Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001929

Physical Location & Seq

Genomic Coordinates Forward (+)

13317560 .. 13321310

Gene Structure 3751 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G007930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1278 bp

ATGAATTCACTAAAAATTTCCCTCCTCTGTTTCCTACTCTCTATCATTTTCATACCCAACTTCACTGCTTCCCAAATCCTATTTCAGGGGTTCAATTGGGAATCGGCAAAGAAGGGGGGATGGTACAACATCCTGAAACAGTCTGCAGCTGAACTAGCTTCTTCAGGAATTACACATGTGTGGCTTCCACCACCTTCTCATGCTATTTCAGATGAAGGATACATGCCTGGCAGGCTGTATGATCTCAATGTATCTAAATATGGAAATCAAGATGATTTAAAAGCACTGATAAAAGCCTACAGTGACAGTGGGATACAGAGCATAGCAGATATTGTTATAAACCACAGATGTGCAGAGAAGCAAGATGAGAGAGGAATATGGTGCATTTTTGAAGGAGGAACACCTGATGATCGCCTTGATTGGGGGCCGTCTCTTATTTGCAGTGATGACACACAATATTCTGATGGCAAGGGCAATCCTGACACTGGAGCTGACTTTGGGGGTGCTCCTGACATTGATCACAAAAACACAAGGGTCCAGAATGAGCTCTCGGATTGGATGAATTGGTTAAAGACCGAAATTGGGTTCAGCGGATGGCGATTTGATTTTGTCAAGGGATATGCTCCAGAGTTCACCAAGTTATATGTGGCAAACACAAAGCCTAGTTTCTCAGTAGGAGAGTTTTGGAACTCCCTTGCTTATGGAAGTGATGGTAAACCGGAGTACAACCAGGACAACCATCGTAAAGAGCTTGCTGGATGGATAGGGAATGCTGGAGGAGAGGTTACCGCATTTGATTTTACAACTAAAGGGATACTTCAAGCTGCTGTCCAAGGTGAACTGGGGAGGTTGAAGGACTCAAATGGAGGGCCTCCTGGATTGATTGGAATATCGCCACGCAATAGTGTGACTTTCATAGACAATCATGATACAGGTTCCACACAAAAGTTGTGGCCATTTCCATCAGATAAAGTCATTCAGGGATATGCTTACATTCTTACTCATCCAGGGATCCCATCGATATTCTATGATCATTTCTTTGACTGGGGATTGAAAGAGGAGATCTCGAAACTCACTGCGCTTAGGTCCAGGAATGGGATCAAACCCGACAGTGCCGTGCATATCCTAGCAGCTGATGCAGATCTCTATTTAGCTTCCATAGATGATAAAATCATAACCAAGATTGGTCCAAGATCCGACGTTGGGAACCTTGTACCTCCAACATTCAAAATTGCAACCTCTGGAAATGATTATAGTGTTTGGGAGAAAACAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

425

Amino Acids

46.88

Weight (kDa)

5.17

Isoelectric Point (pI)

28.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	54 - 120	1.5e-06	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	364 - 422	1.4e-21	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001929)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25000
fragaria_vesca	FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030
malus_domestica	MD00G1035200.v1.1 MD05G1081100.v1.1 MD05G1081200.v1.1
prunus_persica	Prupe.8G125500_v2.0.a1
pyrus_communis	pycom05g07460 pycom10g07850
rosa_chinensis	RchiOBHm_Chr6g0257251 RchiOBHm_Chr6g0257281 RchiOBHm_Chr6g0257311
rosa_laevigata	RLG00000014683 RLG00000014684 RLG00000014685 RLG00000014687
rosa_multiflora	Rmu_sc0000820.1_g000005 Rmu_sc0002648.1_g000009 Rmu_sc0008660.1_g000002
rosa_roxburghii	Rroxscaffold_7G00208570 Rroxscaffold_7G00208600 Rroxscaffold_7G00208610
rosa_rugosa	Rorug04G0263800 Rorug05G0573700 Rorug05G0573800 Rorug05G0574000
rosa_samantha	Rh6AG090900 Rh6AG091400 Rh6AG091700 Rh6BG082600 Rh6BG082700 Rh6CG078700 Rh6CG079000 Rh6CG079200 Rh6DG074500 Rh6DG074800
rosa_wichuraiana	Rw6G007930 Rw6G007940 Rw6G007950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	338
AciI	CCGC	2 cut(s)	591, 789
AclWI	GGATC	4 cut(s)	1006, 1019, 1106, 1188
AcoI	YGGCCR	1 cut(s)	953
AcsI	RAATTY	2 cut(s)	4, 15
AcuI	CTGAAG	1 cut(s)	147
AfaI	GTAC	3 cut(s)	125, 725, 1215
AfiI	CCNNNNNNNGG	6 cut(s)	85, 421, 485, 582, 1095, 1203
AflIII	ACRYGT	1 cut(s)	175
AgsI	TTSAA	6 cut(s)	94, 392, 821, 853, 1054, 1228
AjnI	CCWGG	5 cut(s)	226, 729, 874, 1006, 1088
AjuI	GAANNNNNNNTTGG	2 cut(s)	66, 98
AleI	CACNNNNGTG	2 cut(s)	178, 348
AluBI	AGCT	8 cut(s)	149, 158, 491, 547, 751, 824, 1133, 1154
AluI	AGCT	8 cut(s)	149, 158, 491, 547, 751, 824, 1133, 1154
Alw21I	GWGCWC	2 cut(s)	508, 549
Alw26I	GTCTC	1 cut(s)	435
AlwI	GGATC	4 cut(s)	1006, 1019, 1106, 1188
AoxI	GGCC	3 cut(s)	425, 869, 953
ApeKI	GCWGC	3 cut(s)	146, 824, 1130
ApoI	RAATTY	2 cut(s)	4, 15
AspLEI	GCGC	1 cut(s)	1081
AspS9I	GGNCC	5 cut(s)	425, 535, 869, 1086, 1187
AsuHPI	GGTGA	2 cut(s)	625, 848
AvaII	GGWCC	3 cut(s)	535, 1086, 1187
BalI	TGGCCA	1 cut(s)	955
BamHI	GGATCC	1 cut(s)	1011
BanII	GRGCYC	1 cut(s)	549
Bbv12I	GWGCWC	2 cut(s)	508, 549
BbvI	GCAGC	3 cut(s)	158, 811, 1142
BccI	CCATC	8 cut(s)	114, 458, 588, 704, 747, 753, 970, 1024
BceAI	ACGGC	2 cut(s)	412, 1100
BciT130I	CCWGG	5 cut(s)	228, 731, 876, 1008, 1090
BciVI	GTATCC	3 cut(s)	212, 306, 807
BclI	TGATCA	2 cut(s)	517, 1030
BcoDI	GTCTC	1 cut(s)	435
BfaI	CTAG	3 cut(s)	155, 663, 1127
BfmI	CTRYAG	2 cut(s)	144, 298
BfuI	GTATCC	3 cut(s)	212, 306, 807
BglI	GCCNNNNNGGC	1 cut(s)	232
BglII	AGATCT	2 cut(s)	1062, 1141
BisI	GCNGC	3 cut(s)	147, 825, 1131
BlsI	GCNGC	3 cut(s)	148, 826, 1132
Bme1390I	CCNGG	5 cut(s)	228, 731, 876, 1008, 1090
Bme18I	GGWCC	3 cut(s)	535, 1086, 1187
BmgT120I	GGNCC	5 cut(s)	425, 535, 869, 1086, 1187
BmiI	GGNNCC	5 cut(s)	426, 536, 937, 1013, 1208
BmrFI	CCNGG	5 cut(s)	228, 731, 876, 1008, 1090
BmrI	ACTGGG	2 cut(s)	851, 1054
BmsI	GCATC	1 cut(s)	1126
BmuI	ACTGGG	2 cut(s)	851, 1054
BplI	GAGNNNNNCTC	2 cut(s)	1049, 1081
BpmI	CTGGAG	3 cut(s)	507, 609, 795
Bpu10I	CCTNAGC	1 cut(s)	1082
Bsa29I	ATCGAT	1 cut(s)	1019
BsaBI	GATNNNNATC	1 cut(s)	1140
BsaJI	CCNNGG	2 cut(s)	832, 1007
BsaWI	WCCGGW	1 cut(s)	718
BsaXI	ACNNNNNCTCC	2 cut(s)	768, 798
Bsc4I	CCNNNNNNNGG	6 cut(s)	85, 421, 485, 582, 1095, 1203
Bse1I	ACTGG	3 cut(s)	490, 846, 1049
Bse8I	GATNNNNATC	1 cut(s)	1140
BseBI	CCWGG	5 cut(s)	228, 731, 876, 1008, 1090
BseCI	ATCGAT	1 cut(s)	1019
BseDI	CCNNGG	2 cut(s)	832, 1007
BseGI	GGATG	6 cut(s)	125, 129, 564, 599, 764, 1003
BseJI	GATNNNNATC	1 cut(s)	1140
BseLI	CCNNNNNNNGG	6 cut(s)	85, 421, 485, 582, 1095, 1203
BseMII	CTCAG	1 cut(s)	684
BseNI	ACTGG	3 cut(s)	490, 846, 1049
BseRI	GAGGAG	3 cut(s)	14, 792, 1073
BseXI	GCAGC	3 cut(s)	158, 811, 1142
BsgI	GTGCAG	1 cut(s)	372
BshFI	GGCC	3 cut(s)	427, 871, 955
BshVI	ATCGAT	1 cut(s)	1019
BsiHKAI	GWGCWC	2 cut(s)	508, 549
BsiSI	CCGG	1 cut(s)	719
BslI	CCNNNNNNNGG	6 cut(s)	85, 421, 485, 582, 1095, 1203
BsmAI	GTCTC	1 cut(s)	435
BsmBI	CGTCTC	1 cut(s)	435
BsmI	GAATGC	1 cut(s)	775
BsnI	GGCC	3 cut(s)	427, 871, 955
Bsp1286I	GDGCHC	2 cut(s)	508, 549
Bsp143I	GATC	9 cut(s)	241, 409, 517, 1011, 1030, 1062, 1098, 1141, 1193
BspACI	CCGC	2 cut(s)	591, 789
BspANI	GGCC	3 cut(s)	427, 871, 955
BspCNI	CTCAG	1 cut(s)	683
BspDI	ATCGAT	1 cut(s)	1019
BspHI	TCATGA	1 cut(s)	925
BspLI	GGNNCC	5 cut(s)	426, 536, 937, 1013, 1208
BspMAI	CTGCAG	1 cut(s)	148
BspPI	GGATC	4 cut(s)	1006, 1019, 1106, 1188
BsrI	ACTGG	3 cut(s)	490, 846, 1049
BssECI	CCNNGG	2 cut(s)	832, 1007
BssMI	GATC	9 cut(s)	241, 409, 517, 1011, 1030, 1062, 1098, 1141, 1193
BssT1I	CCWWGG	1 cut(s)	832
Bst2UI	CCWGG	5 cut(s)	228, 731, 876, 1008, 1090
Bst4CI	ACNGT	4 cut(s)	141, 302, 308, 1112
BstAPI	GCANNNNNTGC	1 cut(s)	1136
BstC8I	GCNNGC	2 cut(s)	233, 753
BstDEI	CTNAG	2 cut(s)	670, 1082
BstEII	GGTNACC	1 cut(s)	784
BstENI	CCTNNNNNAGG	1 cut(s)	83
BstF5I	GGATG	6 cut(s)	125, 129, 564, 599, 764, 1003
BstHHI	GCGC	1 cut(s)	1081
BstKTI	GATC	9 cut(s)	244, 412, 520, 1014, 1033, 1065, 1101, 1144, 1196
BstMAI	GTCTC	1 cut(s)	435
BstMBI	GATC	9 cut(s)	241, 409, 517, 1011, 1030, 1062, 1098, 1141, 1193
BstMWI	GCNNNNNNNGC	3 cut(s)	155, 232, 1136
BstNI	CCWGG	5 cut(s)	228, 731, 876, 1008, 1090
BstNSI	RCATGY	2 cut(s)	179, 226
BstPI	GGTNACC	1 cut(s)	784
BstSCI	CCNGG	5 cut(s)	226, 729, 874, 1006, 1088
BstSFI	CTRYAG	2 cut(s)	144, 298
BstV1I	GCAGC	3 cut(s)	158, 811, 1142
BstX2I	RGATCY	4 cut(s)	1011, 1062, 1141, 1193
BstYI	RGATCY	4 cut(s)	1011, 1062, 1141, 1193
Bsu15I	ATCGAT	1 cut(s)	1019
BsuI	GTATCC	3 cut(s)	212, 306, 807
BsuRI	GGCC	3 cut(s)	427, 871, 955
BsuTUI	ATCGAT	1 cut(s)	1019
BtsCI	GGATG	6 cut(s)	125, 129, 564, 599, 764, 1003
BtsI	GCAGTG	3 cut(s)	63, 448, 1074
BtsIMutI	CAGTG	8 cut(s)	63, 284, 307, 313, 448, 483, 1074, 1117
Cac8I	GCNNGC	2 cut(s)	233, 753
CciI	TCATGA	1 cut(s)	925
CfoI	GCGC	1 cut(s)	1081
Cfr13I	GGNCC	5 cut(s)	425, 535, 869, 1086, 1187
ClaI	ATCGAT	1 cut(s)	1019
Csp6I	GTAC	3 cut(s)	124, 724, 1214
CspCI	CAANNNNNGTGG	2 cut(s)	932, 967
CviAII	CATG	5 cut(s)	176, 200, 223, 926, 1275
CviQI	GTAC	3 cut(s)	124, 724, 1214
DdeI	CTNAG	2 cut(s)	670, 1082
DpnI	GATC	9 cut(s)	243, 411, 519, 1013, 1032, 1064, 1100, 1143, 1195
DpnII	GATC	9 cut(s)	241, 409, 517, 1011, 1030, 1062, 1098, 1141, 1193
DraI	TTTAAA	1 cut(s)	279
EaeI	YGGCCR	1 cut(s)	953
Ecl136II	GAGCTC	1 cut(s)	547
Eco130I	CCWWGG	1 cut(s)	832
Eco24I	GRGCYC	1 cut(s)	549
Eco47I	GGWCC	3 cut(s)	535, 1086, 1187
Eco53kI	GAGCTC	1 cut(s)	547
Eco57I	CTGAAG	1 cut(s)	147
Eco91I	GGTNACC	1 cut(s)	784
EcoICRI	GAGCTC	1 cut(s)	547
EcoNI	CCTNNNNNAGG	1 cut(s)	83
EcoO109I	RGGNCCY	1 cut(s)	869
EcoO65I	GGTNACC	1 cut(s)	784
EcoRI	GAATTC	1 cut(s)	4
EcoRII	CCWGG	5 cut(s)	226, 729, 874, 1006, 1088
EcoT14I	CCWWGG	1 cut(s)	832
EcoT38I	GRGCYC	1 cut(s)	549
ErhI	CCWWGG	1 cut(s)	832
Esp3I	CGTCTC	1 cut(s)	435
FaeI	CATG	5 cut(s)	179, 203, 226, 929, 1278
FalI	AAGNNNNNCTT	2 cut(s)	801, 833
FatI	CATG	5 cut(s)	175, 199, 222, 925, 1274
FbaI	TGATCA	2 cut(s)	517, 1030
Fnu4HI	GCNGC	3 cut(s)	147, 825, 1131
FokI	GGATG	6 cut(s)	116, 132, 571, 606, 771, 990
FriOI	GRGCYC	1 cut(s)	549
Fsp4HI	GCNGC	3 cut(s)	147, 825, 1131
FspBI	CTAG	3 cut(s)	155, 663, 1127
GlaI	GCGC	1 cut(s)	1080
GluI	GCNGC	3 cut(s)	147, 825, 1131
GsuI	CTGGAG	3 cut(s)	507, 609, 795
HaeIII	GGCC	3 cut(s)	427, 871, 955
HapII	CCGG	1 cut(s)	719
HhaI	GCGC	1 cut(s)	1081
Hin1II	CATG	5 cut(s)	179, 203, 226, 929, 1278
Hin6I	GCGC	1 cut(s)	1079
HinP1I	GCGC	1 cut(s)	1079
HinfI	GANTC	2 cut(s)	101, 857
HpaII	CCGG	1 cut(s)	719
HphI	GGTGA	2 cut(s)	625, 848
Hpy166II	GTNNAC	3 cut(s)	633, 716, 839
Hpy188I	TCNGA	5 cut(s)	211, 463, 553, 967, 1198
Hpy8I	GTNNAC	3 cut(s)	633, 716, 839
Hpy99I	CGWCG	1 cut(s)	1202
HpyAV	CCTTC	5 cut(s)	106, 204, 209, 386, 847
HpyCH4III	ACNGT	4 cut(s)	141, 302, 308, 1112
HpyCH4IV	ACGT	1 cut(s)	1200
HpyCH4V	TGCA	7 cut(s)	146, 353, 384, 441, 1120, 1139, 1235
HpyF10VI	GCNNNNNNNGC	3 cut(s)	155, 232, 1136
HpyF3I	CTNAG	2 cut(s)	670, 1082
HpySE526I	ACGT	1 cut(s)	1200
Hsp92II	CATG	5 cut(s)	179, 203, 226, 929, 1278
HspAI	GCGC	1 cut(s)	1079
Ksp22I	TGATCA	2 cut(s)	517, 1030
Kzo9I	GATC	9 cut(s)	241, 409, 517, 1011, 1030, 1062, 1098, 1141, 1193
LmnI	GCTCC	3 cut(s)	488, 511, 628
Lsp1109I	GCAGC	3 cut(s)	158, 811, 1142
LweI	GCATC	1 cut(s)	1126
MaeI	CTAG	3 cut(s)	155, 663, 1127
MaeII	ACGT	1 cut(s)	1200
MaeIII	GTNAC	3 cut(s)	302, 784, 907
MalI	GATC	9 cut(s)	243, 411, 519, 1013, 1032, 1064, 1100, 1143, 1195
MboI	GATC	9 cut(s)	241, 409, 517, 1011, 1030, 1062, 1098, 1141, 1193
MboII	GAAGA	1 cut(s)	153
MfeI	CAATTG	1 cut(s)	94
MflI	RGATCY	4 cut(s)	1011, 1062, 1141, 1193
MhlI	GDGCHC	2 cut(s)	508, 549
MlsI	TGGCCA	1 cut(s)	955
MluCI	AATT	7 cut(s)	4, 15, 94, 168, 562, 579, 1230
MluNI	TGGCCA	1 cut(s)	955
MlyI	GAGTC	1 cut(s)	851
MmeI	TCCRAC	2 cut(s)	1221, 1244
Mox20I	TGGCCA	1 cut(s)	955
MscI	TGGCCA	1 cut(s)	955
MseI	TTAA	2 cut(s)	278, 569
MslI	CAYNNNNRTG	2 cut(s)	178, 348
Msp20I	TGGCCA	1 cut(s)	955
MspA1I	CMGCKG	3 cut(s)	149, 591, 1133
MspI	CCGG	1 cut(s)	719
MspR9I	CCNGG	5 cut(s)	228, 731, 876, 1008, 1090
MunI	CAATTG	1 cut(s)	94
Mva1269I	GAATGC	1 cut(s)	775
MvaI	CCWGG	5 cut(s)	228, 731, 876, 1008, 1090
MwoI	GCNNNNNNNGC	3 cut(s)	155, 232, 1136
NdeII	GATC	9 cut(s)	241, 409, 517, 1011, 1030, 1062, 1098, 1141, 1193
NlaIII	CATG	5 cut(s)	179, 203, 226, 929, 1278
NlaIV	GGNNCC	5 cut(s)	426, 536, 937, 1013, 1208
NmuCI	GTSAC	2 cut(s)	302, 907
NspI	RCATGY	2 cut(s)	179, 226
OliI	CACNNNNGTG	2 cut(s)	178, 348
PagI	TCATGA	1 cut(s)	925
PciI	ACATGT	1 cut(s)	175
PctI	GAATGC	1 cut(s)	775
PfeI	GAWTC	1 cut(s)	101
PfoI	TCCNGGA	2 cut(s)	874, 1088
PkrI	GCNGC	3 cut(s)	148, 826, 1132
PleI	GAGTC	1 cut(s)	851
PpsI	GAGTC	1 cut(s)	851
PscI	ACATGT	1 cut(s)	175
PsiI	TTATAA	1 cut(s)	338
Psp124BI	GAGCTC	1 cut(s)	549
Psp6I	CCWGG	5 cut(s)	226, 729, 874, 1006, 1088
PspEI	GGTNACC	1 cut(s)	784
PspGI	CCWGG	5 cut(s)	226, 729, 874, 1006, 1088
PspN4I	GGNNCC	5 cut(s)	426, 536, 937, 1013, 1208
PspPI	GGNCC	5 cut(s)	425, 535, 869, 1086, 1187
PstI	CTGCAG	1 cut(s)	148
PsuI	RGATCY	4 cut(s)	1011, 1062, 1141, 1193
PvuII	CAGCTG	2 cut(s)	149, 1133
RsaI	GTAC	3 cut(s)	125, 725, 1215
RsaNI	GTAC	3 cut(s)	124, 724, 1214
RseI	CAYNNNNRTG	2 cut(s)	178, 348
SacI	GAGCTC	1 cut(s)	549
SaqAI	TTAA	2 cut(s)	278, 569
SatI	GCNGC	3 cut(s)	147, 825, 1131
Sau3AI	GATC	9 cut(s)	241, 409, 517, 1011, 1030, 1062, 1098, 1141, 1193
Sau96I	GGNCC	5 cut(s)	425, 535, 869, 1086, 1187
SchI	GAGTC	1 cut(s)	851
ScrFI	CCNGG	5 cut(s)	228, 731, 876, 1008, 1090
SduI	GDGCHC	2 cut(s)	508, 549
SfaNI	GCATC	1 cut(s)	1126
SfcI	CTRYAG	2 cut(s)	144, 298
SinI	GGWCC	3 cut(s)	535, 1086, 1187
SmiMI	CAYNNNNRTG	2 cut(s)	178, 348
Sse9I	AATT	7 cut(s)	4, 15, 94, 168, 562, 579, 1230
SsiI	CCGC	2 cut(s)	591, 789
SspI	AATATT	1 cut(s)	458
SspMI	CTAG	3 cut(s)	155, 663, 1127
SstI	GAGCTC	1 cut(s)	549
StyD4I	CCNGG	5 cut(s)	226, 729, 874, 1006, 1088
StyI	CCWWGG	1 cut(s)	832
TaaI	ACNGT	4 cut(s)	141, 302, 308, 1112
TaiI	ACGT	1 cut(s)	1203
TaqI	TCGA	2 cut(s)	1019, 1067
TaqII	GACCGA	1 cut(s)	590
TasI	AATT	7 cut(s)	4, 15, 94, 168, 562, 579, 1230
TatI	WGTACW	1 cut(s)	723
TfiI	GAWTC	1 cut(s)	101
Tru1I	TTAA	2 cut(s)	278, 569
Tru9I	TTAA	2 cut(s)	278, 569
TscAI	CASTG	8 cut(s)	70, 291, 307, 313, 448, 490, 1081, 1117
TseFI	GTSAC	2 cut(s)	302, 907
TseI	GCWGC	3 cut(s)	146, 824, 1130
Tsp45I	GTSAC	2 cut(s)	302, 907
TspDTI	ATGAA	5 cut(s)	17, 40, 228, 575, 904
TspRI	CASTG	8 cut(s)	70, 291, 307, 313, 448, 490, 1081, 1117
VpaK11BI	GGWCC	3 cut(s)	535, 1086, 1187
XagI	CCTNNNNNAGG	1 cut(s)	83
XapI	RAATTY	2 cut(s)	4, 15
XceI	RCATGY	2 cut(s)	179, 226
XcmI	CCANNNNNNNNNTGG	1 cut(s)	643
XspI	CTAG	3 cut(s)	155, 663, 1127

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.