Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0012236

Physical Location & Seq

Genomic Coordinates Forward (+)

49599135 .. 49603181

Gene Structure 4047 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG331000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1923 bp

ATGAAGAAAATCTTAGCCTTCAGTTTTCTCTGCTTGTTCCTGGGTTTTCACACCCAGAGGGTATCATCACAAAAACATGCTAGCACCCCACCCAGAGGTTGGAATTCCTATGACTCCTTTTGTTGGACTATTTCCGAGGAAGAGTTCTTGGAAAGTGCTGATATCATAGCTAAGCAGCTACTTCCACATGGATATGAGTATGCTGTTGTCGATTACCTGTGGTATAGGAGAAAGGTTCCAGGCGCTTACGTTGATTCTCTAGGATTTGATGTAATCGACCAATGGGGGAGGCCTATACCTGACCCAGGTAGGTGGCCTTCCTCCAAAGGTGGGAATGGGTTCACTAAAGTGGCATCAAAAGTACATCAGCTGGGTTTGAAGTTTGGAATTCATCTTATGAGAGGAATTAGTACTCAGGCAGTAAATGCGAATACCCCTATCTTGGATGTCACCAAGGGGACTGCTTATGAAGAGTCTGGAAGAACATGGACTGCAAAAGACATAGGGATCAATGAAAGGCGTTGCGCATGGATGCAAAATGGTTTCATGAGTGTGAATACTACATTGGGTGCTGGGAAGGCCTTCTTAAGATCACTTTATCGACAGTATGACGAGTGGGGTGTTGATTTTGTGAAAAATGATTGTGTGTTTGGTGAAGACATAGATGTAGGTGAAATAACCGTTGTGTCAGAGGTTCTAAAGCAACTTGACCATCCTGTGGTGTATTCTCTCTCTCCTGGAACCCAAGCAACACCTGCGCTGGCCAAAGATCTAACTGGTATAGTCAATATGTACCGTATAACAGGGGATGATTGGGACACATGGGACGATGTTGTAGCCCATTTTGATGTTACCAGGGATTTTGCTGCTGCGAATTTGATAGGAGCTAATGGCTTGCTGGGAAAGTCATGGCCTGACTTAGATATGCTCCCCCTGGGATGGCTTACCGATCCAGGTTCAAATGATGGTCCACACAGACAAACAAAACTCACTTTAGACGAGCAAAGAACTCAGATGACTTTGTGGTCTATTGCCAAGTCTCCTCTCATGTTTGGAGGGGATGTGAGAAAGCTTGATGATGCCACAACTAGTCTCATCACAAATCCTACCTTGCTGGAAATTAATTCGTTTAGCTCCAACAATAAGGAGTTTCCATTGATTTCAAGTACTTCGAGTAAAAGGGACGTCAAGAATGATGATCTGGTTAACACCTGGCAATCAAGAAGACAATTAAAAAGTTTAAAGACTTCAGATTCACATGTTCTTGGTCTCACTAGCTGTGCAGATGAGAAAGCAATTGGGTGGTCCGCTAAAGCTCTTGACCAAGATCTTGAACACATCTGCTGGAAAGAACACTCGAGAAAGAAATACCGGACACCTTTTTGCTTAAACAAGAGAGAATCTCTTTTGGCATTCGAAGACGACATAATGTACAAACAGCAATACCAGGGGAAGGTCAATTTGTTAGCTAGTGACAGGGAGAACATGTGCTTGCATGGTTCTCCAAAACGAAAGCTTACTTCGAAAGATCTAAAGAGAGGTTTCTTTTCACCTTGCAGATGGGATGCAAATCAGATGTGGGAGTTGAACCCCAATGGAACCTTGGCAAATAGCTATTCTGGTCTATGTGCAACAGTAAATTCTTTTAAAGCTAAAGCCAATTCTAAAGGAGCTCGTGCTTGGATAGCCACCGGAAGACAAGGAGAAATATATGTTGCTTTTTTCAACCTGAACTCAGCAAAAACTACTATAACTGCACAAAAATCAGACTTGGCAAAAGCACTTCCCGGCTCGAGAGTAACTGCAGGTTCCTGCAAAGCCGCAGAAGTATGGAGCGGACAAGATTTGGGGATTATAGACCAGACCATATCATTTGGTGTAGAAACTCATGGGGCTGCTCTATTTGTTCTACAGTGTAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005994 GO:0005995 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006672 GO:0006677 GO:0006687 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009987 GO:0015925 GO:0016042 GO:0016052 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019377 GO:0030149 GO:0034641 GO:0043603 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044262 GO:0044275 GO:0044424 GO:0044464 GO:0046352 GO:0046466 GO:0046477 GO:0046479 GO:0046514 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1903509

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Melibiase_2

Protein Analysis

640

Amino Acids

71.26

Weight (kDa)

7.88

Isoelectric Point (pI)

30.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	202 - 375	1.5e-11	Alpha galactosidase A
Melibiase_C	PF17801	558 - 637	2.5e-08	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26380 AT3G26380
fragaria_vesca	FvH4_4g25930
malus_domestica	MD13G1098300.v1.1 MD13G1098400.v1.1 MD16G1099400.v1.1
prunus_persica	Prupe.1G247000_v2.0.a1
pyrus_communis	pycom13g08600 pycom16g08460
rosa_chinensis	RchiOBHm_Chr4g0433401
rosa_laevigata	RLG00000006756
rosa_multiflora	Rmu_sc0000759.1_g000006
rosa_roxburghii	Rroxscaffold_5G00374950
rosa_rugosa	Rorug04G0268500
rosa_samantha	Rh4AG323300 Rh4BG331000 Rh4CG346300 Rh4DG327400
rosa_wichuraiana	Rw4G028000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	763
AasI	GACNNNNNNGTC	1 cut(s)	1024
AatII	GACGTC	1 cut(s)	1188
Acc16I	TGCGCA	1 cut(s)	526
Acc36I	ACCTGC	2 cut(s)	763, 1796
AccB7I	CCANNNNNTGG	2 cut(s)	99, 718
AccBSI	CCGCTC	1 cut(s)	1836
AciI	CCGC	3 cut(s)	1308, 1821, 1836
AclWI	GGATC	2 cut(s)	515, 944
AcoI	YGGCCR	1 cut(s)	762
AcsI	RAATTY	4 cut(s)	103, 387, 874, 1639
AcuI	CTGAAG	2 cut(s)	4, 1233
AcyI	GRCGYC	1 cut(s)	1185
AfaI	GTAC	5 cut(s)	363, 412, 794, 1168, 1433
AfiI	CCNNNNNNNGG	9 cut(s)	95, 99, 123, 305, 330, 442, 718, 939, 1453
AflII	CTTAAG	1 cut(s)	586
AflIII	ACRYGT	2 cut(s)	1258, 1485
AgsI	TTSAA	6 cut(s)	379, 960, 1164, 1334, 1588, 1726
AhlI	ACTAGT	1 cut(s)	1088
AjnI	CCWGG	9 cut(s)	39, 238, 304, 736, 854, 933, 952, 1211, 1446
AjuI	GAANNNNNNNTTGG	2 cut(s)	548, 580
AleI	CACNNNNGTG	1 cut(s)	347
Alw21I	GWGCWC	1 cut(s)	1675
Alw26I	GTCTC	3 cut(s)	1044, 1097, 1274
AlwI	GGATC	2 cut(s)	515, 944
Ama87I	CYCGRG	2 cut(s)	1357, 1792
AoxI	GGCC	5 cut(s)	290, 314, 579, 762, 911
ApeKI	GCWGC	4 cut(s)	175, 866, 869, 1895
ApoI	RAATTY	4 cut(s)	103, 387, 874, 1639
AseI	ATTAAT	1 cut(s)	1122
Asp700I	GAANNNNTTC	2 cut(s)	338, 581
AspLEI	GCGC	3 cut(s)	245, 527, 760
AspS9I	GGNCC	2 cut(s)	968, 1305
AsuC2I	CCSGG	1 cut(s)	1788
AsuHPI	GGTGA	4 cut(s)	442, 665, 683, 1542
AsuII	TTCGAA	2 cut(s)	1416, 1523
AsuNHI	GCTAGC	1 cut(s)	80
AvaI	CYCGRG	2 cut(s)	1357, 1792
AvaII	GGWCC	2 cut(s)	968, 1305
BalI	TGGCCA	1 cut(s)	764
BanII	GRGCYC	1 cut(s)	1675
BarI	GAAGNNNNNNTAC	2 cut(s)	301, 333
BauI	CACGAG	1 cut(s)	1674
BbsI	GAAGAC	4 cut(s)	663, 1231, 1425, 1702
Bbv12I	GWGCWC	1 cut(s)	1675
BbvI	GCAGC	4 cut(s)	187, 853, 856, 1882
BccI	CCATC	4 cut(s)	720, 933, 959, 1554
BciT130I	CCWGG	9 cut(s)	41, 240, 306, 738, 856, 935, 954, 1213, 1448
BcnI	CCSGG	1 cut(s)	1788
BcoDI	GTCTC	3 cut(s)	1044, 1097, 1274
BcuI	ACTAGT	1 cut(s)	1088
BfaI	CTAG	5 cut(s)	81, 260, 1089, 1275, 1470
BfmI	CTRYAG	2 cut(s)	1803, 1910
BfoI	RGCGCY	1 cut(s)	246
BfrI	CTTAAG	1 cut(s)	586
BfuAI	ACCTGC	2 cut(s)	763, 1796
BglII	AGATCT	3 cut(s)	769, 1327, 1528
BisI	GCNGC	5 cut(s)	176, 867, 870, 1821, 1896
BlpI	GCTNAGC	1 cut(s)	171
BlsI	GCNGC	5 cut(s)	177, 868, 871, 1822, 1897
BmcAI	AGTACT	2 cut(s)	412, 1168
Bme18I	GGWCC	2 cut(s)	968, 1305
BmeT110I	CYCGRG	2 cut(s)	1357, 1792
BmgT120I	GGNCC	2 cut(s)	968, 1305
BmiI	GGNNCC	4 cut(s)	237, 742, 1600, 1810
BmsI	GCATC	4 cut(s)	362, 522, 1069, 1555
BmtI	GCTAGC	1 cut(s)	84
BpiI	GAAGAC	4 cut(s)	663, 1231, 1425, 1702
BplI	GAGNNNNNCTC	2 cut(s)	1387, 1419
Bpu1102I	GCTNAGC	1 cut(s)	171
Bpu14I	TTCGAA	2 cut(s)	1416, 1523
BpuMI	CCSGG	1 cut(s)	1788
BsaBI	GATNNNNATC	1 cut(s)	1569
BsaHI	GRCGYC	1 cut(s)	1185
BsaI	GGTCTC	1 cut(s)	1274
BsaJI	CCNNGG	9 cut(s)	40, 135, 304, 453, 855, 933, 934, 1447, 1602
BsaWI	WCCGGW	2 cut(s)	1371, 1691
BsaXI	ACNNNNNCTCC	4 cut(s)	1047, 1077, 1472, 1502
Bsc4I	CCNNNNNNNGG	9 cut(s)	95, 99, 123, 305, 330, 442, 718, 939, 1453
Bse1I	ACTGG	1 cut(s)	781
Bse8I	GATNNNNATC	1 cut(s)	1569
BseBI	CCWGG	9 cut(s)	41, 240, 306, 738, 856, 935, 954, 1213, 1448
BseDI	CCNNGG	9 cut(s)	40, 135, 304, 453, 855, 933, 934, 1447, 1602
BseGI	GGATG	7 cut(s)	451, 537, 712, 814, 944, 1066, 1570
BseJI	GATNNNNATC	1 cut(s)	1569
BseLI	CCNNNNNNNGG	9 cut(s)	95, 99, 123, 305, 330, 442, 718, 939, 1453
BseMII	CTCAG	3 cut(s)	428, 1025, 1749
BseNI	ACTGG	1 cut(s)	781
BseRI	GAGGAG	1 cut(s)	1032
BseXI	GCAGC	4 cut(s)	187, 853, 856, 1882
BseYI	CCCAGC	3 cut(s)	370, 572, 898
BsgI	GTGCAG	2 cut(s)	1302, 1740
BshFI	GGCC	5 cut(s)	292, 316, 581, 764, 913
BsiHKAI	GWGCWC	1 cut(s)	1675
BsiHKCI	CYCGRG	2 cut(s)	1357, 1792
BsiSI	CCGG	3 cut(s)	1372, 1692, 1788
BslFI	GGGAC	4 cut(s)	472, 830, 839, 1196
BslI	CCNNNNNNNGG	9 cut(s)	95, 99, 123, 305, 330, 442, 718, 939, 1453
BsmAI	GTCTC	3 cut(s)	1044, 1097, 1274
BsmFI	GGGAC	4 cut(s)	472, 830, 839, 1196
BsmI	GAATGC	1 cut(s)	1412
BsnI	GGCC	5 cut(s)	292, 316, 581, 764, 913
Bso31I	GGTCTC	1 cut(s)	1274
BsoBI	CYCGRG	2 cut(s)	1357, 1792
Bsp119I	TTCGAA	2 cut(s)	1416, 1523
Bsp1286I	GDGCHC	1 cut(s)	1675
Bsp1407I	TGTACA	1 cut(s)	1431
Bsp143I	GATC	7 cut(s)	507, 590, 769, 949, 1198, 1327, 1528
Bsp1720I	GCTNAGC	1 cut(s)	171
BspACI	CCGC	3 cut(s)	1308, 1821, 1836
BspANI	GGCC	5 cut(s)	292, 316, 581, 764, 913
BspCNI	CTCAG	3 cut(s)	427, 1024, 1748
BspHI	TCATGA	1 cut(s)	546
BspLI	GGNNCC	4 cut(s)	237, 742, 1600, 1810
BspMAI	CTGCAG	1 cut(s)	1807
BspMI	ACCTGC	2 cut(s)	763, 1796
BspOI	GCTAGC	1 cut(s)	84
BspPI	GGATC	2 cut(s)	515, 944
BspT104I	TTCGAA	2 cut(s)	1416, 1523
BspTI	CTTAAG	1 cut(s)	586
BspTNI	GGTCTC	1 cut(s)	1274
BsrBI	CCGCTC	1 cut(s)	1836
BsrGI	TGTACA	1 cut(s)	1431
BsrI	ACTGG	1 cut(s)	781
BssECI	CCNNGG	9 cut(s)	40, 135, 304, 453, 855, 933, 934, 1447, 1602
BssMI	GATC	7 cut(s)	507, 590, 769, 949, 1198, 1327, 1528
BssNI	GRCGYC	1 cut(s)	1185
BssSI	CACGAG	1 cut(s)	1674
BssT1I	CCWWGG	2 cut(s)	453, 1602
Bst2BI	CACGAG	1 cut(s)	1674
Bst2UI	CCWGG	9 cut(s)	41, 240, 306, 738, 856, 935, 954, 1213, 1448
Bst4CI	ACNGT	5 cut(s)	606, 682, 797, 1636, 1914
Bst6I	CTCTTC	2 cut(s)	135, 465
BstACI	GRCGYC	1 cut(s)	1185
BstAFI	CTTAAG	1 cut(s)	586
BstAPI	GCANNNNNTGC	2 cut(s)	425, 755
BstAUI	TGTACA	1 cut(s)	1431
BstBI	TTCGAA	2 cut(s)	1416, 1523
BstC8I	GCNNGC	4 cut(s)	82, 762, 896, 1493
BstDEI	CTNAG	6 cut(s)	13, 171, 414, 919, 1011, 1735
BstENI	CCTNNNNNAGG	1 cut(s)	303
BstF5I	GGATG	7 cut(s)	451, 537, 712, 814, 944, 1066, 1570
BstH2I	RGCGCY	1 cut(s)	246
BstHHI	GCGC	3 cut(s)	245, 527, 760
BstKTI	GATC	7 cut(s)	510, 593, 772, 952, 1201, 1330, 1531
BstMAI	GTCTC	3 cut(s)	1044, 1097, 1274
BstMBI	GATC	7 cut(s)	507, 590, 769, 949, 1198, 1327, 1528
BstMWI	GCNNNNNNNGC	4 cut(s)	425, 578, 755, 1685
BstNI	CCWGG	9 cut(s)	41, 240, 306, 738, 856, 935, 954, 1213, 1448
BstNSI	RCATGY	3 cut(s)	80, 1262, 1489
BstSFI	CTRYAG	2 cut(s)	1803, 1910
BstV1I	GCAGC	4 cut(s)	187, 853, 856, 1882
BstV2I	GAAGAC	4 cut(s)	663, 1231, 1425, 1702
BstX2I	RGATCY	3 cut(s)	769, 1327, 1528
BstXI	CCANNNNNNTGG	1 cut(s)	312
BstYI	RGATCY	3 cut(s)	769, 1327, 1528
BsuRI	GGCC	5 cut(s)	292, 316, 581, 764, 913
BtsCI	GGATG	7 cut(s)	451, 537, 712, 814, 944, 1066, 1570
BtsIMutI	CAGTG	1 cut(s)	1919
BveI	ACCTGC	2 cut(s)	763, 1796
Cac8I	GCNNGC	4 cut(s)	82, 762, 896, 1493
CciI	TCATGA	1 cut(s)	546
CfoI	GCGC	3 cut(s)	245, 527, 760
Cfr13I	GGNCC	2 cut(s)	968, 1305
Csp6I	GTAC	5 cut(s)	362, 411, 793, 1167, 1432
CviQI	GTAC	5 cut(s)	362, 411, 793, 1167, 1432
DdeI	CTNAG	6 cut(s)	13, 171, 414, 919, 1011, 1735
DpnI	GATC	7 cut(s)	509, 592, 771, 951, 1200, 1329, 1530
DpnII	GATC	7 cut(s)	507, 590, 769, 949, 1198, 1327, 1528
DraI	TTTAAA	2 cut(s)	1242, 1648
DrdI	GACNNNNNNGTC	1 cut(s)	1024
DseDI	GACNNNNNNGTC	1 cut(s)	1024
EaeI	YGGCCR	1 cut(s)	762
Eam1104I	CTCTTC	2 cut(s)	135, 465
EarI	CTCTTC	2 cut(s)	135, 465
Ecl136II	GAGCTC	1 cut(s)	1673
Eco130I	CCWWGG	2 cut(s)	453, 1602
Eco147I	AGGCCT	2 cut(s)	292, 581
Eco24I	GRGCYC	1 cut(s)	1675
Eco31I	GGTCTC	1 cut(s)	1274
Eco32I	GATATC	1 cut(s)	163
Eco47I	GGWCC	2 cut(s)	968, 1305
Eco53kI	GAGCTC	1 cut(s)	1673
Eco57I	CTGAAG	2 cut(s)	4, 1233
Eco88I	CYCGRG	2 cut(s)	1357, 1792
EcoICRI	GAGCTC	1 cut(s)	1673
EcoNI	CCTNNNNNAGG	1 cut(s)	303
EcoRI	GAATTC	2 cut(s)	103, 387
EcoRII	CCWGG	9 cut(s)	39, 238, 304, 736, 854, 933, 952, 1211, 1446
EcoRV	GATATC	1 cut(s)	163
EcoT14I	CCWWGG	2 cut(s)	453, 1602
EcoT38I	GRGCYC	1 cut(s)	1675
ErhI	CCWWGG	2 cut(s)	453, 1602
FalI	AAGNNNNNCTT	3 cut(s)	28, 569, 601
FaqI	GGGAC	4 cut(s)	472, 830, 839, 1196
Fnu4HI	GCNGC	5 cut(s)	176, 867, 870, 1821, 1896
FokI	GGATG	7 cut(s)	458, 544, 699, 821, 951, 1073, 1577
FriOI	GRGCYC	1 cut(s)	1675
Fsp4HI	GCNGC	5 cut(s)	176, 867, 870, 1821, 1896
FspBI	CTAG	5 cut(s)	81, 260, 1089, 1275, 1470
FspI	TGCGCA	1 cut(s)	526
GlaI	GCGC	3 cut(s)	244, 526, 759
GluI	GCNGC	5 cut(s)	176, 867, 870, 1821, 1896
GsaI	CCCAGC	3 cut(s)	374, 576, 902
HaeII	RGCGCY	1 cut(s)	246
HaeIII	GGCC	5 cut(s)	292, 316, 581, 764, 913
HapII	CCGG	3 cut(s)	1372, 1692, 1788
HhaI	GCGC	3 cut(s)	245, 527, 760
Hin1I	GRCGYC	1 cut(s)	1185
Hin6I	GCGC	3 cut(s)	243, 525, 758
HinP1I	GCGC	3 cut(s)	243, 525, 758
HincII	GTYRAC	1 cut(s)	1207
HindII	GTYRAC	1 cut(s)	1207
HindIII	AAGCTT	2 cut(s)	1070, 1514
HinfI	GANTC	5 cut(s)	113, 254, 473, 1253, 1400
HpaI	GTTAAC	1 cut(s)	1207
HpaII	CCGG	3 cut(s)	1372, 1692, 1788
HphI	GGTGA	4 cut(s)	442, 665, 683, 1542
Hpy166II	GTNNAC	3 cut(s)	342, 971, 1207
Hpy188I	TCNGA	6 cut(s)	136, 691, 1014, 1252, 1575, 1768
Hpy188III	TCNNGA	8 cut(s)	477, 547, 1189, 1221, 1319, 1331, 1359, 1794
Hpy8I	GTNNAC	3 cut(s)	342, 971, 1207
HpyAV	CCTTC	5 cut(s)	28, 327, 571, 592, 1447
HpyCH4III	ACNGT	5 cut(s)	606, 682, 797, 1636, 1914
HpyCH4IV	ACGT	2 cut(s)	249, 1185
HpyF10VI	GCNNNNNNNGC	4 cut(s)	425, 578, 755, 1685
HpyF3I	CTNAG	6 cut(s)	13, 171, 414, 919, 1011, 1735
HpySE526I	ACGT	2 cut(s)	249, 1185
Hsp92I	GRCGYC	1 cut(s)	1185
HspAI	GCGC	3 cut(s)	243, 525, 758
KspAI	GTTAAC	1 cut(s)	1207
Kzo9I	GATC	7 cut(s)	507, 590, 769, 949, 1198, 1327, 1528
LmnI	GCTCC	5 cut(s)	884, 933, 1139, 1670, 1833
Lsp1109I	GCAGC	4 cut(s)	187, 853, 856, 1882
LweI	GCATC	4 cut(s)	362, 522, 1069, 1555
MaeI	CTAG	5 cut(s)	81, 260, 1089, 1275, 1470
MaeII	ACGT	2 cut(s)	249, 1185
MaeIII	GTNAC	4 cut(s)	448, 850, 1472, 1798
MalI	GATC	7 cut(s)	509, 592, 771, 951, 1200, 1329, 1530
MbiI	CCGCTC	1 cut(s)	1836
MboI	GATC	7 cut(s)	507, 590, 769, 949, 1198, 1327, 1528
MboII	GAAGA	8 cut(s)	16, 152, 482, 492, 668, 1236, 1430, 1707
MfeI	CAATTG	1 cut(s)	1296
MflI	RGATCY	3 cut(s)	769, 1327, 1528
MhlI	GDGCHC	1 cut(s)	1675
MlsI	TGGCCA	1 cut(s)	764
MluNI	TGGCCA	1 cut(s)	764
MlyI	GAGTC	2 cut(s)	107, 482
MmeI	TCCRAC	3 cut(s)	80, 104, 1161
Mox20I	TGGCCA	1 cut(s)	764
MroXI	GAANNNNTTC	2 cut(s)	338, 581
MscI	TGGCCA	1 cut(s)	764
MseI	TTAA	7 cut(s)	587, 1122, 1206, 1232, 1241, 1388, 1647
MslI	CAYNNNNRTG	4 cut(s)	192, 347, 551, 846
Msp20I	TGGCCA	1 cut(s)	764
MspA1I	CMGCKG	1 cut(s)	370
MspCI	CTTAAG	1 cut(s)	586
MspI	CCGG	3 cut(s)	1372, 1692, 1788
MunI	CAATTG	1 cut(s)	1296
Mva1269I	GAATGC	1 cut(s)	1412
MvaI	CCWGG	9 cut(s)	41, 240, 306, 738, 856, 935, 954, 1213, 1448
MwoI	GCNNNNNNNGC	4 cut(s)	425, 578, 755, 1685
NciI	CCSGG	1 cut(s)	1788
NdeII	GATC	7 cut(s)	507, 590, 769, 949, 1198, 1327, 1528
NheI	GCTAGC	1 cut(s)	80
NlaIV	GGNNCC	4 cut(s)	237, 742, 1600, 1810
NmuCI	GTSAC	2 cut(s)	448, 1472
NsbI	TGCGCA	1 cut(s)	526
NspI	RCATGY	3 cut(s)	80, 1262, 1489
NspV	TTCGAA	2 cut(s)	1416, 1523
OliI	CACNNNNGTG	1 cut(s)	347
PaeR7I	CTCGAG	2 cut(s)	1357, 1792
PagI	TCATGA	1 cut(s)	546
PaqCI	CACCTGC	1 cut(s)	763
PasI	CCCWGGG	1 cut(s)	934
PceI	AGGCCT	2 cut(s)	292, 581
PciI	ACATGT	2 cut(s)	1258, 1485
PctI	GAATGC	1 cut(s)	1412
PdmI	GAANNNNTTC	2 cut(s)	338, 581
PfeI	GAWTC	3 cut(s)	254, 1253, 1400
PflMI	CCANNNNNTGG	2 cut(s)	99, 718
PfoI	TCCNGGA	1 cut(s)	736
PkrI	GCNGC	5 cut(s)	177, 868, 871, 1822, 1897
PleI	GAGTC	2 cut(s)	107, 481
PpsI	GAGTC	2 cut(s)	107, 481
PscI	ACATGT	2 cut(s)	1258, 1485
PshBI	ATTAAT	1 cut(s)	1122
Psp124BI	GAGCTC	1 cut(s)	1675
Psp6I	CCWGG	9 cut(s)	39, 238, 304, 736, 854, 933, 952, 1211, 1446
PspFI	CCCAGC	3 cut(s)	370, 572, 898
PspGI	CCWGG	9 cut(s)	39, 238, 304, 736, 854, 933, 952, 1211, 1446
PspN4I	GGNNCC	4 cut(s)	237, 742, 1600, 1810
PspPI	GGNCC	2 cut(s)	968, 1305
PstI	CTGCAG	1 cut(s)	1807
PsuI	RGATCY	3 cut(s)	769, 1327, 1528
PvuII	CAGCTG	1 cut(s)	370
RsaI	GTAC	5 cut(s)	363, 412, 794, 1168, 1433
RsaNI	GTAC	5 cut(s)	362, 411, 793, 1167, 1432
RseI	CAYNNNNRTG	4 cut(s)	192, 347, 551, 846
SacI	GAGCTC	1 cut(s)	1675
SaqAI	TTAA	7 cut(s)	587, 1122, 1206, 1232, 1241, 1388, 1647
SatI	GCNGC	5 cut(s)	176, 867, 870, 1821, 1896
Sau3AI	GATC	7 cut(s)	507, 590, 769, 949, 1198, 1327, 1528
Sau96I	GGNCC	2 cut(s)	968, 1305
ScaI	AGTACT	2 cut(s)	412, 1168
SchI	GAGTC	2 cut(s)	107, 482
SduI	GDGCHC	1 cut(s)	1675
SfaNI	GCATC	4 cut(s)	362, 522, 1069, 1555
SfcI	CTRYAG	2 cut(s)	1803, 1910
Sfr274I	CTCGAG	2 cut(s)	1357, 1792
SfuI	TTCGAA	2 cut(s)	1416, 1523
SinI	GGWCC	2 cut(s)	968, 1305
SlaI	CTCGAG	2 cut(s)	1357, 1792
SmiMI	CAYNNNNRTG	4 cut(s)	192, 347, 551, 846
SmlI	CTYRAG	3 cut(s)	586, 1357, 1792
SmoI	CTYRAG	3 cut(s)	586, 1357, 1792
SpeI	ACTAGT	1 cut(s)	1088
SseBI	AGGCCT	2 cut(s)	292, 581
SsiI	CCGC	3 cut(s)	1308, 1821, 1836
SspMI	CTAG	5 cut(s)	81, 260, 1089, 1275, 1470
SstI	GAGCTC	1 cut(s)	1675
StuI	AGGCCT	2 cut(s)	292, 581
StyI	CCWWGG	2 cut(s)	453, 1602
TaaI	ACNGT	5 cut(s)	606, 682, 797, 1636, 1914
TaiI	ACGT	2 cut(s)	252, 1188
TaqI	TCGA	8 cut(s)	210, 276, 601, 1172, 1358, 1416, 1523, 1793
TatI	WGTACW	4 cut(s)	361, 410, 1166, 1431
TauI	GCSGC	1 cut(s)	1823
TfiI	GAWTC	3 cut(s)	254, 1253, 1400
Tru1I	TTAA	7 cut(s)	587, 1122, 1206, 1232, 1241, 1388, 1647
Tru9I	TTAA	7 cut(s)	587, 1122, 1206, 1232, 1241, 1388, 1647
TscAI	CASTG	1 cut(s)	1919
TseFI	GTSAC	2 cut(s)	448, 1472
TseI	GCWGC	4 cut(s)	175, 866, 869, 1895
Tsp45I	GTSAC	2 cut(s)	448, 1472
TspDTI	ATGAA	5 cut(s)	17, 380, 483, 528, 535
TspRI	CASTG	1 cut(s)	1919
Van91I	CCANNNNNTGG	2 cut(s)	99, 718
Vha464I	CTTAAG	1 cut(s)	586
VpaK11BI	GGWCC	2 cut(s)	968, 1305
VspI	ATTAAT	1 cut(s)	1122
XagI	CCTNNNNNAGG	1 cut(s)	303
XapI	RAATTY	4 cut(s)	103, 387, 874, 1639
XceI	RCATGY	3 cut(s)	80, 1262, 1489
XcmI	CCANNNNNNNNNTGG	2 cut(s)	96, 1600
XhoI	CTCGAG	2 cut(s)	1357, 1792
XmnI	GAANNNNTTC	2 cut(s)	338, 581
XspI	CTAG	5 cut(s)	81, 260, 1089, 1275, 1470
ZraI	GACGTC	1 cut(s)	1186
ZrmI	AGTACT	2 cut(s)	412, 1168

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.