Rh4BG328500

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014723
Physical Location & Seq
Forward (+)
49433850 .. 49435493
1644 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG328500.1

Sequence Viewer

Length: 972 bp
ATGCATGATGCAATTGGAATTCCTGCTTGTTTTTCGGCCGGTGTTACTCATCAAAGGCCGGTTGATCATGATCCGGCGGCGGTGATCAGGTCAGGACAGAGTGTGTGTATGTCTGTTTATAGTACCAAGATTGCTGGTCAGTGTCGTTTGATCACTATTACTTGGTGCAAGAATCTTCTGCTACATGGCCTCTCTGTTTCTGTTCAAAGCCAAGAAGGAGAGGATGACTATAGATGCAAGGTTGAGCTGAAGCCCTGGTACTTTTGGAGGAAGACAGGCGCCAAACAATTCATGGTGGATGGGATCACAGTGGATGTTGTCTGGGACCTAAAAGCTGCAAAGTTCAATGGCGAAACCGAGCCACAGTCGGACTATTATGTTGCCATTGTGTGTGAAGAAGAAGTGGTGTTGCTTGTTGGTGATCAGAAGAAGGATGCATATAGAAGAACAGGTTGCAGACCATCACTGATTGAACCAATCTTGGTTTCAAGGAAGGAGCATTTGTTTGGTAAGAGGAAGTACTCAACAAGAATCAAGTTTCATGAAAAGGAAAAGGGAAAGGGAAAGGGAAAGTTTCATGAGATTTTAATTGAGTGCAACAACAACAACAACAATTCTGTAACCGGGTCCGGGTCTGATCCAGAATTGGAGATAAAGATTGATGGCAACCAAGCCATTCATGTGAAGCACCTTCAATGGAAATTCAGAGGAAATGAGTCGGTTGATGTGAACAAAACCAAAGTTGAAGTCTATTATGATGTTCATGACTGGCTCTTTAGCTCTGGTCCAAGGTACGGAATGTTCATATTCAAGCCAATACCATCAGAATCTGCAGAGTGTTCTTTATCAACAACAACCCCAGTTTTGACTAATCATCAAAAGTCAAGGTCTGGATTAGAAGATGAGGATGATGATAATGCAGGTGGGTCATCTGGGTTTTCCTTATTGGTTTATGCTTGGAAAGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

323

Amino Acids

36.37

Weight (kDa)

6.6

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 31 - 321 3.2e-103 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014723)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25740
malus_domestica MD13G1099400.v1.1 MD16G1100500.v1.1
prunus_persica Prupe.1G246500_v2.0.a1
pyrus_communis pycom13g08680 pycom16g08520
rosa_chinensis RchiOBHm_Chr4g0433181
rosa_laevigata RLG00000006776
rosa_multiflora Rmu_ssc0000295.1_g000012
rosa_roxburghii Rroxscaffold_5G00374680
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320300 Rh4BG328500 Rh4CG343900 Rh4DG323900
rosa_wichuraiana Rw0G008710 Rw4G027810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 911
Acc36I ACCTGC 1 cut(s) 911
AccB1I GGYRCC 1 cut(s) 278
AciI CCGC 2 cut(s) 77, 80
AclWI GGATC 3 cut(s) 65, 311, 632
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 18, 701
AcuI CTGAAG 1 cut(s) 269
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 124, 260, 521, 794
AfiI CCNNNNNNNGG 2 cut(s) 630, 794
AgsI TTSAA 7 cut(s) 206, 346, 473, 489, 695, 746, 811
AjnI CCWGG 1 cut(s) 254
AjuI GAANNNNNNNTTGG 2 cut(s) 469, 501
AluBI AGCT 3 cut(s) 247, 335, 780
AluI AGCT 3 cut(s) 247, 335, 780
AlwI GGATC 3 cut(s) 65, 311, 632
AlwNI CAGNNNCTG 1 cut(s) 830
AoxI GGCC 3 cut(s) 36, 56, 187
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 2 cut(s) 18, 701
ArsI GACNNNNNNTTYG 2 cut(s) 732, 764
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 325, 627, 785
AsuC2I CCSGG 2 cut(s) 625, 631
AsuHPI GGTGA 2 cut(s) 94, 431
AvaII GGWCC 3 cut(s) 325, 627, 785
BanI GGYRCC 1 cut(s) 278
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BbsI GAAGAC 1 cut(s) 278
BbvI GCAGC 1 cut(s) 322
BccI CCATC 4 cut(s) 293, 469, 656, 829
BciT130I CCWGG 1 cut(s) 256
BclI TGATCA 4 cut(s) 64, 84, 150, 421
BcnI CCSGG 2 cut(s) 625, 631
BfmI CTRYAG 2 cut(s) 229, 831
BfoI RGCGCY 1 cut(s) 282
BfuAI ACCTGC 1 cut(s) 911
BisI GCNGC 2 cut(s) 78, 336
BlsI GCNGC 2 cut(s) 79, 337
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 3 cut(s) 256, 625, 631
Bme18I GGWCC 3 cut(s) 325, 627, 785
BmgT120I GGNCC 3 cut(s) 325, 627, 785
BmiI GGNNCC 3 cut(s) 280, 326, 628
BmrFI CCNGG 3 cut(s) 256, 625, 631
BmrI ACTGGG 1 cut(s) 854
BmsI GCATC 2 cut(s) 224, 424
BmuI ACTGGG 1 cut(s) 854
BpiI GAAGAC 1 cut(s) 278
BpuMI CCSGG 2 cut(s) 625, 631
BsaBI GATNNNNATC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 254, 788
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 2 cut(s) 630, 794
Bse118I RCCGGY 2 cut(s) 38, 58
Bse1I ACTGG 2 cut(s) 773, 860
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 2 cut(s) 254, 788
BseGI GGATG 5 cut(s) 229, 304, 319, 439, 913
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 2 cut(s) 630, 794
BseNI ACTGG 2 cut(s) 773, 860
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 322
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 3 cut(s) 38, 58, 189
BshNI GGYRCC 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 39
BsiSI CCGG 5 cut(s) 39, 59, 74, 624, 630
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 2 cut(s) 630, 794
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 3 cut(s) 38, 58, 189
Bsp143I GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 637
BspACI CCGC 2 cut(s) 77, 80
BspANI GGCC 3 cut(s) 38, 58, 189
BspHI TCATGA 4 cut(s) 67, 541, 577, 763
BspLI GGNNCC 3 cut(s) 280, 326, 628
BspMAI CTGCAG 1 cut(s) 835
BspMI ACCTGC 1 cut(s) 911
BspPI GGATC 3 cut(s) 65, 311, 632
BspT107I GGYRCC 1 cut(s) 278
BsrFI RCCGGY 2 cut(s) 38, 58
BsrI ACTGG 2 cut(s) 773, 860
BssAI RCCGGY 2 cut(s) 38, 58
BssECI CCNNGG 2 cut(s) 254, 788
BssMI GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 637
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 788
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 2 cut(s) 310, 366
BstACI GRCGYC 1 cut(s) 279
BstF5I GGATG 5 cut(s) 229, 304, 319, 439, 913
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 7 cut(s) 67, 73, 87, 153, 306, 424, 640
BstMBI GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 637
BstMCI CGRYCG 1 cut(s) 39
BstNI CCWGG 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 254, 623, 629
BstSFI CTRYAG 2 cut(s) 229, 831
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 278
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 3 cut(s) 38, 58, 189
BtsCI GGATG 5 cut(s) 229, 304, 319, 439, 913
BtsIMutI CAGTG 3 cut(s) 146, 315, 464
BveI ACCTGC 1 cut(s) 911
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 4 cut(s) 67, 541, 577, 763
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 2 cut(s) 38, 58
Cfr13I GGNCC 3 cut(s) 325, 627, 785
Csp6I GTAC 4 cut(s) 123, 259, 520, 793
CviAII CATG 8 cut(s) 5, 68, 185, 292, 542, 578, 680, 764
CviQI GTAC 4 cut(s) 123, 259, 520, 793
DinI GGCGCC 1 cut(s) 280
DpnI GATC 7 cut(s) 66, 72, 86, 152, 305, 423, 639
DpnII GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 637
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 788
Eco47I GGWCC 3 cut(s) 325, 627, 785
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 788
EcoT22I ATGCAT 2 cut(s) 6, 439
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 788
FaeI CATG 8 cut(s) 8, 71, 188, 295, 545, 581, 683, 767
FaqI GGGAC 1 cut(s) 338
FatI CATG 8 cut(s) 4, 67, 184, 291, 541, 577, 679, 763
FbaI TGATCA 4 cut(s) 64, 84, 150, 421
Fnu4HI GCNGC 2 cut(s) 78, 336
FokI GGATG 5 cut(s) 236, 311, 326, 446, 920
Fsp4HI GCNGC 2 cut(s) 78, 336
GlaI GCGC 1 cut(s) 280
GluI GCNGC 2 cut(s) 78, 336
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 3 cut(s) 38, 58, 189
HapII CCGG 5 cut(s) 39, 59, 74, 624, 630
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 8 cut(s) 8, 71, 188, 295, 545, 581, 683, 767
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 4 cut(s) 172, 531, 716, 827
HpaII CCGG 5 cut(s) 39, 59, 74, 624, 630
HphI GGTGA 2 cut(s) 94, 431
Hpy166II GTNNAC 1 cut(s) 730
Hpy188I TCNGA 5 cut(s) 370, 426, 637, 707, 826
Hpy188III TCNNGA 7 cut(s) 68, 93, 542, 578, 641, 764, 891
Hpy8I GTNNAC 1 cut(s) 730
HpyAV CCTTC 4 cut(s) 209, 424, 487, 701
HpyCH4III ACNGT 2 cut(s) 310, 366
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 8 cut(s) 8, 71, 188, 295, 545, 581, 683, 767
HspAI GCGC 1 cut(s) 279
KasI GGCGCC 1 cut(s) 278
Ksp22I TGATCA 4 cut(s) 64, 84, 150, 421
Kzo9I GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 637
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 2 cut(s) 224, 424
MaeIII GTNAC 2 cut(s) 43, 619
MalI GATC 7 cut(s) 66, 72, 86, 152, 305, 423, 639
MboI GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 637
MboII GAAGA 7 cut(s) 167, 283, 407, 410, 439, 456, 911
MfeI CAATTG 1 cut(s) 12
MluCI AATT 7 cut(s) 12, 18, 287, 588, 613, 644, 701
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 1 cut(s) 725
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 6 cut(s) 200, 214, 261, 507, 701, 898
Mph1103I ATGCAT 2 cut(s) 6, 439
MseI TTAA 1 cut(s) 587
MslI CAYNNNNRTG 1 cut(s) 680
MspI CCGG 5 cut(s) 39, 59, 74, 624, 630
MspR9I CCNGG 3 cut(s) 256, 625, 631
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 256
NarI GGCGCC 1 cut(s) 279
NciI CCSGG 2 cut(s) 625, 631
NdeII GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 637
NlaIII CATG 8 cut(s) 8, 71, 188, 295, 545, 581, 683, 767
NlaIV GGNNCC 3 cut(s) 280, 326, 628
NsiI ATGCAT 2 cut(s) 6, 439
PagI TCATGA 4 cut(s) 67, 541, 577, 763
PaqCI CACCTGC 1 cut(s) 911
PfeI GAWTC 3 cut(s) 172, 531, 827
PkrI GCNGC 2 cut(s) 79, 337
PleI GAGTC 1 cut(s) 724
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 724
PpuMI RGGWCCY 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 3 cut(s) 280, 326, 628
PspPI GGNCC 3 cut(s) 325, 627, 785
PspPPI RGGWCCY 1 cut(s) 325
PsrI GAACNNNNNNTAC 2 cut(s) 785, 817
PstI CTGCAG 1 cut(s) 835
PstNI CAGNNNCTG 1 cut(s) 830
RsaI GTAC 4 cut(s) 124, 260, 521, 794
RsaNI GTAC 4 cut(s) 123, 259, 520, 793
RseI CAYNNNNRTG 1 cut(s) 680
SaqAI TTAA 1 cut(s) 587
SatI GCNGC 2 cut(s) 78, 336
Sau3AI GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 637
Sau96I GGNCC 3 cut(s) 325, 627, 785
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 1 cut(s) 725
ScrFI CCNGG 3 cut(s) 256, 625, 631
SfaNI GCATC 2 cut(s) 224, 424
SfcI CTRYAG 2 cut(s) 229, 831
SfoI GGCGCC 1 cut(s) 280
SinI GGWCC 3 cut(s) 325, 627, 785
SmiMI CAYNNNNRTG 1 cut(s) 680
Sse9I AATT 7 cut(s) 12, 18, 287, 588, 613, 644, 701
SsiI CCGC 2 cut(s) 77, 80
SspDI GGCGCC 1 cut(s) 278
StyD4I CCNGG 3 cut(s) 254, 623, 629
StyI CCWWGG 1 cut(s) 788
TaaI ACNGT 2 cut(s) 310, 366
TasI AATT 7 cut(s) 12, 18, 287, 588, 613, 644, 701
TatI WGTACW 1 cut(s) 519
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 3 cut(s) 172, 531, 827
Tru1I TTAA 1 cut(s) 587
Tru9I TTAA 1 cut(s) 587
TscAI CASTG 3 cut(s) 146, 315, 471
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 7 cut(s) 280, 530, 558, 566, 668, 752, 793
TspGWI ACGGA 1 cut(s) 810
TspRI CASTG 3 cut(s) 146, 315, 471
VpaK11BI GGWCC 3 cut(s) 325, 627, 785
XapI RAATTY 2 cut(s) 18, 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
ZrmI AGTACT 1 cut(s) 521
Zsp2I ATGCAT 2 cut(s) 6, 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.