Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001929

Physical Location & Seq

Genomic Coordinates Reverse (-)

16942735 .. 16945340

Gene Structure 2606 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1081200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1299 bp

ATGAAAGCTCGAACCACTTCCCTCTTCTTTCTCCTTTTCTGTATACCTCTACTCCCCAACTTAACTGCTCCCCAGATCCTATTTCAGGGGTTCAATTGGGAATCATGGAAGGAGGAAGGAGGATGGTACAAATCTTTAAGCCAGTCCATACCTCAACAACTAGCTTCTTCTGGGATTACGCATGTTTGGCTTCCTCCACCTTCTCATTCTGTTTCACCTCAAGGATACATGCCTGGAAGGCTCTATGATCTGAATGCATCAAGATACGGAAACCAAGATGAACTCAAGGCGCTGATAAACACCTTCCATGACAACGGGATACAAAGCATTGCAGATATAGTCATAAACCACAGATGTGCAGAGAAGAAAGATGAAAGAGGAATATGGTGCATCTTCGAAGGAGGAACTCCAGATGATCGCCTTGATTGGGGCCCGTCTCTTATTTGCAGCGATGACACAGCTTATTCCAATGGCAAGGGCAACCCTGACACTGGAGCTGACTTCAGTGCTGCTCCTGACATTGATCACATTAACACAAGAGTCCAGAGAGAGCTCTCAGATTGGATGAATTGGCTAAAGACTGAAATCGGGTTTAATGGATGGCGATTTGATTTTGTCAAGGGATACGCTCCAGAATTCACCAAGTTGTTTGTGACAAACACGAGGCCTAGTTTCTCAGTAGGGGAACTTTGGAACTCCCTTGCTTATGGAAGTGATGGAAAACTCGAGTACAATCAAGATGCTCACCGTCGAGCACTTGTGGGATGGGTAGAGGGTGCTGGTGGCGATGTTACAGCATTCGATTTTACTACGAAAGGGATACTTCAAGCTGCAGTCCAAGGGGAACTATGGCGGATGAAAGATTCGAATGGAGGGGCACCAGGAATGATTGGCTTGTCACCAGGAAAGAGTGTAACTTTCGTAGATAATCATGATACAGGTTCTACACAGAACATGTGGCCATTTCCATCAGATAAAGTCATGCAGGGATATGCATACATTCTTACTCATCCGGGAATCCCCTCAATCTTTTATGACCACTACTTTGACTGGGGCTTGAAAGAGGAGATCACTAAACTCGTTGCAATCAGATTAAGGAACGGGGTCGGACCTGACAGTGCCTTGCGTATTCTAGCATCTGATGCAGATCTCTATGTAGCTGCCACAGATGAGAAAATCATAGCAAAGATTGGTCCAAGGTATGACGTGGGGAACCTTGTACCTCCAACCTACCAAATTGCAACTTCTGGAAATGATTACTGTGTGTGGGAGAAAAAAGCAGGAGCCTTGTTGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

433

Amino Acids

48.01

Weight (kDa)

5.2

Isoelectric Point (pI)

30.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	55 - 122	3.6e-07	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	366 - 424	9.7e-20	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001929)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25000
fragaria_vesca	FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030
malus_domestica	MD00G1035200.v1.1 MD05G1081100.v1.1 MD05G1081200.v1.1
prunus_persica	Prupe.8G125500_v2.0.a1
pyrus_communis	pycom05g07460 pycom10g07850
rosa_chinensis	RchiOBHm_Chr6g0257251 RchiOBHm_Chr6g0257281 RchiOBHm_Chr6g0257311
rosa_laevigata	RLG00000014683 RLG00000014684 RLG00000014685 RLG00000014687
rosa_multiflora	Rmu_sc0000820.1_g000005 Rmu_sc0002648.1_g000009 Rmu_sc0008660.1_g000002
rosa_roxburghii	Rroxscaffold_7G00208570 Rroxscaffold_7G00208600 Rroxscaffold_7G00208610
rosa_rugosa	Rorug04G0263800 Rorug05G0573700 Rorug05G0573800 Rorug05G0574000
rosa_samantha	Rh6AG090900 Rh6AG091400 Rh6AG091700 Rh6BG082600 Rh6BG082700 Rh6CG078700 Rh6CG079000 Rh6CG079200 Rh6DG074500 Rh6DG074800
rosa_wichuraiana	Rw6G007930 Rw6G007940 Rw6G007950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	877
AccI	GTMKAC	1 cut(s)	43
AciI	CCGC	1 cut(s)	853
AclWI	GGATC	1 cut(s)	70
AcoI	YGGCCR	1 cut(s)	959
AcsI	RAATTY	1 cut(s)	635
AcuI	CTGAAG	1 cut(s)	487
AfaI	GTAC	3 cut(s)	128, 731, 1221
AfiI	CCNNNNNNNGG	3 cut(s)	85, 427, 491
AflIII	ACRYGT	1 cut(s)	954
AgsI	TTSAA	3 cut(s)	94, 827, 1060
AjiI	CACGTC	1 cut(s)	1207
AjnI	CCWGG	3 cut(s)	232, 880, 901
AleI	CACNNNNGTG	1 cut(s)	354
AluBI	AGCT	7 cut(s)	8, 164, 461, 497, 553, 830, 1160
AluI	AGCT	7 cut(s)	8, 164, 461, 497, 553, 830, 1160
Alw21I	GWGCWC	2 cut(s)	555, 757
Alw26I	GTCTC	1 cut(s)	441
AlwI	GGATC	1 cut(s)	70
Ama87I	CYCGRG	1 cut(s)	725
AoxI	GGCC	3 cut(s)	430, 665, 959
ApaI	GGGCCC	1 cut(s)	434
ApeKI	GCWGC	4 cut(s)	447, 509, 830, 1160
ApoI	RAATTY	1 cut(s)	635
ArsI	GACNNNNNNTTYG	2 cut(s)	1028, 1060
Asp700I	GAANNNNTTC	1 cut(s)	16
AspLEI	GCGC	1 cut(s)	292
AspS9I	GGNCC	4 cut(s)	430, 431, 1109, 1193
AsuC2I	CCSGG	1 cut(s)	1014
AsuHPI	GGTGA	4 cut(s)	207, 631, 737, 891
AsuII	TTCGAA	2 cut(s)	396, 866
AvaI	CYCGRG	1 cut(s)	725
AvaII	GGWCC	2 cut(s)	1109, 1193
BaeGI	GKGCMC	2 cut(s)	434, 880
BalI	TGGCCA	1 cut(s)	961
BanI	GGYRCC	1 cut(s)	877
BanII	GRGCYC	2 cut(s)	434, 555
BauI	CACGAG	1 cut(s)	661
Bbv12I	GWGCWC	2 cut(s)	555, 757
BbvI	GCAGC	4 cut(s)	459, 496, 817, 1147
BccI	CCATC	5 cut(s)	117, 594, 710, 759, 976
BciT130I	CCWGG	3 cut(s)	234, 882, 903
BciVI	GTATCC	4 cut(s)	218, 312, 617, 813
BclI	TGATCA	1 cut(s)	523
BcnI	CCSGG	1 cut(s)	1014
BcoDI	GTCTC	1 cut(s)	441
BfaI	CTAG	3 cut(s)	161, 669, 1133
BfmI	CTRYAG	1 cut(s)	831
BfoI	RGCGCY	1 cut(s)	293
BfuI	GTATCC	4 cut(s)	218, 312, 617, 813
BglI	GCCNNNNNGGC	1 cut(s)	238
BglII	AGATCT	1 cut(s)	1147
BisI	GCNGC	4 cut(s)	448, 510, 831, 1161
BlsI	GCNGC	4 cut(s)	449, 511, 832, 1162
Bme1390I	CCNGG	4 cut(s)	234, 882, 903, 1014
Bme18I	GGWCC	2 cut(s)	1109, 1193
BmeT110I	CYCGRG	1 cut(s)	725
BmgBI	CACGTC	1 cut(s)	1207
BmgT120I	GGNCC	4 cut(s)	430, 431, 1109, 1193
BmiI	GGNNCC	5 cut(s)	431, 432, 879, 1214, 1285
BmrFI	CCNGG	4 cut(s)	234, 882, 903, 1014
BmrI	ACTGGG	1 cut(s)	1060
BmsI	GCATC	5 cut(s)	266, 399, 730, 1132, 1145
BmuI	ACTGGG	1 cut(s)	1060
BpmI	CTGGAG	3 cut(s)	393, 513, 615
Bpu14I	TTCGAA	2 cut(s)	396, 866
BpuEI	CTTGAG	2 cut(s)	204, 269
BpuMI	CCSGG	1 cut(s)	1014
BsaBI	GATNNNNATC	1 cut(s)	1146
BsaJI	CCNNGG	2 cut(s)	838, 1196
BsaXI	ACNNNNNCTCC	2 cut(s)	36, 66
Bsc4I	CCNNNNNNNGG	3 cut(s)	85, 427, 491
Bse1I	ACTGG	3 cut(s)	142, 496, 1055
Bse3DI	GCAATG	1 cut(s)	327
Bse8I	GATNNNNATC	1 cut(s)	1146
BseBI	CCWGG	3 cut(s)	234, 882, 903
BseDI	CCNNGG	2 cut(s)	838, 1196
BseGI	GGATG	6 cut(s)	128, 570, 605, 770, 861, 1009
BseJI	GATNNNNATC	1 cut(s)	1146
BseLI	CCNNNNNNNGG	3 cut(s)	85, 427, 491
BseMI	GCAATG	1 cut(s)	327
BseMII	CTCAG	2 cut(s)	570, 690
BseNI	ACTGG	3 cut(s)	142, 496, 1055
BseRI	GAGGAG	1 cut(s)	1079
BseSI	GKGCMC	2 cut(s)	434, 880
BseXI	GCAGC	4 cut(s)	459, 496, 817, 1147
BsgI	GTGCAG	1 cut(s)	378
BshFI	GGCC	3 cut(s)	432, 667, 961
BshNI	GGYRCC	1 cut(s)	877
BsiHKAI	GWGCWC	2 cut(s)	555, 757
BsiHKCI	CYCGRG	1 cut(s)	725
BsiSI	CCGG	1 cut(s)	1013
BslI	CCNNNNNNNGG	3 cut(s)	85, 427, 491
BsmAI	GTCTC	1 cut(s)	441
BsmBI	CGTCTC	1 cut(s)	441
BsmI	GAATGC	2 cut(s)	259, 797
BsnI	GGCC	3 cut(s)	432, 667, 961
BsoBI	CYCGRG	1 cut(s)	725
Bsp119I	TTCGAA	2 cut(s)	396, 866
Bsp120I	GGGCCC	1 cut(s)	430
Bsp1286I	GDGCHC	4 cut(s)	434, 555, 757, 880
Bsp143I	GATC	6 cut(s)	75, 247, 415, 523, 1068, 1147
BspACI	CCGC	1 cut(s)	853
BspANI	GGCC	3 cut(s)	432, 667, 961
BspCNI	CTCAG	2 cut(s)	569, 689
BspHI	TCATGA	1 cut(s)	931
BspLI	GGNNCC	5 cut(s)	431, 432, 879, 1214, 1285
BspMAI	CTGCAG	1 cut(s)	835
BspPI	GGATC	1 cut(s)	70
BspT104I	TTCGAA	2 cut(s)	396, 866
BspT107I	GGYRCC	1 cut(s)	877
BsrDI	GCAATG	1 cut(s)	327
BsrI	ACTGG	3 cut(s)	142, 496, 1055
BssECI	CCNNGG	2 cut(s)	838, 1196
BssMI	GATC	6 cut(s)	75, 247, 415, 523, 1068, 1147
BssNAI	GTATAC	1 cut(s)	44
BssSI	CACGAG	1 cut(s)	661
BssT1I	CCWWGG	2 cut(s)	838, 1196
Bst1107I	GTATAC	1 cut(s)	44
Bst2BI	CACGAG	1 cut(s)	661
Bst2UI	CCWGG	3 cut(s)	234, 882, 903
Bst4CI	ACNGT	3 cut(s)	749, 1118, 1262
Bst6I	CTCTTC	1 cut(s)	29
BstAPI	GCANNNNNTGC	1 cut(s)	1142
BstBI	TTCGAA	2 cut(s)	396, 866
BstDEI	CTNAG	2 cut(s)	556, 676
BstENI	CCTNNNNNAGG	1 cut(s)	83
BstF5I	GGATG	6 cut(s)	128, 570, 605, 770, 861, 1009
BstH2I	RGCGCY	1 cut(s)	293
BstHHI	GCGC	1 cut(s)	292
BstKTI	GATC	6 cut(s)	78, 250, 418, 526, 1071, 1150
BstMAI	GTCTC	1 cut(s)	441
BstMBI	GATC	6 cut(s)	75, 247, 415, 523, 1068, 1147
BstMWI	GCNNNNNNNGC	3 cut(s)	187, 238, 1142
BstNI	CCWGG	3 cut(s)	234, 882, 903
BstNSI	RCATGY	3 cut(s)	185, 232, 958
BstSCI	CCNGG	4 cut(s)	232, 880, 901, 1012
BstSFI	CTRYAG	1 cut(s)	831
BstSLI	GKGCMC	2 cut(s)	434, 880
BstV1I	GCAGC	4 cut(s)	459, 496, 817, 1147
BstX2I	RGATCY	2 cut(s)	75, 1147
BstYI	RGATCY	2 cut(s)	75, 1147
BstZ17I	GTATAC	1 cut(s)	44
BsuI	GTATCC	4 cut(s)	218, 312, 617, 813
BsuRI	GGCC	3 cut(s)	432, 667, 961
BtgZI	GCGATG	2 cut(s)	465, 801
BtrI	CACGTC	1 cut(s)	1207
BtsCI	GGATG	6 cut(s)	128, 570, 605, 770, 861, 1009
BtsIMutI	CAGTG	3 cut(s)	489, 511, 1123
CciI	TCATGA	1 cut(s)	931
CfoI	GCGC	1 cut(s)	292
Cfr13I	GGNCC	4 cut(s)	430, 431, 1109, 1193
Csp6I	GTAC	3 cut(s)	127, 730, 1220
CviAII	CATG	7 cut(s)	105, 182, 229, 308, 932, 955, 982
CviQI	GTAC	3 cut(s)	127, 730, 1220
DdeI	CTNAG	2 cut(s)	556, 676
DpnI	GATC	6 cut(s)	77, 249, 417, 525, 1070, 1149
DpnII	GATC	6 cut(s)	75, 247, 415, 523, 1068, 1147
EaeI	YGGCCR	1 cut(s)	959
Eam1104I	CTCTTC	1 cut(s)	29
EarI	CTCTTC	1 cut(s)	29
EciI	GGCGGA	1 cut(s)	868
Ecl136II	GAGCTC	1 cut(s)	553
Eco130I	CCWWGG	2 cut(s)	838, 1196
Eco147I	AGGCCT	1 cut(s)	667
Eco24I	GRGCYC	2 cut(s)	434, 555
Eco47I	GGWCC	2 cut(s)	1109, 1193
Eco53kI	GAGCTC	1 cut(s)	553
Eco57I	CTGAAG	1 cut(s)	487
Eco88I	CYCGRG	1 cut(s)	725
EcoICRI	GAGCTC	1 cut(s)	553
EcoNI	CCTNNNNNAGG	1 cut(s)	83
EcoO109I	RGGNCCY	1 cut(s)	430
EcoRI	GAATTC	1 cut(s)	635
EcoRII	CCWGG	3 cut(s)	232, 880, 901
EcoT14I	CCWWGG	2 cut(s)	838, 1196
EcoT22I	ATGCAT	2 cut(s)	259, 997
EcoT38I	GRGCYC	2 cut(s)	434, 555
ErhI	CCWWGG	2 cut(s)	838, 1196
Esp3I	CGTCTC	1 cut(s)	441
FaeI	CATG	7 cut(s)	108, 185, 232, 311, 935, 958, 985
FalI	AAGNNNNNCTT	2 cut(s)	807, 839
FatI	CATG	7 cut(s)	104, 181, 228, 307, 931, 954, 981
FbaI	TGATCA	1 cut(s)	523
FblI	GTMKAC	1 cut(s)	43
Fnu4HI	GCNGC	4 cut(s)	448, 510, 831, 1161
FokI	GGATG	6 cut(s)	135, 577, 612, 777, 868, 996
FriOI	GRGCYC	2 cut(s)	434, 555
Fsp4HI	GCNGC	4 cut(s)	448, 510, 831, 1161
FspBI	CTAG	3 cut(s)	161, 669, 1133
GlaI	GCGC	1 cut(s)	291
GluI	GCNGC	4 cut(s)	448, 510, 831, 1161
GsuI	CTGGAG	3 cut(s)	393, 513, 615
HaeII	RGCGCY	1 cut(s)	293
HaeIII	GGCC	3 cut(s)	432, 667, 961
HapII	CCGG	1 cut(s)	1013
HhaI	GCGC	1 cut(s)	292
Hin1II	CATG	7 cut(s)	108, 185, 232, 311, 935, 958, 985
Hin6I	GCGC	1 cut(s)	290
HinP1I	GCGC	1 cut(s)	290
HinfI	GANTC	4 cut(s)	101, 540, 863, 1017
HpaII	CCGG	1 cut(s)	1013
HphI	GGTGA	4 cut(s)	207, 631, 737, 891
Hpy166II	GTNNAC	1 cut(s)	44
Hpy188I	TCNGA	6 cut(s)	252, 559, 973, 1091, 1109, 1141
Hpy188III	TCNNGA	8 cut(s)	261, 410, 515, 544, 632, 737, 932, 1248
Hpy8I	GTNNAC	1 cut(s)	44
Hpy99I	CGWCG	1 cut(s)	753
HpyAV	CCTTC	6 cut(s)	103, 110, 210, 231, 313, 392
HpyCH4III	ACNGT	3 cut(s)	749, 1118, 1262
HpyCH4IV	ACGT	1 cut(s)	1206
HpyF10VI	GCNNNNNNNGC	3 cut(s)	187, 238, 1142
HpyF3I	CTNAG	2 cut(s)	556, 676
HpySE526I	ACGT	1 cut(s)	1206
Hsp92II	CATG	7 cut(s)	108, 185, 232, 311, 935, 958, 985
HspAI	GCGC	1 cut(s)	290
Ksp22I	TGATCA	1 cut(s)	523
Kzo9I	GATC	6 cut(s)	75, 247, 415, 523, 1068, 1147
LmnI	GCTCC	5 cut(s)	73, 494, 517, 634, 1283
Lsp1109I	GCAGC	4 cut(s)	459, 496, 817, 1147
LweI	GCATC	5 cut(s)	266, 399, 730, 1132, 1145
MaeI	CTAG	3 cut(s)	161, 669, 1133
MaeII	ACGT	1 cut(s)	1206
MaeIII	GTNAC	4 cut(s)	652, 790, 897, 913
MalI	GATC	6 cut(s)	77, 249, 417, 525, 1070, 1149
MboI	GATC	6 cut(s)	75, 247, 415, 523, 1068, 1147
MboII	GAAGA	4 cut(s)	16, 159, 376, 385
MfeI	CAATTG	1 cut(s)	94
MflI	RGATCY	2 cut(s)	75, 1147
MhlI	GDGCHC	4 cut(s)	434, 555, 757, 880
MlsI	TGGCCA	1 cut(s)	961
MluCI	AATT	4 cut(s)	94, 568, 635, 1236
MluNI	TGGCCA	1 cut(s)	961
MlyI	GAGTC	1 cut(s)	549
MmeI	TCCRAC	3 cut(s)	1087, 1250, 1272
Mox20I	TGGCCA	1 cut(s)	961
Mph1103I	ATGCAT	2 cut(s)	259, 997
MroXI	GAANNNNTTC	1 cut(s)	16
MscI	TGGCCA	1 cut(s)	961
MseI	TTAA	5 cut(s)	62, 137, 531, 594, 1094
MslI	CAYNNNNRTG	1 cut(s)	354
Msp20I	TGGCCA	1 cut(s)	961
MspI	CCGG	1 cut(s)	1013
MspR9I	CCNGG	4 cut(s)	234, 882, 903, 1014
MunI	CAATTG	1 cut(s)	94
Mva1269I	GAATGC	2 cut(s)	259, 797
MvaI	CCWGG	3 cut(s)	234, 882, 903
MwoI	GCNNNNNNNGC	3 cut(s)	187, 238, 1142
NciI	CCSGG	1 cut(s)	1014
NdeII	GATC	6 cut(s)	75, 247, 415, 523, 1068, 1147
NlaIII	CATG	7 cut(s)	108, 185, 232, 311, 935, 958, 985
NlaIV	GGNNCC	5 cut(s)	431, 432, 879, 1214, 1285
NmuCI	GTSAC	2 cut(s)	652, 897
NsiI	ATGCAT	2 cut(s)	259, 997
NspI	RCATGY	3 cut(s)	185, 232, 958
NspV	TTCGAA	2 cut(s)	396, 866
OliI	CACNNNNGTG	1 cut(s)	354
PaeR7I	CTCGAG	1 cut(s)	725
PagI	TCATGA	1 cut(s)	931
PceI	AGGCCT	1 cut(s)	667
PciI	ACATGT	1 cut(s)	954
PctI	GAATGC	2 cut(s)	259, 797
PdmI	GAANNNNTTC	1 cut(s)	16
PfeI	GAWTC	3 cut(s)	101, 863, 1017
PfoI	TCCNGGA	1 cut(s)	1012
PkrI	GCNGC	4 cut(s)	449, 511, 832, 1162
PleI	GAGTC	1 cut(s)	548
PpsI	GAGTC	1 cut(s)	548
PscI	ACATGT	1 cut(s)	954
Psp124BI	GAGCTC	1 cut(s)	555
Psp6I	CCWGG	3 cut(s)	232, 880, 901
PspGI	CCWGG	3 cut(s)	232, 880, 901
PspN4I	GGNNCC	5 cut(s)	431, 432, 879, 1214, 1285
PspOMI	GGGCCC	1 cut(s)	430
PspPI	GGNCC	4 cut(s)	430, 431, 1109, 1193
PspXI	VCTCGAGB	1 cut(s)	725
PstI	CTGCAG	1 cut(s)	835
PsuI	RGATCY	2 cut(s)	75, 1147
RsaI	GTAC	3 cut(s)	128, 731, 1221
RsaNI	GTAC	3 cut(s)	127, 730, 1220
RseI	CAYNNNNRTG	1 cut(s)	354
SacI	GAGCTC	1 cut(s)	555
SaqAI	TTAA	5 cut(s)	62, 137, 531, 594, 1094
SatI	GCNGC	4 cut(s)	448, 510, 831, 1161
Sau3AI	GATC	6 cut(s)	75, 247, 415, 523, 1068, 1147
Sau96I	GGNCC	4 cut(s)	430, 431, 1109, 1193
SchI	GAGTC	1 cut(s)	549
ScrFI	CCNGG	4 cut(s)	234, 882, 903, 1014
SduI	GDGCHC	4 cut(s)	434, 555, 757, 880
SfaNI	GCATC	5 cut(s)	266, 399, 730, 1132, 1145
SfcI	CTRYAG	1 cut(s)	831
Sfr274I	CTCGAG	1 cut(s)	725
SfuI	TTCGAA	2 cut(s)	396, 866
SinI	GGWCC	2 cut(s)	1109, 1193
SlaI	CTCGAG	1 cut(s)	725
SmiMI	CAYNNNNRTG	1 cut(s)	354
SmlI	CTYRAG	3 cut(s)	219, 284, 725
SmoI	CTYRAG	3 cut(s)	219, 284, 725
Sse9I	AATT	4 cut(s)	94, 568, 635, 1236
SseBI	AGGCCT	1 cut(s)	667
SsiI	CCGC	1 cut(s)	853
SspMI	CTAG	3 cut(s)	161, 669, 1133
SstI	GAGCTC	1 cut(s)	555
StuI	AGGCCT	1 cut(s)	667
StyD4I	CCNGG	4 cut(s)	232, 880, 901, 1012
StyI	CCWWGG	2 cut(s)	838, 1196
TaaI	ACNGT	3 cut(s)	749, 1118, 1262
TaiI	ACGT	1 cut(s)	1209
TaqI	TCGA	6 cut(s)	10, 396, 726, 751, 801, 866
TasI	AATT	4 cut(s)	94, 568, 635, 1236
TatI	WGTACW	1 cut(s)	729
TfiI	GAWTC	3 cut(s)	101, 863, 1017
Tru1I	TTAA	5 cut(s)	62, 137, 531, 594, 1094
Tru9I	TTAA	5 cut(s)	62, 137, 531, 594, 1094
TscAI	CASTG	3 cut(s)	496, 511, 1123
TseFI	GTSAC	2 cut(s)	652, 897
TseI	GCWGC	4 cut(s)	447, 509, 830, 1160
Tsp45I	GTSAC	2 cut(s)	652, 897
TspDTI	ATGAA	5 cut(s)	17, 294, 387, 581, 872
TspGWI	ACGGA	1 cut(s)	282
TspRI	CASTG	3 cut(s)	496, 511, 1123
VpaK11BI	GGWCC	2 cut(s)	1109, 1193
XagI	CCTNNNNNAGG	1 cut(s)	83
XapI	RAATTY	1 cut(s)	635
XceI	RCATGY	3 cut(s)	185, 232, 958
XhoI	CTCGAG	1 cut(s)	725
XmiI	GTMKAC	1 cut(s)	43
XmnI	GAANNNNTTC	1 cut(s)	16
XspI	CTAG	3 cut(s)	161, 669, 1133
Zsp2I	ATGCAT	2 cut(s)	259, 997

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.