MD16G1102400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011343
Physical Location & Seq
Forward (+)
7171093 .. 7173312
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1102400.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGGCCGTGGTTATCGGAAACCTAGCTCTGCTACTAGACGTCACGTCGCCGAGGACCATAATTCCTGATCGGAAGACTCGTCCGGTGGCGCTCGACGCGCTTTTGAACATGAACCTGACCTTGCCGAAGCGGGACCCGCAGAGTCACGCACACAACGGGTTTATCGCGGTCAAGGGGTTTGACTCGGACGGGGAGACTCGGAGCCAGCGAGTCGTGGCTCGGGGAAAAGCGAATTCGAAGGTAAACGGCGTGGACTTCGACAGGGACGAGGAGGGGAACGGGAATGGGTACGGGGACGGGGACGATCAGGAGCCGTTGGATTGGGAGAAGGAAATGCGGAAGAGGGTGAAGGAGATCGAGGAGAGGAGGGAGTTGGAGAAGAAGGCGGAGGAGATACAGAACCGGATGGAGGAGGATTTCGAAGACGACGGCAGGGAAGAGACGGAGCAGGAGAAGCGGATGAGAGTGAGGAAAGAGCTCGAAAAGGTGGCTAAGGAGCAGGCGGAGCGGAGAGCCACGGCGCAGTTGATGTTTGATTTGGGACAGAAGGCGTATGGAAGGGGCATGTACGGCCGTGCCATTGAGTTTTTAGAAGGCGCGCTCACGATCATTCCTAGGCCCACGTTATTCGGTGGTGAGATACAAATTTGGCTTGCTATGGCCTACGAGGCTAACAACCGCCATACCGATTGCATTGAACTTTACCATCAATTGGAGAAGAAGCACCCGAGTGCCAGCATCCGGCGCCAAGCAGCAGAGCTTCGGTACATTTTGCAAGCACCAAAGCTCAAGATAACACAAGAAGAGATGGTAACCATACCACTCATTGGTTCTAGTTATGACAGCTATGCTGGAACGTGGAGTGATAAATACAAAGGCAAAGAACCGACTAGCAGCGGGACAGTGACCAATCAGCTCTCGTCGTCTAGAGACTATTTCGGAGACTTTATGGTATGGCGACCTCCAATCGGACTGGAGAAAAACCAAGCGTTCTGGATAGGTTTGGCATTGTGGTTGGGTTTAGTTGGAGCTGCGCTCTTTATTCAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

39.82

Weight (kDa)

5.65

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26580
fragaria_vesca FvH4_4g25530 FvH4_4g25530
malus_domestica MD13G1101900.v1.1 MD16G1102400.v1.1
prunus_persica Prupe.1G243900_v2.0.a1 Prupe.1G243900_v2.0.a1
pyrus_communis pycom13g08900 pycom16g08700
rosa_chinensis RchiOBHm_Chr4g0432921
rosa_laevigata RLG00000006801
rosa_multiflora Rmu_sc0001762.1_g000047
rosa_roxburghii Rroxscaffold_5G00374330
rosa_rugosa Rorug04G0263800 Rorug04G0263900
rosa_samantha Rh4AG317900 Rh4BG326100 Rh4CG341200 Rh4DG321200
rosa_wichuraiana Rw4G027640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 744
AccB7I CCANNNNNTGG 2 cut(s) 712, 827
AccBSI CCGCTC 1 cut(s) 508
AccII CGCG 3 cut(s) 98, 167, 599
AcoI YGGCCR 2 cut(s) 3, 571
AcsI RAATTY 2 cut(s) 232, 645
AcyI GRCGYC 2 cut(s) 39, 745
AfaI GTAC 3 cut(s) 290, 569, 767
AfiI CCNNNNNNNGG 5 cut(s) 409, 712, 741, 827, 968
AgsI TTSAA 3 cut(s) 106, 698, 1046
AhdI GACNNNNNGTC 1 cut(s) 43
AjiI CACGTC 1 cut(s) 45
AleI CACNNNNGTG 1 cut(s) 729
AluBI AGCT 7 cut(s) 26, 478, 760, 787, 846, 916, 1031
AluI AGCT 7 cut(s) 26, 478, 760, 787, 846, 916, 1031
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 4 cut(s) 188, 434, 924, 936
Ama87I CYCGRG 2 cut(s) 219, 727
AoxI GGCC 4 cut(s) 3, 571, 617, 660
ApeKI GCWGC 3 cut(s) 752, 894, 1031
ApoI RAATTY 2 cut(s) 232, 645
AspA2I CCTAGG 1 cut(s) 614
AspLEI GCGC 7 cut(s) 91, 100, 523, 599, 601, 747, 1036
AspS9I GGNCC 3 cut(s) 54, 133, 618
AsuHPI GGTGA 2 cut(s) 358, 647
AsuII TTCGAA 2 cut(s) 236, 420
AvaI CYCGRG 2 cut(s) 219, 727
AvaII GGWCC 2 cut(s) 54, 133
AvrII CCTAGG 1 cut(s) 614
BanI GGYRCC 1 cut(s) 744
BanII GRGCYC 1 cut(s) 480
BbsI GAAGAC 2 cut(s) 80, 429
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 3 cut(s) 764, 906, 1018
BccI CCATC 3 cut(s) 400, 714, 802
BceAI ACGGC 6 cut(s) 262, 298, 445, 534, 558, 586
BcoDI GTCTC 4 cut(s) 188, 434, 924, 936
BfaI CTAG 6 cut(s) 23, 35, 615, 834, 891, 927
BfoI RGCGCY 2 cut(s) 92, 748
BglI GCCNNNNNGGC 1 cut(s) 668
BisI GCNGC 3 cut(s) 753, 895, 1032
BlnI CCTAGG 1 cut(s) 614
BlsI GCNGC 3 cut(s) 754, 896, 1033
Bme18I GGWCC 2 cut(s) 54, 133
BmeRI GACNNNNNGTC 1 cut(s) 43
BmeT110I CYCGRG 2 cut(s) 219, 727
BmgBI CACGTC 1 cut(s) 45
BmgT120I GGNCC 3 cut(s) 54, 133, 618
BmiI GGNNCC 5 cut(s) 134, 135, 203, 312, 746
BmsI GCATC 1 cut(s) 747
BpiI GAAGAC 2 cut(s) 80, 429
BplI GAGNNNNNCTC 2 cut(s) 1020, 1052
BpmI CTGGAG 1 cut(s) 995
Bpu10I CCTNAGC 1 cut(s) 492
Bpu14I TTCGAA 2 cut(s) 236, 420
BpuEI CTTGAG 1 cut(s) 773
BsaHI GRCGYC 2 cut(s) 39, 745
BsaJI CCNNGG 4 cut(s) 6, 50, 516, 614
BsaWI WCCGGW 2 cut(s) 82, 402
Bsc4I CCNNNNNNNGG 5 cut(s) 409, 712, 741, 827, 968
Bse1I ACTGG 1 cut(s) 978
BseDI CCNNGG 4 cut(s) 6, 50, 516, 614
BseGI GGATG 3 cut(s) 411, 465, 738
BseLI CCNNNNNNNGG 5 cut(s) 409, 712, 741, 827, 968
BseNI ACTGG 1 cut(s) 978
BsePI GCGCGC 1 cut(s) 597
BseRI GAGGAG 5 cut(s) 284, 374, 379, 404, 425
BseX3I CGGCCG 1 cut(s) 571
BseXI GCAGC 3 cut(s) 764, 906, 1018
Bsh1236I CGCG 3 cut(s) 98, 167, 599
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 4 cut(s) 5, 573, 619, 662
BshNI GGYRCC 1 cut(s) 744
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 1 cut(s) 480
BsiHKCI CYCGRG 2 cut(s) 219, 727
BsiSI CCGG 3 cut(s) 83, 403, 742
BslFI GGGAC 6 cut(s) 146, 278, 308, 314, 555, 913
BslI CCNNNNNNNGG 5 cut(s) 409, 712, 741, 827, 968
BsmAI GTCTC 4 cut(s) 188, 434, 924, 936
BsmBI CGTCTC 1 cut(s) 434
BsmFI GGGAC 6 cut(s) 146, 278, 308, 314, 555, 913
BsnI GGCC 4 cut(s) 5, 573, 619, 662
BsoBI CYCGRG 2 cut(s) 219, 727
Bsp119I TTCGAA 2 cut(s) 236, 420
Bsp1286I GDGCHC 1 cut(s) 480
Bsp143I GATC 4 cut(s) 67, 304, 354, 606
BspANI GGCC 4 cut(s) 5, 573, 619, 662
BspFNI CGCG 3 cut(s) 98, 167, 599
BspLI GGNNCC 5 cut(s) 134, 135, 203, 312, 746
BspT104I TTCGAA 2 cut(s) 236, 420
BspT107I GGYRCC 1 cut(s) 744
BsrBI CCGCTC 1 cut(s) 508
BsrI ACTGG 1 cut(s) 978
BssECI CCNNGG 4 cut(s) 6, 50, 516, 614
BssHII GCGCGC 1 cut(s) 597
BssMI GATC 4 cut(s) 67, 304, 354, 606
BssNI GRCGYC 2 cut(s) 39, 745
BssT1I CCWWGG 1 cut(s) 614
Bst4CI ACNGT 1 cut(s) 904
Bst6I CTCTTC 3 cut(s) 335, 432, 798
BstACI GRCGYC 2 cut(s) 39, 745
BstBI TTCGAA 2 cut(s) 236, 420
BstC8I GCNNGC 6 cut(s) 206, 501, 599, 654, 736, 777
BstDEI CTNAG 1 cut(s) 492
BstDSI CCRYGG 2 cut(s) 6, 516
BstEII GGTNACC 1 cut(s) 811
BstF5I GGATG 3 cut(s) 411, 465, 738
BstFNI CGCG 3 cut(s) 98, 167, 599
BstH2I RGCGCY 2 cut(s) 92, 748
BstHHI GCGC 7 cut(s) 91, 100, 523, 599, 601, 747, 1036
BstKTI GATC 4 cut(s) 70, 307, 357, 609
BstMAI GTCTC 4 cut(s) 188, 434, 924, 936
BstMBI GATC 4 cut(s) 67, 304, 354, 606
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 8 cut(s) 95, 97, 136, 454, 505, 570, 668, 744
BstNSI RCATGY 1 cut(s) 568
BstPI GGTNACC 1 cut(s) 811
BstUI CGCG 3 cut(s) 98, 167, 599
BstV1I GCAGC 3 cut(s) 764, 906, 1018
BstV2I GAAGAC 2 cut(s) 80, 429
BstZI CGGCCG 1 cut(s) 571
BsuRI GGCC 4 cut(s) 5, 573, 619, 662
BtgI CCRYGG 2 cut(s) 6, 516
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 3 cut(s) 411, 465, 738
BtsIMutI CAGTG 1 cut(s) 909
Cac8I GCNNGC 6 cut(s) 206, 501, 599, 654, 736, 777
CfoI GCGC 7 cut(s) 91, 100, 523, 599, 601, 747, 1036
Cfr13I GGNCC 3 cut(s) 54, 133, 618
CseI GACGC 1 cut(s) 104
Csp6I GTAC 3 cut(s) 289, 568, 766
CviAII CATG 2 cut(s) 109, 565
CviQI GTAC 3 cut(s) 289, 568, 766
DdeI CTNAG 1 cut(s) 492
DinI GGCGCC 1 cut(s) 746
DpnI GATC 4 cut(s) 69, 306, 356, 608
DpnII GATC 4 cut(s) 67, 304, 354, 606
DriI GACNNNNNGTC 1 cut(s) 43
EaeI YGGCCR 2 cut(s) 3, 571
EagI CGGCCG 1 cut(s) 571
Eam1104I CTCTTC 3 cut(s) 335, 432, 798
Eam1105I GACNNNNNGTC 1 cut(s) 43
EarI CTCTTC 3 cut(s) 335, 432, 798
EciI GGCGGA 2 cut(s) 401, 518
Ecl136II GAGCTC 1 cut(s) 478
EclXI CGGCCG 1 cut(s) 571
Eco130I CCWWGG 1 cut(s) 614
Eco24I GRGCYC 1 cut(s) 480
Eco47I GGWCC 2 cut(s) 54, 133
Eco52I CGGCCG 1 cut(s) 571
Eco53kI GAGCTC 1 cut(s) 478
Eco88I CYCGRG 2 cut(s) 219, 727
Eco91I GGTNACC 1 cut(s) 811
EcoICRI GAGCTC 1 cut(s) 478
EcoO109I RGGNCCY 1 cut(s) 133
EcoO65I GGTNACC 1 cut(s) 811
EcoRI GAATTC 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 614
EcoT38I GRGCYC 1 cut(s) 480
EgeI GGCGCC 1 cut(s) 746
EheI GGCGCC 1 cut(s) 746
ErhI CCWWGG 1 cut(s) 614
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 2 cut(s) 112, 568
FaqI GGGAC 6 cut(s) 146, 278, 308, 314, 555, 913
FatI CATG 2 cut(s) 108, 564
FauI CCCGC 3 cut(s) 123, 144, 890
Fnu4HI GCNGC 3 cut(s) 753, 895, 1032
FokI GGATG 3 cut(s) 418, 472, 725
FriOI GRGCYC 1 cut(s) 480
Fsp4HI GCNGC 3 cut(s) 753, 895, 1032
FspBI CTAG 6 cut(s) 23, 35, 615, 834, 891, 927
GlaI GCGC 7 cut(s) 90, 99, 522, 598, 600, 746, 1035
GluI GCNGC 3 cut(s) 753, 895, 1032
GsuI CTGGAG 1 cut(s) 995
HaeII RGCGCY 2 cut(s) 92, 748
HaeIII GGCC 4 cut(s) 5, 573, 619, 662
HapII CCGG 3 cut(s) 83, 403, 742
HgaI GACGC 1 cut(s) 104
HhaI GCGC 7 cut(s) 91, 100, 523, 599, 601, 747, 1036
Hin1I GRCGYC 2 cut(s) 39, 745
Hin1II CATG 2 cut(s) 112, 568
Hin6I GCGC 7 cut(s) 89, 98, 521, 597, 599, 745, 1034
HinP1I GCGC 7 cut(s) 89, 98, 521, 597, 599, 745, 1034
HinfI GANTC 5 cut(s) 76, 142, 182, 196, 210
HpaII CCGG 3 cut(s) 83, 403, 742
HphI GGTGA 2 cut(s) 358, 647
Hpy166II GTNNAC 2 cut(s) 244, 253
Hpy188I TCNGA 6 cut(s) 17, 72, 187, 201, 941, 971
Hpy188III TCNNGA 6 cut(s) 65, 308, 604, 790, 927, 994
Hpy8I GTNNAC 2 cut(s) 244, 253
Hpy99I CGWCG 4 cut(s) 49, 98, 431, 925
HpyAV CCTTC 7 cut(s) 232, 322, 343, 376, 541, 552, 587
HpyCH4III ACNGT 1 cut(s) 904
HpyCH4IV ACGT 4 cut(s) 39, 44, 623, 857
HpyCH4V TGCA 2 cut(s) 693, 775
HpyF10VI GCNNNNNNNGC 8 cut(s) 95, 97, 136, 454, 505, 570, 668, 744
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 4 cut(s) 39, 44, 623, 857
Hsp92I GRCGYC 2 cut(s) 39, 745
Hsp92II CATG 2 cut(s) 112, 568
HspAI GCGC 7 cut(s) 89, 98, 521, 597, 599, 745, 1034
KasI GGCGCC 1 cut(s) 744
KflI GGGWCCC 1 cut(s) 133
Kzo9I GATC 4 cut(s) 67, 304, 354, 606
LmnI GCTCC 6 cut(s) 201, 310, 445, 496, 505, 1028
Lsp1109I GCAGC 3 cut(s) 764, 906, 1018
LweI GCATC 1 cut(s) 747
MaeI CTAG 6 cut(s) 23, 35, 615, 834, 891, 927
MaeII ACGT 4 cut(s) 39, 44, 623, 857
MaeIII GTNAC 4 cut(s) 40, 143, 811, 904
MalI GATC 4 cut(s) 69, 306, 356, 608
MbiI CCGCTC 1 cut(s) 508
MboI GATC 4 cut(s) 67, 304, 354, 606
MboII GAAGA 7 cut(s) 85, 352, 391, 434, 449, 730, 815
MfeI CAATTG 1 cut(s) 710
MhlI GDGCHC 1 cut(s) 480
MluCI AATT 4 cut(s) 60, 232, 645, 710
Mly113I GGCGCC 1 cut(s) 745
MlyI GAGTC 5 cut(s) 70, 151, 176, 190, 219
MmeI TCCRAC 3 cut(s) 297, 354, 1006
MslI CAYNNNNRTG 1 cut(s) 729
MspA1I CMGCKG 1 cut(s) 897
MspI CCGG 3 cut(s) 83, 403, 742
MunI CAATTG 1 cut(s) 710
MvnI CGCG 3 cut(s) 98, 167, 599
MwoI GCNNNNNNNGC 8 cut(s) 95, 97, 136, 454, 505, 570, 668, 744
NarI GGCGCC 1 cut(s) 745
NdeII GATC 4 cut(s) 67, 304, 354, 606
NlaIII CATG 2 cut(s) 112, 568
NlaIV GGNNCC 5 cut(s) 134, 135, 203, 312, 746
NmeAIII GCCGAG 1 cut(s) 75
NmuCI GTSAC 3 cut(s) 40, 143, 904
NspI RCATGY 1 cut(s) 568
NspV TTCGAA 2 cut(s) 236, 420
OliI CACNNNNGTG 1 cut(s) 729
PauI GCGCGC 1 cut(s) 597
PcsI WCGNNNNNNNCGW 3 cut(s) 76, 205, 264
PflMI CCANNNNNTGG 2 cut(s) 712, 827
PkrI GCNGC 3 cut(s) 754, 896, 1033
PleI GAGTC 5 cut(s) 70, 150, 176, 190, 218
PluTI GGCGCC 1 cut(s) 748
PpsI GAGTC 5 cut(s) 70, 150, 176, 190, 218
PpuMI RGGWCCY 1 cut(s) 133
Psp124BI GAGCTC 1 cut(s) 480
Psp5II RGGWCCY 1 cut(s) 133
PspEI GGTNACC 1 cut(s) 811
PspN4I GGNNCC 5 cut(s) 134, 135, 203, 312, 746
PspPI GGNCC 3 cut(s) 54, 133, 618
PspPPI RGGWCCY 1 cut(s) 133
PteI GCGCGC 1 cut(s) 597
RsaI GTAC 3 cut(s) 290, 569, 767
RsaNI GTAC 3 cut(s) 289, 568, 766
RseI CAYNNNNRTG 1 cut(s) 729
SacI GAGCTC 1 cut(s) 480
SatI GCNGC 3 cut(s) 753, 895, 1032
Sau3AI GATC 4 cut(s) 67, 304, 354, 606
Sau96I GGNCC 3 cut(s) 54, 133, 618
SchI GAGTC 5 cut(s) 70, 151, 176, 190, 219
SduI GDGCHC 1 cut(s) 480
SfaNI GCATC 1 cut(s) 747
SfoI GGCGCC 1 cut(s) 746
SfuI TTCGAA 2 cut(s) 236, 420
SinI GGWCC 2 cut(s) 54, 133
SmiMI CAYNNNNRTG 1 cut(s) 729
SmlI CTYRAG 1 cut(s) 788
SmoI CTYRAG 1 cut(s) 788
Sse9I AATT 4 cut(s) 60, 232, 645, 710
SspDI GGCGCC 1 cut(s) 744
SspMI CTAG 6 cut(s) 23, 35, 615, 834, 891, 927
SstI GAGCTC 1 cut(s) 480
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 1 cut(s) 904
TaiI ACGT 4 cut(s) 42, 47, 626, 860
TaqI TCGA 6 cut(s) 93, 236, 258, 357, 420, 480
TasI AATT 4 cut(s) 60, 232, 645, 710
TscAI CASTG 1 cut(s) 909
TseFI GTSAC 3 cut(s) 40, 143, 904
TseI GCWGC 3 cut(s) 752, 894, 1031
Tsp45I GTSAC 3 cut(s) 40, 143, 904
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 1 cut(s) 458
TspRI CASTG 1 cut(s) 909
Van91I CCANNNNNTGG 2 cut(s) 712, 827
VpaK11BI GGWCC 2 cut(s) 54, 133
XapI RAATTY 2 cut(s) 232, 645
XbaI TCTAGA 1 cut(s) 926
XceI RCATGY 1 cut(s) 568
XmaJI CCTAGG 1 cut(s) 614
XspI CTAG 6 cut(s) 23, 35, 615, 834, 891, 927
ZraI GACGTC 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.