Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0012236

Physical Location & Seq

Genomic Coordinates Forward (+)

27546763 .. 27550780

Gene Structure 4018 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g25930.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1923 bp

ATGAAGAAAATCTCTGTCTTGAGTTTTCTGTGCTTGTTCCTCGGTTTTCATAGCCAGAGGGTATCATCACAAGAACATGCTAGCACCCCACCCAGAGGTTGGAATTCGTACGACTCCTTTTGTTGGACTATTTCCGAGGAAGAGTACTTGGCAAGTGCTGATGTCATAGCTAAGCAGCTACTTCCACATGGATATGAGTATGCTGTTGTGGATTACCTGTGGTATAGGAGAAAGGTTCCAGGCGCTTATGTTGATTCTCTTGGATTTGATGTAATCGATAAATGGGGGAGGCCTATACCTGACCCAGGTAGGTGGCCTTCCTCCAAAGGTGGGAATGGGTTCACTGAAGTGGCATCAAAAGTACATCAGATGGGTTTAAAGTTTGGGATTCATCTCATGAGAGGAATTAGTACTCAGGCAGTAAATGCGAATACCCCTATCTTCGATGTCACCACGGGGACTGCTTATAAAGAGTCTGGAAGAATATGGACTGCAAAAGACATAGCGATCACAGCACGGCGTTGCGCCTGGATGCAAAATGGTTTCATGAGTGTGAATACTACATTGGGTGCTGGGAAAGCCTTCTTAAAATCTCTCTATGTACAGTATGACGAGTGGGGTGTTGATTTTGTGAAAAATGATTGTGTGTTTGGTGAAGACATAGATGTTGGTGAAATAACCGTTGTGTCGGAGGTTCTAAAGCAATTTGACCATCCTGTGGTGTATTCTCTCTCGCCTGGAACCCAAGTAACACCTGCTCTGGCCAAAGGTCTAACTGGTATAGTCAATATGTACCGTATAACAGGGGATGATTGGGACACATGGGGCGATGTAGCAGCCCATTTTGATGTTACCAGGGATTTTGCTGCTGCGAATTTGATAGGAACAAACGGCTTGCTGGGAAAATCATGGCCTGACTTAGATATGCTTCCCCTGGGATGGCTTACCGATCCAGGTTCGAATGATGGTCCACACAGACAAACGAAACTCACTTTAGACGAGCAAAGAACTCAGATGACTTTGTGGTCTATTGCCAAATCTCCTCTCATGTTTGGAGGGGATGTGAGAAAGCTTGATGCTGCCACAACTAGTCTCCTCACAAATCCTACCTTGCTGGAGATTAATTCGTTTAGCTCCAACAATAAGGAGTTTCCATTGATTTCAGGTACTTCAAGTAAAGGGGACATCGAGAATGATGATCTGGTTAACTCCTGGCAATCAAGAAGACAATTAAAAAGTTTACACACTTCAGATTCACAGGTTCTTCATCTCACTAGCTGTGCAGATGAGAAAGCAATTGGGTGGTCCACTAAAGCTCTTGACCAAGATCTTGAACACATCTGCTGGAAAGAACACTCGAGAAAGAAATACCAGACACCTTTTTGCTTAAACAAGAGAGAATCTCTTTTGGCAGTAGAAGAAGACATAATGTACAAACAGCAATACCAGGGGAAGGTCAATTTGTTAGCTAGTGACAAGGAGGACATGTGCTTGCATGGTTCTCCGAAAAGAAAGCTCACATCAAAAGAGCTCAAGAGAGGTTTCTTTTCACCTTGCGGATGGGATGCAAATCAGATGTGGGAGTTGAACCCCAATGGAACCTTGTCAAATAGCTATTCGGGCCTATGTGCGACAGTAAATTCTGTTAAAGGTAAAGCCAATTCTGTAGGAGTTCGTGCTTGGATCGCCACAGGAAGAAATGGAGAAATATATGTAGCCTTTTTCAATCTGAACTCAGCAAAAACTACCATAACTGCACAAAAATCAGACCTGGCGAAAGCACTTCCAGGCTCGAGAGTAAGTGCAGGTCCCTGCAAAGCCACAGAAGTATGGAGCGGACAAGACTTGGGGATTATAGACCAGGCCATATCATTTGGTGTAGAAACTCATGGGACTGCCCTATTTGTCCTACATTGTGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005994 GO:0005995 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006672 GO:0006677 GO:0006687 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009987 GO:0015925 GO:0016042 GO:0016052 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019377 GO:0030149 GO:0034641 GO:0043603 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044262 GO:0044275 GO:0044424 GO:0044464 GO:0046352 GO:0046466 GO:0046477 GO:0046479 GO:0046514 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1903509

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Melibiase_2

Protein Analysis

641

Amino Acids

70.71

Weight (kDa)

6.45

Isoelectric Point (pI)

28.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	203 - 375	1.1e-10	Alpha galactosidase A
Melibiase_C	PF17801	558 - 637	1.8e-09	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26380 AT3G26380
fragaria_vesca	FvH4_4g25930
malus_domestica	MD13G1098300.v1.1 MD13G1098400.v1.1 MD16G1099400.v1.1
prunus_persica	Prupe.1G247000_v2.0.a1
pyrus_communis	pycom13g08600 pycom16g08460
rosa_chinensis	RchiOBHm_Chr4g0433401
rosa_laevigata	RLG00000006756
rosa_multiflora	Rmu_sc0000759.1_g000006
rosa_roxburghii	Rroxscaffold_5G00374950
rosa_rugosa	Rorug04G0268500
rosa_samantha	Rh4AG323300 Rh4BG331000 Rh4CG346300 Rh4DG327400
rosa_wichuraiana	Rw4G028000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	468
AarI	CACCTGC	1 cut(s)	763
AasI	GACNNNNNNGTC	1 cut(s)	1024
Acc36I	ACCTGC	2 cut(s)	763, 1796
AccB7I	CCANNNNNTGG	2 cut(s)	99, 718
AccBSI	CCGCTC	1 cut(s)	1836
AciI	CCGC	2 cut(s)	1557, 1836
AclWI	GGATC	2 cut(s)	944, 1691
AcoI	YGGCCR	1 cut(s)	762
AcsI	RAATTY	3 cut(s)	103, 874, 1639
AcuI	CTGAAG	2 cut(s)	366, 1233
AfaI	GTAC	8 cut(s)	110, 146, 363, 412, 603, 794, 1168, 1433
AfiI	CCNNNNNNNGG	8 cut(s)	95, 99, 123, 305, 330, 718, 939, 1453
AflIII	ACRYGT	1 cut(s)	1485
AgsI	TTSAA	4 cut(s)	1173, 1334, 1588, 1726
AhlI	ACTAGT	1 cut(s)	1088
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
AleI	CACNNNNGTG	1 cut(s)	347
Alw21I	GWGCWC	1 cut(s)	1533
Alw26I	GTCTC	1 cut(s)	1097
AlwI	GGATC	2 cut(s)	944, 1691
Ama87I	CYCGRG	2 cut(s)	1357, 1792
AoxI	GGCC	6 cut(s)	290, 314, 762, 911, 1621, 1863
ApeKI	GCWGC	5 cut(s)	175, 836, 866, 869, 1079
ApoI	RAATTY	3 cut(s)	103, 874, 1639
AseI	ATTAAT	1 cut(s)	1122
Asp700I	GAANNNNTTC	2 cut(s)	338, 581
AspLEI	GCGC	2 cut(s)	245, 527
AspS9I	GGNCC	4 cut(s)	968, 1305, 1621, 1808
AsuHPI	GGTGA	4 cut(s)	442, 665, 683, 1542
AsuII	TTCGAA	1 cut(s)	959
AsuNHI	GCTAGC	1 cut(s)	80
AvaI	CYCGRG	2 cut(s)	1357, 1792
AvaII	GGWCC	3 cut(s)	968, 1305, 1808
BalI	TGGCCA	1 cut(s)	764
BanII	GRGCYC	1 cut(s)	1533
BarI	GAAGNNNNNNTAC	2 cut(s)	301, 333
BbsI	GAAGAC	3 cut(s)	663, 1231, 1428
Bbv12I	GWGCWC	1 cut(s)	1533
BbvI	GCAGC	5 cut(s)	187, 848, 853, 856, 1066
BccI	CCATC	5 cut(s)	364, 720, 933, 959, 1554
BceAI	ACGGC	2 cut(s)	533, 907
BcoDI	GTCTC	1 cut(s)	1097
BcuI	ACTAGT	1 cut(s)	1088
BfaI	CTAG	4 cut(s)	81, 1089, 1275, 1470
BfmI	CTRYAG	1 cut(s)	1665
BfoI	RGCGCY	1 cut(s)	246
BfuAI	ACCTGC	2 cut(s)	763, 1796
BglII	AGATCT	1 cut(s)	1327
BisI	GCNGC	5 cut(s)	176, 837, 867, 870, 1080
BlpI	GCTNAGC	1 cut(s)	171
BlsI	GCNGC	5 cut(s)	177, 838, 868, 871, 1081
BmcAI	AGTACT	2 cut(s)	146, 412
Bme18I	GGWCC	3 cut(s)	968, 1305, 1808
BmeT110I	CYCGRG	2 cut(s)	1357, 1792
BmgT120I	GGNCC	4 cut(s)	968, 1305, 1621, 1808
BmiI	GGNNCC	4 cut(s)	237, 742, 1600, 1810
BmsI	GCATC	4 cut(s)	362, 522, 1066, 1555
BmtI	GCTAGC	1 cut(s)	84
BpiI	GAAGAC	3 cut(s)	663, 1231, 1428
BplI	GAGNNNNNCTC	2 cut(s)	1387, 1419
BpmI	CTGGAG	1 cut(s)	1136
Bpu1102I	GCTNAGC	1 cut(s)	171
Bpu14I	TTCGAA	1 cut(s)	959
BpuEI	CTTGAG	2 cut(s)	40, 1517
Bsa29I	ATCGAT	1 cut(s)	276
BsaBI	GATNNNNATC	1 cut(s)	1569
BsaJI	CCNNGG	8 cut(s)	40, 135, 304, 453, 855, 933, 934, 1447
BsaXI	ACNNNNNCTCC	4 cut(s)	1047, 1077, 1472, 1502
Bsc4I	CCNNNNNNNGG	8 cut(s)	95, 99, 123, 305, 330, 718, 939, 1453
Bse1I	ACTGG	1 cut(s)	781
Bse8I	GATNNNNATC	1 cut(s)	1569
BseCI	ATCGAT	1 cut(s)	276
BseDI	CCNNGG	8 cut(s)	40, 135, 304, 453, 855, 933, 934, 1447
BseGI	GGATG	7 cut(s)	537, 712, 814, 944, 1066, 1565, 1570
BseJI	GATNNNNATC	1 cut(s)	1569
BseLI	CCNNNNNNNGG	8 cut(s)	95, 99, 123, 305, 330, 718, 939, 1453
BseMII	CTCAG	3 cut(s)	428, 1025, 1749
BseNI	ACTGG	1 cut(s)	781
BseRI	GAGGAG	2 cut(s)	1032, 1085
BseXI	GCAGC	5 cut(s)	187, 848, 853, 856, 1066
BseYI	CCCAGC	2 cut(s)	572, 898
BsgI	GTGCAG	3 cut(s)	1302, 1740, 1824
BshFI	GGCC	6 cut(s)	292, 316, 764, 913, 1623, 1865
BshVI	ATCGAT	1 cut(s)	276
BsiHKAI	GWGCWC	1 cut(s)	1533
BsiHKCI	CYCGRG	2 cut(s)	1357, 1792
BsiWI	CGTACG	1 cut(s)	108
BslFI	GGGAC	5 cut(s)	472, 830, 1196, 1794, 1906
BslI	CCNNNNNNNGG	8 cut(s)	95, 99, 123, 305, 330, 718, 939, 1453
BsmAI	GTCTC	1 cut(s)	1097
BsmFI	GGGAC	5 cut(s)	472, 830, 1196, 1794, 1906
BsnI	GGCC	6 cut(s)	292, 316, 764, 913, 1623, 1865
BsoBI	CYCGRG	2 cut(s)	1357, 1792
Bsp119I	TTCGAA	1 cut(s)	959
Bsp1286I	GDGCHC	1 cut(s)	1533
Bsp1407I	TGTACA	2 cut(s)	601, 1431
Bsp143I	GATC	5 cut(s)	507, 949, 1198, 1327, 1683
Bsp1720I	GCTNAGC	1 cut(s)	171
BspACI	CCGC	2 cut(s)	1557, 1836
BspANI	GGCC	6 cut(s)	292, 316, 764, 913, 1623, 1865
BspCNI	CTCAG	3 cut(s)	427, 1024, 1748
BspDI	ATCGAT	1 cut(s)	276
BspHI	TCATGA	2 cut(s)	396, 546
BspLI	GGNNCC	4 cut(s)	237, 742, 1600, 1810
BspMI	ACCTGC	2 cut(s)	763, 1796
BspOI	GCTAGC	1 cut(s)	84
BspPI	GGATC	2 cut(s)	944, 1691
BspT104I	TTCGAA	1 cut(s)	959
BsrBI	CCGCTC	1 cut(s)	1836
BsrGI	TGTACA	2 cut(s)	601, 1431
BsrI	ACTGG	1 cut(s)	781
BssECI	CCNNGG	8 cut(s)	40, 135, 304, 453, 855, 933, 934, 1447
BssMI	GATC	5 cut(s)	507, 949, 1198, 1327, 1683
Bst4CI	ACNGT	4 cut(s)	606, 682, 797, 1636
Bst6I	CTCTTC	1 cut(s)	135
BstAPI	GCANNNNNTGC	1 cut(s)	425
BstAUI	TGTACA	2 cut(s)	601, 1431
BstBI	TTCGAA	1 cut(s)	959
BstC8I	GCNNGC	3 cut(s)	82, 896, 1493
BstDEI	CTNAG	5 cut(s)	171, 414, 919, 1011, 1735
BstDSI	CCRYGG	1 cut(s)	453
BstENI	CCTNNNNNAGG	1 cut(s)	303
BstF5I	GGATG	7 cut(s)	537, 712, 814, 944, 1066, 1565, 1570
BstH2I	RGCGCY	1 cut(s)	246
BstHHI	GCGC	2 cut(s)	245, 527
BstKTI	GATC	5 cut(s)	510, 952, 1201, 1330, 1686
BstMAI	GTCTC	1 cut(s)	1097
BstMBI	GATC	5 cut(s)	507, 949, 1198, 1327, 1683
BstMWI	GCNNNNNNNGC	5 cut(s)	425, 512, 578, 1620, 1685
BstNSI	RCATGY	2 cut(s)	80, 1489
BstSFI	CTRYAG	1 cut(s)	1665
BstV1I	GCAGC	5 cut(s)	187, 848, 853, 856, 1066
BstV2I	GAAGAC	3 cut(s)	663, 1231, 1428
BstX2I	RGATCY	1 cut(s)	1327
BstXI	CCANNNNNNTGG	1 cut(s)	312
BstYI	RGATCY	1 cut(s)	1327
Bsu15I	ATCGAT	1 cut(s)	276
BsuRI	GGCC	6 cut(s)	292, 316, 764, 913, 1623, 1865
BsuTUI	ATCGAT	1 cut(s)	276
BtgI	CCRYGG	1 cut(s)	453
BtgZI	GCGATG	1 cut(s)	843
BtsCI	GGATG	7 cut(s)	537, 712, 814, 944, 1066, 1565, 1570
BtsIMutI	CAGTG	1 cut(s)	342
BveI	ACCTGC	2 cut(s)	763, 1796
Cac8I	GCNNGC	3 cut(s)	82, 896, 1493
CciI	TCATGA	2 cut(s)	396, 546
CfoI	GCGC	2 cut(s)	245, 527
Cfr13I	GGNCC	4 cut(s)	968, 1305, 1621, 1808
ClaI	ATCGAT	1 cut(s)	276
Csp6I	GTAC	8 cut(s)	109, 145, 362, 411, 602, 793, 1167, 1432
CviQI	GTAC	8 cut(s)	109, 145, 362, 411, 602, 793, 1167, 1432
DdeI	CTNAG	5 cut(s)	171, 414, 919, 1011, 1735
DpnI	GATC	5 cut(s)	509, 951, 1200, 1329, 1685
DpnII	GATC	5 cut(s)	507, 949, 1198, 1327, 1683
DraI	TTTAAA	1 cut(s)	378
DrdI	GACNNNNNNGTC	1 cut(s)	1024
DseDI	GACNNNNNNGTC	1 cut(s)	1024
EaeI	YGGCCR	1 cut(s)	762
Eam1104I	CTCTTC	1 cut(s)	135
EarI	CTCTTC	1 cut(s)	135
Ecl136II	GAGCTC	1 cut(s)	1531
Eco147I	AGGCCT	1 cut(s)	292
Eco24I	GRGCYC	1 cut(s)	1533
Eco47I	GGWCC	3 cut(s)	968, 1305, 1808
Eco53kI	GAGCTC	1 cut(s)	1531
Eco57I	CTGAAG	2 cut(s)	366, 1233
Eco88I	CYCGRG	2 cut(s)	1357, 1792
EcoICRI	GAGCTC	1 cut(s)	1531
EcoNI	CCTNNNNNAGG	1 cut(s)	303
EcoO109I	RGGNCCY	1 cut(s)	1808
EcoRI	GAATTC	1 cut(s)	103
EcoT38I	GRGCYC	1 cut(s)	1533
FaqI	GGGAC	5 cut(s)	472, 830, 1196, 1794, 1906
Fnu4HI	GCNGC	5 cut(s)	176, 837, 867, 870, 1080
FokI	GGATG	7 cut(s)	544, 699, 821, 951, 1073, 1572, 1577
FriOI	GRGCYC	1 cut(s)	1533
Fsp4HI	GCNGC	5 cut(s)	176, 837, 867, 870, 1080
FspBI	CTAG	4 cut(s)	81, 1089, 1275, 1470
GlaI	GCGC	2 cut(s)	244, 526
GluI	GCNGC	5 cut(s)	176, 837, 867, 870, 1080
GsaI	CCCAGC	2 cut(s)	576, 902
GsuI	CTGGAG	1 cut(s)	1136
HaeII	RGCGCY	1 cut(s)	246
HaeIII	GGCC	6 cut(s)	292, 316, 764, 913, 1623, 1865
HhaI	GCGC	2 cut(s)	245, 527
Hin6I	GCGC	2 cut(s)	243, 525
HinP1I	GCGC	2 cut(s)	243, 525
HincII	GTYRAC	1 cut(s)	1207
HindII	GTYRAC	1 cut(s)	1207
HindIII	AAGCTT	1 cut(s)	1070
HinfI	GANTC	6 cut(s)	113, 254, 388, 473, 1253, 1400
HpaI	GTTAAC	1 cut(s)	1207
HphI	GGTGA	4 cut(s)	442, 665, 683, 1542
Hpy166II	GTNNAC	5 cut(s)	342, 971, 1207, 1241, 1308
Hpy188I	TCNGA	9 cut(s)	136, 369, 691, 1014, 1252, 1506, 1575, 1731, 1768
Hpy8I	GTNNAC	5 cut(s)	342, 971, 1207, 1241, 1308
HpyAV	CCTTC	3 cut(s)	327, 592, 1447
HpyCH4III	ACNGT	4 cut(s)	606, 682, 797, 1636
HpyCH4V	TGCA	8 cut(s)	494, 535, 1283, 1495, 1568, 1757, 1805, 1815
HpyF10VI	GCNNNNNNNGC	5 cut(s)	425, 512, 578, 1620, 1685
HpyF3I	CTNAG	5 cut(s)	171, 414, 919, 1011, 1735
HspAI	GCGC	2 cut(s)	243, 525
KspAI	GTTAAC	1 cut(s)	1207
Kzo9I	GATC	5 cut(s)	507, 949, 1198, 1327, 1683
LmnI	GCTCC	2 cut(s)	1139, 1833
Lsp1109I	GCAGC	5 cut(s)	187, 848, 853, 856, 1066
LweI	GCATC	4 cut(s)	362, 522, 1066, 1555
MaeI	CTAG	4 cut(s)	81, 1089, 1275, 1470
MaeIII	GTNAC	4 cut(s)	448, 748, 850, 1472
MalI	GATC	5 cut(s)	509, 951, 1200, 1329, 1685
MbiI	CCGCTC	1 cut(s)	1836
MboI	GATC	5 cut(s)	507, 949, 1198, 1327, 1683
MfeI	CAATTG	1 cut(s)	1296
MflI	RGATCY	1 cut(s)	1327
MhlI	GDGCHC	1 cut(s)	1533
MlsI	TGGCCA	1 cut(s)	764
MluNI	TGGCCA	1 cut(s)	764
MlyI	GAGTC	2 cut(s)	107, 482
MmeI	TCCRAC	4 cut(s)	80, 104, 669, 1161
Mox20I	TGGCCA	1 cut(s)	764
MroXI	GAANNNNTTC	2 cut(s)	338, 581
MscI	TGGCCA	1 cut(s)	764
MseI	TTAA	7 cut(s)	377, 587, 1122, 1206, 1232, 1388, 1647
MslI	CAYNNNNRTG	4 cut(s)	192, 347, 551, 846
Msp20I	TGGCCA	1 cut(s)	764
MunI	CAATTG	1 cut(s)	1296
MwoI	GCNNNNNNNGC	5 cut(s)	425, 512, 578, 1620, 1685
NdeII	GATC	5 cut(s)	507, 949, 1198, 1327, 1683
NheI	GCTAGC	1 cut(s)	80
NlaIV	GGNNCC	4 cut(s)	237, 742, 1600, 1810
NmuCI	GTSAC	2 cut(s)	448, 1472
NspI	RCATGY	2 cut(s)	80, 1489
NspV	TTCGAA	1 cut(s)	959
OliI	CACNNNNGTG	1 cut(s)	347
PaeR7I	CTCGAG	2 cut(s)	1357, 1792
PagI	TCATGA	2 cut(s)	396, 546
PaqCI	CACCTGC	1 cut(s)	763
PasI	CCCWGGG	1 cut(s)	934
PceI	AGGCCT	1 cut(s)	292
PciI	ACATGT	1 cut(s)	1485
PdmI	GAANNNNTTC	2 cut(s)	338, 581
PfeI	GAWTC	4 cut(s)	254, 388, 1253, 1400
Pfl23II	CGTACG	1 cut(s)	108
PflMI	CCANNNNNTGG	2 cut(s)	99, 718
PkrI	GCNGC	5 cut(s)	177, 838, 868, 871, 1081
PleI	GAGTC	2 cut(s)	107, 481
PpsI	GAGTC	2 cut(s)	107, 481
PpuMI	RGGWCCY	1 cut(s)	1808
PscI	ACATGT	1 cut(s)	1485
PshBI	ATTAAT	1 cut(s)	1122
PsiI	TTATAA	1 cut(s)	468
Psp124BI	GAGCTC	1 cut(s)	1533
Psp5II	RGGWCCY	1 cut(s)	1808
PspFI	CCCAGC	2 cut(s)	572, 898
PspLI	CGTACG	1 cut(s)	108
PspN4I	GGNNCC	4 cut(s)	237, 742, 1600, 1810
PspPI	GGNCC	4 cut(s)	968, 1305, 1621, 1808
PspPPI	RGGWCCY	1 cut(s)	1808
PsuI	RGATCY	1 cut(s)	1327
RsaI	GTAC	8 cut(s)	110, 146, 363, 412, 603, 794, 1168, 1433
RsaNI	GTAC	8 cut(s)	109, 145, 362, 411, 602, 793, 1167, 1432
RseI	CAYNNNNRTG	4 cut(s)	192, 347, 551, 846
SacI	GAGCTC	1 cut(s)	1533
SaqAI	TTAA	7 cut(s)	377, 587, 1122, 1206, 1232, 1388, 1647
SatI	GCNGC	5 cut(s)	176, 837, 867, 870, 1080
Sau3AI	GATC	5 cut(s)	507, 949, 1198, 1327, 1683
Sau96I	GGNCC	4 cut(s)	968, 1305, 1621, 1808
ScaI	AGTACT	2 cut(s)	146, 412
SchI	GAGTC	2 cut(s)	107, 482
SduI	GDGCHC	1 cut(s)	1533
SfaNI	GCATC	4 cut(s)	362, 522, 1066, 1555
SfcI	CTRYAG	1 cut(s)	1665
Sfr274I	CTCGAG	2 cut(s)	1357, 1792
SfuI	TTCGAA	1 cut(s)	959
SinI	GGWCC	3 cut(s)	968, 1305, 1808
SlaI	CTCGAG	2 cut(s)	1357, 1792
SmiMI	CAYNNNNRTG	4 cut(s)	192, 347, 551, 846
SmlI	CTYRAG	4 cut(s)	19, 1357, 1532, 1792
SmoI	CTYRAG	4 cut(s)	19, 1357, 1532, 1792
SpeI	ACTAGT	1 cut(s)	1088
SseBI	AGGCCT	1 cut(s)	292
SsiI	CCGC	2 cut(s)	1557, 1836
SspMI	CTAG	4 cut(s)	81, 1089, 1275, 1470
SstI	GAGCTC	1 cut(s)	1533
StuI	AGGCCT	1 cut(s)	292
TaaI	ACNGT	4 cut(s)	606, 682, 797, 1636
TaqI	TCGA	6 cut(s)	276, 444, 959, 1188, 1358, 1793
TatI	WGTACW	5 cut(s)	144, 361, 410, 601, 1431
TfiI	GAWTC	4 cut(s)	254, 388, 1253, 1400
Tru1I	TTAA	7 cut(s)	377, 587, 1122, 1206, 1232, 1388, 1647
Tru9I	TTAA	7 cut(s)	377, 587, 1122, 1206, 1232, 1388, 1647
TscAI	CASTG	1 cut(s)	349
TseFI	GTSAC	2 cut(s)	448, 1472
TseI	GCWGC	5 cut(s)	175, 836, 866, 869, 1079
Tsp45I	GTSAC	2 cut(s)	448, 1472
TspDTI	ATGAA	5 cut(s)	17, 38, 380, 535, 1256
TspRI	CASTG	1 cut(s)	349
Van91I	CCANNNNNTGG	2 cut(s)	99, 718
VpaK11BI	GGWCC	3 cut(s)	968, 1305, 1808
VspI	ATTAAT	1 cut(s)	1122
XagI	CCTNNNNNAGG	1 cut(s)	303
XapI	RAATTY	3 cut(s)	103, 874, 1639
XceI	RCATGY	2 cut(s)	80, 1489
XcmI	CCANNNNNNNNNTGG	1 cut(s)	96
XhoI	CTCGAG	2 cut(s)	1357, 1792
XmnI	GAANNNNTTC	2 cut(s)	338, 581
XspI	CTAG	4 cut(s)	81, 1089, 1275, 1470
ZrmI	AGTACT	2 cut(s)	146, 412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.