FvH4_2g06030

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001929
Physical Location & Seq
Forward (+)
5029481 .. 5035827
6347 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06030.t9

Sequence Viewer

Length: 1386 bp
ATGACTGCTCAATCTCAGGTGTTGATTAGTTACCAAAACAGTGAAAACGGACGCCTATATATAATAAGCTCTAGAGTAGAGGTTTGCTTCATCACAAAGCAGAGGAGAGGCATGAATTCACTTAAAATTTCCACCCTTTGTTTCCTACTCTCTATCATTTCCATACCCAACTTCACTGTTTCCCAAATCCTATTTCAGGGGTTCAATTGGGAATCAGCAAAGAAGGGAGGATGGTACAACATCTTGGAGCAGTCCGCAGCTGAACTAGCTTCTTCAGGAATTACACATGTTTGGCTTCCACCACCTTCTCAGGCTATTTCAGATGAAGGCTACATGCCTGGCAGGCTCTATGATCTCACATCAAAATATGGAAACCAAGATGGATTAAAGGCACTGGTAAAAGCCTACCAAGACAATGGGATAAAGAGCGTAGCAGATATTGTTATAAACCATAGATGTGCAGAGAAGCAAGATGAAAGAGGAATATGGTGCATTTTTGAGGGAGGAACACCAGATGATCGCCTTGATTGGGGGCCGTCTCTTATTTGCAGTGACGACACAAAATATTCTGATGGCAAGGGAAACCCTGATACTGGAGCTGACTTTGGTGGTGCTCCTGACATTGATCACAAAAACACAAGGGTCCAGAGTGAGCTCTCGGATTGGATGAACTGGTTAAAGACCGAAATTGGTTTCAGCGGCTGGCGATTTGATTTTGTCAGGGGATATGCTCCAGAGTTCACCAAGCTGTATGTGGCCAACACAAAGCCTAGTTTCTCAGTAGGAGAGTATTGGAATTCCATTGCTTATGGAAGTGATGGAAAACCTGAGTACAACCAGGACAACCATCGCAACGAGCTCGCTGGATGGATAGGGAATGCTGGAGGAGAGGTTACTGCTTTTGATTTTACAACAAAAGGGATACTTCAAGCTGCTGTCCAGGGTGAACTGTGGAGGTTGAAGGACTCGAATGGAGGTCCTCCTGGATTGATTGGAATATCGCCACGCAATAGTGTGACATTCATAGACAATCATGATACAGGTTCCACACAAAAATTGTGGCCATTTCCATCAGATAAAGTCATGCAGGGATATGCTTACACTCTTACTCATCCAGGCATCCCATCAATATTCTATGACCACTTCTTTGACTGGGGATTGAAAGGGGAGATCTCGAACCTCACTGCAATTAGGTCCAGGAATGGGATCAAGCCCGACAGTGCCGTCAGTATCCTAGCAGCTGATGCAGATCTCTATGTAGCTTCCATAGATGACAAAATCATAACCAAGATTGGTCCAAGATTCGATGTTGGGAACCTTGTACCGTCAACATTCAAAATTGCAACTTCTGGAAAGGATTATTGTGTTTGGGAGAAATCAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

462

Amino Acids

51.01

Weight (kDa)

5.39

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amylase PF00128 91 - 156 1.7e-08 Alpha amylase, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
AasI GACNNNNNNGTC 1 cut(s) 1223
AciI CCGC 2 cut(s) 255, 699
AclWI GGATC 1 cut(s) 1214
AcoI YGGCCR 2 cut(s) 756, 1061
AcsI RAATTY 3 cut(s) 115, 126, 796
AcuI CTGAAG 1 cut(s) 258
AcyI GRCGYC 1 cut(s) 52
AfaI GTAC 3 cut(s) 236, 833, 1323
AfiI CCNNNNNNNGG 4 cut(s) 196, 529, 593, 1203
AflIII ACRYGT 1 cut(s) 286
AgsI TTSAA 5 cut(s) 205, 929, 961, 1162, 1336
AjnI CCWGG 6 cut(s) 337, 837, 939, 982, 1114, 1196
AjuI GAANNNNNNNTTGG 2 cut(s) 177, 209
Alw21I GWGCWC 3 cut(s) 616, 657, 861
Alw26I GTCTC 1 cut(s) 543
AlwI GGATC 1 cut(s) 1214
AlwNI CAGNNNCTG 1 cut(s) 702
AoxI GGCC 3 cut(s) 533, 756, 1061
ApeKI GCWGC 3 cut(s) 257, 932, 1238
ApoI RAATTY 3 cut(s) 115, 126, 796
ArsI GACNNNNNNTTYG 2 cut(s) 1130, 1162
AspS9I GGNCC 5 cut(s) 533, 643, 977, 1194, 1295
AsuHPI GGTGA 2 cut(s) 733, 956
AvaII GGWCC 4 cut(s) 643, 977, 1194, 1295
BalI TGGCCA 2 cut(s) 758, 1063
BanII GRGCYC 2 cut(s) 657, 861
Bbv12I GWGCWC 3 cut(s) 616, 657, 861
BbvI GCAGC 3 cut(s) 269, 919, 1250
BccI CCATC 8 cut(s) 225, 374, 566, 812, 855, 861, 1078, 1132
BceAI ACGGC 2 cut(s) 520, 1208
BcgI CGANNNNNNTGC 2 cut(s) 841, 875
BciT130I CCWGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
BciVI GTATCC 2 cut(s) 915, 1241
BclI TGATCA 1 cut(s) 625
BcoDI GTCTC 1 cut(s) 543
BfaI CTAG 4 cut(s) 72, 266, 771, 1235
BfuI GTATCC 2 cut(s) 915, 1241
BglI GCCNNNNNGGC 1 cut(s) 343
BglII AGATCT 2 cut(s) 1170, 1249
BisI GCNGC 4 cut(s) 258, 700, 933, 1239
BlsI GCNGC 4 cut(s) 259, 701, 934, 1240
Bme1390I CCNGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
Bme18I GGWCC 4 cut(s) 643, 977, 1194, 1295
BmgT120I GGNCC 5 cut(s) 533, 643, 977, 1194, 1295
BmiI GGNNCC 4 cut(s) 534, 644, 1045, 1316
BmrFI CCNGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
BmrI ACTGGG 1 cut(s) 1162
BmsI GCATC 2 cut(s) 1128, 1234
BmuI ACTGGG 1 cut(s) 1162
BpmI CTGGAG 3 cut(s) 615, 717, 903
BsaBI GATNNNNATC 1 cut(s) 1248
BsaHI GRCGYC 1 cut(s) 52
BsaJI CCNNGG 1 cut(s) 940
BsaXI ACNNNNNCTCC 2 cut(s) 876, 906
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 529, 593, 1203
Bse1I ACTGG 4 cut(s) 399, 598, 677, 1157
Bse3DI GCAATG 1 cut(s) 801
Bse8I GATNNNNATC 1 cut(s) 1248
BseBI CCWGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
BseDI CCNNGG 1 cut(s) 940
BseGI GGATG 5 cut(s) 236, 672, 872, 1111, 1119
BseJI GATNNNNATC 1 cut(s) 1248
BseLI CCNNNNNNNGG 4 cut(s) 196, 529, 593, 1203
BseMI GCAATG 1 cut(s) 801
BseMII CTCAG 4 cut(s) 29, 323, 792, 819
BseNI ACTGG 4 cut(s) 399, 598, 677, 1157
BseRI GAGGAG 2 cut(s) 118, 900
BseXI GCAGC 3 cut(s) 269, 919, 1250
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 3 cut(s) 535, 758, 1063
BsiHKAI GWGCWC 3 cut(s) 616, 657, 861
BslI CCNNNNNNNGG 4 cut(s) 196, 529, 593, 1203
BsmAI GTCTC 1 cut(s) 543
BsmBI CGTCTC 1 cut(s) 543
BsmI GAATGC 1 cut(s) 883
BsnI GGCC 3 cut(s) 535, 758, 1063
Bsp1286I GDGCHC 3 cut(s) 616, 657, 861
Bsp143I GATC 6 cut(s) 352, 517, 625, 1170, 1206, 1249
BspACI CCGC 2 cut(s) 255, 699
BspANI GGCC 3 cut(s) 535, 758, 1063
BspCNI CTCAG 4 cut(s) 28, 322, 791, 820
BspHI TCATGA 1 cut(s) 1033
BspLI GGNNCC 4 cut(s) 534, 644, 1045, 1316
BspPI GGATC 1 cut(s) 1214
BsrDI GCAATG 1 cut(s) 801
BsrI ACTGG 4 cut(s) 399, 598, 677, 1157
BssECI CCNNGG 1 cut(s) 940
BssMI GATC 6 cut(s) 352, 517, 625, 1170, 1206, 1249
BssNI GRCGYC 1 cut(s) 52
Bst2UI CCWGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
Bst4CI ACNGT 5 cut(s) 41, 178, 951, 1220, 1326
BstACI GRCGYC 1 cut(s) 52
BstAPI GCANNNNNTGC 1 cut(s) 1244
BstC8I GCNNGC 3 cut(s) 344, 704, 861
BstDEI CTNAG 4 cut(s) 15, 309, 778, 828
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 5 cut(s) 236, 672, 872, 1111, 1119
BstKTI GATC 6 cut(s) 355, 520, 628, 1173, 1209, 1252
BstMAI GTCTC 1 cut(s) 543
BstMBI GATC 6 cut(s) 352, 517, 625, 1170, 1206, 1249
BstMWI GCNNNNNNNGC 3 cut(s) 266, 343, 1244
BstNI CCWGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
BstNSI RCATGY 2 cut(s) 290, 337
BstSCI CCNGG 6 cut(s) 337, 837, 939, 982, 1114, 1196
BstV1I GCAGC 3 cut(s) 269, 919, 1250
BstX2I RGATCY 2 cut(s) 1170, 1249
BstXI CCANNNNNNTGG 1 cut(s) 416
BstYI RGATCY 2 cut(s) 1170, 1249
BsuI GTATCC 2 cut(s) 915, 1241
BsuRI GGCC 3 cut(s) 535, 758, 1063
BtgZI GCGATG 1 cut(s) 833
BtsCI GGATG 5 cut(s) 236, 672, 872, 1111, 1119
BtsI GCAGTG 2 cut(s) 556, 1182
BtsIMutI CAGTG 6 cut(s) 46, 174, 392, 556, 1182, 1225
Cac8I GCNNGC 3 cut(s) 344, 704, 861
CaiI CAGNNNCTG 1 cut(s) 702
CciI TCATGA 1 cut(s) 1033
Cfr13I GGNCC 5 cut(s) 533, 643, 977, 1194, 1295
CseI GACGC 1 cut(s) 60
Csp6I GTAC 3 cut(s) 235, 832, 1322
CspCI CAANNNNNGTGG 2 cut(s) 1040, 1075
CviAII CATG 6 cut(s) 112, 287, 334, 1034, 1084, 1383
CviQI GTAC 3 cut(s) 235, 832, 1322
DdeI CTNAG 4 cut(s) 15, 309, 778, 828
DpnI GATC 6 cut(s) 354, 519, 627, 1172, 1208, 1251
DpnII GATC 6 cut(s) 352, 517, 625, 1170, 1206, 1249
DrdI GACNNNNNNGTC 1 cut(s) 1223
DseDI GACNNNNNNGTC 1 cut(s) 1223
EaeI YGGCCR 2 cut(s) 756, 1061
Ecl136II GAGCTC 2 cut(s) 655, 859
Eco24I GRGCYC 2 cut(s) 657, 861
Eco47I GGWCC 4 cut(s) 643, 977, 1194, 1295
Eco53kI GAGCTC 2 cut(s) 655, 859
Eco57I CTGAAG 1 cut(s) 258
EcoICRI GAGCTC 2 cut(s) 655, 859
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 977
EcoRI GAATTC 2 cut(s) 115, 796
EcoRII CCWGG 6 cut(s) 337, 837, 939, 982, 1114, 1196
EcoT38I GRGCYC 2 cut(s) 657, 861
Esp3I CGTCTC 1 cut(s) 543
FaeI CATG 6 cut(s) 115, 290, 337, 1037, 1087, 1386
FalI AAGNNNNNCTT 2 cut(s) 909, 941
FatI CATG 6 cut(s) 111, 286, 333, 1033, 1083, 1382
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 4 cut(s) 258, 700, 933, 1239
FokI GGATG 5 cut(s) 243, 679, 879, 1098, 1106
FriOI GRGCYC 2 cut(s) 657, 861
Fsp4HI GCNGC 4 cut(s) 258, 700, 933, 1239
FspBI CTAG 4 cut(s) 72, 266, 771, 1235
GluI GCNGC 4 cut(s) 258, 700, 933, 1239
GsuI CTGGAG 3 cut(s) 615, 717, 903
HaeIII GGCC 3 cut(s) 535, 758, 1063
HgaI GACGC 1 cut(s) 60
Hin1I GRCGYC 1 cut(s) 52
Hin1II CATG 6 cut(s) 115, 290, 337, 1037, 1087, 1386
HincII GTYRAC 1 cut(s) 1329
HindII GTYRAC 1 cut(s) 1329
HinfI GANTC 3 cut(s) 212, 965, 1302
HphI GGTGA 2 cut(s) 733, 956
Hpy166II GTNNAC 3 cut(s) 741, 947, 1329
Hpy188I TCNGA 4 cut(s) 322, 571, 661, 1075
Hpy188III TCNNGA 8 cut(s) 72, 276, 617, 646, 734, 1034, 1174, 1350
Hpy8I GTNNAC 3 cut(s) 741, 947, 1329
HpyAV CCTTC 4 cut(s) 217, 315, 320, 955
HpyCH4III ACNGT 5 cut(s) 41, 178, 951, 1220, 1326
HpyCH4V TGCA 7 cut(s) 461, 492, 549, 1087, 1187, 1247, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 343, 1244
HpyF3I CTNAG 4 cut(s) 15, 309, 778, 828
Hsp92I GRCGYC 1 cut(s) 52
Hsp92II CATG 6 cut(s) 115, 290, 337, 1037, 1087, 1386
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 6 cut(s) 352, 517, 625, 1170, 1206, 1249
LmnI GCTCC 4 cut(s) 247, 596, 619, 736
Lsp1109I GCAGC 3 cut(s) 269, 919, 1250
LweI GCATC 2 cut(s) 1128, 1234
MaeI CTAG 4 cut(s) 72, 266, 771, 1235
MaeIII GTNAC 4 cut(s) 29, 551, 892, 1015
MalI GATC 6 cut(s) 354, 519, 627, 1172, 1208, 1251
MboI GATC 6 cut(s) 352, 517, 625, 1170, 1206, 1249
MboII GAAGA 1 cut(s) 264
MfeI CAATTG 1 cut(s) 205
MflI RGATCY 2 cut(s) 1170, 1249
MhlI GDGCHC 3 cut(s) 616, 657, 861
MlsI TGGCCA 2 cut(s) 758, 1063
MluCI AATT 9 cut(s) 115, 126, 205, 279, 687, 796, 1055, 1188, 1338
MluNI TGGCCA 2 cut(s) 758, 1063
MlyI GAGTC 1 cut(s) 959
Mox20I TGGCCA 2 cut(s) 758, 1063
MscI TGGCCA 2 cut(s) 758, 1063
MseI TTAA 3 cut(s) 123, 386, 677
MslI CAYNNNNRTG 1 cut(s) 456
Msp20I TGGCCA 2 cut(s) 758, 1063
MspA1I CMGCKG 3 cut(s) 260, 699, 1241
MspR9I CCNGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
MunI CAATTG 1 cut(s) 205
Mva1269I GAATGC 1 cut(s) 883
MvaI CCWGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
MwoI GCNNNNNNNGC 3 cut(s) 266, 343, 1244
NdeII GATC 6 cut(s) 352, 517, 625, 1170, 1206, 1249
NlaIII CATG 6 cut(s) 115, 290, 337, 1037, 1087, 1386
NlaIV GGNNCC 4 cut(s) 534, 644, 1045, 1316
NmuCI GTSAC 2 cut(s) 551, 1015
NspI RCATGY 2 cut(s) 290, 337
PagI TCATGA 1 cut(s) 1033
PciI ACATGT 1 cut(s) 286
PctI GAATGC 1 cut(s) 883
PfeI GAWTC 2 cut(s) 212, 1302
PfoI TCCNGGA 2 cut(s) 982, 1196
PkrI GCNGC 4 cut(s) 259, 701, 934, 1240
PleI GAGTC 1 cut(s) 959
PpsI GAGTC 1 cut(s) 959
PpuMI RGGWCCY 1 cut(s) 977
PscI ACATGT 1 cut(s) 286
PsiI TTATAA 1 cut(s) 446
Psp124BI GAGCTC 2 cut(s) 657, 861
Psp5II RGGWCCY 1 cut(s) 977
Psp6I CCWGG 6 cut(s) 337, 837, 939, 982, 1114, 1196
PspGI CCWGG 6 cut(s) 337, 837, 939, 982, 1114, 1196
PspN4I GGNNCC 4 cut(s) 534, 644, 1045, 1316
PspPI GGNCC 5 cut(s) 533, 643, 977, 1194, 1295
PspPPI RGGWCCY 1 cut(s) 977
PstNI CAGNNNCTG 1 cut(s) 702
PsuI RGATCY 2 cut(s) 1170, 1249
PvuII CAGCTG 2 cut(s) 260, 1241
RsaI GTAC 3 cut(s) 236, 833, 1323
RsaNI GTAC 3 cut(s) 235, 832, 1322
RseI CAYNNNNRTG 1 cut(s) 456
SacI GAGCTC 2 cut(s) 657, 861
SaqAI TTAA 3 cut(s) 123, 386, 677
SatI GCNGC 4 cut(s) 258, 700, 933, 1239
Sau3AI GATC 6 cut(s) 352, 517, 625, 1170, 1206, 1249
Sau96I GGNCC 5 cut(s) 533, 643, 977, 1194, 1295
SchI GAGTC 1 cut(s) 959
ScrFI CCNGG 6 cut(s) 339, 839, 941, 984, 1116, 1198
SduI GDGCHC 3 cut(s) 616, 657, 861
SfaNI GCATC 2 cut(s) 1128, 1234
SinI GGWCC 4 cut(s) 643, 977, 1194, 1295
SmiMI CAYNNNNRTG 1 cut(s) 456
Sse9I AATT 9 cut(s) 115, 126, 205, 279, 687, 796, 1055, 1188, 1338
SsiI CCGC 2 cut(s) 255, 699
SspI AATATT 2 cut(s) 566, 1131
SspMI CTAG 4 cut(s) 72, 266, 771, 1235
SstI GAGCTC 2 cut(s) 657, 861
StyD4I CCNGG 6 cut(s) 337, 837, 939, 982, 1114, 1196
TaaI ACNGT 5 cut(s) 41, 178, 951, 1220, 1326
TaqI TCGA 3 cut(s) 968, 1175, 1305
TaqII GACCGA 1 cut(s) 698
TasI AATT 9 cut(s) 115, 126, 205, 279, 687, 796, 1055, 1188, 1338
TatI WGTACW 1 cut(s) 831
TauI GCSGC 1 cut(s) 702
TfiI GAWTC 2 cut(s) 212, 1302
Tru1I TTAA 3 cut(s) 123, 386, 677
Tru9I TTAA 3 cut(s) 123, 386, 677
TscAI CASTG 6 cut(s) 46, 181, 399, 556, 1189, 1225
TseFI GTSAC 2 cut(s) 551, 1015
TseI GCWGC 3 cut(s) 257, 932, 1238
Tsp45I GTSAC 2 cut(s) 551, 1015
TspDTI ATGAA 6 cut(s) 79, 128, 339, 489, 683, 1012
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 6 cut(s) 46, 181, 399, 556, 1189, 1225
VpaK11BI GGWCC 4 cut(s) 643, 977, 1194, 1295
XagI CCTNNNNNAGG 1 cut(s) 194
XapI RAATTY 3 cut(s) 115, 126, 796
XbaI TCTAGA 1 cut(s) 71
XceI RCATGY 2 cut(s) 290, 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 751
XspI CTAG 4 cut(s) 72, 266, 771, 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.