Rh4BG340000

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0016650
Physical Location & Seq
Reverse (-)
50205015 .. 50207727
2713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG340000.1

Sequence Viewer

Length: 465 bp
ATGTCTATGCATAGGCCTCTGTATGCCATTCTTGGAGGAGGAATAGTGGCTGACATACTGCTATGGAAGGATAAGAAATCAGCTGCTGCAATCTTATTAGGGTTCACCGCATTATGGTTTTTTCTACAAGTACTAGAATACCATTTTGTATCTCTTCTTTGTGACACCCTCATGCTGTTCATGGCTATTGGAGTCCTCTGGTGCAAATTCGCGGGACGAATATTCAATCGGATGCCGTCGGAGATCAACATTCAACTGCCACAATCTACTTGCATATACATCTTTGGAACAGTTAACTGGTTCCTACTGAAGTTCTGTGAAATTACATCCGGAAAAAATTTCAAACTCTTCTTTGTGGCAATGGCTGGTCTATACATGTTGTCATCTATAGGAACTTATATCAGCTCATTGAACCTATTATATACAATAATGGCACTGAGATCTCGCAAGGGAGGCCTTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

154

Amino Acids

17.45

Weight (kDa)

9.3

Isoelectric Point (pI)

52.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 16 - 144 1.4e-26 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68230 AT1G68230
fragaria_vesca FvH4_4g26750
malus_domestica MD16G1037300.v1.1
prunus_persica Prupe.1G316500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0434431
rosa_laevigata RLG00000006672
rosa_multiflora Rmu_co8483911.1_g000001
rosa_roxburghii Rroxscaffold_5G00375790
rosa_samantha Rh4AG332100 Rh4BG340000 Rh4CG355200 Rh4DG335300
rosa_wichuraiana Rw4G028800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 212
AccIII TCCGGA 1 cut(s) 329
AciI CCGC 2 cut(s) 108, 212
AcsI RAATTY 2 cut(s) 206, 337
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 114
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 4 cut(s) 226, 254, 343, 412
AluBI AGCT 2 cut(s) 83, 405
AluI AGCT 2 cut(s) 83, 405
AlwNI CAGNNNCTG 1 cut(s) 86
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 2 cut(s) 14, 454
ApeKI GCWGC 2 cut(s) 83, 86
ApoI RAATTY 2 cut(s) 206, 337
AsuHPI GGTGA 1 cut(s) 97
BbvI GCAGC 2 cut(s) 70, 73
BceAI ACGGC 1 cut(s) 220
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 387
BglII AGATCT 1 cut(s) 440
BisI GCNGC 2 cut(s) 84, 87
BlsI GCNGC 2 cut(s) 85, 88
BmcAI AGTACT 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 302
BmsI GCATC 1 cut(s) 222
BsaWI WCCGGW 1 cut(s) 329
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse1I ACTGG 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 366
BseAI TCCGGA 1 cut(s) 329
BseGI GGATG 2 cut(s) 237, 326
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 1 cut(s) 428
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 2 cut(s) 70, 73
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 2 cut(s) 16, 456
BsiSI CCGG 1 cut(s) 330
BslFI GGGAC 1 cut(s) 228
BslI CCNNNNNNNGG 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 228
BsnI GGCC 2 cut(s) 16, 456
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 2 cut(s) 243, 440
BspACI CCGC 2 cut(s) 108, 212
BspANI GGCC 2 cut(s) 16, 456
BspCNI CTCAG 1 cut(s) 429
BspEI TCCGGA 1 cut(s) 329
BspFNI CGCG 1 cut(s) 212
BspLI GGNNCC 1 cut(s) 302
BsrDI GCAATG 1 cut(s) 366
BsrI ACTGG 1 cut(s) 302
BssMI GATC 2 cut(s) 243, 440
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 2 cut(s) 159, 353
BstDEI CTNAG 1 cut(s) 437
BstF5I GGATG 2 cut(s) 237, 326
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 2 cut(s) 246, 443
BstMBI GATC 2 cut(s) 243, 440
BstMWI GCNNNNNNNGC 1 cut(s) 453
BstNSI RCATGY 1 cut(s) 379
BstSFI CTRYAG 1 cut(s) 387
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 70, 73
BstX2I RGATCY 1 cut(s) 440
BstYI RGATCY 1 cut(s) 440
BsuRI GGCC 2 cut(s) 16, 456
BtsCI GGATG 2 cut(s) 237, 326
BtsIMutI CAGTG 1 cut(s) 434
CaiI CAGNNNCTG 1 cut(s) 86
Csp6I GTAC 1 cut(s) 131
CviAII CATG 3 cut(s) 172, 181, 376
CviJI RGCY 7 cut(s) 16, 50, 83, 185, 365, 405, 456
CviKI_1 RGCY 7 cut(s) 16, 50, 83, 185, 365, 405, 456
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 1 cut(s) 437
DpnI GATC 2 cut(s) 245, 442
DpnII GATC 2 cut(s) 243, 440
Eam1104I CTCTTC 2 cut(s) 159, 353
EarI CTCTTC 2 cut(s) 159, 353
Eco147I AGGCCT 2 cut(s) 16, 456
Eco57I CTGAAG 1 cut(s) 329
EcoT22I ATGCAT 1 cut(s) 12
FaeI CATG 3 cut(s) 175, 184, 379
FaqI GGGAC 1 cut(s) 228
FatI CATG 3 cut(s) 171, 180, 375
FauI CCCGC 1 cut(s) 205
Fnu4HI GCNGC 2 cut(s) 84, 87
FokI GGATG 2 cut(s) 244, 313
Fsp4HI GCNGC 2 cut(s) 84, 87
FspBI CTAG 1 cut(s) 134
GluI GCNGC 2 cut(s) 84, 87
HaeIII GGCC 2 cut(s) 16, 456
HapII CCGG 1 cut(s) 330
Hin1II CATG 3 cut(s) 175, 184, 379
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 1 cut(s) 192
HpaI GTTAAC 1 cut(s) 295
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 2 cut(s) 105, 295
Hpy188I TCNGA 2 cut(s) 231, 241
Hpy188III TCNNGA 1 cut(s) 330
Hpy8I GTNNAC 2 cut(s) 105, 295
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4V TGCA 4 cut(s) 10, 89, 204, 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 453
HpyF3I CTNAG 1 cut(s) 437
Hsp92II CATG 3 cut(s) 175, 184, 379
Kpn2I TCCGGA 1 cut(s) 329
KspAI GTTAAC 1 cut(s) 295
Kzo9I GATC 2 cut(s) 243, 440
LpnPI CCDG 4 cut(s) 184, 283, 343, 351
Lsp1109I GCAGC 2 cut(s) 70, 73
LweI GCATC 1 cut(s) 222
MaeI CTAG 1 cut(s) 134
MaeIII GTNAC 1 cut(s) 161
MalI GATC 2 cut(s) 245, 442
MboI GATC 2 cut(s) 243, 440
MboII GAAGA 2 cut(s) 146, 340
MflI RGATCY 1 cut(s) 440
MluCI AATT 3 cut(s) 206, 321, 337
MlyI GAGTC 1 cut(s) 201
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 6 cut(s) 27, 29, 32, 179, 206, 446
Mph1103I ATGCAT 1 cut(s) 12
MroI TCCGGA 1 cut(s) 329
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 170
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 453
NdeII GATC 2 cut(s) 243, 440
NlaIII CATG 3 cut(s) 175, 184, 379
NlaIV GGNNCC 1 cut(s) 302
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 12
NspI RCATGY 1 cut(s) 379
PceI AGGCCT 2 cut(s) 16, 456
PciI ACATGT 1 cut(s) 375
PkrI GCNGC 2 cut(s) 85, 88
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PscI ACATGT 1 cut(s) 375
PspN4I GGNNCC 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 440
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 170
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 2 cut(s) 84, 87
Sau3AI GATC 2 cut(s) 243, 440
ScaI AGTACT 1 cut(s) 132
SchI GAGTC 1 cut(s) 201
SetI ASST 3 cut(s) 85, 407, 417
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 3 cut(s) 206, 321, 337
SseBI AGGCCT 2 cut(s) 16, 456
SsiI CCGC 2 cut(s) 108, 212
SspI AATATT 1 cut(s) 222
SspMI CTAG 1 cut(s) 134
StuI AGGCCT 2 cut(s) 16, 456
TaaI ACNGT 1 cut(s) 292
TasI AATT 3 cut(s) 206, 321, 337
TatI WGTACW 1 cut(s) 130
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 2 cut(s) 83, 86
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 1 cut(s) 169
TspRI CASTG 1 cut(s) 441
XapI RAATTY 2 cut(s) 206, 337
XceI RCATGY 1 cut(s) 379
XspI CTAG 1 cut(s) 134
ZrmI AGTACT 1 cut(s) 132
Zsp2I ATGCAT 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.