Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012236

Physical Location & Seq

Genomic Coordinates Forward (+)

6969142 .. 6973839

Gene Structure 4698 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1098300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGAATGTCTCCTTGTTCCTCTTCAGCTTTCTCTGCTTCTTACTCGGTCTCTTCAGCCAGAGGGTATCATCACAATATCGCGCTAGGACCCCGCCCAGAGGTTGGAACTCGTATGACTCGTTTATCTGGACTATTTCAGAGGAAGAGTTCTCCACAAATGCCGAAATCATGGCAAAGCAGCTCCTTCCTCATGGATATGAGTATGCTGTGGTGGATTACCTGTGGTATAGGAGAAAAGTTCCAGGCGCTTATGTTGACTCTCTTGGGTTTGATGTGATCGATGAGTGGGGGAGGCCTATACCTGATCCAGTTAGGTGGCCCTCGTCGAGAGGTGGGAAGGGGTTCACTAAAGTGGCAGCAAAAGTACATAGCCTCGGTTTGAAATTCGGGATTCATGTCATGAGAGGAATAAGTACACAGGCAGTAAATGCGAATACCTTTATCTTGGACCCCAACACGGGGACTGCTTACAAAGAGGCTGGAAGAAAATGGACTGCAAAAGATATAGGGATTAAGGAGAGGACATGCGCATGGATGCACAACGGTTTCATGAGTGTGAATACCGAGTTGGGTGCTGGAAAGGCCTTCTTGAGGTCACTCTATAAACAGTATGCTGAATGGGGTGTTGATTTTGTGAAAAATGACTGCGTGTTTGGTGAAGACTTAGATGTACCCGAAATAACCTATATTTCAGAGATTCTGAAGCAACTTGAGCGTCCTATATTGTATTCTCTATCTCCTGGAACCAGAGTAACACCTGCTATGGCCAAGGCTATAAGTGGGATGGTCAACATGTACCGTATAACTGGGGATGATTGGGATTCATGGGGAGATGTTCTAGCCCATTTTGATATAACCAGGGATTTTTCTGCTGCCAATATGATAGGAGCCAAAGGCTTGCTGGGGAAGTCATGGCCTGACTTAGATATGCTCCCGTTGGGATGGCTTAGCGATCCAGGTTCAAATCACGGTCCTCACAGATGGACTAATCTCGGTTTAGGGGAGCAAAGAACACAGATGACTTTGTGGTCTATTGCCAGGTCTCCTCTCATGTTTGGAGGAGATTTGAGAAAACTTCCAGATGAATTAATGAGAATCATCACAAATGACGTCGTGCTGGAGATAGATTCTTTTAGCTCAAATAATAGGGAGTTTTCATACATTACCAGTAATGTGAATGCAAGGGCCGTTAAGAATGATGATCAGTTTCACACCTGGCAATCGAGAAGACATCTGACAAATGCAAAAACTTCAAATACACATGCTTTTGGTCTCACTACCTGTGCAGATGCGAAAGCAATTGGTTGGTCCACTCAATTTCTTGACCGAGATCTTGAACACATCTGCTGGACGGAAAACTCAAGGAGCAAACATCAGGCACCTTTTTGCTTAAACAAGAGAGATTCGCTCTTGGCAATAGATGATGACATAACGGACAAACATCAATATCAGGGGAAGGTCAATTTATTAGCAAGTGATGGAACAGATCGCTGCTTGCATGCTTCTCCTAAACGAAAGGTTACTTCGAAGGACGTTAAGAGAGGTTTCTTTTCACCTTGTAGATGGGATGCGAACCAGATGTGGGAGTTAAACCCCAACGGAACCTTGGTGAATAGTTATTCTGGCCTCTGTGCAAGAATTGATCCTTTTGAAGGCAATAAGATAAAACCTAAGGCCATTAACGATGTTGCCCGCTCTTGGATTGCAACTGGAAGAAAAGGAGAAATATACATAGCGTTTTTCAATTTGCTCGGCAAATGGGCTACTATAACTGCAGAAAGAGCAGACTTAGCCAGAGAACTTCCAATGACAAAACTGGTCAGAGGTTCCTGCGAAGGATCGGAAGTATGGACGGGACGGTACTTAGGGGTAGTAGGCGACGCAATTTCATTTTCGGTTGTACCACACGGGGTTGCACTGATTGTCCTGCACTGTAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005994 GO:0005995 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006672 GO:0006677 GO:0006687 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009987 GO:0015925 GO:0016042 GO:0016052 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019377 GO:0030149 GO:0034641 GO:0043603 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044262 GO:0044275 GO:0044424 GO:0044464 GO:0046352 GO:0046466 GO:0046477 GO:0046479 GO:0046514 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1903509

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Melibiase_2

Protein Analysis

647

Amino Acids

72.65

Weight (kDa)

8.48

Isoelectric Point (pI)

31.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	202 - 375	3e-12	Alpha galactosidase A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26380 AT3G26380
fragaria_vesca	FvH4_4g25930
malus_domestica	MD13G1098300.v1.1 MD13G1098400.v1.1 MD16G1099400.v1.1
prunus_persica	Prupe.1G247000_v2.0.a1
pyrus_communis	pycom13g08600 pycom16g08460
rosa_chinensis	RchiOBHm_Chr4g0433401
rosa_laevigata	RLG00000006756
rosa_multiflora	Rmu_sc0000759.1_g000006
rosa_roxburghii	Rroxscaffold_5G00374950
rosa_rugosa	Rorug04G0268500
rosa_samantha	Rh4AG323300 Rh4BG331000 Rh4CG346300 Rh4DG327400
rosa_wichuraiana	Rw4G028000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	766
AasI	GACNNNNNNGTC	1 cut(s)	1027
AatII	GACGTC	1 cut(s)	1113
Acc16I	TGCGCA	1 cut(s)	529
Acc36I	ACCTGC	1 cut(s)	766
AccB1I	GGYRCC	1 cut(s)	1378
AccB7I	CCANNNNNTGG	1 cut(s)	102
AccBSI	CCGCTC	1 cut(s)	1695
AccII	CGCG	1 cut(s)	81
AciI	CCGC	2 cut(s)	92, 1693
AclWI	GGATC	4 cut(s)	299, 947, 1637, 1846
AcoI	YGGCCR	1 cut(s)	765
AcsI	RAATTY	1 cut(s)	383
AcuI	CTGAAG	3 cut(s)	7, 37, 722
AcyI	GRCGYC	1 cut(s)	1110
AfaI	GTAC	6 cut(s)	366, 415, 672, 797, 1862, 1902
AfiI	CCNNNNNNNGG	9 cut(s)	98, 102, 457, 458, 459, 591, 1582, 1652, 1698
AflIII	ACRYGT	1 cut(s)	792
AgsI	TTSAA	6 cut(s)	382, 963, 1254, 1337, 1652, 1744
AjnI	CCWGG	6 cut(s)	241, 739, 857, 955, 1037, 1214
AjuI	GAANNNNNNNTTGG	2 cut(s)	551, 583
AleI	CACNNNNGTG	1 cut(s)	350
AluBI	AGCT	3 cut(s)	27, 181, 1137
AluI	AGCT	3 cut(s)	27, 181, 1137
Alw26I	GTCTC	4 cut(s)	13, 53, 1047, 1277
AlwI	GGATC	4 cut(s)	299, 947, 1637, 1846
AoxI	GGCC	8 cut(s)	293, 317, 582, 765, 914, 1185, 1624, 1674
ApeKI	GCWGC	4 cut(s)	178, 356, 872, 1491
ApoI	RAATTY	1 cut(s)	383
ArsI	GACNNNNNNTTYG	2 cut(s)	635, 667
AseI	ATTAAT	1 cut(s)	1088
Asp700I	GAANNNNTTC	1 cut(s)	341
AspLEI	GCGC	3 cut(s)	83, 248, 530
AspS9I	GGNCC	6 cut(s)	87, 318, 448, 971, 1185, 1308
AsuHPI	GGTGA	3 cut(s)	668, 1545, 1621
AsuII	TTCGAA	1 cut(s)	1526
AvaII	GGWCC	4 cut(s)	87, 448, 971, 1308
AxyI	CCTNAGG	1 cut(s)	1671
BalI	TGGCCA	1 cut(s)	767
BanI	GGYRCC	1 cut(s)	1378
BbsI	GAAGAC	2 cut(s)	666, 1234
BbvI	GCAGC	4 cut(s)	190, 368, 859, 1478
BccI	CCATC	5 cut(s)	778, 936, 975, 1472, 1557
BceAI	ACGGC	1 cut(s)	1172
BcgI	CGANNNNNNTGC	2 cut(s)	554, 588
BciT130I	CCWGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
BclI	TGATCA	1 cut(s)	1201
BcoDI	GTCTC	4 cut(s)	13, 53, 1047, 1277
BfaI	CTAG	2 cut(s)	84, 839
BfmI	CTRYAG	2 cut(s)	1773, 1933
BfoI	RGCGCY	1 cut(s)	249
BfuAI	ACCTGC	1 cut(s)	766
BglII	AGATCT	1 cut(s)	1330
BisI	GCNGC	4 cut(s)	179, 357, 873, 1492
BlpI	GCTNAGC	1 cut(s)	947
BlsI	GCNGC	4 cut(s)	180, 358, 874, 1493
Bme1390I	CCNGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
Bme18I	GGWCC	4 cut(s)	87, 448, 971, 1308
BmgT120I	GGNCC	6 cut(s)	87, 318, 448, 971, 1185, 1308
BmiI	GGNNCC	7 cut(s)	89, 450, 745, 889, 1380, 1603, 1828
BmrFI	CCNGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
BmrI	ACTGGG	1 cut(s)	816
BmsI	GCATC	3 cut(s)	525, 1279, 1558
BmuI	ACTGGG	1 cut(s)	816
BpiI	GAAGAC	2 cut(s)	666, 1234
BplI	GAGNNNNNCTC	2 cut(s)	1392, 1424
BpmI	CTGGAG	1 cut(s)	1139
Bpu1102I	GCTNAGC	1 cut(s)	947
Bpu14I	TTCGAA	1 cut(s)	1526
BpuEI	CTTGAG	3 cut(s)	610, 731, 1345
Bsa29I	ATCGAT	1 cut(s)	279
BsaHI	GRCGYC	1 cut(s)	1110
BsaI	GGTCTC	3 cut(s)	53, 1047, 1277
BsaJI	CCNNGG	4 cut(s)	373, 768, 858, 1605
Bsc4I	CCNNNNNNNGG	9 cut(s)	98, 102, 457, 458, 459, 591, 1582, 1652, 1698
Bse1I	ACTGG	5 cut(s)	308, 811, 1167, 1714, 1821
Bse21I	CCTNAGG	1 cut(s)	1671
BseBI	CCWGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
BseCI	ATCGAT	1 cut(s)	279
BseDI	CCNNGG	4 cut(s)	373, 768, 858, 1605
BseGI	GGATG	5 cut(s)	540, 789, 817, 947, 1573
BseLI	CCNNNNNNNGG	9 cut(s)	98, 102, 457, 458, 459, 591, 1582, 1652, 1698
BseNI	ACTGG	5 cut(s)	308, 811, 1167, 1714, 1821
BseRI	GAGGAG	2 cut(s)	1035, 1074
BseXI	GCAGC	4 cut(s)	190, 368, 859, 1478
BseYI	CCCAGC	1 cut(s)	901
BsgI	GTGCAG	2 cut(s)	1305, 1913
Bsh1236I	CGCG	1 cut(s)	81
BshFI	GGCC	8 cut(s)	295, 319, 584, 767, 916, 1187, 1626, 1676
BshNI	GGYRCC	1 cut(s)	1378
BshVI	ATCGAT	1 cut(s)	279
BslFI	GGGAC	2 cut(s)	475, 1869
BslI	CCNNNNNNNGG	9 cut(s)	98, 102, 457, 458, 459, 591, 1582, 1652, 1698
BsmAI	GTCTC	4 cut(s)	13, 53, 1047, 1277
BsmFI	GGGAC	2 cut(s)	475, 1869
BsmI	GAATGC	1 cut(s)	1183
BsnI	GGCC	8 cut(s)	295, 319, 584, 767, 916, 1187, 1626, 1676
Bso31I	GGTCTC	3 cut(s)	53, 1047, 1277
Bsp119I	TTCGAA	1 cut(s)	1526
Bsp143I	GATC	8 cut(s)	276, 304, 952, 1201, 1330, 1486, 1642, 1838
Bsp1720I	GCTNAGC	1 cut(s)	947
BspACI	CCGC	2 cut(s)	92, 1693
BspANI	GGCC	8 cut(s)	295, 319, 584, 767, 916, 1187, 1626, 1676
BspDI	ATCGAT	1 cut(s)	279
BspFNI	CGCG	1 cut(s)	81
BspHI	TCATGA	2 cut(s)	399, 549
BspLI	GGNNCC	7 cut(s)	89, 450, 745, 889, 1380, 1603, 1828
BspMAI	CTGCAG	1 cut(s)	1777
BspMI	ACCTGC	1 cut(s)	766
BspPI	GGATC	4 cut(s)	299, 947, 1637, 1846
BspT104I	TTCGAA	1 cut(s)	1526
BspT107I	GGYRCC	1 cut(s)	1378
BspTNI	GGTCTC	3 cut(s)	53, 1047, 1277
BsrBI	CCGCTC	1 cut(s)	1695
BsrI	ACTGG	5 cut(s)	308, 811, 1167, 1714, 1821
BssECI	CCNNGG	4 cut(s)	373, 768, 858, 1605
BssMI	GATC	8 cut(s)	276, 304, 952, 1201, 1330, 1486, 1642, 1838
BssNI	GRCGYC	1 cut(s)	1110
BssT1I	CCWWGG	2 cut(s)	768, 1605
Bst2UI	CCWGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
Bst4CI	ACNGT	6 cut(s)	545, 609, 800, 971, 1860, 1934
Bst6I	CTCTTC	3 cut(s)	26, 56, 138
BstACI	GRCGYC	1 cut(s)	1110
BstAPI	GCANNNNNTGC	1 cut(s)	428
BstBI	TTCGAA	1 cut(s)	1526
BstC8I	GCNNGC	4 cut(s)	899, 1496, 1500, 1693
BstDEI	CTNAG	6 cut(s)	664, 922, 947, 1671, 1789, 1864
BstENI	CCTNNNNNAGG	2 cut(s)	589, 1650
BstF5I	GGATG	5 cut(s)	540, 789, 817, 947, 1573
BstFNI	CGCG	1 cut(s)	81
BstH2I	RGCGCY	1 cut(s)	249
BstHHI	GCGC	3 cut(s)	83, 248, 530
BstKTI	GATC	8 cut(s)	279, 307, 955, 1204, 1333, 1489, 1645, 1841
BstMAI	GTCTC	4 cut(s)	13, 53, 1047, 1277
BstMBI	GATC	8 cut(s)	276, 304, 952, 1201, 1330, 1486, 1642, 1838
BstMWI	GCNNNNNNNGC	6 cut(s)	33, 428, 581, 712, 1781, 1790
BstNI	CCWGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
BstNSI	RCATGY	4 cut(s)	528, 796, 1265, 1502
BstSCI	CCNGG	6 cut(s)	241, 739, 857, 955, 1037, 1214
BstSFI	CTRYAG	2 cut(s)	1773, 1933
BstUI	CGCG	1 cut(s)	81
BstV1I	GCAGC	4 cut(s)	190, 368, 859, 1478
BstV2I	GAAGAC	2 cut(s)	666, 1234
BstX2I	RGATCY	1 cut(s)	1330
BstXI	CCANNNNNNTGG	1 cut(s)	315
BstYI	RGATCY	1 cut(s)	1330
Bsu15I	ATCGAT	1 cut(s)	279
Bsu36I	CCTNAGG	1 cut(s)	1671
BsuRI	GGCC	8 cut(s)	295, 319, 584, 767, 916, 1187, 1626, 1676
BsuTUI	ATCGAT	1 cut(s)	279
BtsCI	GGATG	5 cut(s)	540, 789, 817, 947, 1573
BtsIMutI	CAGTG	2 cut(s)	1916, 1930
BveI	ACCTGC	1 cut(s)	766
Cac8I	GCNNGC	4 cut(s)	899, 1496, 1500, 1693
CciI	TCATGA	2 cut(s)	399, 549
CfoI	GCGC	3 cut(s)	83, 248, 530
Cfr13I	GGNCC	6 cut(s)	87, 318, 448, 971, 1185, 1308
ClaI	ATCGAT	1 cut(s)	279
CseI	GACGC	2 cut(s)	704, 1889
Csp6I	GTAC	6 cut(s)	365, 414, 671, 796, 1861, 1901
CviQI	GTAC	6 cut(s)	365, 414, 671, 796, 1861, 1901
DdeI	CTNAG	6 cut(s)	664, 922, 947, 1671, 1789, 1864
DpnI	GATC	8 cut(s)	278, 306, 954, 1203, 1332, 1488, 1644, 1840
DpnII	GATC	8 cut(s)	276, 304, 952, 1201, 1330, 1486, 1642, 1838
DrdI	GACNNNNNNGTC	1 cut(s)	1027
DseDI	GACNNNNNNGTC	1 cut(s)	1027
EaeI	YGGCCR	1 cut(s)	765
Eam1104I	CTCTTC	3 cut(s)	26, 56, 138
EarI	CTCTTC	3 cut(s)	26, 56, 138
Eco130I	CCWWGG	2 cut(s)	768, 1605
Eco147I	AGGCCT	2 cut(s)	295, 584
Eco31I	GGTCTC	3 cut(s)	53, 1047, 1277
Eco47I	GGWCC	4 cut(s)	87, 448, 971, 1308
Eco57I	CTGAAG	3 cut(s)	7, 37, 722
Eco81I	CCTNAGG	1 cut(s)	1671
EcoNI	CCTNNNNNAGG	2 cut(s)	589, 1650
EcoO109I	RGGNCCY	1 cut(s)	87
EcoRII	CCWGG	6 cut(s)	241, 739, 857, 955, 1037, 1214
EcoT14I	CCWWGG	2 cut(s)	768, 1605
ErhI	CCWWGG	2 cut(s)	768, 1605
FalI	AAGNNNNNCTT	2 cut(s)	572, 604
FaqI	GGGAC	2 cut(s)	475, 1869
FauI	CCCGC	2 cut(s)	99, 1700
FbaI	TGATCA	1 cut(s)	1201
Fnu4HI	GCNGC	4 cut(s)	179, 357, 873, 1492
FokI	GGATG	5 cut(s)	547, 796, 824, 954, 1580
Fsp4HI	GCNGC	4 cut(s)	179, 357, 873, 1492
FspAI	RTGCGCAY	1 cut(s)	529
FspBI	CTAG	2 cut(s)	84, 839
FspI	TGCGCA	1 cut(s)	529
GlaI	GCGC	3 cut(s)	82, 247, 529
GluI	GCNGC	4 cut(s)	179, 357, 873, 1492
GsaI	CCCAGC	1 cut(s)	905
GsuI	CTGGAG	1 cut(s)	1139
HaeII	RGCGCY	1 cut(s)	249
HaeIII	GGCC	8 cut(s)	295, 319, 584, 767, 916, 1187, 1626, 1676
HgaI	GACGC	2 cut(s)	704, 1889
HhaI	GCGC	3 cut(s)	83, 248, 530
Hin1I	GRCGYC	1 cut(s)	1110
Hin6I	GCGC	3 cut(s)	81, 246, 528
HinP1I	GCGC	3 cut(s)	81, 246, 528
HincII	GTYRAC	2 cut(s)	256, 790
HindII	GTYRAC	2 cut(s)	256, 790
HinfI	GANTC	8 cut(s)	116, 257, 391, 697, 821, 1095, 1127, 1403
HphI	GGTGA	3 cut(s)	668, 1545, 1621
Hpy166II	GTNNAC	5 cut(s)	256, 345, 416, 790, 1311
Hpy188I	TCNGA	6 cut(s)	139, 694, 702, 1236, 1823, 1843
Hpy8I	GTNNAC	5 cut(s)	256, 345, 416, 790, 1311
Hpy99I	CGWCG	3 cut(s)	328, 1115, 1883
HpyAV	CCTTC	7 cut(s)	194, 331, 595, 1450, 1522, 1646, 1829
HpyCH4III	ACNGT	6 cut(s)	545, 609, 800, 971, 1860, 1934
HpyCH4IV	ACGT	2 cut(s)	1110, 1533
HpyF10VI	GCNNNNNNNGC	6 cut(s)	33, 428, 581, 712, 1781, 1790
HpyF3I	CTNAG	6 cut(s)	664, 922, 947, 1671, 1789, 1864
HpySE526I	ACGT	2 cut(s)	1110, 1533
Hsp92I	GRCGYC	1 cut(s)	1110
HspAI	GCGC	3 cut(s)	81, 246, 528
Ksp22I	TGATCA	1 cut(s)	1201
Kzo9I	GATC	8 cut(s)	276, 304, 952, 1201, 1330, 1486, 1642, 1838
LmnI	GCTCC	5 cut(s)	186, 887, 936, 1003, 1365
Lsp1109I	GCAGC	4 cut(s)	190, 368, 859, 1478
LweI	GCATC	3 cut(s)	525, 1279, 1558
MaeI	CTAG	2 cut(s)	84, 839
MaeII	ACGT	2 cut(s)	1110, 1533
MaeIII	GTNAC	3 cut(s)	594, 751, 1519
MalI	GATC	8 cut(s)	278, 306, 954, 1203, 1332, 1488, 1644, 1840
MbiI	CCGCTC	1 cut(s)	1695
MboI	GATC	8 cut(s)	276, 304, 952, 1201, 1330, 1486, 1642, 1838
MboII	GAAGA	7 cut(s)	13, 43, 155, 495, 671, 1239, 1725
MfeI	CAATTG	1 cut(s)	1299
MflI	RGATCY	1 cut(s)	1330
MlsI	TGGCCA	1 cut(s)	767
MluCI	AATT	8 cut(s)	383, 1085, 1299, 1316, 1462, 1638, 1744, 1884
MluNI	TGGCCA	1 cut(s)	767
MlyI	GAGTC	2 cut(s)	110, 251
MmeI	TCCRAC	1 cut(s)	83
Mox20I	TGGCCA	1 cut(s)	767
MroXI	GAANNNNTTC	1 cut(s)	341
MscI	TGGCCA	1 cut(s)	767
MseI	TTAA	7 cut(s)	513, 1088, 1191, 1391, 1536, 1589, 1680
MslI	CAYNNNNRTG	4 cut(s)	195, 350, 529, 554
Msp20I	TGGCCA	1 cut(s)	767
MspR9I	CCNGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
MunI	CAATTG	1 cut(s)	1299
Mva1269I	GAATGC	1 cut(s)	1183
MvaI	CCWGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
MvnI	CGCG	1 cut(s)	81
MwoI	GCNNNNNNNGC	6 cut(s)	33, 428, 581, 712, 1781, 1790
NdeII	GATC	8 cut(s)	276, 304, 952, 1201, 1330, 1486, 1642, 1838
NlaIV	GGNNCC	7 cut(s)	89, 450, 745, 889, 1380, 1603, 1828
NmeAIII	GCCGAG	1 cut(s)	1731
NmuCI	GTSAC	1 cut(s)	594
NsbI	TGCGCA	1 cut(s)	529
NspI	RCATGY	4 cut(s)	528, 796, 1265, 1502
NspV	TTCGAA	1 cut(s)	1526
OliI	CACNNNNGTG	1 cut(s)	350
PaeI	GCATGC	1 cut(s)	1502
PagI	TCATGA	2 cut(s)	399, 549
PaqCI	CACCTGC	1 cut(s)	766
PceI	AGGCCT	2 cut(s)	295, 584
PciI	ACATGT	1 cut(s)	792
PcsI	WCGNNNNNNNCGW	1 cut(s)	116
PctI	GAATGC	1 cut(s)	1183
PdmI	GAANNNNTTC	1 cut(s)	341
PfeI	GAWTC	6 cut(s)	391, 697, 821, 1095, 1127, 1403
PflMI	CCANNNNNTGG	1 cut(s)	102
PfoI	TCCNGGA	1 cut(s)	739
PkrI	GCNGC	4 cut(s)	180, 358, 874, 1493
PleI	GAGTC	2 cut(s)	110, 251
PpsI	GAGTC	2 cut(s)	110, 251
PpuMI	RGGWCCY	1 cut(s)	87
PscI	ACATGT	1 cut(s)	792
PshBI	ATTAAT	1 cut(s)	1088
Psp5II	RGGWCCY	1 cut(s)	87
Psp6I	CCWGG	6 cut(s)	241, 739, 857, 955, 1037, 1214
PspFI	CCCAGC	1 cut(s)	901
PspGI	CCWGG	6 cut(s)	241, 739, 857, 955, 1037, 1214
PspN4I	GGNNCC	7 cut(s)	89, 450, 745, 889, 1380, 1603, 1828
PspPI	GGNCC	6 cut(s)	87, 318, 448, 971, 1185, 1308
PspPPI	RGGWCCY	1 cut(s)	87
PstI	CTGCAG	1 cut(s)	1777
PsuI	RGATCY	1 cut(s)	1330
RsaI	GTAC	6 cut(s)	366, 415, 672, 797, 1862, 1902
RsaNI	GTAC	6 cut(s)	365, 414, 671, 796, 1861, 1901
RseI	CAYNNNNRTG	4 cut(s)	195, 350, 529, 554
SaqAI	TTAA	7 cut(s)	513, 1088, 1191, 1391, 1536, 1589, 1680
SatI	GCNGC	4 cut(s)	179, 357, 873, 1492
Sau3AI	GATC	8 cut(s)	276, 304, 952, 1201, 1330, 1486, 1642, 1838
Sau96I	GGNCC	6 cut(s)	87, 318, 448, 971, 1185, 1308
SchI	GAGTC	2 cut(s)	110, 251
ScrFI	CCNGG	6 cut(s)	243, 741, 859, 957, 1039, 1216
SfaNI	GCATC	3 cut(s)	525, 1279, 1558
SfcI	CTRYAG	2 cut(s)	1773, 1933
SfuI	TTCGAA	1 cut(s)	1526
SinI	GGWCC	4 cut(s)	87, 448, 971, 1308
SmiMI	CAYNNNNRTG	4 cut(s)	195, 350, 529, 554
SmlI	CTYRAG	3 cut(s)	589, 710, 1360
SmoI	CTYRAG	3 cut(s)	589, 710, 1360
SphI	GCATGC	1 cut(s)	1502
Sse9I	AATT	8 cut(s)	383, 1085, 1299, 1316, 1462, 1638, 1744, 1884
SseBI	AGGCCT	2 cut(s)	295, 584
SsiI	CCGC	2 cut(s)	92, 1693
SspMI	CTAG	2 cut(s)	84, 839
StuI	AGGCCT	2 cut(s)	295, 584
StyD4I	CCNGG	6 cut(s)	241, 739, 857, 955, 1037, 1214
StyI	CCWWGG	2 cut(s)	768, 1605
TaaI	ACNGT	6 cut(s)	545, 609, 800, 971, 1860, 1934
TaiI	ACGT	2 cut(s)	1113, 1536
TaqI	TCGA	4 cut(s)	279, 326, 1223, 1526
TaqII	GACCGA	2 cut(s)	35, 1341
TasI	AATT	8 cut(s)	383, 1085, 1299, 1316, 1462, 1638, 1744, 1884
TatI	WGTACW	2 cut(s)	364, 413
TfiI	GAWTC	6 cut(s)	391, 697, 821, 1095, 1127, 1403
Tru1I	TTAA	7 cut(s)	513, 1088, 1191, 1391, 1536, 1589, 1680
Tru9I	TTAA	7 cut(s)	513, 1088, 1191, 1391, 1536, 1589, 1680
TscAI	CASTG	2 cut(s)	1923, 1937
TseFI	GTSAC	1 cut(s)	594
TseI	GCWGC	4 cut(s)	178, 356, 872, 1491
Tsp45I	GTSAC	1 cut(s)	594
TspDTI	ATGAA	7 cut(s)	17, 383, 538, 813, 1098, 1146, 1878
TspGWI	ACGGA	3 cut(s)	1367, 1448, 1614
TspRI	CASTG	2 cut(s)	1923, 1937
Van91I	CCANNNNNTGG	1 cut(s)	102
VpaK11BI	GGWCC	4 cut(s)	87, 448, 971, 1308
VspI	ATTAAT	1 cut(s)	1088
XagI	CCTNNNNNAGG	2 cut(s)	589, 1650
XapI	RAATTY	1 cut(s)	383
XceI	RCATGY	4 cut(s)	528, 796, 1265, 1502
XcmI	CCANNNNNNNNNTGG	2 cut(s)	898, 1603
XmnI	GAANNNNTTC	1 cut(s)	341
XspI	CTAG	2 cut(s)	84, 839
ZraI	GACGTC	1 cut(s)	1111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.