FvH4_4g25740

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014723
Physical Location & Seq
Forward (+)
27431099 .. 27432767
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25740.t1

Sequence Viewer

Length: 960 bp
ATGCATGATGCAATTGGAATTCCTGCTTGCTTTTCGGCCGGTGCTGCTCATCAGAGGCCGGCTGATCATGATCCGGCGGGTGTGATCAGGTCAGGACAGAGCTTGGTTATGTCAGTTTATAGTACAAAGATTGCTGGCCGGTGCCGGTTGATCACAGTTACTTGGTGCAAGAACCTACTGCTACATGGCCTCTCTGTTTCTGTTCAAGGCCAAGAAGGAGAGGATGCCTATAGATGCAAGGTTGAGCTGAAGCCCTGGTACTTTTGGAGGAAGACTGGCGCCAAACAGTTCATGGTGGATGGTATCACAGTTGATGTAGTCTGGGACCTCAAAGCTGCCAAGTTCAATGGTGAAACCGAGCCACAGTCGGACTATTATGTTGCCTTTGTGTGTGAAGAAGAGGTGGTTCTGCTTGTTGGTGATCAGAAGAAAGATGCCTATAGAAGAACAGGTTGCAGACCATCACTGATTGAGCCTATCTTGGTTTCAAGGAAGGAGCATTTGTTTGGTAAGAGGAAGTACTCAACAGGGATCCAGTTTCATGAACAAGGAAAGGGAAAGTTTCATGAGATTTTAATCGAGTGCAACAACAACAGCAACAATTCTGTAACCGGGTCCGGGGCTGATCCAGAGTTGGAGATAAAGATTGATGGGAACCAAGCCATTCATGTCAAGCACCTTCAATGGAAGTTCAGAGGCAATGAGTCTGTTGATGTGAACAAAACCAAAGTTGAAGTCTATTATGATGTTCATGATTGGCTCTTTGGCTCTGGTCCAAGGTATGGGATGTTCATATTCAAGCCAATACCATCAGAATCTGCAGAGTCTTCTTTATCAACAACAGCCCAAGTTTTGACTCATCATCAAAAATCAAGGGGTGGATTAGAAGATGAGGATGATGATAGTGCAGGTGGGTCATCTGGATTTTCCTTACTGGTTTATGCTTGGAAAGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

320

Amino Acids

35.59

Weight (kDa)

6.32

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 31 - 317 8.5e-103 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014723)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25740
malus_domestica MD13G1099400.v1.1 MD16G1100500.v1.1
prunus_persica Prupe.1G246500_v2.0.a1
pyrus_communis pycom13g08680 pycom16g08520
rosa_chinensis RchiOBHm_Chr4g0433181
rosa_laevigata RLG00000006776
rosa_multiflora Rmu_ssc0000295.1_g000012
rosa_roxburghii Rroxscaffold_5G00374680
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320300 Rh4BG328500 Rh4CG343900 Rh4DG323900
rosa_wichuraiana Rw0G008710 Rw4G027810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 899
Acc36I ACCTGC 1 cut(s) 899
AccB1I GGYRCC 2 cut(s) 141, 278
AccB7I CCANNNNNTGG 1 cut(s) 782
AciI CCGC 1 cut(s) 77
AclWI GGATC 4 cut(s) 65, 526, 539, 620
AcoI YGGCCR 2 cut(s) 36, 136
AcsI RAATTY 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 3 cut(s) 124, 260, 521
AfiI CCNNNNNNNGG 2 cut(s) 618, 782
AgsI TTSAA 6 cut(s) 206, 346, 489, 683, 734, 799
AjnI CCWGG 1 cut(s) 254
AluBI AGCT 3 cut(s) 102, 247, 335
AluI AGCT 3 cut(s) 102, 247, 335
AlwI GGATC 4 cut(s) 65, 526, 539, 620
AlwNI CAGNNNCTG 1 cut(s) 818
AoxI GGCC 5 cut(s) 36, 56, 136, 187, 208
ApeKI GCWGC 2 cut(s) 44, 335
ApoI RAATTY 1 cut(s) 18
ArsI GACNNNNNNTTYG 2 cut(s) 720, 752
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 325, 615, 773
AsuC2I CCSGG 2 cut(s) 613, 619
AsuHPI GGTGA 2 cut(s) 362, 431
AvaII GGWCC 3 cut(s) 325, 615, 773
BamHI GGATCC 1 cut(s) 531
BanI GGYRCC 2 cut(s) 141, 278
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BbsI GAAGAC 2 cut(s) 278, 819
BbvI GCAGC 2 cut(s) 31, 322
BccI CCATC 4 cut(s) 293, 469, 644, 817
BciT130I CCWGG 1 cut(s) 256
BclI TGATCA 4 cut(s) 64, 84, 150, 421
BcnI CCSGG 2 cut(s) 613, 619
BfmI CTRYAG 3 cut(s) 229, 439, 819
BfoI RGCGCY 1 cut(s) 282
BfuAI ACCTGC 1 cut(s) 899
BisI GCNGC 2 cut(s) 45, 336
BlsI GCNGC 2 cut(s) 46, 337
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 3 cut(s) 256, 613, 619
Bme18I GGWCC 3 cut(s) 325, 615, 773
BmgT120I GGNCC 3 cut(s) 325, 615, 773
BmiI GGNNCC 6 cut(s) 143, 280, 326, 533, 616, 656
BmrFI CCNGG 3 cut(s) 256, 613, 619
BmsI GCATC 3 cut(s) 214, 224, 424
BpiI GAAGAC 2 cut(s) 278, 819
BpuMI CCSGG 2 cut(s) 613, 619
BsaBI GATNNNNATC 2 cut(s) 69, 575
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 254, 618, 776
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 2 cut(s) 618, 782
Bse118I RCCGGY 4 cut(s) 38, 58, 138, 144
Bse1I ACTGG 3 cut(s) 280, 535, 939
Bse3DI GCAATG 1 cut(s) 706
Bse8I GATNNNNATC 2 cut(s) 69, 575
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 3 cut(s) 254, 618, 776
BseGI GGATG 4 cut(s) 229, 304, 792, 901
BseJI GATNNNNATC 2 cut(s) 69, 575
BseLI CCNNNNNNNGG 2 cut(s) 618, 782
BseMI GCAATG 1 cut(s) 706
BseNI ACTGG 3 cut(s) 280, 535, 939
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 2 cut(s) 31, 322
BsgI GTGCAG 1 cut(s) 927
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 5 cut(s) 38, 58, 138, 189, 210
BshNI GGYRCC 2 cut(s) 141, 278
BsiEI CGRYCG 1 cut(s) 39
BsiSI CCGG 7 cut(s) 39, 59, 74, 139, 145, 612, 618
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 2 cut(s) 618, 782
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 5 cut(s) 38, 58, 138, 189, 210
Bsp143I GATC 7 cut(s) 64, 70, 84, 150, 421, 531, 625
BspACI CCGC 1 cut(s) 77
BspANI GGCC 5 cut(s) 38, 58, 138, 189, 210
BspHI TCATGA 4 cut(s) 67, 541, 565, 751
BspLI GGNNCC 6 cut(s) 143, 280, 326, 533, 616, 656
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 899
BspPI GGATC 4 cut(s) 65, 526, 539, 620
BspT107I GGYRCC 2 cut(s) 141, 278
BsrDI GCAATG 1 cut(s) 706
BsrFI RCCGGY 4 cut(s) 38, 58, 138, 144
BsrI ACTGG 3 cut(s) 280, 535, 939
BssAI RCCGGY 4 cut(s) 38, 58, 138, 144
BssECI CCNNGG 3 cut(s) 254, 618, 776
BssMI GATC 7 cut(s) 64, 70, 84, 150, 421, 531, 625
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 776
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 4 cut(s) 157, 288, 310, 366
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 279
BstC8I GCNNGC 3 cut(s) 28, 60, 136
BstF5I GGATG 4 cut(s) 229, 304, 792, 901
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 7 cut(s) 67, 73, 87, 153, 424, 534, 628
BstMBI GATC 7 cut(s) 64, 70, 84, 150, 421, 531, 625
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 1 cut(s) 44
BstNI CCWGG 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 254, 611, 617
BstSFI CTRYAG 3 cut(s) 229, 439, 819
BstV1I GCAGC 2 cut(s) 31, 322
BstV2I GAAGAC 2 cut(s) 278, 819
BstX2I RGATCY 1 cut(s) 531
BstYI RGATCY 1 cut(s) 531
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 5 cut(s) 38, 58, 138, 189, 210
BtsCI GGATG 4 cut(s) 229, 304, 792, 901
BtsIMutI CAGTG 1 cut(s) 464
BveI ACCTGC 1 cut(s) 899
Cac8I GCNNGC 3 cut(s) 28, 60, 136
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 4 cut(s) 67, 541, 565, 751
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 4 cut(s) 38, 58, 138, 144
Cfr13I GGNCC 3 cut(s) 325, 615, 773
Csp6I GTAC 3 cut(s) 123, 259, 520
CviAII CATG 8 cut(s) 5, 68, 185, 292, 542, 566, 668, 752
CviQI GTAC 3 cut(s) 123, 259, 520
DinI GGCGCC 1 cut(s) 280
DpnI GATC 7 cut(s) 66, 72, 86, 152, 423, 533, 627
DpnII GATC 7 cut(s) 64, 70, 84, 150, 421, 531, 625
EaeI YGGCCR 2 cut(s) 36, 136
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 776
Eco47I GGWCC 3 cut(s) 325, 615, 773
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 776
EcoT22I ATGCAT 1 cut(s) 6
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 776
FaeI CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 671, 755
FaqI GGGAC 1 cut(s) 338
FatI CATG 8 cut(s) 4, 67, 184, 291, 541, 565, 667, 751
FauI CCCGC 1 cut(s) 70
FbaI TGATCA 4 cut(s) 64, 84, 150, 421
Fnu4HI GCNGC 2 cut(s) 45, 336
FokI GGATG 4 cut(s) 236, 311, 799, 908
Fsp4HI GCNGC 2 cut(s) 45, 336
GlaI GCGC 1 cut(s) 280
GluI GCNGC 2 cut(s) 45, 336
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 5 cut(s) 38, 58, 138, 189, 210
HapII CCGG 7 cut(s) 39, 59, 74, 139, 145, 612, 618
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 671, 755
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 4 cut(s) 704, 815, 824, 856
HpaII CCGG 7 cut(s) 39, 59, 74, 139, 145, 612, 618
HphI GGTGA 2 cut(s) 362, 431
Hpy166II GTNNAC 1 cut(s) 718
Hpy188I TCNGA 5 cut(s) 54, 370, 426, 695, 814
Hpy188III TCNNGA 7 cut(s) 68, 93, 542, 566, 629, 752, 921
Hpy8I GTNNAC 1 cut(s) 718
HpyAV CCTTC 3 cut(s) 209, 487, 689
HpyCH4III ACNGT 4 cut(s) 157, 288, 310, 366
HpyCH4V TGCA 8 cut(s) 4, 11, 168, 237, 456, 585, 821, 908
HpyF10VI GCNNNNNNNGC 1 cut(s) 44
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 671, 755
HspAI GCGC 1 cut(s) 279
KasI GGCGCC 1 cut(s) 278
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
Ksp22I TGATCA 4 cut(s) 64, 84, 150, 421
Kzo9I GATC 7 cut(s) 64, 70, 84, 150, 421, 531, 625
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 2 cut(s) 31, 322
LweI GCATC 3 cut(s) 214, 224, 424
MaeIII GTNAC 2 cut(s) 157, 607
MalI GATC 7 cut(s) 66, 72, 86, 152, 423, 533, 627
MboI GATC 7 cut(s) 64, 70, 84, 150, 421, 531, 625
MboII GAAGA 7 cut(s) 283, 407, 410, 439, 456, 819, 899
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 1 cut(s) 531
MluCI AATT 3 cut(s) 12, 18, 601
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 3 cut(s) 713, 833, 850
MmeI TCCRAC 2 cut(s) 348, 615
MnlI CCTC 9 cut(s) 48, 200, 214, 261, 338, 394, 507, 689, 886
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 58
MseI TTAA 1 cut(s) 575
MspI CCGG 7 cut(s) 39, 59, 74, 139, 145, 612, 618
MspR9I CCNGG 3 cut(s) 256, 613, 619
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 256
MwoI GCNNNNNNNGC 1 cut(s) 44
NaeI GCCGGC 1 cut(s) 60
NarI GGCGCC 1 cut(s) 279
NciI CCSGG 2 cut(s) 613, 619
NdeII GATC 7 cut(s) 64, 70, 84, 150, 421, 531, 625
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 671, 755
NlaIV GGNNCC 6 cut(s) 143, 280, 326, 533, 616, 656
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 4 cut(s) 67, 541, 565, 751
PaqCI CACCTGC 1 cut(s) 899
PdiI GCCGGC 1 cut(s) 60
PfeI GAWTC 1 cut(s) 815
PflMI CCANNNNNTGG 1 cut(s) 782
PkrI GCNGC 2 cut(s) 46, 337
PleI GAGTC 3 cut(s) 712, 832, 850
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 3 cut(s) 712, 832, 850
PpuMI RGGWCCY 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 6 cut(s) 143, 280, 326, 533, 616, 656
PspPI GGNCC 3 cut(s) 325, 615, 773
PspPPI RGGWCCY 1 cut(s) 325
PsrI GAACNNNNNNTAC 2 cut(s) 773, 805
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 531
RsaI GTAC 3 cut(s) 124, 260, 521
RsaNI GTAC 3 cut(s) 123, 259, 520
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 2 cut(s) 45, 336
Sau3AI GATC 7 cut(s) 64, 70, 84, 150, 421, 531, 625
Sau96I GGNCC 3 cut(s) 325, 615, 773
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 3 cut(s) 713, 833, 850
ScrFI CCNGG 3 cut(s) 256, 613, 619
SfaNI GCATC 3 cut(s) 214, 224, 424
SfcI CTRYAG 3 cut(s) 229, 439, 819
SfoI GGCGCC 1 cut(s) 280
SinI GGWCC 3 cut(s) 325, 615, 773
Sse9I AATT 3 cut(s) 12, 18, 601
SsiI CCGC 1 cut(s) 77
SspDI GGCGCC 1 cut(s) 278
StyD4I CCNGG 3 cut(s) 254, 611, 617
StyI CCWWGG 1 cut(s) 776
TaaI ACNGT 4 cut(s) 157, 288, 310, 366
TaqI TCGA 1 cut(s) 579
TasI AATT 3 cut(s) 12, 18, 601
TatI WGTACW 2 cut(s) 122, 519
TfiI GAWTC 1 cut(s) 815
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 1 cut(s) 471
TseI GCWGC 2 cut(s) 44, 335
TspDTI ATGAA 7 cut(s) 280, 530, 554, 558, 656, 740, 781
TspRI CASTG 1 cut(s) 471
Van91I CCANNNNNTGG 1 cut(s) 782
VpaK11BI GGWCC 3 cut(s) 325, 615, 773
XapI RAATTY 1 cut(s) 18
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
ZrmI AGTACT 1 cut(s) 521
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.