Rw0G008710

Protein phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00332
OG0014723
Physical Location & Seq
Forward (+)
7977 .. 8939
963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G008710.1

Sequence Viewer

Length: 963 bp
ATGCATGATCCAATTGGAATTCCTGCTTGTTTTTCGGCCGGTGCTACTCATCAAAGGCCGGTTGATCATGATCCGGCGGGAGTGATCAGGTCAGGCCAGAGTGTGTGCAAGTCTGTTTATAGTACCAAGATTGCTGGTCAGTGTCGTTTGATCACTATTACTTGGTGCAAGAATCTTCTGCTACATGGCCTCTCTGTTTCTGTTCAAGGCCAAGAAGGAGAGGATGACTATAGATGCAAGGTTGAGCTGAAGCCCTGGTACTTTTGGAGGAAGACAGGCGCCAAACAATTCATGGTGGATGGGATCACAGTGGATGTTGTCTGGGACCTAAAAGCTGCAAAGTTCAATGGCGAAACCGAGCCACAGTCGGACTATTATGTTGCCATTGTGAGTGAAGAAGAGGTGGTGTTGCTTGTTGGTGATCAGAAGAAAGATGCATATAGAAGAACAGGTTGCAGACCATCACTGATTGAACCAATCTTGGTTTCAAGGAAGGAGCATTTGTTTGGTAAGAGGAAGTACTCAACAAGAATCAAGTTTCATGACAAGGGAAAGGGAAAGTTTCATGAGATTTTAATTGAGTGCAACAACAACAACAACAACAATTCTGTAACCGGGTCCGGGGCTGATCCAGAATTGGAGATAAAGATTGATGGGAACCAAGCCATTCATGTCAAGCACCTTCAGTGGAAATTCAGAGGCAATGAGTCAGTTGATGTGAACAAAACCAAAGTTGAGGTCTATTATGATGTTCATGACTGGCTCTTTAGCTCTGGTCCAAGGTACGGAATGTTCATATTCAAGCCAATACCATCAGAATCTGCAGAGTGTTCTTTATCAACAACAACCCCAGTTTTGACTAATCATCAAAAGTCAAGGTCTGGATTAGAAGATGAGGATGATGATAATGCAGGTGGGTCATCTGGGTTTTCCTTATTGGTTTATGCTTGGAAAGTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868 PP2C

Protein Analysis

320

Amino Acids

35.95

Weight (kDa)

6.6

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 31 - 318 7.8e-104 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014723)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g25740
malus_domestica MD13G1099400.v1.1 MD16G1100500.v1.1
prunus_persica Prupe.1G246500_v2.0.a1
pyrus_communis pycom13g08680 pycom16g08520
rosa_chinensis RchiOBHm_Chr4g0433181
rosa_laevigata RLG00000006776
rosa_multiflora Rmu_ssc0000295.1_g000012
rosa_roxburghii Rroxscaffold_5G00374680
rosa_rugosa Rorug04G0266500
rosa_samantha Rh4AG320300 Rh4BG328500 Rh4CG343900 Rh4DG323900
rosa_wichuraiana Rw0G008710 Rw4G027810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 902
Acc36I ACCTGC 1 cut(s) 902
AccB1I GGYRCC 1 cut(s) 278
AciI CCGC 1 cut(s) 77
AclWI GGATC 4 cut(s) 2, 65, 311, 623
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 2 cut(s) 18, 692
AcuI CTGAAG 2 cut(s) 269, 668
AcyI GRCGYC 1 cut(s) 279
AfaI GTAC 4 cut(s) 124, 260, 521, 785
AfiI CCNNNNNNNGG 2 cut(s) 621, 785
AgsI TTSAA 5 cut(s) 206, 346, 473, 489, 802
AjnI CCWGG 1 cut(s) 254
AjuI GAANNNNNNNTTGG 2 cut(s) 469, 501
AluBI AGCT 3 cut(s) 247, 335, 771
AluI AGCT 3 cut(s) 247, 335, 771
AlwI GGATC 4 cut(s) 2, 65, 311, 623
AlwNI CAGNNNCTG 1 cut(s) 821
AoxI GGCC 5 cut(s) 36, 56, 94, 187, 208
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 2 cut(s) 18, 692
ArsI GACNNNNNNTTYG 2 cut(s) 723, 755
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 3 cut(s) 325, 618, 776
AsuC2I CCSGG 2 cut(s) 616, 622
AsuHPI GGTGA 1 cut(s) 431
AvaII GGWCC 3 cut(s) 325, 618, 776
BanI GGYRCC 1 cut(s) 278
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BbsI GAAGAC 1 cut(s) 278
BbvI GCAGC 1 cut(s) 322
BccI CCATC 4 cut(s) 293, 469, 647, 820
BciT130I CCWGG 1 cut(s) 256
BclI TGATCA 4 cut(s) 64, 84, 150, 421
BcnI CCSGG 2 cut(s) 616, 622
BfmI CTRYAG 2 cut(s) 229, 822
BfoI RGCGCY 1 cut(s) 282
BfuAI ACCTGC 1 cut(s) 902
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 3 cut(s) 256, 616, 622
Bme18I GGWCC 3 cut(s) 325, 618, 776
BmgT120I GGNCC 3 cut(s) 325, 618, 776
BmiI GGNNCC 4 cut(s) 280, 326, 619, 659
BmrFI CCNGG 3 cut(s) 256, 616, 622
BmrI ACTGGG 1 cut(s) 845
BmsI GCATC 2 cut(s) 224, 424
BmuI ACTGGG 1 cut(s) 845
BpiI GAAGAC 1 cut(s) 278
BpuMI CCSGG 2 cut(s) 616, 622
BsaBI GATNNNNATC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 279
BsaJI CCNNGG 3 cut(s) 254, 621, 779
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 2 cut(s) 621, 785
Bse118I RCCGGY 2 cut(s) 38, 58
Bse1I ACTGG 2 cut(s) 764, 851
Bse3DI GCAATG 1 cut(s) 709
Bse8I GATNNNNATC 1 cut(s) 69
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 3 cut(s) 254, 621, 779
BseGI GGATG 4 cut(s) 229, 304, 319, 904
BseJI GATNNNNATC 1 cut(s) 69
BseLI CCNNNNNNNGG 2 cut(s) 621, 785
BseMI GCAATG 1 cut(s) 709
BseNI ACTGG 2 cut(s) 764, 851
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 322
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 5 cut(s) 38, 58, 96, 189, 210
BshNI GGYRCC 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 39
BsiSI CCGG 5 cut(s) 39, 59, 74, 615, 621
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 2 cut(s) 621, 785
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 5 cut(s) 38, 58, 96, 189, 210
Bsp143I GATC 8 cut(s) 7, 64, 70, 84, 150, 303, 421, 628
BspACI CCGC 1 cut(s) 77
BspANI GGCC 5 cut(s) 38, 58, 96, 189, 210
BspHI TCATGA 4 cut(s) 67, 541, 565, 754
BspLI GGNNCC 4 cut(s) 280, 326, 619, 659
BspMAI CTGCAG 1 cut(s) 826
BspMI ACCTGC 1 cut(s) 902
BspPI GGATC 4 cut(s) 2, 65, 311, 623
BspT107I GGYRCC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 709
BsrFI RCCGGY 2 cut(s) 38, 58
BsrI ACTGG 2 cut(s) 764, 851
BssAI RCCGGY 2 cut(s) 38, 58
BssECI CCNNGG 3 cut(s) 254, 621, 779
BssMI GATC 8 cut(s) 7, 64, 70, 84, 150, 303, 421, 628
BssNI GRCGYC 1 cut(s) 279
BssT1I CCWWGG 1 cut(s) 779
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 2 cut(s) 310, 366
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 279
BstF5I GGATG 4 cut(s) 229, 304, 319, 904
BstH2I RGCGCY 1 cut(s) 282
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 8 cut(s) 10, 67, 73, 87, 153, 306, 424, 631
BstMBI GATC 8 cut(s) 7, 64, 70, 84, 150, 303, 421, 628
BstMCI CGRYCG 1 cut(s) 39
BstNI CCWGG 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 254, 614, 620
BstSFI CTRYAG 2 cut(s) 229, 822
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 278
BstZI CGGCCG 1 cut(s) 36
BsuRI GGCC 5 cut(s) 38, 58, 96, 189, 210
BtsCI GGATG 4 cut(s) 229, 304, 319, 904
BtsIMutI CAGTG 4 cut(s) 146, 315, 464, 692
BveI ACCTGC 1 cut(s) 902
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 4 cut(s) 67, 541, 565, 754
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 2 cut(s) 38, 58
Cfr13I GGNCC 3 cut(s) 325, 618, 776
Csp6I GTAC 4 cut(s) 123, 259, 520, 784
CviAII CATG 8 cut(s) 5, 68, 185, 292, 542, 566, 671, 755
CviQI GTAC 4 cut(s) 123, 259, 520, 784
DinI GGCGCC 1 cut(s) 280
DpnI GATC 8 cut(s) 9, 66, 72, 86, 152, 305, 423, 630
DpnII GATC 8 cut(s) 7, 64, 70, 84, 150, 303, 421, 628
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 779
Eco47I GGWCC 3 cut(s) 325, 618, 776
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 2 cut(s) 269, 668
EcoO109I RGGNCCY 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 779
EcoT22I ATGCAT 2 cut(s) 6, 439
EgeI GGCGCC 1 cut(s) 280
EheI GGCGCC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 779
FaeI CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 674, 758
FaqI GGGAC 1 cut(s) 338
FatI CATG 8 cut(s) 4, 67, 184, 291, 541, 565, 670, 754
FauI CCCGC 1 cut(s) 70
FbaI TGATCA 4 cut(s) 64, 84, 150, 421
Fnu4HI GCNGC 1 cut(s) 336
FokI GGATG 4 cut(s) 236, 311, 326, 911
Fsp4HI GCNGC 1 cut(s) 336
GlaI GCGC 1 cut(s) 280
GluI GCNGC 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 282
HaeIII GGCC 5 cut(s) 38, 58, 96, 189, 210
HapII CCGG 5 cut(s) 39, 59, 74, 615, 621
HhaI GCGC 1 cut(s) 281
Hin1I GRCGYC 1 cut(s) 279
Hin1II CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 674, 758
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 4 cut(s) 172, 531, 707, 818
HpaII CCGG 5 cut(s) 39, 59, 74, 615, 621
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 721
Hpy188I TCNGA 4 cut(s) 370, 426, 698, 817
Hpy188III TCNNGA 6 cut(s) 68, 542, 566, 632, 755, 882
Hpy8I GTNNAC 1 cut(s) 721
HpyAV CCTTC 3 cut(s) 209, 487, 692
HpyCH4III ACNGT 2 cut(s) 310, 366
Hsp92I GRCGYC 1 cut(s) 279
Hsp92II CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 674, 758
HspAI GCGC 1 cut(s) 279
KasI GGCGCC 1 cut(s) 278
Ksp22I TGATCA 4 cut(s) 64, 84, 150, 421
Kzo9I GATC 8 cut(s) 7, 64, 70, 84, 150, 303, 421, 628
LmnI GCTCC 1 cut(s) 496
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 2 cut(s) 224, 424
MaeIII GTNAC 1 cut(s) 610
MalI GATC 8 cut(s) 9, 66, 72, 86, 152, 305, 423, 630
MboI GATC 8 cut(s) 7, 64, 70, 84, 150, 303, 421, 628
MboII GAAGA 7 cut(s) 167, 283, 407, 410, 439, 456, 902
MfeI CAATTG 1 cut(s) 12
MluCI AATT 7 cut(s) 12, 18, 287, 576, 604, 635, 692
Mly113I GGCGCC 1 cut(s) 279
MlyI GAGTC 1 cut(s) 716
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 8 cut(s) 200, 214, 261, 394, 507, 692, 730, 889
Mph1103I ATGCAT 2 cut(s) 6, 439
MseI TTAA 1 cut(s) 575
MspI CCGG 5 cut(s) 39, 59, 74, 615, 621
MspR9I CCNGG 3 cut(s) 256, 616, 622
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 1 cut(s) 256
NarI GGCGCC 1 cut(s) 279
NciI CCSGG 2 cut(s) 616, 622
NdeII GATC 8 cut(s) 7, 64, 70, 84, 150, 303, 421, 628
NlaIII CATG 8 cut(s) 8, 71, 188, 295, 545, 569, 674, 758
NlaIV GGNNCC 4 cut(s) 280, 326, 619, 659
NsiI ATGCAT 2 cut(s) 6, 439
PagI TCATGA 4 cut(s) 67, 541, 565, 754
PaqCI CACCTGC 1 cut(s) 902
PfeI GAWTC 3 cut(s) 172, 531, 818
PkrI GCNGC 1 cut(s) 337
PleI GAGTC 1 cut(s) 715
PluTI GGCGCC 1 cut(s) 282
PpsI GAGTC 1 cut(s) 715
PpuMI RGGWCCY 1 cut(s) 325
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 4 cut(s) 280, 326, 619, 659
PspPI GGNCC 3 cut(s) 325, 618, 776
PspPPI RGGWCCY 1 cut(s) 325
PsrI GAACNNNNNNTAC 2 cut(s) 776, 808
PstI CTGCAG 1 cut(s) 826
PstNI CAGNNNCTG 1 cut(s) 821
RsaI GTAC 4 cut(s) 124, 260, 521, 785
RsaNI GTAC 4 cut(s) 123, 259, 520, 784
SaqAI TTAA 1 cut(s) 575
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 8 cut(s) 7, 64, 70, 84, 150, 303, 421, 628
Sau96I GGNCC 3 cut(s) 325, 618, 776
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 1 cut(s) 716
ScrFI CCNGG 3 cut(s) 256, 616, 622
SfaNI GCATC 2 cut(s) 224, 424
SfcI CTRYAG 2 cut(s) 229, 822
SfoI GGCGCC 1 cut(s) 280
SinI GGWCC 3 cut(s) 325, 618, 776
Sse9I AATT 7 cut(s) 12, 18, 287, 576, 604, 635, 692
SsiI CCGC 1 cut(s) 77
SspDI GGCGCC 1 cut(s) 278
StyD4I CCNGG 3 cut(s) 254, 614, 620
StyI CCWWGG 1 cut(s) 779
TaaI ACNGT 2 cut(s) 310, 366
TasI AATT 7 cut(s) 12, 18, 287, 576, 604, 635, 692
TatI WGTACW 1 cut(s) 519
TfiI GAWTC 3 cut(s) 172, 531, 818
Tru1I TTAA 1 cut(s) 575
Tru9I TTAA 1 cut(s) 575
TscAI CASTG 4 cut(s) 146, 315, 471, 692
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 6 cut(s) 280, 530, 554, 659, 743, 784
TspGWI ACGGA 1 cut(s) 801
TspRI CASTG 4 cut(s) 146, 315, 471, 692
VpaK11BI GGWCC 3 cut(s) 325, 618, 776
XapI RAATTY 2 cut(s) 18, 692
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
ZrmI AGTACT 1 cut(s) 521
Zsp2I ATGCAT 2 cut(s) 6, 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.