Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012236

Physical Location & Seq

Genomic Coordinates Forward (+)

6916689 .. 6921132

Gene Structure 4444 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1099400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1929 bp

ATGAATGTCTGCTTGTTCCTCTTCACCTCTCTCTGCTTCTTACTCGGTCTGTTCACCCAGAGGGTGTCATCACAAGATCGCGCCAGCACCCCGCCGAGAGGTTGGAACTCGTATGACTCGTTTATCTGGACTATTTCAGAGGAAGAATTCTCCCAAAATGCCAAAATTATGGCAAAGCAGCTCCTTCCTCATGGATATGAGTATGCTGTGGTGGATTACCTGTGGTATAGGAGAAAAGTTCCAGGTGCTTATGTTGATTCTCTTGGGTTTGATGTGATCGATGAGTGGGGGAGGCCGATACCTGATCCAGATAGGTGGCCTTCGTCGAGAGGTGGGAAAGGGTTCGCTGAAGTGGCAGCAAAAGTACATAGCCTCGGTTTGAAATTCGGGATTCATGTCATGAGAGGAATAAGTACACAGGCAGTAAATGCGAGTACCTTGATCTTGGACACCAACACGGGGTCTGCTTACAAAGAGGCTGGAAGAAATTGGACTGCAAAAGATATAGGGATCAAGGAGAGGACTTGTGCATGGATGCACAATGGTTTCATGAGTGTGAATACCAAGTTGGGCGCTGGAAAGGCCTTCTTGAGGTCACTCTATAAACAGTATGCTGAATGGGGTGTTGATTTTGTGAAAAATGACTGCGTGTTTGGTGAAGACTTAGACGTACCCGAAATAACCTACATTTCAGACATTCTGAAGCAACTTGACCGTCCTATATTGTATTCTCTATCTCCTGGAACCAGAGTAACACCTGATATGGCCAAGGCTATAAGTGGAATGGTCAACATGTACCGTATAACTGGGGATGATTGGGATTCATGGGGAGATGTTCTAGCCCATTTTGATGTCACCAGGGATTTTGCTGCTGCTGATATGATAGGAGCCAAAGGCTTGCTGGGGAAGTCATGGCCGGACTTAGATATGCTTCCGTTGGGATGGCTTAGTGATCCAGGTTCAAATGACGGTCCACACAGATGGACTAATCTCGGTTTAAGGGAGCAAAGAACGCAGATGACTTTGTGGTCTATGGCCAGGTCTCCTCTCATGTTTGGAGGGGATATGAGACAGCTTAATGATGTCACAATGGAACTCCTTGCAAACCCTACCGTGCTGGAGATAAATTCTTTTAGCTCAAATAATAGGGAGTTTTCATACATTACAAGTAATGTGAGTGCAAAGGCCGTTAAGAATGATGATCACGTTCACACCTGGCAATCAAGAAGACATCTGACAAATGCAAAAACTACAAATACACATGTTCTTGGTCTCACTACCTGTGCAGATGCAAAAGCAATTGGTTGGTCCTCTCAAGCTCTTGACCAAGATCGCGAACACATCTGCTGGACGGCAAACTCAAGGGGCAAACATCAGGCACCTTTTTGCTTAAACAAGACAGAATCTCTCCTGGCAATAGATGAAGACATAACGGACAAACAGCAATATCTGGGGAAGGTCAATTTATTAGCAAATGATGGAACGGATCGCTGTTTGCATGCTTCTCCTAAACAAAAGCTTACTTCGAAGGACGTTAAGAGAGGTTTCCTTTCACCTTGTAGATGGGATGCAAACCAGATGTGGGAGTTGAACCCCAACGGAACCTTGGTGAATAGTTATTCTGGCCTCTGTGCAAGAATTGATTCTTTATTTGAAGCTAAAGCCGTCACTGATGTTGCTCGCTCTTGGATTGCAACTGGAACAAAAGGAGAAATATACATAGCGTTTTTCAATTTGTTCAGTGATTGGGCTACTATAACTGCACAAAGAGCAGACATAGTCAAAGAACTTCCAATGACAAAATTGGCCAGAGGTTCTTGCACGGGATCGGAAGTATGGACCGGACAAGACTTAGGGGTAATAGGCGACGCAATTTCTTTTTCGGTTATACCGCACGGGGTTGCACTGATTGTCCTGCACTGTAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004557 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005994 GO:0005995 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006672 GO:0006677 GO:0006687 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009311 GO:0009313 GO:0009987 GO:0015925 GO:0016042 GO:0016052 GO:0016137 GO:0016139 GO:0016787 GO:0016798 GO:0019377 GO:0030149 GO:0034641 GO:0043603 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044262 GO:0044275 GO:0044424 GO:0044464 GO:0046352 GO:0046466 GO:0046477 GO:0046479 GO:0046514 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1901657 GO:1901658 GO:1903509

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Melibiase_2

Protein Analysis

643

Amino Acids

71.52

Weight (kDa)

6.33

Isoelectric Point (pI)

30.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Melibiase_2	PF16499	202 - 376	1.9e-12	Alpha galactosidase A
Melibiase_C	PF17801	562 - 639	8.2e-07	Alpha galactosidase C-terminal beta sandwich domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012236)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G26380 AT3G26380
fragaria_vesca	FvH4_4g25930
malus_domestica	MD13G1098300.v1.1 MD13G1098400.v1.1 MD16G1099400.v1.1
prunus_persica	Prupe.1G247000_v2.0.a1
pyrus_communis	pycom13g08600 pycom16g08460
rosa_chinensis	RchiOBHm_Chr4g0433401
rosa_laevigata	RLG00000006756
rosa_multiflora	Rmu_sc0000759.1_g000006
rosa_roxburghii	Rroxscaffold_5G00374950
rosa_rugosa	Rorug04G0268500
rosa_samantha	Rh4AG323300 Rh4BG331000 Rh4CG346300 Rh4DG327400
rosa_wichuraiana	Rw4G028000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1027
AccB1I	GGYRCC	1 cut(s)	1378
AccII	CGCG	2 cut(s)	81, 1335
AciI	CCGC	2 cut(s)	92, 1892
AclWI	GGATC	5 cut(s)	299, 518, 947, 1494, 1834
AcoI	YGGCCR	4 cut(s)	765, 914, 1035, 1806
AcsI	RAATTY	3 cut(s)	146, 383, 1126
AcuI	CTGAAG	2 cut(s)	369, 722
AfaI	GTAC	5 cut(s)	366, 415, 436, 672, 797
AfiI	CCNNNNNNNGG	4 cut(s)	98, 459, 591, 1582
AflIII	ACRYGT	2 cut(s)	792, 1261
AgsI	TTSAA	5 cut(s)	382, 963, 1591, 1655, 1732
AjnI	CCWGG	7 cut(s)	241, 739, 857, 955, 1037, 1214, 1410
AjuI	GAANNNNNNNTTGG	2 cut(s)	551, 583
AluBI	AGCT	6 cut(s)	181, 1075, 1137, 1319, 1519, 1658
AluI	AGCT	6 cut(s)	181, 1075, 1137, 1319, 1519, 1658
Alw26I	GTCTC	3 cut(s)	1047, 1063, 1277
AlwI	GGATC	5 cut(s)	299, 518, 947, 1494, 1834
AoxI	GGCC	9 cut(s)	293, 317, 582, 765, 914, 1035, 1185, 1624, 1806
ApeKI	GCWGC	4 cut(s)	178, 356, 869, 872
ApoI	RAATTY	3 cut(s)	146, 383, 1126
ArsI	GACNNNNNNTTYG	2 cut(s)	635, 667
Asp700I	GAANNNNTTC	2 cut(s)	341, 1642
AspLEI	GCGC	2 cut(s)	83, 575
AspS9I	GGNCC	3 cut(s)	971, 1308, 1839
AsuHPI	GGTGA	6 cut(s)	16, 46, 668, 847, 1545, 1621
AsuII	TTCGAA	1 cut(s)	1526
AvaII	GGWCC	3 cut(s)	971, 1308, 1839
BalI	TGGCCA	3 cut(s)	767, 1037, 1808
BanI	GGYRCC	1 cut(s)	1378
BbsI	GAAGAC	3 cut(s)	666, 1234, 1431
BbvI	GCAGC	4 cut(s)	190, 368, 856, 859
BccI	CCATC	4 cut(s)	936, 975, 1472, 1557
BceAI	ACGGC	3 cut(s)	1172, 1368, 1649
BciT130I	CCWGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
BclI	TGATCA	1 cut(s)	1201
BcoDI	GTCTC	3 cut(s)	1047, 1063, 1277
BfaI	CTAG	1 cut(s)	839
BfmI	CTRYAG	1 cut(s)	1921
BfoI	RGCGCY	1 cut(s)	576
BisI	GCNGC	4 cut(s)	179, 357, 870, 873
BlsI	GCNGC	4 cut(s)	180, 358, 871, 874
Bme1390I	CCNGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
Bme18I	GGWCC	3 cut(s)	971, 1308, 1839
BmgT120I	GGNCC	3 cut(s)	971, 1308, 1839
BmiI	GGNNCC	4 cut(s)	745, 889, 1380, 1603
BmrFI	CCNGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
BmrI	ACTGGG	1 cut(s)	816
BmsI	GCATC	3 cut(s)	525, 1279, 1558
BmuI	ACTGGG	1 cut(s)	816
BpiI	GAAGAC	3 cut(s)	666, 1234, 1431
BpmI	CTGGAG	1 cut(s)	1139
Bpu14I	TTCGAA	1 cut(s)	1526
BpuEI	CTTGAG	3 cut(s)	610, 1299, 1345
Bsa29I	ATCGAT	1 cut(s)	279
BsaI	GGTCTC	2 cut(s)	1047, 1277
BsaJI	CCNNGG	4 cut(s)	373, 768, 858, 1605
BsaWI	WCCGGW	1 cut(s)	1841
Bsc4I	CCNNNNNNNGG	4 cut(s)	98, 459, 591, 1582
Bse1I	ACTGG	2 cut(s)	811, 1702
BseBI	CCWGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
BseCI	ATCGAT	1 cut(s)	279
BseDI	CCNNGG	4 cut(s)	373, 768, 858, 1605
BseGI	GGATG	4 cut(s)	540, 817, 947, 1573
BseLI	CCNNNNNNNGG	4 cut(s)	98, 459, 591, 1582
BseNI	ACTGG	2 cut(s)	811, 1702
BseRI	GAGGAG	1 cut(s)	1035
BseXI	GCAGC	4 cut(s)	190, 368, 856, 859
BseYI	CCCAGC	1 cut(s)	901
BsgI	GTGCAG	3 cut(s)	1305, 1746, 1901
Bsh1236I	CGCG	2 cut(s)	81, 1335
BshFI	GGCC	9 cut(s)	295, 319, 584, 767, 916, 1037, 1187, 1626, 1808
BshNI	GGYRCC	1 cut(s)	1378
BshVI	ATCGAT	1 cut(s)	279
BsiSI	CCGG	2 cut(s)	917, 1842
BslI	CCNNNNNNNGG	4 cut(s)	98, 459, 591, 1582
BsmAI	GTCTC	3 cut(s)	1047, 1063, 1277
BsnI	GGCC	9 cut(s)	295, 319, 584, 767, 916, 1037, 1187, 1626, 1808
Bso31I	GGTCTC	2 cut(s)	1047, 1277
Bsp119I	TTCGAA	1 cut(s)	1526
Bsp68I	TCGCGA	1 cut(s)	1335
BspACI	CCGC	2 cut(s)	92, 1892
BspANI	GGCC	9 cut(s)	295, 319, 584, 767, 916, 1037, 1187, 1626, 1808
BspDI	ATCGAT	1 cut(s)	279
BspFNI	CGCG	2 cut(s)	81, 1335
BspHI	TCATGA	2 cut(s)	399, 549
BspLI	GGNNCC	4 cut(s)	745, 889, 1380, 1603
BspPI	GGATC	5 cut(s)	299, 518, 947, 1494, 1834
BspT104I	TTCGAA	1 cut(s)	1526
BspT107I	GGYRCC	1 cut(s)	1378
BspTNI	GGTCTC	2 cut(s)	1047, 1277
BsrI	ACTGG	2 cut(s)	811, 1702
BssECI	CCNNGG	4 cut(s)	373, 768, 858, 1605
BssT1I	CCWWGG	2 cut(s)	768, 1605
Bst2UI	CCWGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
Bst4CI	ACNGT	6 cut(s)	609, 716, 800, 971, 1114, 1922
Bst6I	CTCTTC	1 cut(s)	26
BstAPI	GCANNNNNTGC	1 cut(s)	428
BstBI	TTCGAA	1 cut(s)	1526
BstC8I	GCNNGC	4 cut(s)	85, 899, 1500, 1681
BstDEI	CTNAG	4 cut(s)	664, 922, 947, 1852
BstENI	CCTNNNNNAGG	1 cut(s)	589
BstF5I	GGATG	4 cut(s)	540, 817, 947, 1573
BstFNI	CGCG	2 cut(s)	81, 1335
BstH2I	RGCGCY	1 cut(s)	576
BstHHI	GCGC	2 cut(s)	83, 575
BstMAI	GTCTC	3 cut(s)	1047, 1063, 1277
BstMWI	GCNNNNNNNGC	5 cut(s)	353, 428, 581, 1012, 1769
BstNI	CCWGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
BstNSI	RCATGY	3 cut(s)	796, 1265, 1502
BstSCI	CCNGG	7 cut(s)	241, 739, 857, 955, 1037, 1214, 1410
BstSFI	CTRYAG	1 cut(s)	1921
BstUI	CGCG	2 cut(s)	81, 1335
BstV1I	GCAGC	4 cut(s)	190, 368, 856, 859
BstV2I	GAAGAC	3 cut(s)	666, 1234, 1431
BstXI	CCANNNNNNTGG	3 cut(s)	169, 315, 981
Bsu15I	ATCGAT	1 cut(s)	279
BsuRI	GGCC	9 cut(s)	295, 319, 584, 767, 916, 1037, 1187, 1626, 1808
BsuTUI	ATCGAT	1 cut(s)	279
BtsCI	GGATG	4 cut(s)	540, 817, 947, 1573
BtsIMutI	CAGTG	4 cut(s)	1668, 1747, 1904, 1918
BtuMI	TCGCGA	1 cut(s)	1335
Cac8I	GCNNGC	4 cut(s)	85, 899, 1500, 1681
CciI	TCATGA	2 cut(s)	399, 549
CfoI	GCGC	2 cut(s)	83, 575
Cfr13I	GGNCC	3 cut(s)	971, 1308, 1839
ClaI	ATCGAT	1 cut(s)	279
CseI	GACGC	1 cut(s)	1877
Csp6I	GTAC	5 cut(s)	365, 414, 435, 671, 796
CviQI	GTAC	5 cut(s)	365, 414, 435, 671, 796
DdeI	CTNAG	4 cut(s)	664, 922, 947, 1852
DrdI	GACNNNNNNGTC	1 cut(s)	1027
DseDI	GACNNNNNNGTC	1 cut(s)	1027
EaeI	YGGCCR	4 cut(s)	765, 914, 1035, 1806
Eam1104I	CTCTTC	1 cut(s)	26
EarI	CTCTTC	1 cut(s)	26
Eco130I	CCWWGG	2 cut(s)	768, 1605
Eco147I	AGGCCT	1 cut(s)	584
Eco31I	GGTCTC	2 cut(s)	1047, 1277
Eco47I	GGWCC	3 cut(s)	971, 1308, 1839
Eco57I	CTGAAG	2 cut(s)	369, 722
EcoNI	CCTNNNNNAGG	1 cut(s)	589
EcoRI	GAATTC	1 cut(s)	146
EcoRII	CCWGG	7 cut(s)	241, 739, 857, 955, 1037, 1214, 1410
EcoT14I	CCWWGG	2 cut(s)	768, 1605
ErhI	CCWWGG	2 cut(s)	768, 1605
FalI	AAGNNNNNCTT	2 cut(s)	572, 604
FauI	CCCGC	1 cut(s)	99
FbaI	TGATCA	1 cut(s)	1201
Fnu4HI	GCNGC	4 cut(s)	179, 357, 870, 873
FokI	GGATG	4 cut(s)	547, 824, 954, 1580
Fsp4HI	GCNGC	4 cut(s)	179, 357, 870, 873
FspBI	CTAG	1 cut(s)	839
GlaI	GCGC	2 cut(s)	82, 574
GluI	GCNGC	4 cut(s)	179, 357, 870, 873
GsaI	CCCAGC	1 cut(s)	905
GsuI	CTGGAG	1 cut(s)	1139
HaeII	RGCGCY	1 cut(s)	576
HaeIII	GGCC	9 cut(s)	295, 319, 584, 767, 916, 1037, 1187, 1626, 1808
HapII	CCGG	2 cut(s)	917, 1842
HgaI	GACGC	1 cut(s)	1877
HhaI	GCGC	2 cut(s)	83, 575
Hin6I	GCGC	2 cut(s)	81, 573
HinP1I	GCGC	2 cut(s)	81, 573
HincII	GTYRAC	1 cut(s)	790
HindII	GTYRAC	1 cut(s)	790
HindIII	AAGCTT	1 cut(s)	1517
HinfI	GANTC	6 cut(s)	116, 257, 391, 821, 1403, 1643
HpaII	CCGG	2 cut(s)	917, 1842
HphI	GGTGA	6 cut(s)	16, 46, 668, 847, 1545, 1621
Hpy166II	GTNNAC	5 cut(s)	54, 416, 790, 974, 1210
Hpy188I	TCNGA	5 cut(s)	139, 694, 702, 1236, 1831
Hpy8I	GTNNAC	5 cut(s)	54, 416, 790, 974, 1210
Hpy99I	CGWCG	2 cut(s)	328, 1871
HpyAV	CCTTC	5 cut(s)	194, 330, 595, 1450, 1522
HpyCH4III	ACNGT	6 cut(s)	609, 716, 800, 971, 1114, 1922
HpyCH4IV	ACGT	3 cut(s)	669, 1206, 1533
HpyF10VI	GCNNNNNNNGC	5 cut(s)	353, 428, 581, 1012, 1769
HpyF3I	CTNAG	4 cut(s)	664, 922, 947, 1852
HpySE526I	ACGT	3 cut(s)	669, 1206, 1533
HspAI	GCGC	2 cut(s)	81, 573
Ksp22I	TGATCA	1 cut(s)	1201
LmnI	GCTCC	3 cut(s)	186, 887, 1003
Lsp1109I	GCAGC	4 cut(s)	190, 368, 856, 859
LweI	GCATC	3 cut(s)	525, 1279, 1558
MaeI	CTAG	1 cut(s)	839
MaeII	ACGT	3 cut(s)	669, 1206, 1533
MaeIII	GTNAC	5 cut(s)	594, 751, 853, 1084, 1666
MboII	GAAGA	6 cut(s)	13, 155, 495, 671, 1239, 1436
MfeI	CAATTG	1 cut(s)	1299
MlsI	TGGCCA	3 cut(s)	767, 1037, 1808
MluNI	TGGCCA	3 cut(s)	767, 1037, 1808
MlyI	GAGTC	1 cut(s)	110
MmeI	TCCRAC	1 cut(s)	83
Mox20I	TGGCCA	3 cut(s)	767, 1037, 1808
MroXI	GAANNNNTTC	2 cut(s)	341, 1642
MscI	TGGCCA	3 cut(s)	767, 1037, 1808
MseI	TTAA	5 cut(s)	998, 1077, 1191, 1391, 1536
MslI	CAYNNNNRTG	4 cut(s)	195, 554, 849, 979
Msp20I	TGGCCA	3 cut(s)	767, 1037, 1808
MspI	CCGG	2 cut(s)	917, 1842
MspR9I	CCNGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
MunI	CAATTG	1 cut(s)	1299
MvaI	CCWGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
MvnI	CGCG	2 cut(s)	81, 1335
MwoI	GCNNNNNNNGC	5 cut(s)	353, 428, 581, 1012, 1769
NlaIV	GGNNCC	4 cut(s)	745, 889, 1380, 1603
NmeAIII	GCCGAG	1 cut(s)	120
NmuCI	GTSAC	4 cut(s)	594, 853, 1084, 1666
NruI	TCGCGA	1 cut(s)	1335
NspI	RCATGY	3 cut(s)	796, 1265, 1502
NspV	TTCGAA	1 cut(s)	1526
PaeI	GCATGC	1 cut(s)	1502
PagI	TCATGA	2 cut(s)	399, 549
PceI	AGGCCT	1 cut(s)	584
PciI	ACATGT	2 cut(s)	792, 1261
PcsI	WCGNNNNNNNCGW	1 cut(s)	116
PdmI	GAANNNNTTC	2 cut(s)	341, 1642
PfeI	GAWTC	5 cut(s)	257, 391, 821, 1403, 1643
PflFI	GACNNNGTC	1 cut(s)	1778
PfoI	TCCNGGA	1 cut(s)	739
PkrI	GCNGC	4 cut(s)	180, 358, 871, 874
PleI	GAGTC	1 cut(s)	110
PpsI	GAGTC	1 cut(s)	110
PscI	ACATGT	2 cut(s)	792, 1261
Psp6I	CCWGG	7 cut(s)	241, 739, 857, 955, 1037, 1214, 1410
PspFI	CCCAGC	1 cut(s)	901
PspGI	CCWGG	7 cut(s)	241, 739, 857, 955, 1037, 1214, 1410
PspN4I	GGNNCC	4 cut(s)	745, 889, 1380, 1603
PspPI	GGNCC	3 cut(s)	971, 1308, 1839
PsyI	GACNNNGTC	1 cut(s)	1778
RruI	TCGCGA	1 cut(s)	1335
RsaI	GTAC	5 cut(s)	366, 415, 436, 672, 797
RsaNI	GTAC	5 cut(s)	365, 414, 435, 671, 796
RseI	CAYNNNNRTG	4 cut(s)	195, 554, 849, 979
SaqAI	TTAA	5 cut(s)	998, 1077, 1191, 1391, 1536
SatI	GCNGC	4 cut(s)	179, 357, 870, 873
Sau96I	GGNCC	3 cut(s)	971, 1308, 1839
SchI	GAGTC	1 cut(s)	110
ScrFI	CCNGG	7 cut(s)	243, 741, 859, 957, 1039, 1216, 1412
SfaNI	GCATC	3 cut(s)	525, 1279, 1558
SfcI	CTRYAG	1 cut(s)	1921
SfuI	TTCGAA	1 cut(s)	1526
SinI	GGWCC	3 cut(s)	971, 1308, 1839
SmiMI	CAYNNNNRTG	4 cut(s)	195, 554, 849, 979
SmlI	CTYRAG	3 cut(s)	589, 1314, 1360
SmoI	CTYRAG	3 cut(s)	589, 1314, 1360
SphI	GCATGC	1 cut(s)	1502
SseBI	AGGCCT	1 cut(s)	584
SsiI	CCGC	2 cut(s)	92, 1892
SspMI	CTAG	1 cut(s)	839
StuI	AGGCCT	1 cut(s)	584
StyD4I	CCNGG	7 cut(s)	241, 739, 857, 955, 1037, 1214, 1410
StyI	CCWWGG	2 cut(s)	768, 1605
TaaI	ACNGT	6 cut(s)	609, 716, 800, 971, 1114, 1922
TaiI	ACGT	3 cut(s)	672, 1209, 1536
TaqI	TCGA	3 cut(s)	279, 326, 1526
TaqII	GACCGA	1 cut(s)	35
TatI	WGTACW	2 cut(s)	364, 413
TfiI	GAWTC	5 cut(s)	257, 391, 821, 1403, 1643
Tru1I	TTAA	5 cut(s)	998, 1077, 1191, 1391, 1536
Tru9I	TTAA	5 cut(s)	998, 1077, 1191, 1391, 1536
TscAI	CASTG	4 cut(s)	1675, 1747, 1911, 1925
TseFI	GTSAC	4 cut(s)	594, 853, 1084, 1666
TseI	GCWGC	4 cut(s)	178, 356, 869, 872
Tsp45I	GTSAC	4 cut(s)	594, 853, 1084, 1666
TspDTI	ATGAA	6 cut(s)	17, 383, 538, 813, 1146, 1437
TspGWI	ACGGA	4 cut(s)	924, 1448, 1499, 1614
TspRI	CASTG	4 cut(s)	1675, 1747, 1911, 1925
Tth111I	GACNNNGTC	1 cut(s)	1778
VpaK11BI	GGWCC	3 cut(s)	971, 1308, 1839
XagI	CCTNNNNNAGG	1 cut(s)	589
XapI	RAATTY	3 cut(s)	146, 383, 1126
XceI	RCATGY	3 cut(s)	796, 1265, 1502
XcmI	CCANNNNNNNNNTGG	2 cut(s)	898, 1603
XmnI	GAANNNNTTC	2 cut(s)	341, 1642
XspI	CTAG	1 cut(s)	839

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.