Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0001929

Physical Location & Seq

Genomic Coordinates Reverse (-)

56290930 .. 56293163

Gene Structure 2234 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000014685

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1278 bp

ATGAATTCACTAGAAGTTTCCCTCCTCTGTTTCCTACTCTCTATTATTTTCATACCCAACTTCACTGCTTCCCAAATCCTATTTCAGGGGTTCAATTGGGAATCAGCAAAGAAGGGAGGATGGTACAACATCTTGAAGCAGTCTGCAGCTGAACTAGCTTCTTCAGGAATTACACACGTTTGGCTTCCACCACCTTCTCATGCTATTTCAGATGAAGGATACATGCCTGGTAGGCTTTATGATCTCACATCAAAATATGGAAGTCAAGATGAATTAAAGGCACTGGTAAAAACCTACCACGACAATGGGATACAGAGCATAGCAGATATTGTTATAAACCACAGATGTGCAGAGAAGCAAGATGAGAGAGGAATATGGTGCATTTTTGAAGGAGGAACGCCCGATGATCGCCTTGATTGGGGCCCGTCTCTTATTTGCAGCGACGACACAAAATATTCTGATGGCAAGGGCAACCCTGACACTGGAGCTGACTTTGGTGGTGCTCCTGACATTGATCACAAAAACACAAGGGTCCAGATTGAGCTCTCGGATTGGATGAATTGGTTGAAGACCGAAATTGGGTTCAGCGGATGGAGATTTGATTTTGTCAGGGGATATGCTCCAGAGTTCACCAAGTTGTATGTAGCAAACACAAAGCCTAGTTTCTCAGTAGGAGAGTTATGGAATTCCCTTGCTTATGAAAGTGATGGGAAACCCAAGTACAACCAGGACAACCATCGCAACGAGCTTGCTGGATGGATAGGGAATGCTGGAGGAGAGGTTACCGCATTTGATTTTACAACTAAAGGGATACTTCAAGCTGCTGTCCTAGGCGAACTGGGGAGGTTGAAGGACTCGAATGGAGGGCCTCCTGGATTGATTGGAATATCGCCACGCAATAGTGTAACTTTCATAGACAATCATGATACAGGTTCCACACAAAAGTTGTGGCCATTTCCATCAGATGAAGTCATTCAGGGATATGCTTACATTCTTACTCATCCAGGGATCCCATCGATATTCTATGATCATTTCTTTGACTGGAGATTGAAAGAGCAGATCTCGAATCTCACTGCGATTAGGTCCAGGAATGGGATCAAACCCGACAGTGTGGTGCGTATCCTAGCAGCTGATCCAGATGTCTATGTAGCTTCCATAGATGACAAAATCATAATGAAGATTGGTATGCGATTTGATGTTAGCGAATTTGTACCTCCAACAACATTCAAAATTGCAACTTCTGGAATGAGCTATTGTGTTTGGGAGAAAACAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 ko04973 map00500 map01100 map04973

Pfam Domains

Protein Families

Alpha-amyl_C2 Alpha-amylase

Protein Analysis

426

Amino Acids

47.31

Weight (kDa)

5.12

Isoelectric Point (pI)

31.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Alpha-amylase	PF00128	54 - 119	1.3e-08	Alpha amylase, catalytic domain
Alpha-amyl_C2	PF07821	363 - 422	6.6e-21	Alpha-amylase C-terminal beta-sheet domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001929)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G25000
fragaria_vesca	FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030 FvH4_2g06030
malus_domestica	MD00G1035200.v1.1 MD05G1081100.v1.1 MD05G1081200.v1.1
prunus_persica	Prupe.8G125500_v2.0.a1
pyrus_communis	pycom05g07460 pycom10g07850
rosa_chinensis	RchiOBHm_Chr6g0257251 RchiOBHm_Chr6g0257281 RchiOBHm_Chr6g0257311
rosa_laevigata	RLG00000014683 RLG00000014684 RLG00000014685 RLG00000014687
rosa_multiflora	Rmu_sc0000820.1_g000005 Rmu_sc0002648.1_g000009 Rmu_sc0008660.1_g000002
rosa_roxburghii	Rroxscaffold_7G00208570 Rroxscaffold_7G00208600 Rroxscaffold_7G00208610
rosa_rugosa	Rorug04G0263800 Rorug05G0573700 Rorug05G0573800 Rorug05G0574000
rosa_samantha	Rh6AG090900 Rh6AG091400 Rh6AG091700 Rh6BG082600 Rh6BG082700 Rh6CG078700 Rh6CG079000 Rh6CG079200 Rh6DG074500 Rh6DG074800
rosa_wichuraiana	Rw6G007930 Rw6G007940 Rw6G007950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	335
AciI	CCGC	2 cut(s)	588, 786
AclWI	GGATC	4 cut(s)	1003, 1016, 1103, 1127
AcoI	YGGCCR	1 cut(s)	950
AcsI	RAATTY	3 cut(s)	4, 685, 1205
AcuI	CTGAAG	1 cut(s)	147
AfaI	GTAC	3 cut(s)	125, 722, 1212
AfiI	CCNNNNNNNGG	5 cut(s)	85, 418, 482, 579, 1092
AflIII	ACRYGT	1 cut(s)	175
AgsI	TTSAA	8 cut(s)	94, 136, 389, 568, 818, 850, 1051, 1228
AjnI	CCWGG	5 cut(s)	226, 726, 871, 1003, 1085
AjuI	GAANNNNNNNTTGG	2 cut(s)	66, 98
AleI	CACNNNNGTG	1 cut(s)	345
AluBI	AGCT	9 cut(s)	149, 158, 488, 544, 748, 821, 1130, 1151, 1251
AluI	AGCT	9 cut(s)	149, 158, 488, 544, 748, 821, 1130, 1151, 1251
Alw21I	GWGCWC	2 cut(s)	505, 546
Alw26I	GTCTC	1 cut(s)	432
AlwI	GGATC	4 cut(s)	1003, 1016, 1103, 1127
AoxI	GGCC	3 cut(s)	421, 866, 950
ApaI	GGGCCC	1 cut(s)	425
ApeKI	GCWGC	4 cut(s)	146, 438, 821, 1127
ApoI	RAATTY	3 cut(s)	4, 685, 1205
Asp700I	GAANNNNTTC	1 cut(s)	972
AspA2I	CCTAGG	1 cut(s)	829
AspS9I	GGNCC	5 cut(s)	421, 422, 532, 866, 1083
AsuHPI	GGTGA	1 cut(s)	622
AvaII	GGWCC	2 cut(s)	532, 1083
AvrII	CCTAGG	1 cut(s)	829
BaeGI	GKGCMC	1 cut(s)	425
BalI	TGGCCA	1 cut(s)	952
BamHI	GGATCC	1 cut(s)	1008
BanII	GRGCYC	2 cut(s)	425, 546
BbsI	GAAGAC	1 cut(s)	575
Bbv12I	GWGCWC	2 cut(s)	505, 546
BbvI	GCAGC	4 cut(s)	158, 450, 808, 1139
BccI	CCATC	8 cut(s)	114, 455, 585, 701, 744, 750, 967, 1021
BciT130I	CCWGG	5 cut(s)	228, 728, 873, 1005, 1087
BciVI	GTATCC	4 cut(s)	212, 303, 804, 1130
BclI	TGATCA	2 cut(s)	514, 1027
BcoDI	GTCTC	1 cut(s)	432
BfaI	CTAG	5 cut(s)	11, 155, 660, 830, 1124
BfmI	CTRYAG	1 cut(s)	144
BfuI	GTATCC	4 cut(s)	212, 303, 804, 1130
BglI	GCCNNNNNGGC	1 cut(s)	232
BglII	AGATCT	1 cut(s)	1059
BisI	GCNGC	4 cut(s)	147, 439, 822, 1128
BlnI	CCTAGG	1 cut(s)	829
BlsI	GCNGC	4 cut(s)	148, 440, 823, 1129
Bme1390I	CCNGG	5 cut(s)	228, 728, 873, 1005, 1087
Bme18I	GGWCC	2 cut(s)	532, 1083
BmgT120I	GGNCC	5 cut(s)	421, 422, 532, 866, 1083
BmiI	GGNNCC	5 cut(s)	422, 423, 533, 934, 1010
BmrFI	CCNGG	5 cut(s)	228, 728, 873, 1005, 1087
BmrI	ACTGGG	1 cut(s)	848
BmuI	ACTGGG	1 cut(s)	848
BpiI	GAAGAC	1 cut(s)	575
BplI	GAGNNNNNCTC	2 cut(s)	1046, 1078
BpmI	CTGGAG	4 cut(s)	504, 606, 792, 1063
Bsa29I	ATCGAT	1 cut(s)	1016
BsaJI	CCNNGG	2 cut(s)	829, 1004
BsaXI	ACNNNNNCTCC	2 cut(s)	765, 795
Bsc4I	CCNNNNNNNGG	5 cut(s)	85, 418, 482, 579, 1092
Bse1I	ACTGG	4 cut(s)	288, 487, 843, 1046
BseBI	CCWGG	5 cut(s)	228, 728, 873, 1005, 1087
BseCI	ATCGAT	1 cut(s)	1016
BseDI	CCNNGG	2 cut(s)	829, 1004
BseGI	GGATG	5 cut(s)	125, 561, 596, 761, 1000
BseLI	CCNNNNNNNGG	5 cut(s)	85, 418, 482, 579, 1092
BseMII	CTCAG	1 cut(s)	681
BseNI	ACTGG	4 cut(s)	288, 487, 843, 1046
BseRI	GAGGAG	2 cut(s)	14, 789
BseSI	GKGCMC	1 cut(s)	425
BseXI	GCAGC	4 cut(s)	158, 450, 808, 1139
BsgI	GTGCAG	1 cut(s)	369
BshFI	GGCC	3 cut(s)	423, 868, 952
BshVI	ATCGAT	1 cut(s)	1016
BsiHKAI	GWGCWC	2 cut(s)	505, 546
BslI	CCNNNNNNNGG	5 cut(s)	85, 418, 482, 579, 1092
BsmAI	GTCTC	1 cut(s)	432
BsmBI	CGTCTC	1 cut(s)	432
BsmI	GAATGC	1 cut(s)	772
BsnI	GGCC	3 cut(s)	423, 868, 952
Bsp120I	GGGCCC	1 cut(s)	421
Bsp1286I	GDGCHC	3 cut(s)	425, 505, 546
Bsp143I	GATC	8 cut(s)	241, 406, 514, 1008, 1027, 1059, 1095, 1132
BspACI	CCGC	2 cut(s)	588, 786
BspANI	GGCC	3 cut(s)	423, 868, 952
BspCNI	CTCAG	1 cut(s)	680
BspDI	ATCGAT	1 cut(s)	1016
BspHI	TCATGA	1 cut(s)	922
BspLI	GGNNCC	5 cut(s)	422, 423, 533, 934, 1010
BspMAI	CTGCAG	1 cut(s)	148
BspPI	GGATC	4 cut(s)	1003, 1016, 1103, 1127
BsrI	ACTGG	4 cut(s)	288, 487, 843, 1046
BssECI	CCNNGG	2 cut(s)	829, 1004
BssMI	GATC	8 cut(s)	241, 406, 514, 1008, 1027, 1059, 1095, 1132
BssT1I	CCWWGG	1 cut(s)	829
Bst2UI	CCWGG	5 cut(s)	228, 728, 873, 1005, 1087
Bst4CI	ACNGT	1 cut(s)	1109
BstC8I	GCNNGC	1 cut(s)	750
BstDEI	CTNAG	1 cut(s)	667
BstEII	GGTNACC	1 cut(s)	781
BstENI	CCTNNNNNAGG	1 cut(s)	83
BstF5I	GGATG	5 cut(s)	125, 561, 596, 761, 1000
BstKTI	GATC	8 cut(s)	244, 409, 517, 1011, 1030, 1062, 1098, 1135
BstMAI	GTCTC	1 cut(s)	432
BstMBI	GATC	8 cut(s)	241, 406, 514, 1008, 1027, 1059, 1095, 1132
BstMWI	GCNNNNNNNGC	2 cut(s)	155, 232
BstNI	CCWGG	5 cut(s)	228, 728, 873, 1005, 1087
BstNSI	RCATGY	1 cut(s)	226
BstPI	GGTNACC	1 cut(s)	781
BstSCI	CCNGG	5 cut(s)	226, 726, 871, 1003, 1085
BstSFI	CTRYAG	1 cut(s)	144
BstSLI	GKGCMC	1 cut(s)	425
BstV1I	GCAGC	4 cut(s)	158, 450, 808, 1139
BstV2I	GAAGAC	1 cut(s)	575
BstX2I	RGATCY	2 cut(s)	1008, 1059
BstXI	CCANNNNNNTGG	1 cut(s)	305
BstYI	RGATCY	2 cut(s)	1008, 1059
Bsu15I	ATCGAT	1 cut(s)	1016
BsuI	GTATCC	4 cut(s)	212, 303, 804, 1130
BsuRI	GGCC	3 cut(s)	423, 868, 952
BsuTUI	ATCGAT	1 cut(s)	1016
BtgZI	GCGATG	1 cut(s)	722
BtsCI	GGATG	5 cut(s)	125, 561, 596, 761, 1000
BtsI	GCAGTG	2 cut(s)	63, 1071
BtsIMutI	CAGTG	5 cut(s)	63, 281, 480, 1071, 1114
Cac8I	GCNNGC	1 cut(s)	750
CciI	TCATGA	1 cut(s)	922
Cfr13I	GGNCC	5 cut(s)	421, 422, 532, 866, 1083
ClaI	ATCGAT	1 cut(s)	1016
Csp6I	GTAC	3 cut(s)	124, 721, 1211
CspCI	CAANNNNNGTGG	2 cut(s)	929, 964
CviAII	CATG	4 cut(s)	200, 223, 923, 1275
CviQI	GTAC	3 cut(s)	124, 721, 1211
DdeI	CTNAG	1 cut(s)	667
DpnI	GATC	8 cut(s)	243, 408, 516, 1010, 1029, 1061, 1097, 1134
DpnII	GATC	8 cut(s)	241, 406, 514, 1008, 1027, 1059, 1095, 1132
EaeI	YGGCCR	1 cut(s)	950
Ecl136II	GAGCTC	1 cut(s)	544
Eco130I	CCWWGG	1 cut(s)	829
Eco24I	GRGCYC	2 cut(s)	425, 546
Eco47I	GGWCC	2 cut(s)	532, 1083
Eco53kI	GAGCTC	1 cut(s)	544
Eco57I	CTGAAG	1 cut(s)	147
Eco91I	GGTNACC	1 cut(s)	781
EcoICRI	GAGCTC	1 cut(s)	544
EcoNI	CCTNNNNNAGG	1 cut(s)	83
EcoO109I	RGGNCCY	2 cut(s)	421, 866
EcoO65I	GGTNACC	1 cut(s)	781
EcoRI	GAATTC	2 cut(s)	4, 685
EcoRII	CCWGG	5 cut(s)	226, 726, 871, 1003, 1085
EcoT14I	CCWWGG	1 cut(s)	829
EcoT38I	GRGCYC	2 cut(s)	425, 546
ErhI	CCWWGG	1 cut(s)	829
Esp3I	CGTCTC	1 cut(s)	432
FaeI	CATG	4 cut(s)	203, 226, 926, 1278
FalI	AAGNNNNNCTT	2 cut(s)	798, 830
FatI	CATG	4 cut(s)	199, 222, 922, 1274
FbaI	TGATCA	2 cut(s)	514, 1027
Fnu4HI	GCNGC	4 cut(s)	147, 439, 822, 1128
FokI	GGATG	5 cut(s)	132, 568, 603, 768, 987
FriOI	GRGCYC	2 cut(s)	425, 546
Fsp4HI	GCNGC	4 cut(s)	147, 439, 822, 1128
FspBI	CTAG	5 cut(s)	11, 155, 660, 830, 1124
GluI	GCNGC	4 cut(s)	147, 439, 822, 1128
GsuI	CTGGAG	4 cut(s)	504, 606, 792, 1063
HaeIII	GGCC	3 cut(s)	423, 868, 952
Hin1II	CATG	4 cut(s)	203, 226, 926, 1278
HinfI	GANTC	3 cut(s)	101, 854, 1066
HphI	GGTGA	1 cut(s)	622
Hpy166II	GTNNAC	1 cut(s)	630
Hpy188I	TCNGA	4 cut(s)	211, 460, 550, 964
Hpy8I	GTNNAC	1 cut(s)	630
Hpy99I	CGWCG	1 cut(s)	446
HpyAV	CCTTC	5 cut(s)	106, 204, 209, 383, 844
HpyCH4III	ACNGT	1 cut(s)	1109
HpyCH4IV	ACGT	1 cut(s)	177
HpyCH4V	TGCA	5 cut(s)	146, 350, 381, 438, 1235
HpyF10VI	GCNNNNNNNGC	2 cut(s)	155, 232
HpyF3I	CTNAG	1 cut(s)	667
HpySE526I	ACGT	1 cut(s)	177
Hsp92II	CATG	4 cut(s)	203, 226, 926, 1278
Ksp22I	TGATCA	2 cut(s)	514, 1027
Kzo9I	GATC	8 cut(s)	241, 406, 514, 1008, 1027, 1059, 1095, 1132
LmnI	GCTCC	3 cut(s)	485, 508, 625
Lsp1109I	GCAGC	4 cut(s)	158, 450, 808, 1139
MaeI	CTAG	5 cut(s)	11, 155, 660, 830, 1124
MaeII	ACGT	1 cut(s)	177
MaeIII	GTNAC	2 cut(s)	781, 904
MalI	GATC	8 cut(s)	243, 408, 516, 1010, 1029, 1061, 1097, 1134
MboI	GATC	8 cut(s)	241, 406, 514, 1008, 1027, 1059, 1095, 1132
MboII	GAAGA	3 cut(s)	153, 580, 1189
MfeI	CAATTG	1 cut(s)	94
MflI	RGATCY	2 cut(s)	1008, 1059
MhlI	GDGCHC	3 cut(s)	425, 505, 546
MlsI	TGGCCA	1 cut(s)	952
MluCI	AATT	9 cut(s)	4, 94, 168, 272, 559, 576, 685, 1205, 1230
MluNI	TGGCCA	1 cut(s)	952
MlyI	GAGTC	1 cut(s)	848
MmeI	TCCRAC	1 cut(s)	1241
Mox20I	TGGCCA	1 cut(s)	952
MroXI	GAANNNNTTC	1 cut(s)	972
MscI	TGGCCA	1 cut(s)	952
MseI	TTAA	1 cut(s)	275
MslI	CAYNNNNRTG	2 cut(s)	303, 345
Msp20I	TGGCCA	1 cut(s)	952
MspA1I	CMGCKG	3 cut(s)	149, 588, 1130
MspR9I	CCNGG	5 cut(s)	228, 728, 873, 1005, 1087
MunI	CAATTG	1 cut(s)	94
Mva1269I	GAATGC	1 cut(s)	772
MvaI	CCWGG	5 cut(s)	228, 728, 873, 1005, 1087
MwoI	GCNNNNNNNGC	2 cut(s)	155, 232
NdeII	GATC	8 cut(s)	241, 406, 514, 1008, 1027, 1059, 1095, 1132
NlaIII	CATG	4 cut(s)	203, 226, 926, 1278
NlaIV	GGNNCC	5 cut(s)	422, 423, 533, 934, 1010
NspI	RCATGY	1 cut(s)	226
OliI	CACNNNNGTG	1 cut(s)	345
PagI	TCATGA	1 cut(s)	922
PctI	GAATGC	1 cut(s)	772
PdmI	GAANNNNTTC	1 cut(s)	972
PfeI	GAWTC	2 cut(s)	101, 1066
PfoI	TCCNGGA	2 cut(s)	871, 1085
PkrI	GCNGC	4 cut(s)	148, 440, 823, 1129
PleI	GAGTC	1 cut(s)	848
PpsI	GAGTC	1 cut(s)	848
PsiI	TTATAA	1 cut(s)	335
Psp124BI	GAGCTC	1 cut(s)	546
Psp6I	CCWGG	5 cut(s)	226, 726, 871, 1003, 1085
PspEI	GGTNACC	1 cut(s)	781
PspGI	CCWGG	5 cut(s)	226, 726, 871, 1003, 1085
PspN4I	GGNNCC	5 cut(s)	422, 423, 533, 934, 1010
PspOMI	GGGCCC	1 cut(s)	421
PspPI	GGNCC	5 cut(s)	421, 422, 532, 866, 1083
PstI	CTGCAG	1 cut(s)	148
PsuI	RGATCY	2 cut(s)	1008, 1059
PvuII	CAGCTG	2 cut(s)	149, 1130
RsaI	GTAC	3 cut(s)	125, 722, 1212
RsaNI	GTAC	3 cut(s)	124, 721, 1211
RseI	CAYNNNNRTG	2 cut(s)	303, 345
SacI	GAGCTC	1 cut(s)	546
SaqAI	TTAA	1 cut(s)	275
SatI	GCNGC	4 cut(s)	147, 439, 822, 1128
Sau3AI	GATC	8 cut(s)	241, 406, 514, 1008, 1027, 1059, 1095, 1132
Sau96I	GGNCC	5 cut(s)	421, 422, 532, 866, 1083
SchI	GAGTC	1 cut(s)	848
ScrFI	CCNGG	5 cut(s)	228, 728, 873, 1005, 1087
SduI	GDGCHC	3 cut(s)	425, 505, 546
SfcI	CTRYAG	1 cut(s)	144
SinI	GGWCC	2 cut(s)	532, 1083
SmiMI	CAYNNNNRTG	2 cut(s)	303, 345
Sse9I	AATT	9 cut(s)	4, 94, 168, 272, 559, 576, 685, 1205, 1230
SsiI	CCGC	2 cut(s)	588, 786
SspI	AATATT	1 cut(s)	455
SspMI	CTAG	5 cut(s)	11, 155, 660, 830, 1124
SstI	GAGCTC	1 cut(s)	546
StyD4I	CCNGG	5 cut(s)	226, 726, 871, 1003, 1085
StyI	CCWWGG	1 cut(s)	829
TaaI	ACNGT	1 cut(s)	1109
TaiI	ACGT	1 cut(s)	180
TaqI	TCGA	3 cut(s)	857, 1016, 1064
TaqII	GACCGA	1 cut(s)	587
TasI	AATT	9 cut(s)	4, 94, 168, 272, 559, 576, 685, 1205, 1230
TatI	WGTACW	1 cut(s)	720
TfiI	GAWTC	2 cut(s)	101, 1066
Tru1I	TTAA	1 cut(s)	275
Tru9I	TTAA	1 cut(s)	275
TscAI	CASTG	5 cut(s)	70, 288, 487, 1078, 1114
TseI	GCWGC	4 cut(s)	146, 438, 821, 1127
TspDTI	ATGAA	9 cut(s)	17, 40, 228, 285, 572, 714, 901, 981, 1190
TspRI	CASTG	5 cut(s)	70, 288, 487, 1078, 1114
VpaK11BI	GGWCC	2 cut(s)	532, 1083
XagI	CCTNNNNNAGG	1 cut(s)	83
XapI	RAATTY	3 cut(s)	4, 685, 1205
XceI	RCATGY	1 cut(s)	226
XmaJI	CCTAGG	1 cut(s)	829
XmnI	GAANNNNTTC	1 cut(s)	972
XspI	CTAG	5 cut(s)	11, 155, 660, 830, 1124

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.