MD16G1100500.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014723
Physical Location & Seq
Reverse (-)
6963494 .. 6964426
933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1100500.v1.1.491

Sequence Viewer

Length: 933 bp
ATGCACGATTCAATTGGAATTCCGGCCTGCTTCACAGCCGGCACTCCAAGGCCGGCCGACGATCCAACCCCAACCGGCGTGATCAGGTCGGGGCAGAGCGTGTTCATGTCAGTGTACAGCACAAAGATAGCCGGCCGGTGCCGGCTGATCACCGTCACCTGGTGCAAGAATCTGTTACTCCACGGCCTCTCTGTTTCGGTGCAAGGCCAGAACAGAGACGAAGAGTACCGGTGCAAAGTTGAGCTCAAGCCGTGGTACTTCTGGCGCAAGCAAGGCTCCAAGCAATTCACGGTGGACGGCATCACCGTGGACGTCGTCTGGGACCTCAAGGCCGCCAAATTCAACGGCGAAACGGAGCCACAATCGGACTACTACGTCGCCATTGTCTGCGAAGAGGAGGTGGTTTTGCTCGTTGGCGATCAGAAAAAAGACGCATTCAGAAAAACTGGCTGCCGGCCGTCGCTCATAGAGCCGATTCTGGTTTCGAGGAGGGAGCATTTGTTCGGGAAGAGGAAGTTTTCAACACGAATCAAGTTTCACGAGAAGGAGAATTTCCATGAGATTTTGATCGAGTGCAGCAACGGCTCAGATGGGTCTGATCCGGAGTTGGAGATAAAGATTGATGGGATCCAAGCCGTTCGTGTGAAGCGGCTTCACTGGAAGTTCAGAGGCAATGATTGTGTTGATGTGAACAGAAACAGAGTTGAAGTTTTCTGGGATGTTCATGATTGGCTGTTTTGCTCCGGTCCGAGGCACGGAATGTTTATATTTAAGCCGGTGTCGGCGGAGCATACGGCTGAGCCCCCGTCGCTTTCGGCCGTTGACCAATCTTTGACCGAAAAGGTAGGTTCTGGTGTGGTAGTCGAGCATGATAACGCGGGTGGGGCATCCTCTGTGTTTTCTTTATTTGTCTATGCGTGGAGGGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

311

Amino Acids

35.05

Weight (kDa)

6.8

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 30 - 308 4.8e-105 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014723)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25740
malus_domestica MD13G1099400.v1.1 MD16G1100500.v1.1
prunus_persica Prupe.1G246500_v2.0.a1
pyrus_communis pycom13g08680 pycom16g08520
rosa_chinensis RchiOBHm_Chr4g0433181
rosa_laevigata RLG00000006776
rosa_multiflora Rmu_ssc0000295.1_g000012
rosa_roxburghii Rroxscaffold_5G00374680
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320300 Rh4BG328500 Rh4CG343900 Rh4DG323900
rosa_wichuraiana Rw0G008710 Rw4G027810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 374
AatII GACGTC 1 cut(s) 315
AccB1I GGYRCC 1 cut(s) 138
AccII CGCG 1 cut(s) 878
AccIII TCCGGA 1 cut(s) 601
AciI CCGC 4 cut(s) 333, 649, 785, 878
AclWI GGATC 4 cut(s) 56, 593, 622, 635
AcoI YGGCCR 4 cut(s) 54, 133, 455, 816
AcsI RAATTY 3 cut(s) 18, 338, 550
AcyI GRCGYC 1 cut(s) 312
AdeI CACNNNGTG 1 cut(s) 162
AfaI GTAC 3 cut(s) 116, 227, 257
AfiI CCNNNNNNNGG 3 cut(s) 159, 750, 755
AgeI ACCGGT 1 cut(s) 228
AgsI TTSAA 4 cut(s) 12, 343, 522, 707
AjnI CCWGG 1 cut(s) 158
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
AluBI AGCT 1 cut(s) 244
AluI AGCT 1 cut(s) 244
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 210
AlwI GGATC 4 cut(s) 56, 593, 622, 635
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 9 cut(s) 24, 50, 54, 133, 184, 205, 330, 455, 816
ApeKI GCWGC 2 cut(s) 450, 576
ApoI RAATTY 3 cut(s) 18, 338, 550
AsiGI ACCGGT 1 cut(s) 228
AspLEI GCGC 1 cut(s) 267
AspS9I GGNCC 2 cut(s) 322, 746
AsuHPI GGTGA 3 cut(s) 142, 148, 295
AvaII GGWCC 2 cut(s) 322, 746
BamHI GGATCC 1 cut(s) 627
BanI GGYRCC 1 cut(s) 138
BanII GRGCYC 2 cut(s) 246, 804
BauI CACGAG 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 2 cut(s) 437, 588
BccI CCATC 2 cut(s) 584, 617
BceAI ACGGC 9 cut(s) 199, 235, 313, 361, 442, 598, 620, 803, 810
BciT130I CCWGG 1 cut(s) 160
BclI TGATCA 2 cut(s) 81, 147
BcoDI GTCTC 1 cut(s) 210
BisI GCNGC 4 cut(s) 333, 451, 577, 650
BlpI GCTNAGC 1 cut(s) 798
BlsI GCNGC 4 cut(s) 334, 452, 578, 651
Bme1390I CCNGG 1 cut(s) 160
Bme18I GGWCC 2 cut(s) 322, 746
BmgT120I GGNCC 2 cut(s) 322, 746
BmiI GGNNCC 5 cut(s) 140, 277, 323, 357, 629
BmrFI CCNGG 1 cut(s) 160
BmsI GCATC 2 cut(s) 309, 896
Bpu1102I GCTNAGC 1 cut(s) 798
BpuEI CTTGAG 2 cut(s) 230, 311
BsaBI GATNNNNATC 1 cut(s) 566
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 5 cut(s) 47, 181, 251, 306, 749
BsaWI WCCGGW 3 cut(s) 228, 601, 743
BsaXI ACNNNNNCTCC 2 cut(s) 485, 515
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 750, 755
Bse118I RCCGGY 9 cut(s) 38, 52, 74, 131, 135, 141, 228, 453, 775
Bse1I ACTGG 2 cut(s) 451, 662
Bse3DI GCAATG 1 cut(s) 679
Bse8I GATNNNNATC 1 cut(s) 566
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 1 cut(s) 160
BseDI CCNNGG 5 cut(s) 47, 181, 251, 306, 749
BseGI GGATG 2 cut(s) 724, 887
BseJI GATNNNNATC 1 cut(s) 566
BseLI CCNNNNNNNGG 3 cut(s) 159, 750, 755
BseMI GCAATG 1 cut(s) 679
BseMII CTCAG 2 cut(s) 600, 789
BseNI ACTGG 2 cut(s) 451, 662
BseRI GAGGAG 2 cut(s) 410, 502
BseX3I CGGCCG 4 cut(s) 54, 133, 455, 816
BseXI GCAGC 2 cut(s) 437, 588
BsgI GTGCAG 1 cut(s) 595
Bsh1236I CGCG 1 cut(s) 878
Bsh1285I CGRYCG 4 cut(s) 57, 136, 458, 819
BshFI GGCC 9 cut(s) 26, 52, 56, 135, 186, 207, 332, 457, 818
BshNI GGYRCC 1 cut(s) 138
BshTI ACCGGT 1 cut(s) 228
BsiEI CGRYCG 4 cut(s) 57, 136, 458, 819
BsiHKAI GWGCWC 1 cut(s) 246
BslFI GGGAC 1 cut(s) 335
BslI CCNNNNNNNGG 3 cut(s) 159, 750, 755
BsmAI GTCTC 1 cut(s) 210
BsmBI CGTCTC 1 cut(s) 210
BsmFI GGGAC 1 cut(s) 335
BsmI GAATGC 1 cut(s) 434
BsnI GGCC 9 cut(s) 26, 52, 56, 135, 186, 207, 332, 457, 818
Bsp1286I GDGCHC 2 cut(s) 246, 804
Bsp13I TCCGGA 1 cut(s) 601
Bsp1407I TGTACA 1 cut(s) 114
Bsp143I GATC 7 cut(s) 61, 81, 147, 418, 567, 598, 627
Bsp1720I GCTNAGC 1 cut(s) 798
BspACI CCGC 4 cut(s) 333, 649, 785, 878
BspANI GGCC 9 cut(s) 26, 52, 56, 135, 186, 207, 332, 457, 818
BspCNI CTCAG 2 cut(s) 599, 790
BspEI TCCGGA 1 cut(s) 601
BspFNI CGCG 1 cut(s) 878
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 5 cut(s) 140, 277, 323, 357, 629
BspPI GGATC 4 cut(s) 56, 593, 622, 635
BspT107I GGYRCC 1 cut(s) 138
BsrDI GCAATG 1 cut(s) 679
BsrFI RCCGGY 9 cut(s) 38, 52, 74, 131, 135, 141, 228, 453, 775
BsrGI TGTACA 1 cut(s) 114
BsrI ACTGG 2 cut(s) 451, 662
BssAI RCCGGY 9 cut(s) 38, 52, 74, 131, 135, 141, 228, 453, 775
BssECI CCNNGG 5 cut(s) 47, 181, 251, 306, 749
BssMI GATC 7 cut(s) 61, 81, 147, 418, 567, 598, 627
BssNI GRCGYC 1 cut(s) 312
BssSI CACGAG 1 cut(s) 539
BssT1I CCWWGG 1 cut(s) 47
Bst2BI CACGAG 1 cut(s) 539
Bst2UI CCWGG 1 cut(s) 160
Bst4CI ACNGT 3 cut(s) 154, 292, 307
Bst6I CTCTTC 3 cut(s) 216, 387, 503
BstACI GRCGYC 1 cut(s) 312
BstAUI TGTACA 1 cut(s) 114
BstC8I GCNNGC 7 cut(s) 28, 40, 54, 133, 143, 269, 455
BstDEI CTNAG 2 cut(s) 586, 798
BstDSI CCRYGG 3 cut(s) 181, 251, 306
BstF5I GGATG 2 cut(s) 724, 887
BstFNI CGCG 1 cut(s) 878
BstHHI GCGC 1 cut(s) 267
BstKTI GATC 7 cut(s) 64, 84, 150, 421, 570, 601, 630
BstMAI GTCTC 1 cut(s) 210
BstMBI GATC 7 cut(s) 61, 81, 147, 418, 567, 598, 627
BstMCI CGRYCG 4 cut(s) 57, 136, 458, 819
BstMWI GCNNNNNNNGC 5 cut(s) 273, 469, 582, 808, 884
BstNI CCWGG 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 158
BstUI CGCG 1 cut(s) 878
BstV1I GCAGC 2 cut(s) 437, 588
BstX2I RGATCY 1 cut(s) 627
BstYI RGATCY 1 cut(s) 627
BstZI CGGCCG 4 cut(s) 54, 133, 455, 816
BsuRI GGCC 9 cut(s) 26, 52, 56, 135, 186, 207, 332, 457, 818
BtgI CCRYGG 3 cut(s) 181, 251, 306
BtsCI GGATG 2 cut(s) 724, 887
BtsIMutI CAGTG 2 cut(s) 117, 655
Cac8I GCNNGC 7 cut(s) 28, 40, 54, 133, 143, 269, 455
CciI TCATGA 1 cut(s) 724
CfoI GCGC 1 cut(s) 267
Cfr10I RCCGGY 9 cut(s) 38, 52, 74, 131, 135, 141, 228, 453, 775
Cfr13I GGNCC 2 cut(s) 322, 746
CpoI CGGWCCG 1 cut(s) 746
CseI GACGC 1 cut(s) 440
CsiI ACCWGGT 1 cut(s) 158
Csp6I GTAC 3 cut(s) 115, 226, 256
CspAI ACCGGT 1 cut(s) 228
CspI CGGWCCG 1 cut(s) 746
CviAII CATG 4 cut(s) 106, 557, 725, 869
CviQI GTAC 3 cut(s) 115, 226, 256
DdeI CTNAG 2 cut(s) 586, 798
DpnI GATC 7 cut(s) 63, 83, 149, 420, 569, 600, 629
DpnII GATC 7 cut(s) 61, 81, 147, 418, 567, 598, 627
DraIII CACNNNGTG 1 cut(s) 162
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
EaeI YGGCCR 4 cut(s) 54, 133, 455, 816
EagI CGGCCG 4 cut(s) 54, 133, 455, 816
Eam1104I CTCTTC 3 cut(s) 216, 387, 503
EarI CTCTTC 3 cut(s) 216, 387, 503
EciI GGCGGA 1 cut(s) 800
Ecl136II GAGCTC 1 cut(s) 244
EclXI CGGCCG 4 cut(s) 54, 133, 455, 816
Eco130I CCWWGG 1 cut(s) 47
Eco24I GRGCYC 2 cut(s) 246, 804
Eco47I GGWCC 2 cut(s) 322, 746
Eco52I CGGCCG 4 cut(s) 54, 133, 455, 816
Eco53kI GAGCTC 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 322
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 158
EcoT14I CCWWGG 1 cut(s) 47
EcoT38I GRGCYC 2 cut(s) 246, 804
ErhI CCWWGG 1 cut(s) 47
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 4 cut(s) 109, 560, 728, 872
FaiI YATR 8 cut(s) 107, 467, 558, 726, 767, 792, 870, 915
FaqI GGGAC 1 cut(s) 335
FatI CATG 4 cut(s) 105, 556, 724, 868
FauI CCCGC 1 cut(s) 871
FbaI TGATCA 2 cut(s) 81, 147
Fnu4HI GCNGC 4 cut(s) 333, 451, 577, 650
FokI GGATG 2 cut(s) 731, 874
FriOI GRGCYC 2 cut(s) 246, 804
FseI GGCCGGCC 1 cut(s) 56
Fsp4HI GCNGC 4 cut(s) 333, 451, 577, 650
GlaI GCGC 1 cut(s) 266
GluI GCNGC 4 cut(s) 333, 451, 577, 650
HaeIII GGCC 9 cut(s) 26, 52, 56, 135, 186, 207, 332, 457, 818
HgaI GACGC 1 cut(s) 440
HhaI GCGC 1 cut(s) 267
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 4 cut(s) 109, 560, 728, 872
Hin6I GCGC 1 cut(s) 265
HinP1I GCGC 1 cut(s) 265
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HinfI GANTC 4 cut(s) 8, 169, 475, 528
HphI GGTGA 3 cut(s) 142, 148, 295
Hpy166II GTNNAC 5 cut(s) 115, 295, 310, 691, 823
Hpy188I TCNGA 7 cut(s) 367, 423, 440, 589, 598, 668, 750
Hpy188III TCNNGA 4 cut(s) 505, 539, 602, 725
Hpy8I GTNNAC 5 cut(s) 115, 295, 310, 691, 823
Hpy99I CGWCG 5 cut(s) 62, 317, 380, 463, 811
HpyAV CCTTC 1 cut(s) 538
HpyCH4III ACNGT 3 cut(s) 154, 292, 307
HpyCH4IV ACGT 2 cut(s) 312, 375
HpyCH4V TGCA 5 cut(s) 4, 165, 202, 234, 576
HpyF10VI GCNNNNNNNGC 5 cut(s) 273, 469, 582, 808, 884
HpyF3I CTNAG 2 cut(s) 586, 798
HpySE526I ACGT 2 cut(s) 312, 375
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 4 cut(s) 109, 560, 728, 872
HspAI GCGC 1 cut(s) 265
Kpn2I TCCGGA 1 cut(s) 601
KroI GCCGGC 5 cut(s) 38, 52, 131, 141, 453
KroNI GCCGGC 5 cut(s) 40, 54, 133, 143, 455
Ksp22I TGATCA 2 cut(s) 81, 147
Kzo9I GATC 7 cut(s) 61, 81, 147, 418, 567, 598, 627
LmnI GCTCC 5 cut(s) 281, 355, 493, 746, 787
Lsp1109I GCAGC 2 cut(s) 437, 588
LweI GCATC 2 cut(s) 309, 896
MabI ACCWGGT 1 cut(s) 158
MaeII ACGT 2 cut(s) 312, 375
MaeIII GTNAC 2 cut(s) 154, 174
MalI GATC 7 cut(s) 63, 83, 149, 420, 569, 600, 629
MboI GATC 7 cut(s) 61, 81, 147, 418, 567, 598, 627
MboII GAAGA 3 cut(s) 233, 404, 520
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 1 cut(s) 627
MhlI GDGCHC 2 cut(s) 246, 804
MluCI AATT 5 cut(s) 12, 18, 284, 338, 550
MmeI TCCRAC 2 cut(s) 89, 588
MroI TCCGGA 1 cut(s) 601
MroNI GCCGGC 5 cut(s) 38, 52, 131, 141, 453
MseI TTAA 1 cut(s) 771
MslI CAYNNNNRTG 2 cut(s) 110, 305
MspR9I CCNGG 1 cut(s) 160
MunI CAATTG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 434
MvaI CCWGG 1 cut(s) 160
MvnI CGCG 1 cut(s) 878
MwoI GCNNNNNNNGC 5 cut(s) 273, 469, 582, 808, 884
NaeI GCCGGC 5 cut(s) 40, 54, 133, 143, 455
NdeII GATC 7 cut(s) 61, 81, 147, 418, 567, 598, 627
NgoMIV GCCGGC 5 cut(s) 38, 52, 131, 141, 453
NlaIII CATG 4 cut(s) 109, 560, 728, 872
NlaIV GGNNCC 5 cut(s) 140, 277, 323, 357, 629
NmuCI GTSAC 1 cut(s) 154
PagI TCATGA 1 cut(s) 724
PcsI WCGNNNNNNNCGW 1 cut(s) 303
PctI GAATGC 1 cut(s) 434
PdiI GCCGGC 5 cut(s) 40, 54, 133, 143, 455
PfeI GAWTC 4 cut(s) 8, 169, 475, 528
PflFI GACNNNGTC 1 cut(s) 314
PinAI ACCGGT 1 cut(s) 228
PkrI GCNGC 4 cut(s) 334, 452, 578, 651
PpuMI RGGWCCY 1 cut(s) 322
Psp124BI GAGCTC 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 158
PspGI CCWGG 1 cut(s) 158
PspN4I GGNNCC 5 cut(s) 140, 277, 323, 357, 629
PspPI GGNCC 2 cut(s) 322, 746
PspPPI RGGWCCY 1 cut(s) 322
PsuI RGATCY 1 cut(s) 627
PsyI GACNNNGTC 1 cut(s) 314
RigI GGCCGGCC 1 cut(s) 56
RsaI GTAC 3 cut(s) 116, 227, 257
RsaNI GTAC 3 cut(s) 115, 226, 256
RseI CAYNNNNRTG 2 cut(s) 110, 305
Rsr2I CGGWCCG 1 cut(s) 746
RsrII CGGWCCG 1 cut(s) 746
SacI GAGCTC 1 cut(s) 246
SaqAI TTAA 1 cut(s) 771
SatI GCNGC 4 cut(s) 333, 451, 577, 650
Sau3AI GATC 7 cut(s) 61, 81, 147, 418, 567, 598, 627
Sau96I GGNCC 2 cut(s) 322, 746
ScrFI CCNGG 1 cut(s) 160
SduI GDGCHC 2 cut(s) 246, 804
SetI ASST 9 cut(s) 89, 161, 246, 315, 327, 378, 402, 846, 850
SexAI ACCWGGT 1 cut(s) 158
SfaNI GCATC 2 cut(s) 309, 896
SinI GGWCC 2 cut(s) 322, 746
SmiMI CAYNNNNRTG 2 cut(s) 110, 305
SmlI CTYRAG 2 cut(s) 245, 326
SmoI CTYRAG 2 cut(s) 245, 326
Sse9I AATT 5 cut(s) 12, 18, 284, 338, 550
SsiI CCGC 4 cut(s) 333, 649, 785, 878
SstI GAGCTC 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 47
TaaI ACNGT 3 cut(s) 154, 292, 307
TaiI ACGT 2 cut(s) 315, 378
TaqI TCGA 3 cut(s) 485, 570, 864
TaqII GACCGA 1 cut(s) 851
TasI AATT 5 cut(s) 12, 18, 284, 338, 550
TatI WGTACW 1 cut(s) 114
TauI GCSGC 2 cut(s) 335, 652
TfiI GAWTC 4 cut(s) 8, 169, 475, 528
Tru1I TTAA 1 cut(s) 771
Tru9I TTAA 1 cut(s) 771
TscAI CASTG 2 cut(s) 117, 662
TseFI GTSAC 1 cut(s) 154
TseI GCWGC 2 cut(s) 450, 576
Tsp45I GTSAC 1 cut(s) 154
TspDTI ATGAA 2 cut(s) 94, 713
TspGWI ACGGA 2 cut(s) 368, 771
TspRI CASTG 2 cut(s) 117, 662
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 322, 746
XapI RAATTY 3 cut(s) 18, 338, 550
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.