Rorug05G0573800

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001929
Physical Location & Seq
Forward (+)
76895358 .. 76898715
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0573800.1

Sequence Viewer

Length: 1593 bp
ATGGAAACAGTTGAGAACTTAAATTGCATATACAGAAACCCAAACGAACCCATTGAAGCTCGAGTCAAGGACCTCCTTTCTCGCATGACTTTGAATGAAAAGGTGGGGCAGATGACCCAGATAGAGCGTCAGGTCGCTACACCTTCTGCCATTAAAGACTTCTCAATCGGGAGTGTAACCAGTGGTGCAGGAAGTGGGCCGTTTAGGAAGGCTTTATCGGCGGACTGGGCTGATATGGTTGACGGGTTTCAGAGGTGTGCGCTGGAAACCCGGCTCCGGATTCCATTGATATGGATTGATGCTGTCCATGGGAACAATAGCGTCTACGGTGCCACTATATTTCCTCATAATGTTGGTCTTGGGGCTACCAGGGTGACCATTGGGTTGCATTTTCTCTGTTTCAATCTTTTCCTTGTAGTATATATCAAGGTTGCTTCGACGTCAAATGCGGATTTGGCTCAAAGGATTGGTGAGGCAACTGCTCTTGAAGTCAGGGCTAGTGGCATGCACTACACTTTTGCTCCCTGTGTAGCTGTATGCAGGGATCCCAGGTGGGGACGATGCTACGAGAGTTACAGCGAAGACACTGAAATTGTTAGAAAGATGACATCCATTATCTCAGGCTTGCAGGGGAAGCCACCCCAAGGGCATGAAAATGGTTACCCTTTTATAACGGGAAGAAACAACACAATTGCATGCGCCAAGCATTTTATTGGAGATGGCGGTACACATAAAGGTGTAAATGAGGGAAATACCATATCATCTTACGATGACTTAGAGAGGATTCATATGGCACCTTATCTGGACTGCATTTCTCAAGGAGTTTCCACTATTATGGCATCTTATTCCAGCTGGAATGGAAATAGACTACATGCTGATCGTTTTCTCCTGACTGAAATCTTGAAAGAAAAGCTAGGATTTAAGGGAATTGTGATTTCTGACTGGGATGGTATTGAAACACTCAGTGAGCCTCAAGGGTCAAACTATCGTTTCTGCGTTTTATCTGCTGTCAATGCTGGAATTGACATGTTTATGGTGCCTTTCAGATTTGAAAATTTTGTGGATGATTTAGTGAATCTGGTGGAACATGGGGAGATACCAATGTCCAGGATAGATGATGCTGTTGAGAGAATACTGAGAGTAAAGTTTGTTGCTGGTCTTTTTGAACATCCCTTTTCTGATAGATCTTTGCTGGACAAAGTTGGTTGTAAGCTGCATAGGGATCTGGCATGCGAAGCTGTTCGCAAGTCATTGGTTCTGTTGAAGAATGGAAAGGAGCCAAGAAAACCTTTTCTTCCATTAGATAGAAAAGCCAAAAGAATACTGGTTACCGGAATGCATGCTGATGATCTTGGATATCAGTGTGGAGGATGGACAGTTACTAAGGCTGGATTAAGTGGCAGGTTCACCATTGGCACAACCATCTTGGAAGCTATCAAGAAGGCAGTTGGAGAGGAAACAGAAATAATTTATGAGCAACTGCCATCGACGTATACCCTAGCACGTACAGATATCTCTTTTGCAGTTGTTGCTGTTGGTGAAGGTTCATATGCCGAATGCAAGGGCGACAACTTAGATTGTGATTCCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

530

Amino Acids

58.55

Weight (kDa)

6.05

Isoelectric Point (pI)

26.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 30 - 124 7.5e-09 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3 PF00933 144 - 381 4.1e-57 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 418 - 526 8.1e-09 Glycosyl hydrolase family 3 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 671
AatII GACGTC 1 cut(s) 443
Acc36I ACCTGC 1 cut(s) 1392
AccB1I GGYRCC 3 cut(s) 329, 793, 1036
AccI GTMKAC 2 cut(s) 324, 1493
AccIII TCCGGA 1 cut(s) 276
AciI CCGC 3 cut(s) 221, 449, 723
AclWI GGATC 3 cut(s) 539, 552, 1230
AcsI RAATTY 1 cut(s) 1054
AcyI GRCGYC 1 cut(s) 440
AdeI CACNNNGTG 1 cut(s) 965
AfaI GTAC 2 cut(s) 727, 1507
AfiI CCNNNNNNNGG 3 cut(s) 276, 554, 644
AflIII ACRYGT 1 cut(s) 1026
AgsI TTSAA 9 cut(s) 56, 94, 403, 488, 904, 956, 1052, 1166, 1264
AjnI CCWGG 3 cut(s) 368, 548, 1106
AluBI AGCT 7 cut(s) 59, 533, 852, 913, 1213, 1238, 1433
AluI AGCT 7 cut(s) 59, 533, 852, 913, 1213, 1238, 1433
AlwI GGATC 3 cut(s) 539, 552, 1230
Ama87I CYCGRG 1 cut(s) 60
Aor13HI TCCGGA 1 cut(s) 276
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 1 cut(s) 1213
ApoI RAATTY 1 cut(s) 1054
Asp700I GAANNNNTTC 1 cut(s) 1239
AspLEI GCGC 2 cut(s) 262, 701
AspS9I GGNCC 2 cut(s) 70, 197
AsuC2I CCSGG 1 cut(s) 271
AsuHPI GGTGA 4 cut(s) 385, 482, 1399, 1550
AvaI CYCGRG 1 cut(s) 60
AvaII GGWCC 1 cut(s) 70
BaeI ACNNNNGTAYC 2 cut(s) 1088, 1121
BamHI GGATCC 1 cut(s) 544
BanI GGYRCC 3 cut(s) 329, 793, 1036
BbsI GAAGAC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 1200
BccI CCATC 5 cut(s) 713, 941, 1365, 1430, 1492
BceAI ACGGC 1 cut(s) 184
BciT130I CCWGG 3 cut(s) 370, 550, 1108
BcnI CCSGG 1 cut(s) 271
BfaI CTAG 3 cut(s) 498, 914, 1499
BfuAI ACCTGC 1 cut(s) 1392
BglII AGATCT 1 cut(s) 1184
BisI GCNGC 1 cut(s) 1214
BlsI GCNGC 1 cut(s) 1215
Bme1390I CCNGG 4 cut(s) 271, 370, 550, 1108
Bme18I GGWCC 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 60
BmgT120I GGNCC 2 cut(s) 70, 197
BmiI GGNNCC 6 cut(s) 275, 331, 546, 795, 1038, 1278
BmrFI CCNGG 4 cut(s) 271, 370, 550, 1108
BmrI ACTGGG 2 cut(s) 235, 952
BmsI GCATC 4 cut(s) 289, 551, 848, 1108
BmuI ACTGGG 2 cut(s) 235, 952
BpiI GAAGAC 1 cut(s) 588
BpuEI CTTGAG 2 cut(s) 801, 957
BpuMI CCSGG 1 cut(s) 271
BsaAI YACGTR 1 cut(s) 1505
BsaHI GRCGYC 1 cut(s) 440
BsaJI CCNNGG 4 cut(s) 307, 369, 548, 643
BsaWI WCCGGW 2 cut(s) 276, 1331
BsaXI ACNNNNNCTCC 2 cut(s) 505, 535
Bsc4I CCNNNNNNNGG 3 cut(s) 276, 554, 644
Bse1I ACTGG 4 cut(s) 180, 230, 947, 1329
BseAI TCCGGA 1 cut(s) 276
BseBI CCWGG 3 cut(s) 370, 550, 1108
BseDI CCNNGG 4 cut(s) 307, 369, 548, 643
BseGI GGATG 5 cut(s) 608, 952, 1069, 1168, 1376
BseLI CCNNNNNNNGG 3 cut(s) 276, 554, 644
BseMII CTCAG 3 cut(s) 633, 976, 1127
BseNI ACTGG 4 cut(s) 180, 230, 947, 1329
BseXI GCAGC 1 cut(s) 1200
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 1 cut(s) 199
BshNI GGYRCC 3 cut(s) 329, 793, 1036
BsiHKCI CYCGRG 1 cut(s) 60
BsiSI CCGG 3 cut(s) 271, 277, 1332
BslFI GGGAC 1 cut(s) 570
BslI CCNNNNNNNGG 3 cut(s) 276, 554, 644
BsmFI GGGAC 1 cut(s) 570
BsmI GAATGC 2 cut(s) 1341, 1562
BsnI GGCC 1 cut(s) 199
BsoBI CYCGRG 1 cut(s) 60
Bsp13I TCCGGA 1 cut(s) 276
Bsp143I GATC 5 cut(s) 544, 877, 1184, 1222, 1348
Bsp19I CCATGG 1 cut(s) 307
BspACI CCGC 3 cut(s) 221, 449, 723
BspANI GGCC 1 cut(s) 199
BspCNI CTCAG 3 cut(s) 632, 975, 1128
BspEI TCCGGA 1 cut(s) 276
BspLI GGNNCC 6 cut(s) 275, 331, 546, 795, 1038, 1278
BspMI ACCTGC 1 cut(s) 1392
BspPI GGATC 3 cut(s) 539, 552, 1230
BspT107I GGYRCC 3 cut(s) 329, 793, 1036
BsrI ACTGG 4 cut(s) 180, 230, 947, 1329
BssECI CCNNGG 4 cut(s) 307, 369, 548, 643
BssMI GATC 5 cut(s) 544, 877, 1184, 1222, 1348
BssNAI GTATAC 1 cut(s) 1494
BssNI GRCGYC 1 cut(s) 440
BssT1I CCWWGG 2 cut(s) 307, 643
Bst1107I GTATAC 1 cut(s) 1494
Bst2UI CCWGG 3 cut(s) 370, 550, 1108
Bst4CI ACNGT 3 cut(s) 10, 329, 1378
BstACI GRCGYC 1 cut(s) 440
BstAPI GCANNNNNTGC 1 cut(s) 1529
BstBAI YACGTR 1 cut(s) 1505
BstC8I GCNNGC 5 cut(s) 506, 626, 697, 1231, 1341
BstDEI CTNAG 6 cut(s) 619, 775, 962, 1136, 1383, 1573
BstDSI CCRYGG 1 cut(s) 307
BstEII GGTNACC 3 cut(s) 373, 659, 1327
BstF5I GGATG 5 cut(s) 608, 952, 1069, 1168, 1376
BstHHI GCGC 2 cut(s) 262, 701
BstKTI GATC 5 cut(s) 547, 880, 1187, 1225, 1351
BstMBI GATC 5 cut(s) 544, 877, 1184, 1222, 1348
BstMWI GCNNNNNNNGC 7 cut(s) 218, 227, 455, 634, 1013, 1235, 1529
BstNI CCWGG 3 cut(s) 370, 550, 1108
BstNSI RCATGY 6 cut(s) 508, 699, 875, 1030, 1233, 1343
BstPI GGTNACC 3 cut(s) 373, 659, 1327
BstSCI CCNGG 4 cut(s) 269, 368, 548, 1106
BstV1I GCAGC 1 cut(s) 1200
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 3 cut(s) 544, 1184, 1222
BstXI CCANNNNNNTGG 2 cut(s) 291, 835
BstYI RGATCY 3 cut(s) 544, 1184, 1222
BstZ17I GTATAC 1 cut(s) 1494
BsuRI GGCC 1 cut(s) 199
BtgI CCRYGG 1 cut(s) 307
BtsCI GGATG 5 cut(s) 608, 952, 1069, 1168, 1376
BtsIMutI CAGTG 4 cut(s) 187, 585, 970, 1367
BveI ACCTGC 1 cut(s) 1392
Cac8I GCNNGC 5 cut(s) 506, 626, 697, 1231, 1341
CfoI GCGC 2 cut(s) 262, 701
Cfr13I GGNCC 2 cut(s) 70, 197
CseI GACGC 2 cut(s) 116, 310
Csp6I GTAC 2 cut(s) 726, 1506
CviQI GTAC 2 cut(s) 726, 1506
DdeI CTNAG 6 cut(s) 619, 775, 962, 1136, 1383, 1573
DpnI GATC 5 cut(s) 546, 879, 1186, 1224, 1350
DpnII GATC 5 cut(s) 544, 877, 1184, 1222, 1348
DraIII CACNNNGTG 1 cut(s) 965
EciI GGCGGA 1 cut(s) 236
Eco130I CCWWGG 2 cut(s) 307, 643
Eco32I GATATC 2 cut(s) 1358, 1513
Eco47I GGWCC 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 60
Eco91I GGTNACC 3 cut(s) 373, 659, 1327
EcoO109I RGGNCCY 1 cut(s) 70
EcoO65I GGTNACC 3 cut(s) 373, 659, 1327
EcoRII CCWGG 3 cut(s) 368, 548, 1106
EcoRV GATATC 2 cut(s) 1358, 1513
EcoT14I CCWWGG 2 cut(s) 307, 643
EcoT22I ATGCAT 1 cut(s) 1341
ErhI CCWWGG 2 cut(s) 307, 643
FalI AAGNNNNNCTT 2 cut(s) 1273, 1305
FaqI GGGAC 1 cut(s) 570
FauNDI CATATG 2 cut(s) 789, 1549
FblI GTMKAC 2 cut(s) 324, 1493
Fnu4HI GCNGC 1 cut(s) 1214
FokI GGATG 5 cut(s) 595, 959, 1076, 1155, 1383
Fsp4HI GCNGC 1 cut(s) 1214
FspBI CTAG 3 cut(s) 498, 914, 1499
GlaI GCGC 2 cut(s) 261, 700
GluI GCNGC 1 cut(s) 1214
HaeIII GGCC 1 cut(s) 199
HapII CCGG 3 cut(s) 271, 277, 1332
HgaI GACGC 2 cut(s) 116, 310
HhaI GCGC 2 cut(s) 262, 701
Hin1I GRCGYC 1 cut(s) 440
Hin6I GCGC 2 cut(s) 260, 699
HinP1I GCGC 2 cut(s) 260, 699
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 5 cut(s) 63, 280, 784, 1075, 1583
HpaII CCGG 3 cut(s) 271, 277, 1332
HphI GGTGA 4 cut(s) 385, 482, 1399, 1550
Hpy166II GTNNAC 5 cut(s) 241, 325, 728, 1407, 1494
Hpy188I TCNGA 4 cut(s) 252, 940, 1046, 1180
Hpy188III TCNNGA 7 cut(s) 169, 277, 485, 803, 889, 901, 1438
Hpy8I GTNNAC 5 cut(s) 241, 325, 728, 1407, 1494
Hpy99I CGWCG 2 cut(s) 442, 1492
HpyAV CCTTC 4 cut(s) 153, 202, 1435, 1535
HpyCH4III ACNGT 3 cut(s) 10, 329, 1378
HpyCH4IV ACGT 3 cut(s) 440, 1490, 1504
HpyF10VI GCNNNNNNNGC 7 cut(s) 218, 227, 455, 634, 1013, 1235, 1529
HpyF3I CTNAG 6 cut(s) 619, 775, 962, 1136, 1383, 1573
HpySE526I ACGT 3 cut(s) 440, 1490, 1504
Hsp92I GRCGYC 1 cut(s) 440
HspAI GCGC 2 cut(s) 260, 699
Kpn2I TCCGGA 1 cut(s) 276
Kzo9I GATC 5 cut(s) 544, 877, 1184, 1222, 1348
LmnI GCTCC 3 cut(s) 279, 526, 1276
Lsp1109I GCAGC 1 cut(s) 1200
LweI GCATC 4 cut(s) 289, 551, 848, 1108
MaeI CTAG 3 cut(s) 498, 914, 1499
MaeII ACGT 3 cut(s) 440, 1490, 1504
MaeIII GTNAC 6 cut(s) 175, 373, 572, 659, 1327, 1378
MalI GATC 5 cut(s) 546, 879, 1186, 1224, 1350
MboI GATC 5 cut(s) 544, 877, 1184, 1222, 1348
MboII GAAGA 4 cut(s) 593, 690, 1276, 1286
MfeI CAATTG 1 cut(s) 690
MflI RGATCY 3 cut(s) 544, 1184, 1222
MluCI AATT 7 cut(s) 22, 591, 690, 927, 1020, 1054, 1467
MlyI GAGTC 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 1429
MnlI CCTC 9 cut(s) 83, 246, 354, 466, 739, 774, 981, 1361, 1447
Mph1103I ATGCAT 1 cut(s) 1341
MroI TCCGGA 1 cut(s) 276
MroXI GAANNNNTTC 1 cut(s) 1239
MseI TTAA 4 cut(s) 20, 153, 921, 1394
MslI CAYNNNNRTG 6 cut(s) 289, 654, 735, 833, 1031, 1344
MspA1I CMGCKG 1 cut(s) 852
MspI CCGG 3 cut(s) 271, 277, 1332
MspR9I CCNGG 4 cut(s) 271, 370, 550, 1108
MunI CAATTG 1 cut(s) 690
Mva1269I GAATGC 2 cut(s) 1341, 1562
MvaI CCWGG 3 cut(s) 370, 550, 1108
MwoI GCNNNNNNNGC 7 cut(s) 218, 227, 455, 634, 1013, 1235, 1529
NciI CCSGG 1 cut(s) 271
NcoI CCATGG 1 cut(s) 307
NdeI CATATG 2 cut(s) 789, 1549
NdeII GATC 5 cut(s) 544, 877, 1184, 1222, 1348
NlaIV GGNNCC 6 cut(s) 275, 331, 546, 795, 1038, 1278
NmuCI GTSAC 1 cut(s) 373
NsiI ATGCAT 1 cut(s) 1341
NspI RCATGY 6 cut(s) 508, 699, 875, 1030, 1233, 1343
PaeI GCATGC 4 cut(s) 508, 699, 1233, 1343
PaeR7I CTCGAG 1 cut(s) 60
PciI ACATGT 1 cut(s) 1026
PctI GAATGC 2 cut(s) 1341, 1562
PdmI GAANNNNTTC 1 cut(s) 1239
PfeI GAWTC 4 cut(s) 280, 784, 1075, 1583
PfoI TCCNGGA 1 cut(s) 1106
PkrI GCNGC 1 cut(s) 1215
PleI GAGTC 1 cut(s) 71
PpsI GAGTC 1 cut(s) 71
Ppu21I YACGTR 1 cut(s) 1505
PpuMI RGGWCCY 1 cut(s) 70
PscI ACATGT 1 cut(s) 1026
PsiI TTATAA 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 70
Psp6I CCWGG 3 cut(s) 368, 548, 1106
PspEI GGTNACC 3 cut(s) 373, 659, 1327
PspGI CCWGG 3 cut(s) 368, 548, 1106
PspN4I GGNNCC 6 cut(s) 275, 331, 546, 795, 1038, 1278
PspPI GGNCC 2 cut(s) 70, 197
PspPPI RGGWCCY 1 cut(s) 70
PspXI VCTCGAGB 1 cut(s) 60
PsuI RGATCY 3 cut(s) 544, 1184, 1222
PvuII CAGCTG 1 cut(s) 852
RsaI GTAC 2 cut(s) 727, 1507
RsaNI GTAC 2 cut(s) 726, 1506
RseI CAYNNNNRTG 6 cut(s) 289, 654, 735, 833, 1031, 1344
SaqAI TTAA 4 cut(s) 20, 153, 921, 1394
SatI GCNGC 1 cut(s) 1214
Sau3AI GATC 5 cut(s) 544, 877, 1184, 1222, 1348
Sau96I GGNCC 2 cut(s) 70, 197
SchI GAGTC 1 cut(s) 72
ScrFI CCNGG 4 cut(s) 271, 370, 550, 1108
SfaNI GCATC 4 cut(s) 289, 551, 848, 1108
Sfr274I CTCGAG 1 cut(s) 60
SinI GGWCC 1 cut(s) 70
SlaI CTCGAG 1 cut(s) 60
SmiMI CAYNNNNRTG 6 cut(s) 289, 654, 735, 833, 1031, 1344
SmlI CTYRAG 3 cut(s) 60, 816, 972
SmoI CTYRAG 3 cut(s) 60, 816, 972
SphI GCATGC 4 cut(s) 508, 699, 1233, 1343
Sse9I AATT 7 cut(s) 22, 591, 690, 927, 1020, 1054, 1467
SsiI CCGC 3 cut(s) 221, 449, 723
SspMI CTAG 3 cut(s) 498, 914, 1499
StyD4I CCNGG 4 cut(s) 269, 368, 548, 1106
StyI CCWWGG 2 cut(s) 307, 643
TaaI ACNGT 3 cut(s) 10, 329, 1378
TaiI ACGT 3 cut(s) 443, 1493, 1507
TaqI TCGA 3 cut(s) 61, 437, 1487
TasI AATT 7 cut(s) 22, 591, 690, 927, 1020, 1054, 1467
TfiI GAWTC 4 cut(s) 280, 784, 1075, 1583
Tru1I TTAA 4 cut(s) 20, 153, 921, 1394
Tru9I TTAA 4 cut(s) 20, 153, 921, 1394
TscAI CASTG 4 cut(s) 187, 592, 970, 1367
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 1 cut(s) 1213
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 4 cut(s) 111, 666, 776, 1536
TspRI CASTG 4 cut(s) 187, 592, 970, 1367
VpaK11BI GGWCC 1 cut(s) 70
XapI RAATTY 1 cut(s) 1054
XceI RCATGY 6 cut(s) 508, 699, 875, 1030, 1233, 1343
XcmI CCANNNNNNNNNTGG 1 cut(s) 1321
XhoI CTCGAG 1 cut(s) 60
XmiI GTMKAC 2 cut(s) 324, 1493
XmnI GAANNNNTTC 1 cut(s) 1239
XspI CTAG 3 cut(s) 498, 914, 1499
ZraI GACGTC 1 cut(s) 441
Zsp2I ATGCAT 1 cut(s) 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.