MD13G1099400.v1.1

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014723
Physical Location & Seq
Reverse (-)
7022493 .. 7023341
849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1099400.v1.1.491

Sequence Viewer

Length: 849 bp
ATGCATGATTCAATTGGAATTCCGCCCTGCTTCTCAGCCGGCACTCATCCCACCCCAGCCGGCGAGATCAGGTCGGGGCAGAGCATGTTCATGTCAGTGTACAGCACGAAGATAGCCGGCCAGTGTCGGCTGATCACCGTCAACTGGTGCAAGAATCTGTTACTCCACGGCCTCTCTGTTTCGGTGCAAGGCCAAAACAGAGACGAAGAGTGTCGATGCAAAATCGAGCTCAAGCCGTGGTACTTCTGGCGCAAGCAAGGCTCCAAGCAATTCACGGTGGACGGCAGCGCCGTGGATGTCGTCTGGGACCTCAAAGCCGCGAAATTCAACGGCGAAACGGAGCCACAATCGGACTACTACGTCGCCATTGTCTGCGAAGACGAGGTGGTTTTGCTCGTTGGTGATCAGAAGAAAGCCGCATTCAGAAAAACTGCCTGCCGGCCGTCGCTCATCGAACCGATCTTGGTTTCGATGAGGGAGCATTTGTTCGGGAAGAGGAAGTTTTCGACACGAATCAAGTTTCACGAGAAGGAGAATTTCCATGAGATTTTGATCGAGTGCAGCAACGGCTCAGATGGGTCTGATCCGGAGTTGGAGATAAAGATTGATGGGATCCAGGCCGTTCGTGTGAAGCGTCTTCATTGGAAGTTCAGAGGGAATGATTGCGTTGATGTGAACAGAAACAGAGTTGAAGTTTTTTGGGATGTTCATGACTGGCTGTTTTGCTCTGGTCCGAGGCACGGACTGTTCATATTTACGCTGGTTTCGGCGGAGCCTACGGCGGAGAAACCGTCGCTTTCGACCGTTGACCAATCTTTGACTGAAAAGGTATGTTTGTTGGATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

283

Amino Acids

32.02

Weight (kDa)

6.75

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 25 - 262 6.3e-98 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014723)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25740
malus_domestica MD13G1099400.v1.1 MD16G1100500.v1.1
prunus_persica Prupe.1G246500_v2.0.a1
pyrus_communis pycom13g08680 pycom16g08520
rosa_chinensis RchiOBHm_Chr4g0433181
rosa_laevigata RLG00000006776
rosa_multiflora Rmu_ssc0000295.1_g000012
rosa_roxburghii Rroxscaffold_5G00374680
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320300 Rh4BG328500 Rh4CG343900 Rh4DG323900
rosa_wichuraiana Rw0G008710 Rw4G027810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 359
AccII CGCG 1 cut(s) 320
AccIII TCCGGA 1 cut(s) 586
AciI CCGC 5 cut(s) 23, 318, 417, 770, 782
AclWI GGATC 3 cut(s) 578, 607, 620
AcoI YGGCCR 2 cut(s) 118, 440
AcsI RAATTY 3 cut(s) 18, 323, 535
AfaI GTAC 2 cut(s) 101, 242
AfiI CCNNNNNNNGG 2 cut(s) 144, 740
AgsI TTSAA 3 cut(s) 12, 328, 692
AjnI CCWGG 1 cut(s) 615
AloI GAACNNNNNNTCC 2 cut(s) 470, 502
AluBI AGCT 1 cut(s) 229
AluI AGCT 1 cut(s) 229
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 195
AlwI GGATC 3 cut(s) 578, 607, 620
Aor13HI TCCGGA 1 cut(s) 586
AoxI GGCC 5 cut(s) 118, 169, 190, 440, 618
ApeKI GCWGC 2 cut(s) 285, 561
ApoI RAATTY 3 cut(s) 18, 323, 535
AspLEI GCGC 2 cut(s) 252, 290
AspS9I GGNCC 2 cut(s) 307, 731
AsuHPI GGTGA 2 cut(s) 127, 413
AvaII GGWCC 2 cut(s) 307, 731
BamHI GGATCC 1 cut(s) 612
BanII GRGCYC 1 cut(s) 231
BauI CACGAG 1 cut(s) 524
BbsI GAAGAC 2 cut(s) 384, 629
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 2 cut(s) 297, 573
BccI CCATC 2 cut(s) 569, 602
BceAI ACGGC 9 cut(s) 184, 220, 275, 298, 346, 427, 583, 605, 795
BciT130I CCWGG 1 cut(s) 617
BclI TGATCA 2 cut(s) 132, 403
BcoDI GTCTC 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 291
BisI GCNGC 4 cut(s) 286, 318, 417, 562
BlsI GCNGC 4 cut(s) 287, 319, 418, 563
Bme1390I CCNGG 1 cut(s) 617
Bme18I GGWCC 2 cut(s) 307, 731
BmgT120I GGNCC 2 cut(s) 307, 731
BmiI GGNNCC 5 cut(s) 262, 308, 342, 614, 774
BmrFI CCNGG 1 cut(s) 617
BmsI GCATC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 384, 629
BpuEI CTTGAG 1 cut(s) 215
BsaBI GATNNNNATC 1 cut(s) 551
BsaJI CCNNGG 4 cut(s) 166, 236, 291, 734
BsaWI WCCGGW 1 cut(s) 586
BsaXI ACNNNNNCTCC 4 cut(s) 470, 500, 776, 806
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 740
Bse118I RCCGGY 4 cut(s) 38, 59, 116, 438
Bse1I ACTGG 3 cut(s) 121, 149, 719
Bse8I GATNNNNATC 1 cut(s) 551
BseAI TCCGGA 1 cut(s) 586
BseBI CCWGG 1 cut(s) 617
BseDI CCNNGG 4 cut(s) 166, 236, 291, 734
BseGI GGATG 3 cut(s) 46, 301, 709
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 2 cut(s) 144, 740
BseMII CTCAG 2 cut(s) 48, 585
BseNI ACTGG 3 cut(s) 121, 149, 719
BseX3I CGGCCG 1 cut(s) 440
BseXI GCAGC 2 cut(s) 297, 573
BseYI CCCAGC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 580
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 2 cut(s) 443, 804
BshFI GGCC 5 cut(s) 120, 171, 192, 442, 620
BsiEI CGRYCG 2 cut(s) 443, 804
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 5 cut(s) 39, 60, 117, 439, 587
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 2 cut(s) 144, 740
BsmAI GTCTC 1 cut(s) 195
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 5 cut(s) 120, 171, 192, 442, 620
Bsp1286I GDGCHC 1 cut(s) 231
Bsp13I TCCGGA 1 cut(s) 586
Bsp1407I TGTACA 1 cut(s) 99
Bsp143I GATC 7 cut(s) 66, 132, 403, 459, 552, 583, 612
BspACI CCGC 5 cut(s) 23, 318, 417, 770, 782
BspANI GGCC 5 cut(s) 120, 171, 192, 442, 620
BspCNI CTCAG 2 cut(s) 47, 584
BspEI TCCGGA 1 cut(s) 586
BspFNI CGCG 1 cut(s) 320
BspHI TCATGA 1 cut(s) 709
BspLI GGNNCC 5 cut(s) 262, 308, 342, 614, 774
BspPI GGATC 3 cut(s) 578, 607, 620
BsrFI RCCGGY 4 cut(s) 38, 59, 116, 438
BsrGI TGTACA 1 cut(s) 99
BsrI ACTGG 3 cut(s) 121, 149, 719
BssAI RCCGGY 4 cut(s) 38, 59, 116, 438
BssECI CCNNGG 4 cut(s) 166, 236, 291, 734
BssMI GATC 7 cut(s) 66, 132, 403, 459, 552, 583, 612
BssSI CACGAG 1 cut(s) 524
Bst2BI CACGAG 1 cut(s) 524
Bst2UI CCWGG 1 cut(s) 617
Bst4CI ACNGT 5 cut(s) 139, 277, 747, 792, 805
Bst6I CTCTTC 2 cut(s) 201, 488
BstAUI TGTACA 1 cut(s) 99
BstC8I GCNNGC 6 cut(s) 40, 61, 118, 254, 436, 440
BstDEI CTNAG 2 cut(s) 34, 571
BstDSI CCRYGG 3 cut(s) 166, 236, 291
BstF5I GGATG 3 cut(s) 46, 301, 709
BstFNI CGCG 1 cut(s) 320
BstH2I RGCGCY 1 cut(s) 291
BstHHI GCGC 2 cut(s) 252, 290
BstKTI GATC 7 cut(s) 69, 135, 406, 462, 555, 586, 615
BstMAI GTCTC 1 cut(s) 195
BstMBI GATC 7 cut(s) 66, 132, 403, 459, 552, 583, 612
BstMCI CGRYCG 2 cut(s) 443, 804
BstMWI GCNNNNNNNGC 2 cut(s) 258, 567
BstNI CCWGG 1 cut(s) 617
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 615
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 297, 573
BstV2I GAAGAC 2 cut(s) 384, 629
BstX2I RGATCY 1 cut(s) 612
BstYI RGATCY 1 cut(s) 612
BstZI CGGCCG 1 cut(s) 440
BsuRI GGCC 5 cut(s) 120, 171, 192, 442, 620
BtgI CCRYGG 3 cut(s) 166, 236, 291
BtsCI GGATG 3 cut(s) 46, 301, 709
BtsIMutI CAGTG 2 cut(s) 102, 128
Cac8I GCNNGC 6 cut(s) 40, 61, 118, 254, 436, 440
CciI TCATGA 1 cut(s) 709
CfoI GCGC 2 cut(s) 252, 290
Cfr10I RCCGGY 4 cut(s) 38, 59, 116, 438
Cfr13I GGNCC 2 cut(s) 307, 731
CseI GACGC 1 cut(s) 623
Csp6I GTAC 2 cut(s) 100, 241
CviAII CATG 5 cut(s) 5, 85, 91, 542, 710
CviQI GTAC 2 cut(s) 100, 241
DdeI CTNAG 2 cut(s) 34, 571
DpnI GATC 7 cut(s) 68, 134, 405, 461, 554, 585, 614
DpnII GATC 7 cut(s) 66, 132, 403, 459, 552, 583, 612
DrdI GACNNNNNNGTC 1 cut(s) 359
DseDI GACNNNNNNGTC 1 cut(s) 359
EaeI YGGCCR 2 cut(s) 118, 440
EagI CGGCCG 1 cut(s) 440
Eam1104I CTCTTC 2 cut(s) 201, 488
EarI CTCTTC 2 cut(s) 201, 488
EciI GGCGGA 3 cut(s) 12, 785, 797
Ecl136II GAGCTC 1 cut(s) 229
EclXI CGGCCG 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 2 cut(s) 307, 731
Eco52I CGGCCG 1 cut(s) 440
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 615
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 231
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 5 cut(s) 8, 88, 94, 545, 713
FaiI YATR 7 cut(s) 6, 86, 92, 543, 711, 752, 832
FaqI GGGAC 1 cut(s) 320
FatI CATG 5 cut(s) 4, 84, 90, 541, 709
FbaI TGATCA 2 cut(s) 132, 403
Fnu4HI GCNGC 4 cut(s) 286, 318, 417, 562
FokI GGATG 3 cut(s) 33, 308, 716
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 4 cut(s) 286, 318, 417, 562
GlaI GCGC 2 cut(s) 251, 289
GluI GCNGC 4 cut(s) 286, 318, 417, 562
GsaI CCCAGC 1 cut(s) 59
HaeII RGCGCY 1 cut(s) 291
HaeIII GGCC 5 cut(s) 120, 171, 192, 442, 620
HapII CCGG 5 cut(s) 39, 60, 117, 439, 587
HgaI GACGC 1 cut(s) 623
HhaI GCGC 2 cut(s) 252, 290
Hin1II CATG 5 cut(s) 8, 88, 94, 545, 713
Hin6I GCGC 2 cut(s) 250, 288
HinP1I GCGC 2 cut(s) 250, 288
HincII GTYRAC 2 cut(s) 142, 808
HindII GTYRAC 2 cut(s) 142, 808
HinfI GANTC 3 cut(s) 8, 154, 513
HpaII CCGG 5 cut(s) 39, 60, 117, 439, 587
HphI GGTGA 2 cut(s) 127, 413
Hpy166II GTNNAC 5 cut(s) 100, 142, 280, 676, 808
Hpy188I TCNGA 7 cut(s) 352, 408, 425, 574, 583, 653, 735
Hpy188III TCNNGA 4 cut(s) 490, 524, 587, 710
Hpy8I GTNNAC 5 cut(s) 100, 142, 280, 676, 808
Hpy99I CGWCG 3 cut(s) 365, 448, 796
HpyAV CCTTC 1 cut(s) 523
HpyCH4III ACNGT 5 cut(s) 139, 277, 747, 792, 805
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 5 cut(s) 4, 150, 187, 219, 561
HpyF10VI GCNNNNNNNGC 2 cut(s) 258, 567
HpyF3I CTNAG 2 cut(s) 34, 571
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 5 cut(s) 8, 88, 94, 545, 713
HspAI GCGC 2 cut(s) 250, 288
Kpn2I TCCGGA 1 cut(s) 586
KroI GCCGGC 4 cut(s) 38, 59, 116, 438
KroNI GCCGGC 4 cut(s) 40, 61, 118, 440
Ksp22I TGATCA 2 cut(s) 132, 403
Kzo9I GATC 7 cut(s) 66, 132, 403, 459, 552, 583, 612
LmnI GCTCC 4 cut(s) 266, 340, 478, 772
Lsp1109I GCAGC 2 cut(s) 297, 573
LweI GCATC 1 cut(s) 206
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 159
MalI GATC 7 cut(s) 68, 134, 405, 461, 554, 585, 614
MboI GATC 7 cut(s) 66, 132, 403, 459, 552, 583, 612
MboII GAAGA 6 cut(s) 121, 218, 389, 421, 505, 629
MfeI CAATTG 1 cut(s) 12
MflI RGATCY 1 cut(s) 612
MhlI GDGCHC 1 cut(s) 231
MluCI AATT 5 cut(s) 12, 18, 269, 323, 535
MmeI TCCRAC 2 cut(s) 573, 819
MnlI CCTC 7 cut(s) 182, 320, 376, 468, 489, 647, 729
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 586
MroNI GCCGGC 4 cut(s) 38, 59, 116, 438
MslI CAYNNNNRTG 2 cut(s) 89, 95
MspI CCGG 5 cut(s) 39, 60, 117, 439, 587
MspR9I CCNGG 1 cut(s) 617
MunI CAATTG 1 cut(s) 12
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 1 cut(s) 617
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 2 cut(s) 258, 567
NaeI GCCGGC 4 cut(s) 40, 61, 118, 440
NdeII GATC 7 cut(s) 66, 132, 403, 459, 552, 583, 612
NgoMIV GCCGGC 4 cut(s) 38, 59, 116, 438
NlaIII CATG 5 cut(s) 8, 88, 94, 545, 713
NlaIV GGNNCC 5 cut(s) 262, 308, 342, 614, 774
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 88
PagI TCATGA 1 cut(s) 709
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 631
PctI GAATGC 1 cut(s) 419
PdiI GCCGGC 4 cut(s) 40, 61, 118, 440
PfeI GAWTC 3 cut(s) 8, 154, 513
PkrI GCNGC 4 cut(s) 287, 319, 418, 563
PpuMI RGGWCCY 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 615
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 1 cut(s) 615
PspN4I GGNNCC 5 cut(s) 262, 308, 342, 614, 774
PspPI GGNCC 2 cut(s) 307, 731
PspPPI RGGWCCY 1 cut(s) 307
PsuI RGATCY 1 cut(s) 612
RsaI GTAC 2 cut(s) 101, 242
RsaNI GTAC 2 cut(s) 100, 241
RseI CAYNNNNRTG 2 cut(s) 89, 95
SacI GAGCTC 1 cut(s) 231
SatI GCNGC 4 cut(s) 286, 318, 417, 562
Sau3AI GATC 7 cut(s) 66, 132, 403, 459, 552, 583, 612
Sau96I GGNCC 2 cut(s) 307, 731
ScrFI CCNGG 1 cut(s) 617
SduI GDGCHC 1 cut(s) 231
SetI ASST 6 cut(s) 74, 231, 312, 363, 387, 831
SfaNI GCATC 1 cut(s) 206
SinI GGWCC 2 cut(s) 307, 731
SmiMI CAYNNNNRTG 2 cut(s) 89, 95
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 5 cut(s) 12, 18, 269, 323, 535
SsiI CCGC 5 cut(s) 23, 318, 417, 770, 782
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 1 cut(s) 615
TaaI ACNGT 5 cut(s) 139, 277, 747, 792, 805
TaiI ACGT 1 cut(s) 363
TaqI TCGA 7 cut(s) 214, 225, 453, 470, 506, 555, 800
TasI AATT 5 cut(s) 12, 18, 269, 323, 535
TatI WGTACW 1 cut(s) 99
TauI GCSGC 2 cut(s) 320, 419
TfiI GAWTC 3 cut(s) 8, 154, 513
TscAI CASTG 2 cut(s) 102, 128
TseI GCWGC 2 cut(s) 285, 561
TspDTI ATGAA 4 cut(s) 79, 629, 698, 739
TspGWI ACGGA 2 cut(s) 353, 756
TspRI CASTG 2 cut(s) 102, 128
VpaK11BI GGWCC 2 cut(s) 307, 731
XapI RAATTY 3 cut(s) 18, 323, 535
XceI RCATGY 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.