Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001929

Physical Location & Seq

Genomic Coordinates Reverse (-)

16939645 .. 16942733

Gene Structure 3089 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1081100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1530 bp

ATGTGTAGATTCTCTGGAAGCAACAATTTGGCCGAAGTCAGGACAGTGTTAATGTTGCCTGATTTGATTCAAGACCTCCTGCAAAGATTCTTATCGATGGAGAATCCAGTTAAACCGTCATTGCTAGCAATCTCGAAGAGTTTCCCTCTAACCAGGAGGCCAATCACAGCACGACACGTGTCGACATCAAAAGCCAATCATAGCGCGACACGTGTCAACATCAGAAGCCAATCACAACACGACATGTGTCAATACATGAACACTCGAAACGTTCCCCTCATCTGTTTCCTACTCTCTATCCTTCTCACACCCAACTTCACTGCTTCACAGATCATCTTTCAGGGGTTCAACTGGGAATCAAGCAGGAAGGGAGGATGGTACAACATTTTAAAGCAGTCTGTACAAGACCTAGCTAGTTCCGGAATCACACATGTTTGGCTTCCTCCATCTTCTCATGCTGCTTCAGATGAAGGATACATGCCGGGAAGGCTTTATGATCTCAATTCAAAATTTGGAAACAAAGATGAATTGAAGTCACTGGTAAGTGCGTACCGCAACAGTGGGATACAGTCTGTGGCAGATATAGTTATAAACCACAGAACTGCAGAGAAGCAAGACGAGAGAGGAATATGGTGCATCTTTGAAGGAGGAACTGACGATGATCGCCTTGATTGGGGGCCGTCTCTTATTTGCGGTGATGATACAAAATATTCTGATGGCAAGGGAAACCCTGACACTGGAGAGGACTTCGGTGGTGCTCCTGACATTGACCACAAAAACACAAGGGTTCAGAAAGAGCTCTCGGATTGGATGAATTGGTTAAAGACTGAAATCGGGTTCAGTGGTTGGAGATTTGATTTTGTTAAGGGATATGCTCCTGAATATACCAAGCTGTATATGACAAATACAAGTCCAAATTTCGCAGTAGGGGAACTTTGGAATTCCCTTTCTTATGGAAGTGATGGAAAACCGAACCCAGATCAAGATGCACATCGACAAGCGCTAGTCGGATGGGTGGAGAGTGCTGGAGGAGGTGTTACAGCATTTGATTTTACAACTAAAGGGATACTTCAAGCTGCAGTCCAAGGGGAGCTTTGGAGGCTGAAGGACTCGAATGGTGGCGCTCCAGGGATGATCGGATTGAAGCCAGGAAGTAGTGTAACTTTCATTGACAATCATGATACAGGTTCCACACAACATTTGTGGCCATTTCCATCTGATAAGGTCATGCAGGGATATGCTTACATTCTCACTCATCCAGGGATCCCATCAATATTTTATGATCACTTCTTTGACTGGGGCTTGAAAGGGGAGATCTCTAAACTCACTGCAATCAGATCGAGAAATGGGATCAAACCCGACAGTGCCTTGCGCATTCTAGCAGCCGATGCAGATGTCTATGTAGCTTCCATAGATGAGAAAATCATAGTCAAGATTGGTCCGAAGATGGATTTAGGCCAACTTATACCTCCAAACTTCCAAGTTTCGACTTCTGGAAAGGATTATGCCGTGTGGGAGAGAAAGGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

510

Amino Acids

56.52

Weight (kDa)

6.32

Isoelectric Point (pI)

35.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	133 - 204	2.8e-08	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	448 - 506	1.8e-22	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001929)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25000
fragaria_vesca	FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030
malus_domestica	MD00G1035200.v1.1 MD05G1081100.v1.1 MD05G1081200.v1.1
prunus_persica	Prupe.8G125500_v2.0.a1
pyrus_communis	pycom05g07460 pycom10g07850
rosa_chinensis	RchiOBHm_Chr6g0257251 RchiOBHm_Chr6g0257281 RchiOBHm_Chr6g0257311
rosa_laevigata	RLG00000014683 RLG00000014684 RLG00000014685 RLG00000014687
rosa_multiflora	Rmu_sc0000820.1_g000005 Rmu_sc0002648.1_g000009 Rmu_sc0008660.1_g000002
rosa_roxburghii	Rroxscaffold_7G00208570 Rroxscaffold_7G00208600 Rroxscaffold_7G00208610
rosa_rugosa	Rorug04G0263800 Rorug05G0573700 Rorug05G0573800 Rorug05G0574000
rosa_samantha	Rh6AG090900 Rh6AG091400 Rh6AG091700 Rh6BG082600 Rh6BG082700 Rh6CG078700 Rh6CG079000 Rh6CG079200 Rh6DG074500 Rh6DG074800
rosa_wichuraiana	Rw6G007930 Rw6G007940 Rw6G007950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	590
Acc16I	TGCGCA	1 cut(s)	1373
AccI	GTMKAC	1 cut(s)	182
AccII	CGCG	1 cut(s)	206
AccIII	TCCGGA	1 cut(s)	419
AciI	CCGC	2 cut(s)	553, 693
AclI	AACGTT	1 cut(s)	270
AclWI	GGATC	3 cut(s)	1258, 1271, 1358
AcoI	YGGCCR	2 cut(s)	30, 1205
AcsI	RAATTY	3 cut(s)	509, 916, 940
AcuI	CTGAAG	2 cut(s)	447, 1124
AcvI	CACGTG	2 cut(s)	178, 212
AfaI	GTAC	3 cut(s)	380, 402, 551
AfeI	AGCGCT	1 cut(s)	1002
AfiI	CCNNNNNNNGG	3 cut(s)	39, 673, 737
AflIII	ACRYGT	6 cut(s)	175, 177, 209, 211, 243, 430
AgsI	TTSAA	8 cut(s)	71, 349, 507, 532, 644, 1073, 1144, 1306
AjnI	CCWGG	4 cut(s)	152, 1126, 1147, 1258
AluBI	AGCT	6 cut(s)	413, 799, 892, 1076, 1093, 1406
AluI	AGCT	6 cut(s)	413, 799, 892, 1076, 1093, 1406
Alw21I	GWGCWC	2 cut(s)	760, 801
Alw26I	GTCTC	1 cut(s)	687
AlwI	GGATC	3 cut(s)	1258, 1271, 1358
Aor13HI	TCCGGA	1 cut(s)	419
Aor51HI	AGCGCT	1 cut(s)	1002
AoxI	GGCC	5 cut(s)	30, 158, 677, 1205, 1456
ApeKI	GCWGC	3 cut(s)	458, 1076, 1382
ApoI	RAATTY	3 cut(s)	509, 916, 940
Asp700I	GAANNNNTTC	1 cut(s)	140
AspLEI	GCGC	4 cut(s)	206, 1003, 1124, 1374
AspS9I	GGNCC	2 cut(s)	677, 1439
AsuC2I	CCSGG	1 cut(s)	483
AsuHPI	GGTGA	1 cut(s)	707
AsuNHI	GCTAGC	1 cut(s)	124
AvaII	GGWCC	1 cut(s)	1439
BalI	TGGCCA	1 cut(s)	1207
BamHI	GGATCC	1 cut(s)	1263
BanII	GRGCYC	1 cut(s)	801
BbrPI	CACGTG	2 cut(s)	178, 212
Bbv12I	GWGCWC	2 cut(s)	760, 801
BbvI	GCAGC	3 cut(s)	445, 1063, 1394
BccI	CCATC	9 cut(s)	91, 369, 454, 710, 956, 1005, 1222, 1276, 1441
BceAI	ACGGC	2 cut(s)	664, 1493
BcgI	CGANNNNNNTGC	2 cut(s)	1320, 1354
BciT130I	CCWGG	4 cut(s)	154, 1128, 1149, 1260
BciVI	GTATCC	3 cut(s)	467, 558, 1059
BclI	TGATCA	1 cut(s)	1282
BcnI	CCSGG	1 cut(s)	483
BcoDI	GTCTC	1 cut(s)	687
BfaI	CTAG	5 cut(s)	125, 410, 414, 1004, 1379
BfmI	CTRYAG	2 cut(s)	603, 1077
BfoI	RGCGCY	2 cut(s)	1004, 1125
BfuI	GTATCC	3 cut(s)	467, 558, 1059
BglI	GCCNNNNNGGC	1 cut(s)	487
BglII	AGATCT	1 cut(s)	1314
BisI	GCNGC	3 cut(s)	459, 1077, 1383
BlsI	GCNGC	3 cut(s)	460, 1078, 1384
Bme1390I	CCNGG	5 cut(s)	154, 483, 1128, 1149, 1260
Bme18I	GGWCC	1 cut(s)	1439
BmgT120I	GGNCC	2 cut(s)	677, 1439
BmiI	GGNNCC	3 cut(s)	678, 1189, 1265
BmrFI	CCNGG	5 cut(s)	154, 483, 1128, 1149, 1260
BmrI	ACTGGG	2 cut(s)	361, 1306
BmsI	GCATC	3 cut(s)	645, 976, 1378
BmtI	GCTAGC	1 cut(s)	128
BmuI	ACTGGG	2 cut(s)	361, 1306
BoxI	GACNNNNGTC	3 cut(s)	178, 212, 246
BplI	GAGNNNNNCTC	2 cut(s)	130, 162
BpmI	CTGGAG	3 cut(s)	759, 1047, 1110
BpuMI	CCSGG	1 cut(s)	483
Bsa29I	ATCGAT	1 cut(s)	95
BsaAI	YACGTR	2 cut(s)	178, 212
BsaBI	GATNNNNATC	2 cut(s)	91, 990
BsaJI	CCNNGG	3 cut(s)	1084, 1127, 1259
BsaWI	WCCGGW	1 cut(s)	419
BsaXI	ACNNNNNCTCC	2 cut(s)	1020, 1050
Bsc4I	CCNNNNNNNGG	3 cut(s)	39, 673, 737
Bse1I	ACTGG	5 cut(s)	107, 356, 543, 742, 1301
Bse3DI	GCAATG	1 cut(s)	119
Bse8I	GATNNNNATC	2 cut(s)	91, 990
BseAI	TCCGGA	1 cut(s)	419
BseBI	CCWGG	4 cut(s)	154, 1128, 1149, 1260
BseCI	ATCGAT	1 cut(s)	95
BseDI	CCNNGG	3 cut(s)	1084, 1127, 1259
BseGI	GGATG	5 cut(s)	380, 816, 1016, 1137, 1255
BseJI	GATNNNNATC	2 cut(s)	91, 990
BseLI	CCNNNNNNNGG	3 cut(s)	39, 673, 737
BseMI	GCAATG	1 cut(s)	119
BseNI	ACTGG	5 cut(s)	107, 356, 543, 742, 1301
BseRI	GAGGAG	1 cut(s)	1044
BseXI	GCAGC	3 cut(s)	445, 1063, 1394
Bsh1236I	CGCG	1 cut(s)	206
BshFI	GGCC	5 cut(s)	32, 160, 679, 1207, 1458
BshVI	ATCGAT	1 cut(s)	95
BsiHKAI	GWGCWC	2 cut(s)	760, 801
BsiSI	CCGG	2 cut(s)	420, 482
BslI	CCNNNNNNNGG	3 cut(s)	39, 673, 737
BsmAI	GTCTC	1 cut(s)	687
BsmBI	CGTCTC	1 cut(s)	687
BsmI	GAATGC	1 cut(s)	1374
BsnI	GGCC	5 cut(s)	32, 160, 679, 1207, 1458
Bsp1286I	GDGCHC	2 cut(s)	760, 801
Bsp13I	TCCGGA	1 cut(s)	419
Bsp1407I	TGTACA	1 cut(s)	400
BspACI	CCGC	2 cut(s)	553, 693
BspANI	GGCC	5 cut(s)	32, 160, 679, 1207, 1458
BspDI	ATCGAT	1 cut(s)	95
BspEI	TCCGGA	1 cut(s)	419
BspFNI	CGCG	1 cut(s)	206
BspHI	TCATGA	1 cut(s)	1177
BspLI	GGNNCC	3 cut(s)	678, 1189, 1265
BspMAI	CTGCAG	2 cut(s)	607, 1081
BspOI	GCTAGC	1 cut(s)	128
BspPI	GGATC	3 cut(s)	1258, 1271, 1358
BsrDI	GCAATG	1 cut(s)	119
BsrGI	TGTACA	1 cut(s)	400
BsrI	ACTGG	5 cut(s)	107, 356, 543, 742, 1301
BssECI	CCNNGG	3 cut(s)	1084, 1127, 1259
BssT1I	CCWWGG	1 cut(s)	1084
Bst2UI	CCWGG	4 cut(s)	154, 1128, 1149, 1260
Bst4CI	ACNGT	5 cut(s)	46, 117, 560, 570, 1364
Bst6I	CTCTTC	1 cut(s)	131
BstAPI	GCANNNNNTGC	1 cut(s)	1388
BstAUI	TGTACA	1 cut(s)	400
BstBAI	YACGTR	2 cut(s)	178, 212
BstC8I	GCNNGC	1 cut(s)	126
BstF5I	GGATG	5 cut(s)	380, 816, 1016, 1137, 1255
BstFNI	CGCG	1 cut(s)	206
BstH2I	RGCGCY	2 cut(s)	1004, 1125
BstHHI	GCGC	4 cut(s)	206, 1003, 1124, 1374
BstMAI	GTCTC	1 cut(s)	687
BstMWI	GCNNNNNNNGC	3 cut(s)	487, 1099, 1388
BstNI	CCWGG	4 cut(s)	154, 1128, 1149, 1260
BstNSI	RCATGY	3 cut(s)	247, 434, 481
BstPAI	GACNNNNGTC	3 cut(s)	178, 212, 246
BstSCI	CCNGG	5 cut(s)	152, 481, 1126, 1147, 1258
BstSFI	CTRYAG	2 cut(s)	603, 1077
BstUI	CGCG	1 cut(s)	206
BstV1I	GCAGC	3 cut(s)	445, 1063, 1394
BstX2I	RGATCY	2 cut(s)	1263, 1314
BstYI	RGATCY	2 cut(s)	1263, 1314
Bsu15I	ATCGAT	1 cut(s)	95
BsuI	GTATCC	3 cut(s)	467, 558, 1059
BsuRI	GGCC	5 cut(s)	32, 160, 679, 1207, 1458
BsuTUI	ATCGAT	1 cut(s)	95
BtsCI	GGATG	5 cut(s)	380, 816, 1016, 1137, 1255
BtsI	GCAGTG	2 cut(s)	318, 1326
BtsIMutI	CAGTG	8 cut(s)	51, 318, 536, 565, 735, 847, 1326, 1369
Cac8I	GCNNGC	1 cut(s)	126
CciI	TCATGA	1 cut(s)	1177
CfoI	GCGC	4 cut(s)	206, 1003, 1124, 1374
Cfr13I	GGNCC	2 cut(s)	677, 1439
ClaI	ATCGAT	1 cut(s)	95
Csp6I	GTAC	3 cut(s)	379, 401, 550
CspCI	CAANNNNNGTGG	2 cut(s)	1184, 1219
CviAII	CATG	8 cut(s)	244, 256, 431, 455, 478, 1178, 1228, 1527
CviQI	GTAC	3 cut(s)	379, 401, 550
DraI	TTTAAA	1 cut(s)	390
EaeI	YGGCCR	2 cut(s)	30, 1205
Eam1104I	CTCTTC	1 cut(s)	131
EarI	CTCTTC	1 cut(s)	131
Ecl136II	GAGCTC	1 cut(s)	799
Eco130I	CCWWGG	1 cut(s)	1084
Eco24I	GRGCYC	1 cut(s)	801
Eco47I	GGWCC	1 cut(s)	1439
Eco47III	AGCGCT	1 cut(s)	1002
Eco53kI	GAGCTC	1 cut(s)	799
Eco57I	CTGAAG	2 cut(s)	447, 1124
Eco72I	CACGTG	2 cut(s)	178, 212
EcoICRI	GAGCTC	1 cut(s)	799
EcoRI	GAATTC	1 cut(s)	940
EcoRII	CCWGG	4 cut(s)	152, 1126, 1147, 1258
EcoT14I	CCWWGG	1 cut(s)	1084
EcoT38I	GRGCYC	1 cut(s)	801
ErhI	CCWWGG	1 cut(s)	1084
Esp3I	CGTCTC	1 cut(s)	687
FaeI	CATG	8 cut(s)	247, 259, 434, 458, 481, 1181, 1231, 1530
FalI	AAGNNNNNCTT	4 cut(s)	1053, 1085, 1077, 1109
FatI	CATG	8 cut(s)	243, 255, 430, 454, 477, 1177, 1227, 1526
FbaI	TGATCA	1 cut(s)	1282
FblI	GTMKAC	1 cut(s)	182
Fnu4HI	GCNGC	3 cut(s)	459, 1077, 1383
FokI	GGATG	5 cut(s)	387, 823, 1023, 1144, 1242
FriOI	GRGCYC	1 cut(s)	801
Fsp4HI	GCNGC	3 cut(s)	459, 1077, 1383
FspBI	CTAG	5 cut(s)	125, 410, 414, 1004, 1379
FspI	TGCGCA	1 cut(s)	1373
GlaI	GCGC	4 cut(s)	205, 1002, 1123, 1373
GluI	GCNGC	3 cut(s)	459, 1077, 1383
GsuI	CTGGAG	3 cut(s)	759, 1047, 1110
HaeII	RGCGCY	2 cut(s)	1004, 1125
HaeIII	GGCC	5 cut(s)	32, 160, 679, 1207, 1458
HapII	CCGG	2 cut(s)	420, 482
HhaI	GCGC	4 cut(s)	206, 1003, 1124, 1374
Hin1II	CATG	8 cut(s)	247, 259, 434, 458, 481, 1181, 1231, 1530
Hin6I	GCGC	4 cut(s)	204, 1001, 1122, 1372
HinP1I	GCGC	4 cut(s)	204, 1001, 1122, 1372
HincII	GTYRAC	2 cut(s)	183, 217
HindII	GTYRAC	2 cut(s)	183, 217
HinfI	GANTC	7 cut(s)	9, 67, 87, 103, 356, 423, 1109
HpaII	CCGG	2 cut(s)	420, 482
HphI	GGTGA	1 cut(s)	707
Hpy166II	GTNNAC	2 cut(s)	183, 217
Hpy8I	GTNNAC	2 cut(s)	183, 217
HpyAV	CCTTC	6 cut(s)	311, 361, 464, 480, 638, 1099
HpyCH4III	ACNGT	5 cut(s)	46, 117, 560, 570, 1364
HpyCH4IV	ACGT	3 cut(s)	177, 211, 270
HpyCH4V	TGCA	8 cut(s)	82, 605, 636, 989, 1079, 1231, 1331, 1391
HpyF10VI	GCNNNNNNNGC	3 cut(s)	487, 1099, 1388
HpySE526I	ACGT	3 cut(s)	177, 211, 270
Hsp92II	CATG	8 cut(s)	247, 259, 434, 458, 481, 1181, 1231, 1530
HspAI	GCGC	4 cut(s)	204, 1001, 1122, 1372
Kpn2I	TCCGGA	1 cut(s)	419
Ksp22I	TGATCA	1 cut(s)	1282
LmnI	GCTCC	4 cut(s)	763, 880, 1090, 1129
Lsp1109I	GCAGC	3 cut(s)	445, 1063, 1394
LweI	GCATC	3 cut(s)	645, 976, 1378
MaeI	CTAG	5 cut(s)	125, 410, 414, 1004, 1379
MaeII	ACGT	3 cut(s)	177, 211, 270
MaeIII	GTNAC	3 cut(s)	534, 1036, 1159
MboII	GAAGA	3 cut(s)	148, 441, 1456
MflI	RGATCY	2 cut(s)	1263, 1314
MhlI	GDGCHC	2 cut(s)	760, 801
MlsI	TGGCCA	1 cut(s)	1207
MluCI	AATT	7 cut(s)	25, 502, 509, 527, 814, 916, 940
MluNI	TGGCCA	1 cut(s)	1207
MlyI	GAGTC	1 cut(s)	1103
MmeI	TCCRAC	2 cut(s)	827, 988
Mox20I	TGGCCA	1 cut(s)	1207
MroI	TCCGGA	1 cut(s)	419
MroXI	GAANNNNTTC	1 cut(s)	140
MscI	TGGCCA	1 cut(s)	1207
MseI	TTAA	5 cut(s)	50, 111, 389, 821, 864
Msp20I	TGGCCA	1 cut(s)	1207
MspI	CCGG	2 cut(s)	420, 482
MspR9I	CCNGG	5 cut(s)	154, 483, 1128, 1149, 1260
Mva1269I	GAATGC	1 cut(s)	1374
MvaI	CCWGG	4 cut(s)	154, 1128, 1149, 1260
MvnI	CGCG	1 cut(s)	206
MwoI	GCNNNNNNNGC	3 cut(s)	487, 1099, 1388
NciI	CCSGG	1 cut(s)	483
NheI	GCTAGC	1 cut(s)	124
NlaIII	CATG	8 cut(s)	247, 259, 434, 458, 481, 1181, 1231, 1530
NlaIV	GGNNCC	3 cut(s)	678, 1189, 1265
NmuCI	GTSAC	1 cut(s)	534
NsbI	TGCGCA	1 cut(s)	1373
NspI	RCATGY	3 cut(s)	247, 434, 481
PagI	TCATGA	1 cut(s)	1177
PciI	ACATGT	2 cut(s)	243, 430
PctI	GAATGC	1 cut(s)	1374
PdmI	GAANNNNTTC	1 cut(s)	140
PfeI	GAWTC	6 cut(s)	9, 67, 87, 103, 356, 423
PkrI	GCNGC	3 cut(s)	460, 1078, 1384
PleI	GAGTC	1 cut(s)	1103
PmaCI	CACGTG	2 cut(s)	178, 212
PmlI	CACGTG	2 cut(s)	178, 212
PpsI	GAGTC	1 cut(s)	1103
Ppu21I	YACGTR	2 cut(s)	178, 212
PscI	ACATGT	2 cut(s)	243, 430
PshAI	GACNNNNGTC	3 cut(s)	178, 212, 246
PsiI	TTATAA	1 cut(s)	590
Psp124BI	GAGCTC	1 cut(s)	801
Psp1406I	AACGTT	1 cut(s)	270
Psp6I	CCWGG	4 cut(s)	152, 1126, 1147, 1258
PspCI	CACGTG	2 cut(s)	178, 212
PspGI	CCWGG	4 cut(s)	152, 1126, 1147, 1258
PspN4I	GGNNCC	3 cut(s)	678, 1189, 1265
PspPI	GGNCC	2 cut(s)	677, 1439
PstI	CTGCAG	2 cut(s)	607, 1081
PsuI	RGATCY	2 cut(s)	1263, 1314
RsaI	GTAC	3 cut(s)	380, 402, 551
RsaNI	GTAC	3 cut(s)	379, 401, 550
SacI	GAGCTC	1 cut(s)	801
SalI	GTCGAC	1 cut(s)	181
SaqAI	TTAA	5 cut(s)	50, 111, 389, 821, 864
SatI	GCNGC	3 cut(s)	459, 1077, 1383
Sau96I	GGNCC	2 cut(s)	677, 1439
SchI	GAGTC	1 cut(s)	1103
ScrFI	CCNGG	5 cut(s)	154, 483, 1128, 1149, 1260
SduI	GDGCHC	2 cut(s)	760, 801
SfaNI	GCATC	3 cut(s)	645, 976, 1378
SfcI	CTRYAG	2 cut(s)	603, 1077
SinI	GGWCC	1 cut(s)	1439
Sse9I	AATT	7 cut(s)	25, 502, 509, 527, 814, 916, 940
SsiI	CCGC	2 cut(s)	553, 693
SspI	AATATT	2 cut(s)	710, 1275
SspMI	CTAG	5 cut(s)	125, 410, 414, 1004, 1379
SstI	GAGCTC	1 cut(s)	801
StyD4I	CCNGG	5 cut(s)	152, 481, 1126, 1147, 1258
StyI	CCWWGG	1 cut(s)	1084
TaaI	ACNGT	5 cut(s)	46, 117, 560, 570, 1364
TaiI	ACGT	3 cut(s)	180, 214, 273
TaqI	TCGA	8 cut(s)	95, 134, 182, 265, 994, 1112, 1340, 1487
TasI	AATT	7 cut(s)	25, 502, 509, 527, 814, 916, 940
TatI	WGTACW	1 cut(s)	400
TfiI	GAWTC	6 cut(s)	9, 67, 87, 103, 356, 423
Tru1I	TTAA	5 cut(s)	50, 111, 389, 821, 864
Tru9I	TTAA	5 cut(s)	50, 111, 389, 821, 864
TscAI	CASTG	8 cut(s)	51, 325, 543, 565, 742, 847, 1333, 1369
TseFI	GTSAC	1 cut(s)	534
TseI	GCWGC	3 cut(s)	458, 1076, 1382
Tsp45I	GTSAC	1 cut(s)	534
TspDTI	ATGAA	5 cut(s)	272, 483, 540, 827, 1156
TspRI	CASTG	8 cut(s)	51, 325, 543, 565, 742, 847, 1333, 1369
VpaK11BI	GGWCC	1 cut(s)	1439
XapI	RAATTY	3 cut(s)	509, 916, 940
XceI	RCATGY	3 cut(s)	247, 434, 481
XmiI	GTMKAC	1 cut(s)	182
XmnI	GAANNNNTTC	1 cut(s)	140
XspI	CTAG	5 cut(s)	125, 410, 414, 1004, 1379

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.