI AGCT 6 cut(s) 86, 95, 695, 716, 836, 861
Alw26I GTCTC 1 cut(s) 422
AlwI GGATC 4 cut(s) 550, 563, 668, 692
AoxI GGCC 2 cut(s) 411, 497
ApaI GGGCCC 1 cut(s) 415
ApeKI GCWGC 3 cut(s) 83, 428, 692
ApoI RAATTY 1 cut(s) 632
ArsI GACNNNNNNTTYG 2 cut(s) 566, 598
AsiGI ACCGGT 1 cut(s) 274
AspS9I GGNCC 4 cut(s) 411, 412, 749, 769
AsuC2I CCSGG 1 cut(s) 484
AsuHPI GGTGA 2 cut(s) 153, 503
AvaII GGWCC 2 cut(s) 749, 769
BaeGI GKGCMC 1 cut(s) 415
BalI TGGCCA 1 cut(s) 499
BamHI GGATCC 1 cut(s) 555
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 1 cut(s) 415
BbvI GCAGC 3 cut(s) 95, 440, 704
BccI CCATC 2 cut(s) 445, 568
BciT130I CCWGG 2 cut(s) 219, 652
BciVI GTATCC 2 cut(s) 203, 294
BcnI CCSGG 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 422
BfaI CTAG 2 cut(s) 92, 165
BfmI CTRYAG 3 cut(s) 45, 81, 831
BfuAI ACCTGC 1 cut(s) 222
BfuI GTATCC 2 cut(s) 203, 294
BglI GCCNNNNNGGC 1 cut(s) 223
BglII AGATCT 2 cut(s) 624, 703
BisI GCNGC 5 cut(s) 84, 429, 693, 812, 828
BlsI GCNGC 5 cut(s) 85, 430, 694, 813, 829
Bme1390I CCNGG 3 cut(s) 219, 484, 652
Bme18I GGWCC 2 cut(s) 749, 769
BmgT120I GGNCC 4 cut(s) 411, 412, 749, 769
BmiI GGNNCC 5 cut(s) 273, 412, 413, 557, 770
BmrFI CCNGG 3 cut(s) 219, 484, 652
BmrI ACTGGG 3 cut(s) 598, 610, 616
BmuI ACTGGG 3 cut(s) 598, 610, 616
Bpu10I CCTNAGC 1 cut(s) 815
BpuEI CTTGAG 1 cut(s) 634
BpuMI CCSGG 1 cut(s) 484
BsaBI GATNNNNATC 1 cut(s) 702
BsaJI CCNNGG 2 cut(s) 331, 483
BsaWI WCCGGW 2 cut(s) 274, 472
Bsc4I CCNNNNNNNGG 3 cut(s) 408, 472, 657
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 3 cut(s) 593, 605, 611
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 2 cut(s) 219, 652
BseDI CCNNGG 2 cut(s) 331, 483
BseGI GGATG 1 cut(s) 341
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 3 cut(s) 408, 472, 657
BseMII CTCAG 1 cut(s) 828
BseNI ACTGG 3 cut(s) 593, 605, 611
BseRI GAGGAG 1 cut(s) 36
BseSI GKGCMC 1 cut(s) 415
BseXI GCAGC 3 cut(s) 95, 440, 704
BsgI GTGCAG 1 cut(s) 360
Bsh1285I CGRYCG 1 cut(s) 892
BshFI GGCC 2 cut(s) 413, 499
BshNI GGYRCC 1 cut(s) 271
BshTI ACCGGT 1 cut(s) 274
BsiEI CGRYCG 1 cut(s) 892
BsiSI CCGG 3 cut(s) 275, 473, 483
BslFI GGGAC 4 cut(s) 35, 611, 617, 782
BslI CCNNNNNNNGG 3 cut(s) 408, 472, 657
BsmAI GTCTC 1 cut(s) 422
BsmBI CGTCTC 1 cut(s) 422
BsmFI GGGAC 4 cut(s) 35, 611, 617, 782
BsmI GAATGC 1 cut(s) 535
BsnI GGCC 2 cut(s) 413, 499
Bsp120I GGGCCC 1 cut(s) 411
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 9 cut(s) 232, 396, 555, 624, 660, 697, 703, 889, 901
BspACI CCGC 2 cut(s) 811, 828
BspANI GGCC 2 cut(s) 413, 499
BspCNI CTCAG 1 cut(s) 829
BspLI GGNNCC 5 cut(s) 273, 412, 413, 557, 770
BspMAI CTGCAG 1 cut(s) 85
BspMI ACCTGC 1 cut(s) 222
BspPI GGATC 4 cut(s) 550, 563, 668, 692
BspQI GCTCTTC 1 cut(s) 851
BspT107I GGYRCC 1 cut(s) 271
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 3 cut(s) 593, 605, 611
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 2 cut(s) 331, 483
BssMI GATC 9 cut(s) 232, 396, 555, 624, 660, 697, 703, 889, 901
Bst2UI CCWGG 2 cut(s) 219, 652
Bst4CI ACNGT 3 cut(s) 46, 78, 674
Bst6I CTCTTC 1 cut(s) 851
BstC8I GCNNGC 1 cut(s) 809
BstDEI CTNAG 3 cut(s) 551, 815, 837
BstDSI CCRYGG 1 cut(s) 331
BstF5I GGATG 1 cut(s) 341
BstKTI GATC 9 cut(s) 235, 399, 558, 627, 663, 700, 706, 892, 904
BstMAI GTCTC 1 cut(s) 422
BstMBI GATC 9 cut(s) 232, 396, 555, 624, 660, 697, 703, 889, 901
BstMCI CGRYCG 1 cut(s) 892
BstMWI GCNNNNNNNGC 4 cut(s) 92, 223, 785, 833
BstNI CCWGG 2 cut(s) 219, 652
BstNSI RCATGY 1 cut(s) 116
BstSCI CCNGG 3 cut(s) 217, 482, 650
BstSFI CTRYAG 3 cut(s) 45, 81, 831
BstSLI GKGCMC 1 cut(s) 415
BstV1I GCAGC 3 cut(s) 95, 440, 704
BstX2I RGATCY 3 cut(s) 555, 624, 703
BstXI CCANNNNNNTGG 1 cut(s) 296
BstYI RGATCY 3 cut(s) 555, 624, 703
BsuI GTATCC 2 cut(s) 203, 294
BsuRI GGCC 2 cut(s) 413, 499
BtgI CCRYGG 1 cut(s) 331
BtsCI GGATG 1 cut(s) 341
BtsI GCAGTG 1 cut(s) 636
BtsIMutI CAGTG 1 cut(s) 636
BveI ACCTGC 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 809
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 4 cut(s) 411, 412, 749, 769
CspAI ACCGGT 1 cut(s) 274
CviAII CATG 3 cut(s) 113, 137, 290
DdeI CTNAG 3 cut(s) 551, 815, 837
DpnI GATC 9 cut(s) 234, 398, 557, 626, 662, 699, 705, 891, 903
DpnII GATC 9 cut(s) 232, 396, 555, 624, 660, 697, 703, 889, 901
EaeI YGGCCR 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 851
EarI CTCTTC 1 cut(s) 851
Eco24I GRGCYC 1 cut(s) 415
Eco47I GGWCC 2 cut(s) 749, 769
Eco57I CTGAAG 1 cut(s) 84
EcoO109I RGGNCCY 2 cut(s) 411, 769
EcoRII CCWGG 2 cut(s) 217, 650
EcoT38I GRGCYC 1 cut(s) 415
Esp3I CGTCTC 1 cut(s) 422
FaeI CATG 3 cut(s) 116, 140, 293
FalI AAGNNNNNCTT 2 cut(s) 517, 549
FaqI GGGAC 4 cut(s) 35, 611, 617, 782
FatI CATG 3 cut(s) 112, 136, 289
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 5 cut(s) 84, 429, 693, 812, 828
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 5 cut(s) 84, 429, 693, 812, 828
FspBI CTAG 2 cut(s) 92, 165
GluI GCNGC 5 cut(s) 84, 429, 693, 812, 828
HaeIII GGCC 2 cut(s) 413, 499
HapII CCGG 3 cut(s) 275, 473, 483
Hin1II CATG 3 cut(s) 116, 140, 293
HinfI GANTC 1 cut(s) 850
HpaII CCGG 3 cut(s) 275, 473, 483
HphI GGTGA 2 cut(s) 153, 503
Hpy166II GTNNAC 2 cut(s) 42, 161
Hpy188I TCNGA 2 cut(s) 148, 855
Hpy188III TCNNGA 3 cut(s) 102, 628, 701
Hpy8I GTNNAC 2 cut(s) 42, 161
Hpy99I CGWCG 1 cut(s) 439
HpyAV CCTTC 4 cut(s) 141, 146, 200, 374
HpyCH4III ACNGT 3 cut(s) 46, 78, 674
HpyCH4IV ACGT 1 cut(s) 871
HpyCH4V TGCA 4 cut(s) 83, 341, 372, 428
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 223, 785, 833
HpyF3I CTNAG 3 cut(s) 551, 815, 837
HpySE526I ACGT 1 cut(s) 871
Hsp92II CATG 3 cut(s) 116, 140, 293
Kzo9I GATC 9 cut(s) 232, 396, 555, 624, 660, 697, 703, 889, 901
LguI GCTCTTC 1 cut(s) 851
Lsp1109I GCAGC 3 cut(s) 95, 440, 704
MaeI CTAG 2 cut(s) 92, 165
MaeII ACGT 1 cut(s) 871
MaeIII GTNAC 1 cut(s) 385
MalI GATC 9 cut(s) 234, 398, 557, 626, 662, 699, 705, 891, 903
MboI GATC 9 cut(s) 232, 396, 555, 624, 660, 697, 703, 889, 901
MboII GAAGA 4 cut(s) 90, 358, 754, 868
MflI RGATCY 3 cut(s) 555, 624, 703
MhlI GDGCHC 1 cut(s) 415
MlsI TGGCCA 1 cut(s) 499
MluCI AATT 4 cut(s) 105, 263, 564, 632
MluNI TGGCCA 1 cut(s) 499
MlyI GAGTC 1 cut(s) 859
MmeI TCCRAC 2 cut(s) 530, 833
MnlI CCTC 4 cut(s) 14, 353, 754, 873
Mox20I TGGCCA 1 cut(s) 499
MscI TGGCCA 1 cut(s) 499
MseI TTAA 2 cut(s) 266, 928
MslI CAYNNNNRTG 2 cut(s) 294, 336
Msp20I TGGCCA 1 cut(s) 499
MspA1I CMGCKG 3 cut(s) 86, 695, 830
MspI CCGG 3 cut(s) 275, 473, 483
MspR9I CCNGG 3 cut(s) 219, 484, 652
Mva1269I GAATGC 1 cut(s) 535
MvaI CCWGG 2 cut(s) 219, 652
MwoI GCNNNNNNNGC 4 cut(s) 92, 223, 785, 833
NciI CCSGG 1 cut(s) 484
NdeII GATC 9 cut(s) 232, 396, 555, 624, 660, 697, 703, 889, 901
NlaIII CATG 3 cut(s) 116, 140, 293
NlaIV GGNNCC 5 cut(s) 273, 412, 413, 557, 770
NspI RCATGY 1 cut(s) 116
OliI CACNNNNGTG 1 cut(s) 336
PciI ACATGT 1 cut(s) 112
PciSI GCTCTTC 1 cut(s) 851
PctI GAATGC 1 cut(s) 535
PfoI TCCNGGA 1 cut(s) 650
PinAI ACCGGT 1 cut(s) 274
PkrI GCNGC 5 cut(s) 85, 430, 694, 813, 829
Ple19I CGATCG 1 cut(s) 892
PleI GAGTC 1 cut(s) 858
PpsI GAGTC 1 cut(s) 858
PpuMI RGGWCCY 1 cut(s) 769
PscI ACATGT 1 cut(s) 112
PsiI TTATAA 1 cut(s) 326
Psp5II RGGWCCY 1 cut(s) 769
Psp6I CCWGG 2 cut(s) 217, 650
PspGI CCWGG 2 cut(s) 217, 650
PspN4I GGNNCC 5 cut(s) 273, 412, 413, 557, 770
PspOMI GGGCCC 1 cut(s) 411
PspPI GGNCC 4 cut(s) 411, 412, 749, 769
PspPPI RGGWCCY 1 cut(s) 769
PstI CTGCAG 1 cut(s) 85
PsuI RGATCY 3 cut(s) 555, 624, 703
PvuI CGATCG 1 cut(s) 892
PvuII CAGCTG 2 cut(s) 86, 695
RseI CAYNNNNRTG 2 cut(s) 294, 336
SapI GCTCTTC 1 cut(s) 851
SaqAI TTAA 2 cut(s) 266, 928
SatI GCNGC 5 cut(s) 84, 429, 693, 812, 828
Sau3AI GATC 9 cut(s) 232, 396, 555, 624, 660, 697, 703, 889, 901
Sau96I GGNCC 4 cut(s) 411, 412, 749, 769
SchI GAGTC 1 cut(s) 859
ScrFI CCNGG 3 cut(s) 219, 484, 652
SduI GDGCHC 1 cut(s) 415
SfcI CTRYAG 3 cut(s) 45, 81, 831
SinI GGWCC 2 cut(s) 749, 769
SmiMI CAYNNNNRTG 2 cut(s) 294, 336
SmlI CTYRAG 1 cut(s) 613
SmoI CTYRAG 1 cut(s) 613
Sse9I AATT 4 cut(s) 105, 263, 564, 632
SsiI CCGC 2 cut(s) 811, 828
SspI AATATT 1 cut(s) 366
SspMI CTAG 2 cut(s) 92, 165
StyD4I CCNGG 3 cut(s) 217, 482, 650
TaaI ACNGT 3 cut(s) 46, 78, 674
TaiI ACGT 1 cut(s) 874
TaqI TCGA 3 cut(s) 449, 629, 784
TasI AATT 4 cut(s) 105, 263, 564, 632
TauI GCSGC 2 cut(s) 814, 830
Tru1I TTAA 2 cut(s) 266, 928
Tru9I TTAA 2 cut(s) 266, 928
TscAI CASTG 1 cut(s) 643
TseI GCWGC 3 cut(s) 83, 428, 692
TspDTI ATGAA 3 cut(s) 165, 276, 755
TspGWI ACGGA 2 cut(s) 264, 348
TspRI CASTG 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 749, 769
XapI RAATTY 1 cut(s) 632
XceI RCATGY 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 2 cut(s) 92, 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.