RchiOBHm_Chr6g0257311

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001929
Physical Location & Seq
Reverse (-)
12612124 .. 12613029
906 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23080

Sequence Viewer

Length: 720 bp
ATGAATTGGTTAAAGACCGAAATTGGGTTCAGCGGATGGCGATTTGATTTTGTCAAGGGATATGCTCCAGAGTTCACCAAGTTATATGTGGCAAACACAAAGCCTAGTTTCTCAGTAGGAGAGTTTTGGAACTCCCTTGCTTATGGAAGTGATGGTAAACCGGAGTACAACCAGGACAACCATCGTAAAGAGCTAGCTGGATGGATAGGGAATGCTGGAGGAGAGGTTACCGCATTTGATTTTACAACTAAAGGGATACTTCAAGCTGCTGTCCAAGGTGAACTGGGGAGGTTGAAGGACTCAAATGGAGGGCCTCCTGGATTGATTGGAATATCGCCACGCAATAGTGTGACTTTCATAGACAATCATGATACAGGTTCCACACAAAAGTTGTGGCCATTTCCATCAGATAAAGTCATTCAGGGATATGCTTACGTTCTTACTCATCCAGGGATCCCATCGATATTCTATGATCATTTTTTTGACTGGGGATTGAAAGAGGAGATCTCGAAACTCACTGCGATTAGGTCCAGGAATGGGATCAAACCCGACAGTGCCGTGCATATCCTAGCAGCTGATGCAGATCTCTATTTAGCTTCCATAGATGATAAAATCATAACCAAGATTGGTCCAAGATCCGACGTTGGGAACCTTGTACCTCCAACATTCAAAATTGCAACCTCTGGAAATGATTATAGTGTTTGGGAGAAAACAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

239

Amino Acids

26.31

Weight (kDa)

5.96

Isoelectric Point (pI)

27.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amyl_C2 PF07821 178 - 236 5.5e-22 Alpha-amylase C-terminal beta-sheet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 33, 231
AclWI GGATC 4 cut(s) 448, 461, 548, 630
AcoI YGGCCR 1 cut(s) 395
AfaI GTAC 2 cut(s) 167, 657
AfiI CCNNNNNNNGG 3 cut(s) 24, 537, 645
AgsI TTSAA 4 cut(s) 263, 295, 496, 670
AjnI CCWGG 4 cut(s) 171, 316, 448, 530
AluBI AGCT 5 cut(s) 193, 197, 266, 575, 596
AluI AGCT 5 cut(s) 193, 197, 266, 575, 596
AlwI GGATC 4 cut(s) 448, 461, 548, 630
AoxI GGCC 2 cut(s) 311, 395
ApeKI GCWGC 2 cut(s) 266, 572
AspS9I GGNCC 3 cut(s) 311, 528, 629
AsuHPI GGTGA 2 cut(s) 67, 290
AsuNHI GCTAGC 1 cut(s) 193
AvaII GGWCC 2 cut(s) 528, 629
BalI TGGCCA 1 cut(s) 397
BamHI GGATCC 1 cut(s) 453
BbvI GCAGC 2 cut(s) 253, 584
BccI CCATC 6 cut(s) 30, 146, 189, 195, 412, 466
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 4 cut(s) 173, 318, 450, 532
BciVI GTATCC 1 cut(s) 249
BclI TGATCA 1 cut(s) 472
BfaI CTAG 3 cut(s) 105, 194, 569
BfuI GTATCC 1 cut(s) 249
BglII AGATCT 2 cut(s) 504, 583
BisI GCNGC 2 cut(s) 267, 573
BlsI GCNGC 2 cut(s) 268, 574
Bme1390I CCNGG 4 cut(s) 173, 318, 450, 532
Bme18I GGWCC 2 cut(s) 528, 629
BmgT120I GGNCC 3 cut(s) 311, 528, 629
BmiI GGNNCC 3 cut(s) 379, 455, 650
BmrFI CCNGG 4 cut(s) 173, 318, 450, 532
BmrI ACTGGG 2 cut(s) 293, 496
BmsI GCATC 1 cut(s) 568
BmtI GCTAGC 1 cut(s) 197
BmuI ACTGGG 2 cut(s) 293, 496
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BpmI CTGGAG 2 cut(s) 51, 237
Bsa29I ATCGAT 1 cut(s) 461
BsaBI GATNNNNATC 1 cut(s) 582
BsaJI CCNNGG 2 cut(s) 274, 449
BsaWI WCCGGW 1 cut(s) 160
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 537, 645
Bse1I ACTGG 2 cut(s) 288, 491
Bse8I GATNNNNATC 1 cut(s) 582
BseBI CCWGG 4 cut(s) 173, 318, 450, 532
BseCI ATCGAT 1 cut(s) 461
BseDI CCNNGG 2 cut(s) 274, 449
BseGI GGATG 3 cut(s) 41, 206, 445
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 3 cut(s) 24, 537, 645
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 2 cut(s) 288, 491
BseRI GAGGAG 2 cut(s) 234, 515
BseXI GCAGC 2 cut(s) 253, 584
BshFI GGCC 2 cut(s) 313, 397
BshVI ATCGAT 1 cut(s) 461
BsiSI CCGG 1 cut(s) 161
BslI CCNNNNNNNGG 3 cut(s) 24, 537, 645
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 2 cut(s) 313, 397
Bsp143I GATC 6 cut(s) 453, 472, 504, 540, 583, 635
BspACI CCGC 2 cut(s) 33, 231
BspANI GGCC 2 cut(s) 313, 397
BspCNI CTCAG 1 cut(s) 125
BspDI ATCGAT 1 cut(s) 461
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 3 cut(s) 379, 455, 650
BspOI GCTAGC 1 cut(s) 197
BspPI GGATC 4 cut(s) 448, 461, 548, 630
BsrI ACTGG 2 cut(s) 288, 491
BssECI CCNNGG 2 cut(s) 274, 449
BssMI GATC 6 cut(s) 453, 472, 504, 540, 583, 635
BssT1I CCWWGG 1 cut(s) 274
Bst2UI CCWGG 4 cut(s) 173, 318, 450, 532
Bst4CI ACNGT 1 cut(s) 554
BstAPI GCANNNNNTGC 1 cut(s) 578
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 1 cut(s) 112
BstEII GGTNACC 1 cut(s) 226
BstF5I GGATG 3 cut(s) 41, 206, 445
BstKTI GATC 6 cut(s) 456, 475, 507, 543, 586, 638
BstMBI GATC 6 cut(s) 453, 472, 504, 540, 583, 635
BstMWI GCNNNNNNNGC 1 cut(s) 578
BstNI CCWGG 4 cut(s) 173, 318, 450, 532
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 4 cut(s) 171, 316, 448, 530
BstV1I GCAGC 2 cut(s) 253, 584
BstX2I RGATCY 4 cut(s) 453, 504, 583, 635
BstYI RGATCY 4 cut(s) 453, 504, 583, 635
Bsu15I ATCGAT 1 cut(s) 461
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 2 cut(s) 313, 397
BsuTUI ATCGAT 1 cut(s) 461
BtsCI GGATG 3 cut(s) 41, 206, 445
BtsI GCAGTG 1 cut(s) 516
BtsIMutI CAGTG 2 cut(s) 516, 559
Cac8I GCNNGC 1 cut(s) 195
CciI TCATGA 1 cut(s) 367
Cfr13I GGNCC 3 cut(s) 311, 528, 629
ClaI ATCGAT 1 cut(s) 461
Csp6I GTAC 2 cut(s) 166, 656
CspCI CAANNNNNGTGG 2 cut(s) 374, 409
CviAII CATG 2 cut(s) 368, 717
CviJI RGCY 8 cut(s) 103, 193, 197, 266, 313, 397, 575, 596
CviKI_1 RGCY 8 cut(s) 103, 193, 197, 266, 313, 397, 575, 596
CviQI GTAC 2 cut(s) 166, 656
DdeI CTNAG 1 cut(s) 112
DpnI GATC 6 cut(s) 455, 474, 506, 542, 585, 637
DpnII GATC 6 cut(s) 453, 472, 504, 540, 583, 635
EaeI YGGCCR 1 cut(s) 395
Eco130I CCWWGG 1 cut(s) 274
Eco47I GGWCC 2 cut(s) 528, 629
Eco91I GGTNACC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 311
EcoO65I GGTNACC 1 cut(s) 226
EcoRII CCWGG 4 cut(s) 171, 316, 448, 530
EcoT14I CCWWGG 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 274
FaeI CATG 2 cut(s) 371, 720
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FatI CATG 2 cut(s) 367, 716
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 2 cut(s) 267, 573
FokI GGATG 3 cut(s) 48, 213, 432
Fsp4HI GCNGC 2 cut(s) 267, 573
FspBI CTAG 3 cut(s) 105, 194, 569
GluI GCNGC 2 cut(s) 267, 573
GsuI CTGGAG 2 cut(s) 51, 237
HaeIII GGCC 2 cut(s) 313, 397
HapII CCGG 1 cut(s) 161
Hin1II CATG 2 cut(s) 371, 720
HinfI GANTC 1 cut(s) 299
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 67, 290
Hpy166II GTNNAC 3 cut(s) 75, 158, 281
Hpy188I TCNGA 2 cut(s) 409, 640
Hpy188III TCNNGA 4 cut(s) 68, 368, 508, 684
Hpy8I GTNNAC 3 cut(s) 75, 158, 281
Hpy99I CGWCG 1 cut(s) 644
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 1 cut(s) 554
HpyCH4IV ACGT 2 cut(s) 435, 642
HpyCH4V TGCA 3 cut(s) 562, 581, 677
HpyF10VI GCNNNNNNNGC 1 cut(s) 578
HpyF3I CTNAG 1 cut(s) 112
HpySE526I ACGT 2 cut(s) 435, 642
Hsp92II CATG 2 cut(s) 371, 720
Ksp22I TGATCA 1 cut(s) 472
Kzo9I GATC 6 cut(s) 453, 472, 504, 540, 583, 635
LmnI GCTCC 1 cut(s) 70
Lsp1109I GCAGC 2 cut(s) 253, 584
LweI GCATC 1 cut(s) 568
MaeI CTAG 3 cut(s) 105, 194, 569
MaeII ACGT 2 cut(s) 435, 642
MaeIII GTNAC 2 cut(s) 226, 349
MalI GATC 6 cut(s) 455, 474, 506, 542, 585, 637
MboI GATC 6 cut(s) 453, 472, 504, 540, 583, 635
MflI RGATCY 4 cut(s) 453, 504, 583, 635
MlsI TGGCCA 1 cut(s) 397
MluCI AATT 3 cut(s) 4, 21, 672
MluNI TGGCCA 1 cut(s) 397
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 2 cut(s) 663, 686
MnlI CCTC 8 cut(s) 212, 217, 282, 302, 324, 493, 669, 691
Mox20I TGGCCA 1 cut(s) 397
MscI TGGCCA 1 cut(s) 397
MseI TTAA 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 397
MspA1I CMGCKG 2 cut(s) 33, 575
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 4 cut(s) 173, 318, 450, 532
Mva1269I GAATGC 1 cut(s) 217
MvaI CCWGG 4 cut(s) 173, 318, 450, 532
MwoI GCNNNNNNNGC 1 cut(s) 578
NdeII GATC 6 cut(s) 453, 472, 504, 540, 583, 635
NheI GCTAGC 1 cut(s) 193
NlaIII CATG 2 cut(s) 371, 720
NlaIV GGNNCC 3 cut(s) 379, 455, 650
NmuCI GTSAC 1 cut(s) 349
PagI TCATGA 1 cut(s) 367
PctI GAATGC 1 cut(s) 217
PfoI TCCNGGA 2 cut(s) 316, 530
PkrI GCNGC 2 cut(s) 268, 574
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
Psp6I CCWGG 4 cut(s) 171, 316, 448, 530
PspEI GGTNACC 1 cut(s) 226
PspGI CCWGG 4 cut(s) 171, 316, 448, 530
PspN4I GGNNCC 3 cut(s) 379, 455, 650
PspPI GGNCC 3 cut(s) 311, 528, 629
PsuI RGATCY 4 cut(s) 453, 504, 583, 635
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 2 cut(s) 167, 657
RsaNI GTAC 2 cut(s) 166, 656
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 2 cut(s) 267, 573
Sau3AI GATC 6 cut(s) 453, 472, 504, 540, 583, 635
Sau96I GGNCC 3 cut(s) 311, 528, 629
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 4 cut(s) 173, 318, 450, 532
SfaNI GCATC 1 cut(s) 568
SinI GGWCC 2 cut(s) 528, 629
Sse9I AATT 3 cut(s) 4, 21, 672
SsiI CCGC 2 cut(s) 33, 231
SspMI CTAG 3 cut(s) 105, 194, 569
StyD4I CCNGG 4 cut(s) 171, 316, 448, 530
StyI CCWWGG 1 cut(s) 274
TaaI ACNGT 1 cut(s) 554
TaiI ACGT 2 cut(s) 438, 645
TaqI TCGA 2 cut(s) 461, 509
TaqII GACCGA 1 cut(s) 32
TasI AATT 3 cut(s) 4, 21, 672
TatI WGTACW 1 cut(s) 165
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TscAI CASTG 2 cut(s) 523, 559
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 2 cut(s) 266, 572
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 2 cut(s) 17, 346
TspRI CASTG 2 cut(s) 523, 559
VpaK11BI GGWCC 2 cut(s) 528, 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XspI CTAG 3 cut(s) 105, 194, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.