Rw4G028800

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016650
Physical Location & Seq
Reverse (-)
55413515 .. 55414520
1006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G028800.1

Sequence Viewer

Length: 585 bp
ATGTCTATGCATGGGCCTCTGTATGACATTCTTGGAGGAGGTATAGTGGCTGACATACTGCTATGGAAGGATAAGAAATCAGCTGCTGCAATCTTATTAGGGTTCACCGCATTATGGTTTTTTCTACAAGTACTAGAATACCATTTTGTATCTCTTCTTTGTGACACCCTCATGCTGTTCATGGCTGTTGGAGTCCTCTGGTGCAAATTCGCGGGACGAATATTCAATCGGATGCCGTCGGAGATCAACATTCAACTGCCACAATCTACTTGCATATACTTCTTTGGAACAGTTAACTGGTTCCTACTGAAGTTCTGTGAAATTACATCCGGAAAAAATTTCAAACTCTTCTTTGTGGCAATGGCTGGTCTATACATGTTGTCATCTATAGGAACTTATTTCAGCTCATTGAACCTATTATATACAACCATTCTCTCGGTAGGAACATTGCCAGCTTTGTATGCGCGATATGAAACTCAAGTGGATGCAATCGCGGCCAGATGCATTGGAGTTGTGAAGAATTTATGCAAATTGTTTGAGGTCAAAGTTCTTGACAAGATACCGAGGCACAGGAAACTTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

194

Amino Acids

21.95

Weight (kDa)

9.15

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 16 - 166 1.1e-35 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68230 AT1G68230
fragaria_vesca FvH4_4g26750
malus_domestica MD16G1037300.v1.1
prunus_persica Prupe.1G316500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0434431
rosa_laevigata RLG00000006672
rosa_multiflora Rmu_co8483911.1_g000001
rosa_roxburghii Rroxscaffold_5G00375790
rosa_samantha Rh4AG332100 Rh4BG340000 Rh4CG355200 Rh4DG335300
rosa_wichuraiana Rw4G028800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 212, 466, 494
AccIII TCCGGA 1 cut(s) 329
AciI CCGC 3 cut(s) 108, 212, 494
AcoI YGGCCR 1 cut(s) 495
AcsI RAATTY 3 cut(s) 206, 337, 520
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 114
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 4 cut(s) 226, 254, 343, 412
AluBI AGCT 3 cut(s) 83, 405, 455
AluI AGCT 3 cut(s) 83, 405, 455
AlwNI CAGNNNCTG 1 cut(s) 86
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 2 cut(s) 14, 495
ApeKI GCWGC 2 cut(s) 83, 86
ApoI RAATTY 3 cut(s) 206, 337, 520
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 1 cut(s) 14
AsuHPI GGTGA 1 cut(s) 97
BbvI GCAGC 2 cut(s) 70, 73
BceAI ACGGC 1 cut(s) 220
BfaI CTAG 2 cut(s) 134, 583
BfmI CTRYAG 1 cut(s) 387
BisI GCNGC 3 cut(s) 84, 87, 495
BlsI GCNGC 3 cut(s) 85, 88, 496
BmcAI AGTACT 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 14
BmiI GGNNCC 1 cut(s) 302
BmsI GCATC 3 cut(s) 222, 475, 491
BpuEI CTTGAG 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 563
BsaWI WCCGGW 1 cut(s) 329
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 302
Bse3DI GCAATG 2 cut(s) 366, 446
BseAI TCCGGA 1 cut(s) 329
BseDI CCNNGG 1 cut(s) 563
BseGI GGATG 3 cut(s) 237, 326, 490
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMI GCAATG 2 cut(s) 366, 446
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 2 cut(s) 70, 73
Bsh1236I CGCG 3 cut(s) 212, 466, 494
BshFI GGCC 2 cut(s) 16, 497
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 1 cut(s) 228
BslI CCNNNNNNNGG 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 228
BsnI GGCC 2 cut(s) 16, 497
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 1 cut(s) 243
BspACI CCGC 3 cut(s) 108, 212, 494
BspANI GGCC 2 cut(s) 16, 497
BspEI TCCGGA 1 cut(s) 329
BspFNI CGCG 3 cut(s) 212, 466, 494
BspLI GGNNCC 1 cut(s) 302
BsrDI GCAATG 2 cut(s) 366, 446
BsrI ACTGG 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 563
BssMI GATC 1 cut(s) 243
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 2 cut(s) 159, 353
BstC8I GCNNGC 1 cut(s) 453
BstF5I GGATG 3 cut(s) 237, 326, 490
BstFNI CGCG 3 cut(s) 212, 466, 494
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 1 cut(s) 246
BstMBI GATC 1 cut(s) 243
BstMWI GCNNNNNNNGC 2 cut(s) 461, 494
BstNSI RCATGY 1 cut(s) 379
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 3 cut(s) 212, 466, 494
BstV1I GCAGC 2 cut(s) 70, 73
BsuRI GGCC 2 cut(s) 16, 497
BtsCI GGATG 3 cut(s) 237, 326, 490
Cac8I GCNNGC 1 cut(s) 453
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 131
CviAII CATG 4 cut(s) 11, 172, 181, 376
CviJI RGCY 8 cut(s) 16, 50, 83, 185, 365, 405, 455, 497
CviKI_1 RGCY 8 cut(s) 16, 50, 83, 185, 365, 405, 455, 497
CviQI GTAC 1 cut(s) 131
DpnI GATC 1 cut(s) 245
DpnII GATC 1 cut(s) 243
EaeI YGGCCR 1 cut(s) 495
Eam1104I CTCTTC 2 cut(s) 159, 353
EarI CTCTTC 2 cut(s) 159, 353
Eco57I CTGAAG 1 cut(s) 329
EcoT22I ATGCAT 2 cut(s) 12, 506
FaeI CATG 4 cut(s) 14, 175, 184, 379
FaqI GGGAC 1 cut(s) 228
FatI CATG 4 cut(s) 10, 171, 180, 375
FauI CCCGC 1 cut(s) 205
Fnu4HI GCNGC 3 cut(s) 84, 87, 495
FokI GGATG 3 cut(s) 244, 313, 497
Fsp4HI GCNGC 3 cut(s) 84, 87, 495
FspBI CTAG 2 cut(s) 134, 583
GlaI GCGC 1 cut(s) 465
GluI GCNGC 3 cut(s) 84, 87, 495
HaeIII GGCC 2 cut(s) 16, 497
HapII CCGG 1 cut(s) 330
HhaI GCGC 1 cut(s) 466
Hin1II CATG 4 cut(s) 14, 175, 184, 379
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 1 cut(s) 192
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 105, 295
Hpy188I TCNGA 2 cut(s) 231, 241
Hpy188III TCNNGA 2 cut(s) 330, 551
Hpy8I GTNNAC 2 cut(s) 105, 295
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4V TGCA 7 cut(s) 10, 89, 204, 273, 488, 504, 528
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 494
Hsp92II CATG 4 cut(s) 14, 175, 184, 379
HspAI GCGC 1 cut(s) 464
Kpn2I TCCGGA 1 cut(s) 329
KspAI GTTAAC 1 cut(s) 295
Kzo9I GATC 1 cut(s) 243
LpnPI CCDG 7 cut(s) 184, 283, 343, 351, 465, 511, 556
Lsp1109I GCAGC 2 cut(s) 70, 73
LweI GCATC 3 cut(s) 222, 475, 491
MaeI CTAG 2 cut(s) 134, 583
MaeIII GTNAC 1 cut(s) 161
MalI GATC 1 cut(s) 245
MboI GATC 1 cut(s) 243
MboII GAAGA 3 cut(s) 146, 340, 529
MluCI AATT 5 cut(s) 206, 321, 337, 520, 530
MlyI GAGTC 1 cut(s) 201
MmeI TCCRAC 2 cut(s) 169, 219
MnlI CCTC 7 cut(s) 27, 29, 32, 179, 206, 532, 558
Mph1103I ATGCAT 2 cut(s) 12, 506
MroI TCCGGA 1 cut(s) 329
MseI TTAA 2 cut(s) 294, 579
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 330
MvnI CGCG 3 cut(s) 212, 466, 494
MwoI GCNNNNNNNGC 2 cut(s) 461, 494
NdeII GATC 1 cut(s) 243
NlaIII CATG 4 cut(s) 14, 175, 184, 379
NlaIV GGNNCC 1 cut(s) 302
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 2 cut(s) 12, 506
NspI RCATGY 1 cut(s) 379
PciI ACATGT 1 cut(s) 375
PkrI GCNGC 3 cut(s) 85, 88, 496
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PscI ACATGT 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 302
PspPI GGNCC 1 cut(s) 14
PstNI CAGNNNCTG 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 170
SaqAI TTAA 2 cut(s) 294, 579
SatI GCNGC 3 cut(s) 84, 87, 495
Sau3AI GATC 1 cut(s) 243
Sau96I GGNCC 1 cut(s) 14
ScaI AGTACT 1 cut(s) 132
SchI GAGTC 1 cut(s) 201
SetI ASST 6 cut(s) 43, 85, 407, 417, 457, 543
SfaNI GCATC 3 cut(s) 222, 475, 491
SfcI CTRYAG 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
Sse9I AATT 5 cut(s) 206, 321, 337, 520, 530
SsiI CCGC 3 cut(s) 108, 212, 494
SspI AATATT 1 cut(s) 222
SspMI CTAG 2 cut(s) 134, 583
TaaI ACNGT 1 cut(s) 292
TasI AATT 5 cut(s) 206, 321, 337, 520, 530
TatI WGTACW 1 cut(s) 130
TauI GCSGC 1 cut(s) 497
Tru1I TTAA 2 cut(s) 294, 579
Tru9I TTAA 2 cut(s) 294, 579
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 2 cut(s) 83, 86
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 2 cut(s) 169, 486
XapI RAATTY 3 cut(s) 206, 337, 520
XceI RCATGY 1 cut(s) 379
XspI CTAG 2 cut(s) 134, 583
ZrmI AGTACT 1 cut(s) 132
Zsp2I ATGCAT 2 cut(s) 12, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.