FvH4_4g26750

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016650
Physical Location & Seq
Reverse (-)
28028254 .. 28029461
1208 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26750.t1

Sequence Viewer

Length: 576 bp
ATGTCTACTCTTCATGCTATTCTTGGAGGAGGAAAAGTGGCTGACATAATGCTGTGGAAGGACAAGAAATCAGCTGGTGCAATCTTATTTGGGTTCACCACATTATGGTTTTTTCTACAAGTACGAGAATACCAATTTGTATCTCTTCTTTGTGACACCCTCATGCTGACTATGACTCTTGGACTCCTCTGGTGCAAATTCGCAGGACGAATACTCAATAGGATGCCACGTGAGATCAACATTCAACTTCCAGAATCTACTTGCATCTACTTCATTGGAACAATTAACTGGTTCCTCTTGAAGTTAAGCGAAATCACATCTGGGAAAGACTTCAAACTCTTCTTTGTGGCATTGGCTGGTCTATACATGATGTCAACTATAGGAACTTATATCAGCTCATGGAACCTAATCTATACAGTCATTCTTGCGGTAGGAACATTGCCAGCTTTGTATGCAAGATATGAAACTCAGGTTGATACAATCGTAGCCAGATGCATCAGTGGCGTGAAGAGATTATACGAATTGTTTCAGGTCAAAATTCTTGACAAGATTCCCAGGCACAGAAAATCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

192

Amino Acids

21.83

Weight (kDa)

9.51

Isoelectric Point (pI)

31.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 13 - 163 1.8e-35 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68230 AT1G68230
fragaria_vesca FvH4_4g26750
malus_domestica MD16G1037300.v1.1
prunus_persica Prupe.1G316500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0434431
rosa_laevigata RLG00000006672
rosa_multiflora Rmu_co8483911.1_g000001
rosa_roxburghii Rroxscaffold_5G00375790
rosa_samantha Rh4AG332100 Rh4BG340000 Rh4CG355200 Rh4DG335300
rosa_wichuraiana Rw4G028800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 105
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 428
AcsI RAATTY 2 cut(s) 197, 537
AcvI CACGTG 1 cut(s) 230
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 1 cut(s) 105
AgsI TTSAA 3 cut(s) 245, 301, 334
AjnI CCWGG 1 cut(s) 554
AluBI AGCT 3 cut(s) 74, 396, 446
AluI AGCT 3 cut(s) 74, 396, 446
ApoI RAATTY 2 cut(s) 197, 537
Asp700I GAANNNNTTC 2 cut(s) 329, 525
AsuHPI GGTGA 1 cut(s) 88
BaeI ACNNNNGTAYC 2 cut(s) 468, 501
BarI GAAGNNNNNNTAC 2 cut(s) 500, 532
BbrPI CACGTG 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 556
BfmI CTRYAG 1 cut(s) 378
Bme1390I CCNGG 1 cut(s) 556
BmiI GGNNCC 2 cut(s) 293, 404
BmrFI CCNGG 1 cut(s) 556
BmsI GCATC 4 cut(s) 213, 273, 482, 504
BsaAI YACGTR 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 554
BsaXI ACNNNNNCTCC 2 cut(s) 21, 51
Bsc4I CCNNNNNNNGG 1 cut(s) 105
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 437
BseBI CCWGG 1 cut(s) 556
BseDI CCNNGG 1 cut(s) 554
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMI GCAATG 1 cut(s) 437
BseMII CTCAG 1 cut(s) 482
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 2 cut(s) 42, 176
BslI CCNNNNNNNGG 1 cut(s) 105
Bsp143I GATC 1 cut(s) 234
BspACI CCGC 1 cut(s) 428
BspCNI CTCAG 1 cut(s) 481
BspLI GGNNCC 2 cut(s) 293, 404
BsrDI GCAATG 1 cut(s) 437
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 1 cut(s) 554
BssMI GATC 1 cut(s) 234
Bst2UI CCWGG 1 cut(s) 556
Bst4CI ACNGT 1 cut(s) 418
Bst6I CTCTTC 4 cut(s) 15, 150, 344, 503
BstBAI YACGTR 1 cut(s) 230
BstC8I GCNNGC 1 cut(s) 444
BstDEI CTNAG 1 cut(s) 468
BstF5I GGATG 1 cut(s) 228
BstKTI GATC 1 cut(s) 237
BstMBI GATC 1 cut(s) 234
BstMWI GCNNNNNNNGC 2 cut(s) 452, 501
BstNI CCWGG 1 cut(s) 556
BstSCI CCNGG 1 cut(s) 554
BstSFI CTRYAG 1 cut(s) 378
BtsCI GGATG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 444
Csp6I GTAC 1 cut(s) 122
CviAII CATG 4 cut(s) 14, 163, 367, 399
CviJI RGCY 6 cut(s) 41, 74, 356, 396, 446, 488
CviKI_1 RGCY 6 cut(s) 41, 74, 356, 396, 446, 488
CviQI GTAC 1 cut(s) 122
DdeI CTNAG 1 cut(s) 468
DpnI GATC 1 cut(s) 236
DpnII GATC 1 cut(s) 234
Eam1104I CTCTTC 4 cut(s) 15, 150, 344, 503
EarI CTCTTC 4 cut(s) 15, 150, 344, 503
Eco72I CACGTG 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 554
EcoT22I ATGCAT 1 cut(s) 497
FaeI CATG 4 cut(s) 17, 166, 370, 402
FatI CATG 4 cut(s) 13, 162, 366, 398
FblI GTMKAC 1 cut(s) 5
FokI GGATG 1 cut(s) 235
Hin1II CATG 4 cut(s) 17, 166, 370, 402
HincII GTYRAC 1 cut(s) 375
HindII GTYRAC 1 cut(s) 375
HinfI GANTC 4 cut(s) 175, 183, 254, 550
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 3 cut(s) 6, 96, 375
Hpy188III TCNNGA 3 cut(s) 251, 298, 542
Hpy8I GTNNAC 3 cut(s) 6, 96, 375
HpyAV CCTTC 1 cut(s) 52
HpyCH4III ACNGT 1 cut(s) 418
HpyCH4IV ACGT 1 cut(s) 229
HpyCH4V TGCA 5 cut(s) 80, 195, 264, 455, 495
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 501
HpyF3I CTNAG 1 cut(s) 468
HpySE526I ACGT 1 cut(s) 229
Hsp92II CATG 4 cut(s) 17, 166, 370, 402
Kzo9I GATC 1 cut(s) 234
LweI GCATC 4 cut(s) 213, 273, 482, 504
MaeII ACGT 1 cut(s) 229
MaeIII GTNAC 1 cut(s) 152
MalI GATC 1 cut(s) 236
MboI GATC 1 cut(s) 234
MboII GAAGA 3 cut(s) 137, 331, 520
MluCI AATT 6 cut(s) 134, 197, 282, 521, 537, 571
MlyI GAGTC 2 cut(s) 169, 177
MnlI CCTC 5 cut(s) 20, 23, 170, 197, 305
Mph1103I ATGCAT 1 cut(s) 497
MroXI GAANNNNTTC 2 cut(s) 329, 525
MseI TTAA 2 cut(s) 285, 305
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 556
MvaI CCWGG 1 cut(s) 556
MwoI GCNNNNNNNGC 2 cut(s) 452, 501
NdeII GATC 1 cut(s) 234
NlaIII CATG 4 cut(s) 17, 166, 370, 402
NlaIV GGNNCC 2 cut(s) 293, 404
NmuCI GTSAC 1 cut(s) 152
NsiI ATGCAT 1 cut(s) 497
PdmI GAANNNNTTC 2 cut(s) 329, 525
PfeI GAWTC 2 cut(s) 254, 550
PflMI CCANNNNNTGG 1 cut(s) 105
PleI GAGTC 2 cut(s) 169, 177
PmaCI CACGTG 1 cut(s) 230
PmlI CACGTG 1 cut(s) 230
PpsI GAGTC 2 cut(s) 169, 177
Ppu21I YACGTR 1 cut(s) 230
Psp6I CCWGG 1 cut(s) 554
PspCI CACGTG 1 cut(s) 230
PspGI CCWGG 1 cut(s) 554
PspN4I GGNNCC 2 cut(s) 293, 404
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 2 cut(s) 285, 305
Sau3AI GATC 1 cut(s) 234
SchI GAGTC 2 cut(s) 169, 177
ScrFI CCNGG 1 cut(s) 556
SetI ASST 7 cut(s) 76, 232, 398, 408, 448, 474, 534
SfaNI GCATC 4 cut(s) 213, 273, 482, 504
SfcI CTRYAG 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 161
Sse9I AATT 6 cut(s) 134, 197, 282, 521, 537, 571
SsiI CCGC 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 554
TaaI ACNGT 1 cut(s) 418
TaiI ACGT 1 cut(s) 232
TasI AATT 6 cut(s) 134, 197, 282, 521, 537, 571
TfiI GAWTC 2 cut(s) 254, 550
Tru1I TTAA 2 cut(s) 285, 305
Tru9I TTAA 2 cut(s) 285, 305
TscAI CASTG 1 cut(s) 505
TseFI GTSAC 1 cut(s) 152
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 2 cut(s) 262, 477
TspRI CASTG 1 cut(s) 505
Van91I CCANNNNNTGG 1 cut(s) 105
XapI RAATTY 2 cut(s) 197, 537
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 2 cut(s) 329, 525
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.