Rw6G007940

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001929
Physical Location & Seq
Forward (+)
13343559 .. 13346141
2583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G007940.1

Sequence Viewer

Length: 1056 bp
ATGCCTGGTAGGCTCTATGATCTCGCATCAAAATATGGAAGCCAAGATGAATTAAAGGCACTGGTAAAAACCTACCATGACAATGGGATACAGAGCATAGCAGATATTGTTATAAACCACAGATGTGCAGAGAAGAAAGATGAGAGAGGAATATGGTGCATTTTTGAAGGAGGAACGCCCGATGATCGCCTTGATTGGGGCCCGTCTCTTATTTGCAGCGACGACACAAAATATTCTGATGGCAAGGGCAACCCTGACACTGGAGCTGACTTTGGTGGTGCTCCTGACATTGATCACAAAAACACAAGGGTCCAGAATGAGCTCTCGGATTGGATGAATTGGTTGAAGACCGAAATTGGGTTCAGCGGATGGAGATTTGATTTTGTCAAGGGATATGCTCCAGAGTTCACCAAGTTGTATGTAGCAAACACAAAGCCTAGTTTCTCAGTAGGAGAGTTATGGAATTCCCTTGCGTATGAAAGTGATGGGAAACCAAAGTACAACCAGGATAGCCATCGCAACGAGCTTGCTGGATGGATAAGGAATGCTGGAGGAGAGGTTACCGCATTTGATTTTACAACTAAAGGGATACTTCAAGCTGCTGTCCAAGGTGAACTGGGGAGGTTGAAGGACTCAAATGGAGGGCCTCCTGGATTGATTGGAATATCGCCACGCAATAGTGTGACTTTCATAGACAATCATGATACAGGTTCCACACAAAAGTTGTGGCCATTTCCATCAGATGAAGTCATTCAGGGATATGCTTACATTCTTACTCATCCAGGGATCCCATCGATATTCTATGATCATTTCTTTGACTGGAGATTGAAAGAGCAGATCTCGAATCTCACTGTGATTAGGTCCAGGAATGGGATCAAACCCGACAGTGTGGTGCGGATCCTAGCAGCTGATCCAGATGTCTATGTAGCTTCCATAGATGACAAAATCATAATGAAGATTGGTATGCGATTTGATGTTAGCGAATTTGTACCTCCAACAACATTCAAAATTGCAACTTCTGGAATGAGCTATTGTGTTTGGGAGAAAACAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

351

Amino Acids

39.2

Weight (kDa)

5.25

Isoelectric Point (pI)

25.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amyl_C2 PF07821 289 - 348 4.9e-21 Alpha-amylase C-terminal beta-sheet domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
AciI CCGC 3 cut(s) 366, 564, 895
AclWI GGATC 6 cut(s) 781, 794, 881, 892, 905, 905
AcoI YGGCCR 1 cut(s) 728
AcsI RAATTY 2 cut(s) 463, 983
AfaI GTAC 2 cut(s) 500, 990
AfiI CCNNNNNNNGG 4 cut(s) 196, 260, 357, 870
AgsI TTSAA 6 cut(s) 167, 346, 596, 628, 829, 1006
AjnI CCWGG 5 cut(s) 4, 504, 649, 781, 863
AleI CACNNNNGTG 1 cut(s) 123
AluBI AGCT 7 cut(s) 266, 322, 526, 599, 908, 929, 1029
AluI AGCT 7 cut(s) 266, 322, 526, 599, 908, 929, 1029
Alw21I GWGCWC 2 cut(s) 283, 324
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 6 cut(s) 781, 794, 881, 892, 905, 905
AoxI GGCC 3 cut(s) 199, 644, 728
ApaI GGGCCC 1 cut(s) 203
ApeKI GCWGC 3 cut(s) 216, 599, 905
ApoI RAATTY 2 cut(s) 463, 983
Asp700I GAANNNNTTC 1 cut(s) 750
AspS9I GGNCC 5 cut(s) 199, 200, 310, 644, 861
AsuHPI GGTGA 2 cut(s) 400, 623
AvaII GGWCC 2 cut(s) 310, 861
BaeGI GKGCMC 1 cut(s) 203
BalI TGGCCA 1 cut(s) 730
BamHI GGATCC 2 cut(s) 786, 897
BanII GRGCYC 2 cut(s) 203, 324
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 2 cut(s) 283, 324
BbvI GCAGC 3 cut(s) 228, 586, 917
BccI CCATC 7 cut(s) 233, 363, 479, 522, 528, 745, 799
BciT130I CCWGG 5 cut(s) 6, 506, 651, 783, 865
BciVI GTATCC 2 cut(s) 81, 582
BclI TGATCA 2 cut(s) 292, 805
BcoDI GTCTC 1 cut(s) 210
BfaI CTAG 2 cut(s) 438, 902
BfuI GTATCC 2 cut(s) 81, 582
BglI GCCNNNNNGGC 1 cut(s) 10
BglII AGATCT 1 cut(s) 837
BisI GCNGC 3 cut(s) 217, 600, 906
BlsI GCNGC 3 cut(s) 218, 601, 907
Bme1390I CCNGG 5 cut(s) 6, 506, 651, 783, 865
Bme18I GGWCC 2 cut(s) 310, 861
BmgT120I GGNCC 5 cut(s) 199, 200, 310, 644, 861
BmiI GGNNCC 6 cut(s) 200, 201, 311, 712, 788, 899
BmrFI CCNGG 5 cut(s) 6, 506, 651, 783, 865
BmrI ACTGGG 1 cut(s) 626
BmsI GCATC 1 cut(s) 35
BmuI ACTGGG 1 cut(s) 626
BpiI GAAGAC 1 cut(s) 353
BplI GAGNNNNNCTC 2 cut(s) 824, 856
BpmI CTGGAG 4 cut(s) 282, 384, 570, 841
Bsa29I ATCGAT 1 cut(s) 794
BsaBI GATNNNNATC 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 607, 782
BsaXI ACNNNNNCTCC 2 cut(s) 543, 573
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 260, 357, 870
Bse1I ACTGG 4 cut(s) 66, 265, 621, 824
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 5 cut(s) 6, 506, 651, 783, 865
BseCI ATCGAT 1 cut(s) 794
BseDI CCNNGG 2 cut(s) 607, 782
BseGI GGATG 4 cut(s) 339, 374, 539, 778
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 4 cut(s) 196, 260, 357, 870
BseMII CTCAG 1 cut(s) 459
BseNI ACTGG 4 cut(s) 66, 265, 621, 824
BseRI GAGGAG 1 cut(s) 567
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 3 cut(s) 228, 586, 917
BsgI GTGCAG 1 cut(s) 147
BshFI GGCC 3 cut(s) 201, 646, 730
BshVI ATCGAT 1 cut(s) 794
BsiHKAI GWGCWC 2 cut(s) 283, 324
BslI CCNNNNNNNGG 4 cut(s) 196, 260, 357, 870
BsmAI GTCTC 1 cut(s) 210
BsmBI CGTCTC 1 cut(s) 210
BsmI GAATGC 1 cut(s) 550
BsnI GGCC 3 cut(s) 201, 646, 730
Bsp120I GGGCCC 1 cut(s) 199
Bsp1286I GDGCHC 3 cut(s) 203, 283, 324
Bsp143I GATC 9 cut(s) 19, 184, 292, 786, 805, 837, 873, 897, 910
BspACI CCGC 3 cut(s) 366, 564, 895
BspANI GGCC 3 cut(s) 201, 646, 730
BspCNI CTCAG 1 cut(s) 458
BspDI ATCGAT 1 cut(s) 794
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 6 cut(s) 200, 201, 311, 712, 788, 899
BspPI GGATC 6 cut(s) 781, 794, 881, 892, 905, 905
BsrI ACTGG 4 cut(s) 66, 265, 621, 824
BssECI CCNNGG 2 cut(s) 607, 782
BssMI GATC 9 cut(s) 19, 184, 292, 786, 805, 837, 873, 897, 910
BssT1I CCWWGG 1 cut(s) 607
Bst2UI CCWGG 5 cut(s) 6, 506, 651, 783, 865
Bst4CI ACNGT 2 cut(s) 853, 887
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 1 cut(s) 445
BstEII GGTNACC 1 cut(s) 559
BstF5I GGATG 4 cut(s) 339, 374, 539, 778
BstKTI GATC 9 cut(s) 22, 187, 295, 789, 808, 840, 876, 900, 913
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 9 cut(s) 19, 184, 292, 786, 805, 837, 873, 897, 910
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 5 cut(s) 6, 506, 651, 783, 865
BstPI GGTNACC 1 cut(s) 559
BstSCI CCNGG 5 cut(s) 4, 504, 649, 781, 863
BstSLI GKGCMC 1 cut(s) 203
BstV1I GCAGC 3 cut(s) 228, 586, 917
BstV2I GAAGAC 1 cut(s) 353
BstX2I RGATCY 3 cut(s) 786, 837, 897
BstXI CCANNNNNNTGG 1 cut(s) 83
BstYI RGATCY 3 cut(s) 786, 837, 897
Bsu15I ATCGAT 1 cut(s) 794
BsuI GTATCC 2 cut(s) 81, 582
BsuRI GGCC 3 cut(s) 201, 646, 730
BsuTUI ATCGAT 1 cut(s) 794
BtgZI GCGATG 1 cut(s) 500
BtsCI GGATG 4 cut(s) 339, 374, 539, 778
BtsIMutI CAGTG 4 cut(s) 59, 258, 849, 892
Cac8I GCNNGC 1 cut(s) 528
CciI TCATGA 1 cut(s) 700
Cfr13I GGNCC 5 cut(s) 199, 200, 310, 644, 861
ClaI ATCGAT 1 cut(s) 794
Csp6I GTAC 2 cut(s) 499, 989
CspCI CAANNNNNGTGG 2 cut(s) 707, 742
CviAII CATG 3 cut(s) 77, 701, 1053
CviQI GTAC 2 cut(s) 499, 989
DdeI CTNAG 1 cut(s) 445
DpnI GATC 9 cut(s) 21, 186, 294, 788, 807, 839, 875, 899, 912
DpnII GATC 9 cut(s) 19, 184, 292, 786, 805, 837, 873, 897, 910
EaeI YGGCCR 1 cut(s) 728
Ecl136II GAGCTC 1 cut(s) 322
Eco130I CCWWGG 1 cut(s) 607
Eco24I GRGCYC 2 cut(s) 203, 324
Eco47I GGWCC 2 cut(s) 310, 861
Eco53kI GAGCTC 1 cut(s) 322
Eco91I GGTNACC 1 cut(s) 559
EcoICRI GAGCTC 1 cut(s) 322
EcoO109I RGGNCCY 2 cut(s) 199, 644
EcoO65I GGTNACC 1 cut(s) 559
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 5 cut(s) 4, 504, 649, 781, 863
EcoT14I CCWWGG 1 cut(s) 607
EcoT38I GRGCYC 2 cut(s) 203, 324
ErhI CCWWGG 1 cut(s) 607
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 3 cut(s) 80, 704, 1056
FalI AAGNNNNNCTT 2 cut(s) 576, 608
FatI CATG 3 cut(s) 76, 700, 1052
FbaI TGATCA 2 cut(s) 292, 805
Fnu4HI GCNGC 3 cut(s) 217, 600, 906
FokI GGATG 4 cut(s) 346, 381, 546, 765
FriOI GRGCYC 2 cut(s) 203, 324
Fsp4HI GCNGC 3 cut(s) 217, 600, 906
FspBI CTAG 2 cut(s) 438, 902
GluI GCNGC 3 cut(s) 217, 600, 906
GsuI CTGGAG 4 cut(s) 282, 384, 570, 841
HaeIII GGCC 3 cut(s) 201, 646, 730
Hin1II CATG 3 cut(s) 80, 704, 1056
HinfI GANTC 2 cut(s) 632, 844
HphI GGTGA 2 cut(s) 400, 623
Hpy166II GTNNAC 2 cut(s) 408, 614
Hpy188I TCNGA 3 cut(s) 238, 328, 742
Hpy188III TCNNGA 7 cut(s) 284, 313, 401, 701, 841, 914, 1020
Hpy8I GTNNAC 2 cut(s) 408, 614
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 2 cut(s) 161, 622
HpyCH4III ACNGT 2 cut(s) 853, 887
HpyCH4V TGCA 4 cut(s) 128, 159, 216, 1013
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 445
Hsp92II CATG 3 cut(s) 80, 704, 1056
Ksp22I TGATCA 2 cut(s) 292, 805
Kzo9I GATC 9 cut(s) 19, 184, 292, 786, 805, 837, 873, 897, 910
LmnI GCTCC 3 cut(s) 263, 286, 403
Lsp1109I GCAGC 3 cut(s) 228, 586, 917
LweI GCATC 1 cut(s) 35
MaeI CTAG 2 cut(s) 438, 902
MaeIII GTNAC 2 cut(s) 559, 682
MalI GATC 9 cut(s) 21, 186, 294, 788, 807, 839, 875, 899, 912
MboI GATC 9 cut(s) 19, 184, 292, 786, 805, 837, 873, 897, 910
MboII GAAGA 3 cut(s) 145, 358, 967
MflI RGATCY 3 cut(s) 786, 837, 897
MhlI GDGCHC 3 cut(s) 203, 283, 324
MlsI TGGCCA 1 cut(s) 730
MluCI AATT 6 cut(s) 50, 337, 354, 463, 983, 1008
MluNI TGGCCA 1 cut(s) 730
MlyI GAGTC 1 cut(s) 626
MmeI TCCRAC 1 cut(s) 1019
MnlI CCTC 8 cut(s) 140, 164, 545, 550, 615, 635, 657, 1002
Mox20I TGGCCA 1 cut(s) 730
MroXI GAANNNNTTC 1 cut(s) 750
MscI TGGCCA 1 cut(s) 730
MseI TTAA 1 cut(s) 53
MslI CAYNNNNRTG 2 cut(s) 81, 123
Msp20I TGGCCA 1 cut(s) 730
MspA1I CMGCKG 2 cut(s) 366, 908
MspR9I CCNGG 5 cut(s) 6, 506, 651, 783, 865
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 5 cut(s) 6, 506, 651, 783, 865
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 9 cut(s) 19, 184, 292, 786, 805, 837, 873, 897, 910
NlaIII CATG 3 cut(s) 80, 704, 1056
NlaIV GGNNCC 6 cut(s) 200, 201, 311, 712, 788, 899
NmuCI GTSAC 1 cut(s) 682
OliI CACNNNNGTG 1 cut(s) 123
PagI TCATGA 1 cut(s) 700
PctI GAATGC 1 cut(s) 550
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 1 cut(s) 844
PfoI TCCNGGA 2 cut(s) 649, 863
PkrI GCNGC 3 cut(s) 218, 601, 907
PleI GAGTC 1 cut(s) 626
PpsI GAGTC 1 cut(s) 626
PsiI TTATAA 1 cut(s) 113
Psp124BI GAGCTC 1 cut(s) 324
Psp6I CCWGG 5 cut(s) 4, 504, 649, 781, 863
PspEI GGTNACC 1 cut(s) 559
PspGI CCWGG 5 cut(s) 4, 504, 649, 781, 863
PspN4I GGNNCC 6 cut(s) 200, 201, 311, 712, 788, 899
PspOMI GGGCCC 1 cut(s) 199
PspPI GGNCC 5 cut(s) 199, 200, 310, 644, 861
PsuI RGATCY 3 cut(s) 786, 837, 897
PvuII CAGCTG 1 cut(s) 908
RsaI GTAC 2 cut(s) 500, 990
RsaNI GTAC 2 cut(s) 499, 989
RseI CAYNNNNRTG 2 cut(s) 81, 123
SacI GAGCTC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 53
SatI GCNGC 3 cut(s) 217, 600, 906
Sau3AI GATC 9 cut(s) 19, 184, 292, 786, 805, 837, 873, 897, 910
Sau96I GGNCC 5 cut(s) 199, 200, 310, 644, 861
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 5 cut(s) 6, 506, 651, 783, 865
SduI GDGCHC 3 cut(s) 203, 283, 324
SfaNI GCATC 1 cut(s) 35
SinI GGWCC 2 cut(s) 310, 861
SmiMI CAYNNNNRTG 2 cut(s) 81, 123
Sse9I AATT 6 cut(s) 50, 337, 354, 463, 983, 1008
SsiI CCGC 3 cut(s) 366, 564, 895
SspI AATATT 1 cut(s) 233
SspMI CTAG 2 cut(s) 438, 902
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 5 cut(s) 4, 504, 649, 781, 863
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 2 cut(s) 853, 887
TaqI TCGA 2 cut(s) 794, 842
TaqII GACCGA 1 cut(s) 365
TasI AATT 6 cut(s) 50, 337, 354, 463, 983, 1008
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 1 cut(s) 844
Tru1I TTAA 1 cut(s) 53
Tru9I TTAA 1 cut(s) 53
TscAI CASTG 4 cut(s) 66, 265, 856, 892
TseFI GTSAC 1 cut(s) 682
TseI GCWGC 3 cut(s) 216, 599, 905
Tsp45I GTSAC 1 cut(s) 682
TspDTI ATGAA 6 cut(s) 63, 350, 492, 679, 759, 968
TspRI CASTG 4 cut(s) 66, 265, 856, 892
VpaK11BI GGWCC 2 cut(s) 310, 861
XapI RAATTY 2 cut(s) 463, 983
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 2 cut(s) 438, 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.