Rw4G027810

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014723
Physical Location & Seq
Forward (+)
54525484 .. 54527267
1784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G027810.1

Sequence Viewer

Length: 960 bp
ATGCATGATGCAATTGGAATTCCTGCTTGTTTTTCGGCCGGTGCTACTCATCAAAGGCCGGTTGATCATGATCCGGCGGGGGTGATCAGGTCAGGCCAGAGTGTGTGTAAGTCTGTTTATAGTACCAAGATTGCTGGTCAGTGTCGTTTGATCACTATTACTTGGTGCAAGAATCTTCTGCTACATGGCCTCTCTGTTTCTGTTCAAGGCCAAGAAGGAGAGGATGACTATAGATGCAAGGTTGAGCTGAAGCCCTGGTACTTTTGGAGGAAGACAGGCGCCAAACAATTCATGGTGGATGGGATCACAGTGGATGTTGTCTGGGACCTAAAAGCTGCAAAGTTCAATGGCGAAACCGAACCACAGTCGGACTATTATGTTGCCATTGTGAGTGAAGAAGAGGTGGTGTTGCTTGTTGGTGATCAGAAGAAAGATGCATATAGAAGAACAGGTTGCAGACCATCACTGATTGAACCAATCTTGGTTTCAAGGAAGGAGCATTTGTTTGGTAAGAGGAAGTACTCAACAAGAATCAAGTTTCATGACAAGGGAAAGGGAAAGTTTCATGAGATTTTAATTGAGTGCAACAACAACAACAACAATTCTGTAACCGGGTCCGGGGCTGATCCAGAATTGGAGATAAAGATTGATGGGAACCAAGCCATTCATGTCAAGCACCTTCAGTGGAAATTCAGAGGCAATGAGTCGGTTGATGTGAACAAAACCAAAGTTGAGGTCTATTATGATGTTCATGACTGGCTCTTTAGCTCTGGTCCAAGGTACGGAATGTTCATATTCAAGCCAATACCATCAGAATCTGCAGAGTGTTCTTTATCAACAACAACCCCAGTTTTGACTAATCATCAAAAGTCAAGGTCTGGATTAGAAGATGAGGATGATGATAATGCAGGTGGGTCATCTGGGTTTTCCTTATTGGTTTATGCTTGGAAAGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

319

Amino Acids

35.81

Weight (kDa)

6.6

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 31 - 317 6.4e-104 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014723)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25740
malus_domestica MD13G1099400.v1.1 MD16G1100500.v1.1
prunus_persica Prupe.1G246500_v2.0.a1
pyrus_communis pycom13g08680 pycom16g08520
rosa_chinensis RchiOBHm_Chr4g0433181
rosa_laevigata RLG00000006776
rosa_multiflora Rmu_ssc0000295.1_g000012
rosa_roxburghii Rroxscaffold_5G00374680
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320300 Rh4BG328500 Rh4CG343900 Rh4DG323900
rosa_wichuraiana Rw0G008710 Rw4G027810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 899
Acc36I ACCTGC 1 cut(s) 899
AccB1I GGYRCC 1 cut(s) 278
AciI CCGC 1 cut(s) 77
AclWI GGATC 3 cut(s) 65, 311, 620
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 18, 689
AcuI CTGAAG 2 cut(s) 269, 665
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 124, 260, 521, 782
AfiI CCNNNNNNNGG 2 cut(s) 618, 782
AgsI TTSAA 5 cut(s) 206, 346, 473, 489, 799
AjnI CCWGG 1 cut(s) 254
AjuI GAANNNNNNNTTGG 2 cut(s) 469, 501
AluBI AGCT 3 cut(s) 247, 335, 768
AluI AGCT 3 cut(s) 247, 335, 768
AlwI GGATC 3 cut(s) 65, 311, 620
AlwNI CAGNNNCTG 1 cut(s) 818
AoxI GGCC 5 cut(s) 36, 56, 94, 187, 208
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 2 cut(s) 18, 689
ArsI GACNNNNNNTTYG 2 cut(s) 720, 752
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 325, 615, 773
AsuC2I CCSGG 2 cut(s) 613, 619
AsuHPI GGTGA 2 cut(s) 94, 431
AvaII GGWCC 3 cut(s) 325, 615, 773
BanI GGYRCC 1 cut(s) 278
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BbsI GAAGAC 1 cut(s) 278
BbvI GCAGC 1 cut(s) 322
BccI CCATC 4 cut(s) 293, 469, 644, 817
BciT130I CCWGG 1 cut(s) 256
BclI TGATCA 4 cut(s) 64, 84, 150, 421
BcnI CCSGG 2 cut(s) 613, 619
BfmI CTRYAG 2 cut(s) 229, 819
BfoI RGCGCY 1 cut(s) 282
BfuAI ACCTGC 1 cut(s) 899
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 3 cut(s) 256, 613, 619
Bme18I GGWCC 3 cut(s) 325, 615, 773
BmgT120I GGNCC 3 cut(s) 325, 615, 773
BmiI GGNNCC 4 cut(s) 280, 326, 616, 656
BmrFI CCNGG 3 cut(s) 256, 613, 619
BmrI ACTGGG 1 cut(s) 842
BmsI GCATC 2 cut(s) 224, 424
BmuI ACTGGG 1 cut(s) 842
BpiI GAAGAC 1 cut(s) 278
BpuMI CCSGG 2 cut(s) 613, 619
BsaBI GATNNNNATC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 254, 618, 776
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 2 cut(s) 618, 782
Bse118I RCCGGY 2 cut(s) 38, 58
Bse1I ACTGG 2 cut(s) 761, 848
Bse3DI GCAATG 1 cut(s) 706
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 3 cut(s) 254, 618, 776
BseGI GGATG 4 cut(s) 229, 304, 319, 901
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 2 cut(s) 618, 782
BseMI GCAATG 1 cut(s) 706
BseNI ACTGG 2 cut(s) 761, 848
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 322
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 5 cut(s) 38, 58, 96, 189, 210
BshNI GGYRCC 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 39
BsiSI CCGG 5 cut(s) 39, 59, 74, 612, 618
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 2 cut(s) 618, 782
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 5 cut(s) 38, 58, 96, 189, 210
Bsp143I GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 625
BspACI CCGC 1 cut(s) 77
BspANI GGCC 5 cut(s) 38, 58, 96, 189, 210
BspHI TCATGA 4 cut(s) 67, 541, 565, 751
BspLI GGNNCC 4 cut(s) 280, 326, 616, 656
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 899
BspPI GGATC 3 cut(s) 65, 311, 620
BspT107I GGYRCC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 706
BsrFI RCCGGY 2 cut(s) 38, 58
BsrI ACTGG 2 cut(s) 761, 848
BssAI RCCGGY 2 cut(s) 38, 58
BssECI CCNNGG 3 cut(s) 254, 618, 776
BssMI GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 625
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 776
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 2 cut(s) 310, 366
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 279
BstF5I GGATG 4 cut(s) 229, 304, 319, 901
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 7 cut(s) 67, 73, 87, 153, 306, 424, 628
BstMBI GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 625
BstMCI CGRYCG 1 cut(s) 39
BstNI CCWGG 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 254, 611, 617
BstSFI CTRYAG 2 cut(s) 229, 819
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 278
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 5 cut(s) 38, 58, 96, 189, 210
BtsCI GGATG 4 cut(s) 229, 304, 319, 901
BtsIMutI CAGTG 4 cut(s) 146, 315, 464, 689
BveI ACCTGC 1 cut(s) 899
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 4 cut(s) 67, 541, 565, 751
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 2 cut(s) 38, 58
Cfr13I GGNCC 3 cut(s) 325, 615, 773
Csp6I GTAC 4 cut(s) 123, 259, 520, 781
CviAII CATG 8 cut(s) 5, 68, 185, 292, 542, 566, 668, 752
CviQI GTAC 4 cut(s) 123, 259, 520, 781
DinI GGCGCC 1 cut(s) 280
DpnI GATC 7 cut(s) 66, 72, 86, 152, 305, 423, 627
DpnII GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 625
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 776
Eco47I GGWCC 3 cut(s) 325, 615, 773
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 2 cut(s) 269, 665
EcoO109I RGGNCCY 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 776
EcoT22I ATGCAT 2 cut(s) 6, 439
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 776
FaeI CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 671, 755
FaqI GGGAC 1 cut(s) 338
FatI CATG 8 cut(s) 4, 67, 184, 291, 541, 565, 667, 751
FauI CCCGC 1 cut(s) 70
FbaI TGATCA 4 cut(s) 64, 84, 150, 421
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 4 cut(s) 236, 311, 326, 908
Fsp4HI GCNGC 1 cut(s) 336
GlaI GCGC 1 cut(s) 280
GluI GCNGC 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 5 cut(s) 38, 58, 96, 189, 210
HapII CCGG 5 cut(s) 39, 59, 74, 612, 618
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 671, 755
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 4 cut(s) 172, 531, 704, 815
HpaII CCGG 5 cut(s) 39, 59, 74, 612, 618
HphI GGTGA 2 cut(s) 94, 431
Hpy166II GTNNAC 1 cut(s) 718
Hpy188I TCNGA 4 cut(s) 370, 426, 695, 814
Hpy188III TCNNGA 6 cut(s) 68, 542, 566, 629, 752, 879
Hpy8I GTNNAC 1 cut(s) 718
HpyAV CCTTC 3 cut(s) 209, 487, 689
HpyCH4III ACNGT 2 cut(s) 310, 366
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 671, 755
HspAI GCGC 1 cut(s) 279
KasI GGCGCC 1 cut(s) 278
Ksp22I TGATCA 4 cut(s) 64, 84, 150, 421
Kzo9I GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 625
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 2 cut(s) 224, 424
MaeIII GTNAC 1 cut(s) 607
MalI GATC 7 cut(s) 66, 72, 86, 152, 305, 423, 627
MboI GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 625
MboII GAAGA 7 cut(s) 167, 283, 407, 410, 439, 456, 899
MfeI CAATTG 1 cut(s) 12
MluCI AATT 7 cut(s) 12, 18, 287, 576, 601, 632, 689
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 8 cut(s) 200, 214, 261, 394, 507, 689, 727, 886
Mph1103I ATGCAT 2 cut(s) 6, 439
MseI TTAA 1 cut(s) 575
MspI CCGG 5 cut(s) 39, 59, 74, 612, 618
MspR9I CCNGG 3 cut(s) 256, 613, 619
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 256
NarI GGCGCC 1 cut(s) 279
NciI CCSGG 2 cut(s) 613, 619
NdeII GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 625
NlaIII CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 671, 755
NlaIV GGNNCC 4 cut(s) 280, 326, 616, 656
NsiI ATGCAT 2 cut(s) 6, 439
PagI TCATGA 4 cut(s) 67, 541, 565, 751
PaqCI CACCTGC 1 cut(s) 899
PfeI GAWTC 3 cut(s) 172, 531, 815
PkrI GCNGC 1 cut(s) 337
PleI GAGTC 1 cut(s) 712
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 712
PpuMI RGGWCCY 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 4 cut(s) 280, 326, 616, 656
PspPI GGNCC 3 cut(s) 325, 615, 773
PspPPI RGGWCCY 1 cut(s) 325
PsrI GAACNNNNNNTAC 2 cut(s) 773, 805
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 818
RsaI GTAC 4 cut(s) 124, 260, 521, 782
RsaNI GTAC 4 cut(s) 123, 259, 520, 781
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 7 cut(s) 64, 70, 84, 150, 303, 421, 625
Sau96I GGNCC 3 cut(s) 325, 615, 773
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 3 cut(s) 256, 613, 619
SfaNI GCATC 2 cut(s) 224, 424
SfcI CTRYAG 2 cut(s) 229, 819
SfoI GGCGCC 1 cut(s) 280
SinI GGWCC 3 cut(s) 325, 615, 773
Sse9I AATT 7 cut(s) 12, 18, 287, 576, 601, 632, 689
SsiI CCGC 1 cut(s) 77
SspDI GGCGCC 1 cut(s) 278
StyD4I CCNGG 3 cut(s) 254, 611, 617
StyI CCWWGG 1 cut(s) 776
TaaI ACNGT 2 cut(s) 310, 366
TasI AATT 7 cut(s) 12, 18, 287, 576, 601, 632, 689
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 3 cut(s) 172, 531, 815
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 4 cut(s) 146, 315, 471, 689
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 6 cut(s) 280, 530, 554, 656, 740, 781
TspGWI ACGGA 1 cut(s) 798
TspRI CASTG 4 cut(s) 146, 315, 471, 689
VpaK11BI GGWCC 3 cut(s) 325, 615, 773
XapI RAATTY 2 cut(s) 18, 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
ZrmI AGTACT 1 cut(s) 521
Zsp2I ATGCAT 2 cut(s) 6, 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.