Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012236

Physical Location & Seq

Genomic Coordinates Forward (+)

6974980 .. 6979251

Gene Structure 4272 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1098400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGAACACCTCCTTGTTTCTTTTCAGCTCTCTCTGTTTCTTCCTCGGTCTTCTCGGCCAGAGGGTTTCATCACAAGAACTTGCTAGCACCCCGCCGAGGGGTTGGAACTCGTATGACTCGTTTATCTGGGTTATTACGGAGGATGAGTTCCTGGAAAGTGCCCAAATTATGAAAGAGAAGCTCCTTCCTAAGGGATATGAGTATGCTGTGGTGGATTACCTGTGGTACAGGAAGAAAGTTCCTGGCGCTGATGTCAAATCTTATGGATTTGATGTAATCGATGAATGGGGGAGGCCGGTGCCTGATCCAGATAGGTGGCCTTCGTCGAAAGATGGGACAGGGTTCACTGAAGTGGCAGCATTTGTACATAGCTTCGGTCTGAAATTCGGGATCCATGTCATGAGAGGAATAAGTACACAGGCATTTGATGCGAATACCAAGATCTTGGACGTCAGAACGGGGTCTCCTTATGAAGAGGGTGGAAGACAATGGACTGCAAAAGATATAGGGATCCCGGACAGGAGGTGCGCATGGATGGACAAGGGATTCATGAGTGTGAATGCCGAGTTGGATGCCGGAAAGGCCTTCTTGCGGTCACTCTACTACCAGTATGCTGACTGGGGTGTTGATTTTGTAAAAAATGACTGCGTGTTTGGTGATGACTTAGATGTACCTGAAATAACCTATATTTCAGAGTATCTGCAGCAACTGCATGCACTTGGGCAACATCCTATATTGTATTCTCTATCTCCCGGAACCAGTGCAACGCCTGATATGGCCAAGCCTATAAATGGGATGGTCAACATGTACCGTATAACCGGGGATGATTGGGATTCATGGGGAGATATTCTAGCCCATTTTGATGTAGCCAGGGATTTTGCTGCTGCTAATATGATAGGAGCCAAAGGCATGCTGGGGAAGTCATGGCCTGACTTAGATATGCTACCGTTGGGATGGCTTAGTGCACCAGAAACAAATGACGGTCCACACAGATGGTCGAATCTCAATTTAGATGAGCAAAGAACACAGATGACTTTGTGGTCTATGGCCAAGTCTCCTATCATGTTTGGAGGAGATTTGAGAAAACTTACAGATGAATTAATGAGAATGCTCACAGATGATGCCTTGCTGGACATAAATTCTTTTAGCTCAAATAATAGGGAGTTTACACTCTTTACAAGTAATGTGAGCACAGCCGTTAAGCAAGATGATCGAGTTCGCACCAAGCAATCAAGAAGACATCTGACAAACTCAAAAACTTCAAATACACATTCTCTCGGCCTCACTACCTGTGCAGATGCGAAAGCAATTGGTTGGTCTGCTCAAGCTCTTGACCGAGATCTTGAACACATCTGCTGGAGAGATAACTCAAGGAGCAAACACCGGGCACCTTTTTGCTTAAACAAAAGAGATTCTCTCTTGGCAATAGATGAAGACATAACGGACAAACAGCGATATCAGGGGAAGGTCAATTTATTAGCAAGTGATAGAAAGGACCTTTGCTTGCATGCTTCTCCTACACAAAAGGTTACTTCAAAGGACGTTAAGAGAGGTTTCTTTTCACCTTGTAGATGGGATGCGAACCAGATGTGGGAGTTGAACCCCAATGGAACCTTGGTGAATAGTTATTCCGGCCTCTGCGCAAGAATTGATTCTTTTGAAGATAACAAAGCCGTCACTGATGTTGCTCGCTCTTGGATTGCAACTGGAACACCAGATGAAGTATACGTAGCGTTTTTCAATCTGGTTGGCGACTGGGCTACTATAACCGCGCAAAAAGCAGACATAGCCAAAGAACTTCCAATGACGAAATTGGCCAGCGGTTCGTGCAGGGGAATAGAAGAATGGACCGGACAAGACTTGGGGATTGTAGGCGACACAATTTCAGTTTCGGTTCCGTCGCACGGGGCTGCTCTGATTATCCTGACCTGCAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005994 GO:0005995 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006672 GO:0006677 GO:0006687 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009987 GO:0015925 GO:0016042 GO:0016052 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019377 GO:0030149 GO:0034641 GO:0043603 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044262 GO:0044275 GO:0044424 GO:0044464 GO:0046352 GO:0046466 GO:0046477 GO:0046479 GO:0046514 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1903509

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Melibiase_2

Protein Analysis

646

Amino Acids

72.21

Weight (kDa)

5.31

Isoelectric Point (pI)

33.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	204 - 380	4.4e-10	Alpha galactosidase A

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26380 AT3G26380
fragaria_vesca	FvH4_4g25930
malus_domestica	MD13G1098300.v1.1 MD13G1098400.v1.1 MD16G1099400.v1.1
prunus_persica	Prupe.1G247000_v2.0.a1
pyrus_communis	pycom13g08600 pycom16g08460
rosa_chinensis	RchiOBHm_Chr4g0433401
rosa_laevigata	RLG00000006756
rosa_multiflora	Rmu_sc0000759.1_g000006
rosa_roxburghii	Rroxscaffold_5G00374950
rosa_rugosa	Rorug04G0268500
rosa_samantha	Rh4AG323300 Rh4BG331000 Rh4CG346300 Rh4DG327400
rosa_wichuraiana	Rw4G028000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1039
AatII	GACGTC	1 cut(s)	453
Acc16I	TGCGCA	2 cut(s)	529, 1642
AccB1I	GGYRCC	2 cut(s)	298, 1387
AccI	GTMKAC	1 cut(s)	1725
AccII	CGCG	1 cut(s)	1772
AciI	CCGC	4 cut(s)	92, 592, 1770, 1821
AclWI	GGATC	5 cut(s)	299, 385, 398, 505, 518
AcoI	YGGCCR	4 cut(s)	55, 777, 1047, 1815
AcsI	RAATTY	2 cut(s)	383, 1138
AcuI	CTGAAG	1 cut(s)	369
AcyI	GRCGYC	1 cut(s)	450
AfaI	GTAC	5 cut(s)	227, 366, 415, 672, 809
AfiI	CCNNNNNNNGG	8 cut(s)	96, 97, 98, 190, 591, 791, 1591, 1904
AflIII	ACRYGT	1 cut(s)	804
AgsI	TTSAA	6 cut(s)	1263, 1346, 1536, 1600, 1661, 1741
AjnI	CCWGG	3 cut(s)	150, 241, 869
AjuI	GAANNNNNNNTTGG	2 cut(s)	551, 583
AleI	CACNNNNGTG	1 cut(s)	350
AloI	GAACNNNNNNTCC	4 cut(s)	131, 163, 448, 480
AluBI	AGCT	5 cut(s)	27, 181, 372, 1149, 1328
AluI	AGCT	5 cut(s)	27, 181, 372, 1149, 1328
Alw21I	GWGCWC	2 cut(s)	967, 1193
Alw26I	GTCTC	2 cut(s)	468, 1059
Alw44I	GTGCAC	1 cut(s)	963
AlwI	GGATC	5 cut(s)	299, 385, 398, 505, 518
AlwNI	CAGNNNCTG	1 cut(s)	709
ApaLI	GTGCAC	1 cut(s)	963
ApeKI	GCWGC	5 cut(s)	356, 703, 881, 884, 1910
ApoI	RAATTY	2 cut(s)	383, 1138
ArsI	GACNNNNNNTTYG	2 cut(s)	635, 667
AseI	ATTAAT	1 cut(s)	1100
Asp700I	GAANNNNTTC	1 cut(s)	1651
AspLEI	GCGC	4 cut(s)	248, 530, 1643, 1774
AspS9I	GGNCC	3 cut(s)	983, 1495, 1848
AsuC2I	CCSGG	4 cut(s)	515, 753, 820, 1385
AsuHPI	GGTGA	3 cut(s)	668, 1554, 1630
AsuNHI	GCTAGC	1 cut(s)	83
AvaII	GGWCC	3 cut(s)	983, 1495, 1848
AxyI	CCTNAGG	1 cut(s)	189
BaeGI	GKGCMC	3 cut(s)	163, 967, 1390
BalI	TGGCCA	3 cut(s)	779, 1049, 1817
BamHI	GGATCC	2 cut(s)	390, 510
BanI	GGYRCC	2 cut(s)	298, 1387
BbsI	GAAGAC	4 cut(s)	41, 490, 1243, 1440
Bbv12I	GWGCWC	2 cut(s)	967, 1193
BbvI	GCAGC	5 cut(s)	368, 715, 868, 871, 1897
BccI	CCATC	6 cut(s)	326, 529, 790, 948, 987, 1566
BceAI	ACGGC	2 cut(s)	1181, 1658
BcgI	CGANNNNNNTGC	4 cut(s)	554, 588, 1193, 1227
BciT130I	CCWGG	3 cut(s)	152, 243, 871
BcnI	CCSGG	4 cut(s)	515, 753, 820, 1385
BcoDI	GTCTC	2 cut(s)	468, 1059
BfaI	CTAG	2 cut(s)	84, 851
BfmI	CTRYAG	2 cut(s)	701, 1930
BfoI	RGCGCY	1 cut(s)	249
BglI	GCCNNNNNGGC	1 cut(s)	581
BglII	AGATCT	2 cut(s)	441, 1339
BisI	GCNGC	5 cut(s)	357, 704, 882, 885, 1911
BlsI	GCNGC	5 cut(s)	358, 705, 883, 886, 1912
Bme1390I	CCNGG	7 cut(s)	152, 243, 515, 753, 820, 871, 1385
Bme18I	GGWCC	3 cut(s)	983, 1495, 1848
BmgT120I	GGNCC	3 cut(s)	983, 1495, 1848
BmiI	GGNNCC	8 cut(s)	300, 392, 512, 757, 901, 1389, 1612, 1896
BmrFI	CCNGG	7 cut(s)	152, 243, 515, 753, 820, 871, 1385
BmrI	ACTGGG	2 cut(s)	628, 1765
BmsI	GCATC	5 cut(s)	418, 562, 1111, 1288, 1567
BmtI	GCTAGC	1 cut(s)	87
BmuI	ACTGGG	2 cut(s)	628, 1765
BpiI	GAAGAC	4 cut(s)	41, 490, 1243, 1440
BplI	GAGNNNNNCTC	2 cut(s)	1401, 1433
BpmI	CTGGAG	1 cut(s)	1378
BpuEI	CTTGAG	2 cut(s)	1308, 1354
BpuMI	CCSGG	4 cut(s)	515, 753, 820, 1385
Bsa29I	ATCGAT	1 cut(s)	279
BsaAI	YACGTR	1 cut(s)	1729
BsaHI	GRCGYC	1 cut(s)	450
BsaI	GGTCTC	1 cut(s)	468
BsaJI	CCNNGG	5 cut(s)	43, 95, 819, 870, 1614
BsaWI	WCCGGW	1 cut(s)	1850
BsaXI	ACNNNNNCTCC	4 cut(s)	131, 161, 448, 478
Bsc4I	CCNNNNNNNGG	8 cut(s)	96, 97, 98, 190, 591, 791, 1591, 1904
Bse118I	RCCGGY	1 cut(s)	295
Bse1I	ACTGG	5 cut(s)	607, 623, 759, 1711, 1760
Bse21I	CCTNAGG	1 cut(s)	189
BseBI	CCWGG	3 cut(s)	152, 243, 871
BseCI	ATCGAT	1 cut(s)	279
BseDI	CCNNGG	5 cut(s)	43, 95, 819, 870, 1614
BseGI	GGATG	8 cut(s)	148, 540, 577, 727, 801, 829, 959, 1582
BseLI	CCNNNNNNNGG	8 cut(s)	96, 97, 98, 190, 591, 791, 1591, 1904
BseNI	ACTGG	5 cut(s)	607, 623, 759, 1711, 1760
BseRI	GAGGAG	1 cut(s)	1086
BseSI	GKGCMC	3 cut(s)	163, 967, 1390
BseXI	GCAGC	5 cut(s)	368, 715, 868, 871, 1897
BseYI	CCCAGC	1 cut(s)	913
BsgI	GTGCAG	2 cut(s)	1314, 1849
Bsh1236I	CGCG	1 cut(s)	1772
BshNI	GGYRCC	2 cut(s)	298, 1387
BshVI	ATCGAT	1 cut(s)	279
BsiHKAI	GWGCWC	2 cut(s)	967, 1193
BsiSI	CCGG	8 cut(s)	296, 515, 576, 753, 819, 1384, 1632, 1851
BslFI	GGGAC	1 cut(s)	349
BslI	CCNNNNNNNGG	8 cut(s)	96, 97, 98, 190, 591, 791, 1591, 1904
BsmAI	GTCTC	2 cut(s)	468, 1059
BsmFI	GGGAC	1 cut(s)	349
BsmI	GAATGC	2 cut(s)	565, 1113
Bso31I	GGTCTC	1 cut(s)	468
Bsp1286I	GDGCHC	4 cut(s)	163, 967, 1193, 1390
Bsp1407I	TGTACA	1 cut(s)	364
Bsp143I	GATC	6 cut(s)	304, 390, 441, 510, 1210, 1339
BspACI	CCGC	4 cut(s)	92, 592, 1770, 1821
BspDI	ATCGAT	1 cut(s)	279
BspFNI	CGCG	1 cut(s)	1772
BspHI	TCATGA	2 cut(s)	399, 549
BspLI	GGNNCC	8 cut(s)	300, 392, 512, 757, 901, 1389, 1612, 1896
BspMAI	CTGCAG	2 cut(s)	705, 1934
BspOI	GCTAGC	1 cut(s)	87
BspPI	GGATC	5 cut(s)	299, 385, 398, 505, 518
BspT107I	GGYRCC	2 cut(s)	298, 1387
BspTNI	GGTCTC	1 cut(s)	468
BsrFI	RCCGGY	1 cut(s)	295
BsrGI	TGTACA	1 cut(s)	364
BsrI	ACTGG	5 cut(s)	607, 623, 759, 1711, 1760
BssAI	RCCGGY	1 cut(s)	295
BssECI	CCNNGG	5 cut(s)	43, 95, 819, 870, 1614
BssMI	GATC	6 cut(s)	304, 390, 441, 510, 1210, 1339
BssNAI	GTATAC	1 cut(s)	1726
BssNI	GRCGYC	1 cut(s)	450
BssT1I	CCWWGG	1 cut(s)	1614
Bst1107I	GTATAC	1 cut(s)	1726
Bst2UI	CCWGG	3 cut(s)	152, 243, 871
Bst4CI	ACNGT	3 cut(s)	812, 948, 983
Bst6I	CTCTTC	1 cut(s)	468
BstACI	GRCGYC	1 cut(s)	450
BstAPI	GCANNNNNTGC	2 cut(s)	428, 709
BstAUI	TGTACA	1 cut(s)	364
BstBAI	YACGTR	1 cut(s)	1729
BstC8I	GCNNGC	7 cut(s)	85, 714, 911, 1505, 1509, 1690, 1819
BstDEI	CTNAG	4 cut(s)	189, 664, 934, 959
BstENI	CCTNNNNNAGG	1 cut(s)	188
BstF5I	GGATG	8 cut(s)	148, 540, 577, 727, 801, 829, 959, 1582
BstFNI	CGCG	1 cut(s)	1772
BstH2I	RGCGCY	1 cut(s)	249
BstHHI	GCGC	4 cut(s)	248, 530, 1643, 1774
BstKTI	GATC	6 cut(s)	307, 393, 444, 513, 1213, 1342
BstMAI	GTCTC	2 cut(s)	468, 1059
BstMBI	GATC	6 cut(s)	304, 390, 441, 510, 1210, 1339
BstMWI	GCNNNNNNNGC	6 cut(s)	428, 581, 709, 1778, 1787, 1827
BstNI	CCWGG	3 cut(s)	152, 243, 871
BstNSI	RCATGY	4 cut(s)	716, 808, 913, 1511
BstSCI	CCNGG	7 cut(s)	150, 241, 513, 751, 818, 869, 1383
BstSFI	CTRYAG	2 cut(s)	701, 1930
BstSLI	GKGCMC	3 cut(s)	163, 967, 1390
BstSNI	TACGTA	1 cut(s)	1729
BstUI	CGCG	1 cut(s)	1772
BstV1I	GCAGC	5 cut(s)	368, 715, 868, 871, 1897
BstV2I	GAAGAC	4 cut(s)	41, 490, 1243, 1440
BstX2I	RGATCY	4 cut(s)	390, 441, 510, 1339
BstXI	CCANNNNNNTGG	3 cut(s)	315, 445, 993
BstYI	RGATCY	4 cut(s)	390, 441, 510, 1339
BstZ17I	GTATAC	1 cut(s)	1726
Bsu15I	ATCGAT	1 cut(s)	279
Bsu36I	CCTNAGG	1 cut(s)	189
BsuTUI	ATCGAT	1 cut(s)	279
BtsCI	GGATG	8 cut(s)	148, 540, 577, 727, 801, 829, 959, 1582
BtsIMutI	CAGTG	3 cut(s)	345, 766, 1677
Cac8I	GCNNGC	7 cut(s)	85, 714, 911, 1505, 1509, 1690, 1819
CaiI	CAGNNNCTG	1 cut(s)	709
CciI	TCATGA	2 cut(s)	399, 549
CfoI	GCGC	4 cut(s)	248, 530, 1643, 1774
Cfr10I	RCCGGY	1 cut(s)	295
Cfr13I	GGNCC	3 cut(s)	983, 1495, 1848
ClaI	ATCGAT	1 cut(s)	279
Csp6I	GTAC	5 cut(s)	226, 365, 414, 671, 808
CviQI	GTAC	5 cut(s)	226, 365, 414, 671, 808
DdeI	CTNAG	4 cut(s)	189, 664, 934, 959
DpnI	GATC	6 cut(s)	306, 392, 443, 512, 1212, 1341
DpnII	GATC	6 cut(s)	304, 390, 441, 510, 1210, 1339
DrdI	GACNNNNNNGTC	1 cut(s)	1039
DseDI	GACNNNNNNGTC	1 cut(s)	1039
EaeI	YGGCCR	4 cut(s)	55, 777, 1047, 1815
Eam1104I	CTCTTC	1 cut(s)	468
EarI	CTCTTC	1 cut(s)	468
Eco105I	TACGTA	1 cut(s)	1729
Eco130I	CCWWGG	1 cut(s)	1614
Eco147I	AGGCCT	1 cut(s)	584
Eco31I	GGTCTC	1 cut(s)	468
Eco32I	GATATC	1 cut(s)	1457
Eco47I	GGWCC	3 cut(s)	983, 1495, 1848
Eco57I	CTGAAG	1 cut(s)	369
Eco81I	CCTNAGG	1 cut(s)	189
EcoNI	CCTNNNNNAGG	1 cut(s)	188
EcoO109I	RGGNCCY	1 cut(s)	1495
EcoRII	CCWGG	3 cut(s)	150, 241, 869
EcoRV	GATATC	1 cut(s)	1457
EcoT14I	CCWWGG	1 cut(s)	1614
ErhI	CCWWGG	1 cut(s)	1614
FalI	AAGNNNNNCTT	2 cut(s)	572, 604
FaqI	GGGAC	1 cut(s)	349
FauI	CCCGC	1 cut(s)	99
FblI	GTMKAC	1 cut(s)	1725
Fnu4HI	GCNGC	5 cut(s)	357, 704, 882, 885, 1911
FokI	GGATG	8 cut(s)	155, 547, 584, 714, 808, 836, 966, 1589
Fsp4HI	GCNGC	5 cut(s)	357, 704, 882, 885, 1911
FspAI	RTGCGCAY	1 cut(s)	529
FspBI	CTAG	2 cut(s)	84, 851
FspI	TGCGCA	2 cut(s)	529, 1642
GlaI	GCGC	4 cut(s)	247, 529, 1642, 1773
GluI	GCNGC	5 cut(s)	357, 704, 882, 885, 1911
GsaI	CCCAGC	1 cut(s)	917
GsuI	CTGGAG	1 cut(s)	1378
HaeII	RGCGCY	1 cut(s)	249
HapII	CCGG	8 cut(s)	296, 515, 576, 753, 819, 1384, 1632, 1851
HhaI	GCGC	4 cut(s)	248, 530, 1643, 1774
Hin1I	GRCGYC	1 cut(s)	450
Hin6I	GCGC	4 cut(s)	246, 528, 1641, 1772
HinP1I	GCGC	4 cut(s)	246, 528, 1641, 1772
HincII	GTYRAC	1 cut(s)	802
HindII	GTYRAC	1 cut(s)	802
HinfI	GANTC	6 cut(s)	116, 546, 833, 1000, 1412, 1652
HpaII	CCGG	8 cut(s)	296, 515, 576, 753, 819, 1384, 1632, 1851
HphI	GGTGA	3 cut(s)	668, 1554, 1630
Hpy166II	GTNNAC	7 cut(s)	345, 416, 802, 965, 986, 1167, 1726
Hpy188I	TCNGA	5 cut(s)	381, 455, 694, 1245, 1917
Hpy188III	TCNNGA	8 cut(s)	308, 388, 400, 550, 1233, 1331, 1343, 1924
Hpy8I	GTNNAC	7 cut(s)	345, 416, 802, 965, 986, 1167, 1726
Hpy99I	CGWCG	2 cut(s)	328, 1903
HpyAV	CCTTC	4 cut(s)	194, 330, 595, 1459
HpyCH4III	ACNGT	3 cut(s)	812, 948, 983
HpyCH4IV	ACGT	3 cut(s)	450, 1542, 1728
HpyF10VI	GCNNNNNNNGC	6 cut(s)	428, 581, 709, 1778, 1787, 1827
HpyF3I	CTNAG	4 cut(s)	189, 664, 934, 959
HpySE526I	ACGT	3 cut(s)	450, 1542, 1728
Hsp92I	GRCGYC	1 cut(s)	450
HspAI	GCGC	4 cut(s)	246, 528, 1641, 1772
Kzo9I	GATC	6 cut(s)	304, 390, 441, 510, 1210, 1339
LmnI	GCTCC	3 cut(s)	186, 899, 1374
Lsp1109I	GCAGC	5 cut(s)	368, 715, 868, 871, 1897
LweI	GCATC	5 cut(s)	418, 562, 1111, 1288, 1567
MaeI	CTAG	2 cut(s)	84, 851
MaeII	ACGT	3 cut(s)	450, 1542, 1728
MaeIII	GTNAC	3 cut(s)	594, 1528, 1675
MalI	GATC	6 cut(s)	306, 392, 443, 512, 1212, 1341
MboI	GATC	6 cut(s)	304, 390, 441, 510, 1210, 1339
MboII	GAAGA	9 cut(s)	31, 41, 244, 485, 495, 1248, 1445, 1673, 1853
MfeI	CAATTG	1 cut(s)	1308
MflI	RGATCY	4 cut(s)	390, 441, 510, 1339
MhlI	GDGCHC	4 cut(s)	163, 967, 1193, 1390
MlsI	TGGCCA	3 cut(s)	779, 1049, 1817
MluNI	TGGCCA	3 cut(s)	779, 1049, 1817
MlyI	GAGTC	1 cut(s)	110
MmeI	TCCRAC	2 cut(s)	83, 549
Mox20I	TGGCCA	3 cut(s)	779, 1049, 1817
MroXI	GAANNNNTTC	1 cut(s)	1651
MscI	TGGCCA	3 cut(s)	779, 1049, 1817
MseI	TTAA	4 cut(s)	1100, 1200, 1400, 1545
MslI	CAYNNNNRTG	4 cut(s)	350, 554, 861, 991
Msp20I	TGGCCA	3 cut(s)	779, 1049, 1817
MspA1I	CMGCKG	1 cut(s)	1821
MspI	CCGG	8 cut(s)	296, 515, 576, 753, 819, 1384, 1632, 1851
MspR9I	CCNGG	7 cut(s)	152, 243, 515, 753, 820, 871, 1385
MunI	CAATTG	1 cut(s)	1308
Mva1269I	GAATGC	2 cut(s)	565, 1113
MvaI	CCWGG	3 cut(s)	152, 243, 871
MvnI	CGCG	1 cut(s)	1772
MwoI	GCNNNNNNNGC	6 cut(s)	428, 581, 709, 1778, 1787, 1827
NciI	CCSGG	4 cut(s)	515, 753, 820, 1385
NdeII	GATC	6 cut(s)	304, 390, 441, 510, 1210, 1339
NheI	GCTAGC	1 cut(s)	83
NlaIV	GGNNCC	8 cut(s)	300, 392, 512, 757, 901, 1389, 1612, 1896
NmeAIII	GCCGAG	4 cut(s)	33, 120, 589, 1257
NmuCI	GTSAC	2 cut(s)	594, 1675
NsbI	TGCGCA	2 cut(s)	529, 1642
NspI	RCATGY	4 cut(s)	716, 808, 913, 1511
OliI	CACNNNNGTG	1 cut(s)	350
PaeI	GCATGC	3 cut(s)	716, 913, 1511
PagI	TCATGA	2 cut(s)	399, 549
PceI	AGGCCT	1 cut(s)	584
PciI	ACATGT	1 cut(s)	804
PcsI	WCGNNNNNNNCGW	1 cut(s)	116
PctI	GAATGC	2 cut(s)	565, 1113
PdmI	GAANNNNTTC	1 cut(s)	1651
PfeI	GAWTC	5 cut(s)	546, 833, 1000, 1412, 1652
PfoI	TCCNGGA	3 cut(s)	150, 513, 751
PkrI	GCNGC	5 cut(s)	358, 705, 883, 886, 1912
PleI	GAGTC	1 cut(s)	110
PpsI	GAGTC	1 cut(s)	110
Ppu21I	YACGTR	1 cut(s)	1729
PpuMI	RGGWCCY	1 cut(s)	1495
PscI	ACATGT	1 cut(s)	804
PshBI	ATTAAT	1 cut(s)	1100
Psp5II	RGGWCCY	1 cut(s)	1495
Psp6I	CCWGG	3 cut(s)	150, 241, 869
PspFI	CCCAGC	1 cut(s)	913
PspGI	CCWGG	3 cut(s)	150, 241, 869
PspN4I	GGNNCC	8 cut(s)	300, 392, 512, 757, 901, 1389, 1612, 1896
PspPI	GGNCC	3 cut(s)	983, 1495, 1848
PspPPI	RGGWCCY	1 cut(s)	1495
PstI	CTGCAG	2 cut(s)	705, 1934
PstNI	CAGNNNCTG	1 cut(s)	709
PsuI	RGATCY	4 cut(s)	390, 441, 510, 1339
RsaI	GTAC	5 cut(s)	227, 366, 415, 672, 809
RsaNI	GTAC	5 cut(s)	226, 365, 414, 671, 808
RseI	CAYNNNNRTG	4 cut(s)	350, 554, 861, 991
SaqAI	TTAA	4 cut(s)	1100, 1200, 1400, 1545
SatI	GCNGC	5 cut(s)	357, 704, 882, 885, 1911
Sau3AI	GATC	6 cut(s)	304, 390, 441, 510, 1210, 1339
Sau96I	GGNCC	3 cut(s)	983, 1495, 1848
SchI	GAGTC	1 cut(s)	110
ScrFI	CCNGG	7 cut(s)	152, 243, 515, 753, 820, 871, 1385
SduI	GDGCHC	4 cut(s)	163, 967, 1193, 1390
SfaNI	GCATC	5 cut(s)	418, 562, 1111, 1288, 1567
SfcI	CTRYAG	2 cut(s)	701, 1930
SinI	GGWCC	3 cut(s)	983, 1495, 1848
SmiMI	CAYNNNNRTG	4 cut(s)	350, 554, 861, 991
SmlI	CTYRAG	2 cut(s)	1323, 1369
SmoI	CTYRAG	2 cut(s)	1323, 1369
SnaBI	TACGTA	1 cut(s)	1729
SphI	GCATGC	3 cut(s)	716, 913, 1511
SseBI	AGGCCT	1 cut(s)	584
SsiI	CCGC	4 cut(s)	92, 592, 1770, 1821
SspMI	CTAG	2 cut(s)	84, 851
StuI	AGGCCT	1 cut(s)	584
StyD4I	CCNGG	7 cut(s)	150, 241, 513, 751, 818, 869, 1383
StyI	CCWWGG	1 cut(s)	1614
TaaI	ACNGT	3 cut(s)	812, 948, 983
TaiI	ACGT	3 cut(s)	453, 1545, 1731
TaqI	TCGA	4 cut(s)	279, 326, 998, 1213
TaqII	GACCGA	3 cut(s)	35, 365, 1350
TatI	WGTACW	2 cut(s)	364, 413
TfiI	GAWTC	5 cut(s)	546, 833, 1000, 1412, 1652
Tru1I	TTAA	4 cut(s)	1100, 1200, 1400, 1545
Tru9I	TTAA	4 cut(s)	1100, 1200, 1400, 1545
TscAI	CASTG	3 cut(s)	352, 766, 1684
TseFI	GTSAC	2 cut(s)	594, 1675
TseI	GCWGC	5 cut(s)	356, 703, 881, 884, 1910
Tsp45I	GTSAC	2 cut(s)	594, 1675
TspGWI	ACGGA	3 cut(s)	152, 1457, 1887
TspRI	CASTG	3 cut(s)	352, 766, 1684
VneI	GTGCAC	1 cut(s)	963
VpaK11BI	GGWCC	3 cut(s)	983, 1495, 1848
VspI	ATTAAT	1 cut(s)	1100
XagI	CCTNNNNNAGG	1 cut(s)	188
XapI	RAATTY	2 cut(s)	383, 1138
XceI	RCATGY	4 cut(s)	716, 808, 913, 1511
XcmI	CCANNNNNNNNNTGG	2 cut(s)	910, 1612
XmiI	GTMKAC	1 cut(s)	1725
XmnI	GAANNNNTTC	1 cut(s)	1651
XspI	CTAG	2 cut(s)	84, 851
ZraI	GACGTC	1 cut(s)	451

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.