Rh4AG332100

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0016650
Physical Location & Seq
Reverse (-)
64217939 .. 64218975
1037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG332100.1

Sequence Viewer

Length: 579 bp
ATGTCTATGCATAGGCCTCTGTATGCCATTCTTGGAGGAGTGGCTGACATACTGCTATGGAAGGATAAGAAATCAGCTGCTGCAATCTTATTAGGGTCCACCGCATTATGGTTTTTTCTACAAGTACTAGAATACCATTTTGTATCTCTTCTTTGTGACACCCTCATGCTGTTCACGGCTGTTGGAGTCCTCTGGTGCAAATTCGCGGGACGAATATTCAATCGGATGCCGTCGGAGATCAACATTCAACTGCCACAATCCACTTGCATGTACTTCTTTGGAACAGTTAACTGGTTCCTACTGAAGTTCTGTGAGATTACATCCGGAAAAAATTTCAAAATCTTCTTTGTGGCAATGGCTGGTCTATACATGTTGTCATCTATAGGAACTTATATCAGCTCATTGAACCTATTATATACAATCATTCTCTCGGTAGGAACATTGCCAGCTTTGTATGCGCGATATGAAACTCAAGTGGATGCAATCGCGGCCAGATGCATTGGAGTTGTGAAGAATTTATGCAAATTGTTTGAGGTCAAAGTTCTTGACAAGATACCGAGACACAGGAAACTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

192

Amino Acids

21.74

Weight (kDa)

9.37

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 14 - 164 1e-34 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68230 AT1G68230
fragaria_vesca FvH4_4g26750
malus_domestica MD16G1037300.v1.1
prunus_persica Prupe.1G316500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0434431
rosa_laevigata RLG00000006672
rosa_multiflora Rmu_co8483911.1_g000001
rosa_roxburghii Rroxscaffold_5G00375790
rosa_samantha Rh4AG332100 Rh4BG340000 Rh4CG355200 Rh4DG335300
rosa_wichuraiana Rw4G028800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 206, 460, 488
AccIII TCCGGA 1 cut(s) 323
AciI CCGC 3 cut(s) 102, 206, 488
AcoI YGGCCR 1 cut(s) 489
AcsI RAATTY 3 cut(s) 200, 331, 514
AcuI CTGAAG 1 cut(s) 323
AfaI GTAC 2 cut(s) 126, 272
AfiI CCNNNNNNNGG 1 cut(s) 108
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 4 cut(s) 220, 248, 337, 406
AluBI AGCT 3 cut(s) 77, 399, 449
AluI AGCT 3 cut(s) 77, 399, 449
Alw26I GTCTC 1 cut(s) 553
AlwNI CAGNNNCTG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 323
AoxI GGCC 2 cut(s) 14, 489
ApeKI GCWGC 2 cut(s) 77, 80
ApoI RAATTY 3 cut(s) 200, 331, 514
AspLEI GCGC 1 cut(s) 460
AspS9I GGNCC 1 cut(s) 96
AvaII GGWCC 1 cut(s) 96
BbvI GCAGC 2 cut(s) 64, 67
BceAI ACGGC 2 cut(s) 192, 214
BcoDI GTCTC 1 cut(s) 553
BfaI CTAG 2 cut(s) 128, 577
BfmI CTRYAG 1 cut(s) 381
BisI GCNGC 3 cut(s) 78, 81, 489
BlsI GCNGC 3 cut(s) 79, 82, 490
BmcAI AGTACT 1 cut(s) 126
Bme18I GGWCC 1 cut(s) 96
BmgT120I GGNCC 1 cut(s) 96
BmiI GGNNCC 2 cut(s) 97, 296
BmsI GCATC 3 cut(s) 216, 469, 485
BpuEI CTTGAG 1 cut(s) 456
BsaWI WCCGGW 1 cut(s) 323
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 2 cut(s) 360, 440
BseAI TCCGGA 1 cut(s) 323
BseGI GGATG 3 cut(s) 231, 320, 484
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMI GCAATG 2 cut(s) 360, 440
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 2 cut(s) 64, 67
Bsh1236I CGCG 3 cut(s) 206, 460, 488
BshFI GGCC 2 cut(s) 16, 491
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 108
BsmAI GTCTC 1 cut(s) 553
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 2 cut(s) 16, 491
Bsp13I TCCGGA 1 cut(s) 323
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 3 cut(s) 102, 206, 488
BspANI GGCC 2 cut(s) 16, 491
BspEI TCCGGA 1 cut(s) 323
BspFNI CGCG 3 cut(s) 206, 460, 488
BspLI GGNNCC 2 cut(s) 97, 296
BsrDI GCAATG 2 cut(s) 360, 440
BsrI ACTGG 1 cut(s) 296
BssMI GATC 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 447
BstF5I GGATG 3 cut(s) 231, 320, 484
BstFNI CGCG 3 cut(s) 206, 460, 488
BstHHI GCGC 1 cut(s) 460
BstKTI GATC 1 cut(s) 240
BstMAI GTCTC 1 cut(s) 553
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 2 cut(s) 455, 488
BstNSI RCATGY 2 cut(s) 271, 373
BstSFI CTRYAG 1 cut(s) 381
BstUI CGCG 3 cut(s) 206, 460, 488
BstV1I GCAGC 2 cut(s) 64, 67
BsuRI GGCC 2 cut(s) 16, 491
BtsCI GGATG 3 cut(s) 231, 320, 484
Cac8I GCNNGC 1 cut(s) 447
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 96
Csp6I GTAC 2 cut(s) 125, 271
CviAII CATG 3 cut(s) 166, 268, 370
CviJI RGCY 8 cut(s) 16, 44, 77, 179, 359, 399, 449, 491
CviKI_1 RGCY 8 cut(s) 16, 44, 77, 179, 359, 399, 449, 491
CviQI GTAC 2 cut(s) 125, 271
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
EaeI YGGCCR 1 cut(s) 489
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 323
EcoT22I ATGCAT 2 cut(s) 12, 500
FaeI CATG 3 cut(s) 169, 271, 373
FaqI GGGAC 1 cut(s) 222
FatI CATG 3 cut(s) 165, 267, 369
FauI CCCGC 1 cut(s) 199
Fnu4HI GCNGC 3 cut(s) 78, 81, 489
FokI GGATG 3 cut(s) 238, 307, 491
Fsp4HI GCNGC 3 cut(s) 78, 81, 489
FspBI CTAG 2 cut(s) 128, 577
GlaI GCGC 1 cut(s) 459
GluI GCNGC 3 cut(s) 78, 81, 489
HaeIII GGCC 2 cut(s) 16, 491
HapII CCGG 1 cut(s) 324
HhaI GCGC 1 cut(s) 460
Hin1II CATG 3 cut(s) 169, 271, 373
Hin6I GCGC 1 cut(s) 458
HinP1I GCGC 1 cut(s) 458
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 1 cut(s) 186
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 1 cut(s) 324
Hpy166II GTNNAC 3 cut(s) 99, 174, 289
Hpy188I TCNGA 2 cut(s) 225, 235
Hpy188III TCNNGA 2 cut(s) 324, 545
Hpy8I GTNNAC 3 cut(s) 99, 174, 289
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 7 cut(s) 10, 83, 198, 267, 482, 498, 522
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 488
Hsp92II CATG 3 cut(s) 169, 271, 373
HspAI GCGC 1 cut(s) 458
Kpn2I TCCGGA 1 cut(s) 323
KspAI GTTAAC 1 cut(s) 289
Kzo9I GATC 1 cut(s) 237
LpnPI CCDG 7 cut(s) 178, 277, 337, 345, 459, 505, 550
Lsp1109I GCAGC 2 cut(s) 64, 67
LweI GCATC 3 cut(s) 216, 469, 485
MaeI CTAG 2 cut(s) 128, 577
MaeIII GTNAC 1 cut(s) 155
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 3 cut(s) 140, 334, 523
MluCI AATT 4 cut(s) 200, 331, 514, 524
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 2 cut(s) 163, 213
MnlI CCTC 5 cut(s) 27, 29, 173, 200, 526
Mph1103I ATGCAT 2 cut(s) 12, 500
MroI TCCGGA 1 cut(s) 323
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 2 cut(s) 164, 266
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 1 cut(s) 324
MvnI CGCG 3 cut(s) 206, 460, 488
MwoI GCNNNNNNNGC 2 cut(s) 455, 488
NdeII GATC 1 cut(s) 237
NlaIII CATG 3 cut(s) 169, 271, 373
NlaIV GGNNCC 2 cut(s) 97, 296
NmuCI GTSAC 1 cut(s) 155
NsiI ATGCAT 2 cut(s) 12, 500
NspI RCATGY 2 cut(s) 271, 373
PceI AGGCCT 1 cut(s) 16
PciI ACATGT 1 cut(s) 369
PkrI GCNGC 3 cut(s) 79, 82, 490
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PscI ACATGT 1 cut(s) 369
PspN4I GGNNCC 2 cut(s) 97, 296
PspPI GGNCC 1 cut(s) 96
PstNI CAGNNNCTG 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 2 cut(s) 126, 272
RsaNI GTAC 2 cut(s) 125, 271
RseI CAYNNNNRTG 2 cut(s) 164, 266
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 3 cut(s) 78, 81, 489
Sau3AI GATC 1 cut(s) 237
Sau96I GGNCC 1 cut(s) 96
ScaI AGTACT 1 cut(s) 126
SchI GAGTC 1 cut(s) 195
SetI ASST 5 cut(s) 79, 401, 411, 451, 537
SfaNI GCATC 3 cut(s) 216, 469, 485
SfcI CTRYAG 1 cut(s) 381
SinI GGWCC 1 cut(s) 96
SmiMI CAYNNNNRTG 2 cut(s) 164, 266
SmlI CTYRAG 1 cut(s) 471
SmoI CTYRAG 1 cut(s) 471
Sse9I AATT 4 cut(s) 200, 331, 514, 524
SseBI AGGCCT 1 cut(s) 16
SsiI CCGC 3 cut(s) 102, 206, 488
SspI AATATT 1 cut(s) 216
SspMI CTAG 2 cut(s) 128, 577
StuI AGGCCT 1 cut(s) 16
TaaI ACNGT 1 cut(s) 286
TasI AATT 4 cut(s) 200, 331, 514, 524
TatI WGTACW 2 cut(s) 124, 270
TauI GCSGC 1 cut(s) 491
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 2 cut(s) 77, 80
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 1 cut(s) 480
VpaK11BI GGWCC 1 cut(s) 96
XapI RAATTY 3 cut(s) 200, 331, 514
XceI RCATGY 2 cut(s) 271, 373
XspI CTAG 2 cut(s) 128, 577
ZrmI AGTACT 1 cut(s) 126
Zsp2I ATGCAT 2 cut(s) 12, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.