Rroxscaffold_5G00375790

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0016650
Physical Location & Seq
Reverse (-)
56532795 .. 56533822
1028 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375790.1

Sequence Viewer

Length: 564 bp
ATGCATGGGCCTCTGTATGCCATTCTTGGAGGAGGTATAGTGGCTGACATACTGCTATGGAAGGATAAGAAATCAGCTGCTGCAATCTTATTAGGGTTCACCGCATTATGGTTTTTTCTACAAGTACTAGAATACCATTTTGTATCTCTTCTTTGTGACACCCTCATGCTGTTCATGGCTGTTGGAGTCCTCTGGTGCAAATTCGTGGGACGAATATTCAATCGGATGCCGTCGGAGATCAACATTCAACTGCCACAATCTACTTGCATATACTTCTTTGGAACAGTTAACTGGTTCCTACTGAAGTTCTGTGAAATTACATCCGGAAAAAATTTCAAACTCTTCTTTGTGGCAATGGCTGGTCTATACATGTTGTCATCTATAGGAACTTATATCAGCTCATTGAACCTATTATATACAAGAACATTGCCAGCTTTGTATGCGCGATATGAAACTCAGGTGGATGCAATCGCGGCCAGATGCATTGGAGTTGTGAAGAATTTATGCAAATTGTTTGAGGTCAAAGTTCTTGACAAGATACCGAGGCACAGGAAACTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

187

Amino Acids

21.27

Weight (kDa)

9.37

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 14 - 159 3.8e-31 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68230 AT1G68230
fragaria_vesca FvH4_4g26750
malus_domestica MD16G1037300.v1.1
prunus_persica Prupe.1G316500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0434431
rosa_laevigata RLG00000006672
rosa_multiflora Rmu_co8483911.1_g000001
rosa_roxburghii Rroxscaffold_5G00375790
rosa_samantha Rh4AG332100 Rh4BG340000 Rh4CG355200 Rh4DG335300
rosa_wichuraiana Rw4G028800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 445, 473
AccIII TCCGGA 1 cut(s) 323
AciI CCGC 2 cut(s) 102, 473
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 3 cut(s) 200, 331, 499
AcuI CTGAAG 1 cut(s) 323
AfaI GTAC 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 108
AflIII ACRYGT 1 cut(s) 369
AgsI TTSAA 4 cut(s) 220, 248, 337, 406
AluBI AGCT 3 cut(s) 77, 399, 434
AluI AGCT 3 cut(s) 77, 399, 434
AlwNI CAGNNNCTG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 323
AoxI GGCC 2 cut(s) 8, 474
ApeKI GCWGC 2 cut(s) 77, 80
ApoI RAATTY 3 cut(s) 200, 331, 499
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 1 cut(s) 8
AsuHPI GGTGA 1 cut(s) 91
BbvI GCAGC 2 cut(s) 64, 67
BceAI ACGGC 1 cut(s) 214
BfaI CTAG 2 cut(s) 128, 562
BfmI CTRYAG 1 cut(s) 381
BisI GCNGC 3 cut(s) 78, 81, 474
BlsI GCNGC 3 cut(s) 79, 82, 475
BmcAI AGTACT 1 cut(s) 126
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 1 cut(s) 296
BmsI GCATC 3 cut(s) 216, 454, 470
BsaJI CCNNGG 1 cut(s) 542
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 2 cut(s) 24, 54
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 2 cut(s) 360, 425
BseAI TCCGGA 1 cut(s) 323
BseDI CCNNGG 1 cut(s) 542
BseGI GGATG 3 cut(s) 231, 320, 469
BseLI CCNNNNNNNGG 1 cut(s) 108
BseMI GCAATG 2 cut(s) 360, 425
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 1 cut(s) 296
BseRI GAGGAG 1 cut(s) 45
BseXI GCAGC 2 cut(s) 64, 67
Bsh1236I CGCG 2 cut(s) 445, 473
BshFI GGCC 2 cut(s) 10, 476
BsiSI CCGG 1 cut(s) 324
BslFI GGGAC 1 cut(s) 222
BslI CCNNNNNNNGG 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 222
BsnI GGCC 2 cut(s) 10, 476
Bsp13I TCCGGA 1 cut(s) 323
Bsp143I GATC 1 cut(s) 237
BspACI CCGC 2 cut(s) 102, 473
BspANI GGCC 2 cut(s) 10, 476
BspCNI CTCAG 1 cut(s) 469
BspEI TCCGGA 1 cut(s) 323
BspFNI CGCG 2 cut(s) 445, 473
BspLI GGNNCC 1 cut(s) 296
BsrDI GCAATG 2 cut(s) 360, 425
BsrI ACTGG 1 cut(s) 296
BssECI CCNNGG 1 cut(s) 542
BssMI GATC 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 2 cut(s) 153, 347
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 456
BstF5I GGATG 3 cut(s) 231, 320, 469
BstFNI CGCG 2 cut(s) 445, 473
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 1 cut(s) 240
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 2 cut(s) 440, 473
BstNSI RCATGY 1 cut(s) 373
BstSFI CTRYAG 1 cut(s) 381
BstUI CGCG 2 cut(s) 445, 473
BstV1I GCAGC 2 cut(s) 64, 67
BsuRI GGCC 2 cut(s) 10, 476
BtsCI GGATG 3 cut(s) 231, 320, 469
Cac8I GCNNGC 1 cut(s) 432
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 1 cut(s) 8
Csp6I GTAC 1 cut(s) 125
CviAII CATG 4 cut(s) 5, 166, 175, 370
CviJI RGCY 8 cut(s) 10, 44, 77, 179, 359, 399, 434, 476
CviKI_1 RGCY 8 cut(s) 10, 44, 77, 179, 359, 399, 434, 476
CviQI GTAC 1 cut(s) 125
DdeI CTNAG 1 cut(s) 456
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
EaeI YGGCCR 1 cut(s) 474
Eam1104I CTCTTC 2 cut(s) 153, 347
EarI CTCTTC 2 cut(s) 153, 347
Eco57I CTGAAG 1 cut(s) 323
EcoT22I ATGCAT 2 cut(s) 6, 485
FaeI CATG 4 cut(s) 8, 169, 178, 373
FaqI GGGAC 1 cut(s) 222
FatI CATG 4 cut(s) 4, 165, 174, 369
Fnu4HI GCNGC 3 cut(s) 78, 81, 474
FokI GGATG 3 cut(s) 238, 307, 476
Fsp4HI GCNGC 3 cut(s) 78, 81, 474
FspBI CTAG 2 cut(s) 128, 562
GlaI GCGC 1 cut(s) 444
GluI GCNGC 3 cut(s) 78, 81, 474
HaeIII GGCC 2 cut(s) 10, 476
HapII CCGG 1 cut(s) 324
HhaI GCGC 1 cut(s) 445
Hin1II CATG 4 cut(s) 8, 169, 178, 373
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 1 cut(s) 186
HpaI GTTAAC 1 cut(s) 289
HpaII CCGG 1 cut(s) 324
HphI GGTGA 1 cut(s) 91
Hpy166II GTNNAC 2 cut(s) 99, 289
Hpy188I TCNGA 2 cut(s) 225, 235
Hpy188III TCNNGA 2 cut(s) 324, 530
Hpy8I GTNNAC 2 cut(s) 99, 289
Hpy99I CGWCG 1 cut(s) 235
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 7 cut(s) 4, 83, 198, 267, 467, 483, 507
HpyF10VI GCNNNNNNNGC 2 cut(s) 440, 473
HpyF3I CTNAG 1 cut(s) 456
Hsp92II CATG 4 cut(s) 8, 169, 178, 373
HspAI GCGC 1 cut(s) 443
Kpn2I TCCGGA 1 cut(s) 323
KspAI GTTAAC 1 cut(s) 289
Kzo9I GATC 1 cut(s) 237
LpnPI CCDG 8 cut(s) 178, 277, 337, 345, 443, 444, 490, 535
Lsp1109I GCAGC 2 cut(s) 64, 67
LweI GCATC 3 cut(s) 216, 454, 470
MaeI CTAG 2 cut(s) 128, 562
MaeIII GTNAC 1 cut(s) 155
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 3 cut(s) 140, 334, 508
MluCI AATT 5 cut(s) 200, 315, 331, 499, 509
MlyI GAGTC 1 cut(s) 195
MmeI TCCRAC 2 cut(s) 163, 213
MnlI CCTC 7 cut(s) 21, 23, 26, 173, 200, 511, 537
Mph1103I ATGCAT 2 cut(s) 6, 485
MroI TCCGGA 1 cut(s) 323
MseI TTAA 1 cut(s) 288
MslI CAYNNNNRTG 1 cut(s) 164
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 1 cut(s) 324
MvnI CGCG 2 cut(s) 445, 473
MwoI GCNNNNNNNGC 2 cut(s) 440, 473
NdeII GATC 1 cut(s) 237
NlaIII CATG 4 cut(s) 8, 169, 178, 373
NlaIV GGNNCC 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 155
NsiI ATGCAT 2 cut(s) 6, 485
NspI RCATGY 1 cut(s) 373
PciI ACATGT 1 cut(s) 369
PkrI GCNGC 3 cut(s) 79, 82, 475
PleI GAGTC 1 cut(s) 194
PpsI GAGTC 1 cut(s) 194
PscI ACATGT 1 cut(s) 369
PspN4I GGNNCC 1 cut(s) 296
PspPI GGNCC 1 cut(s) 8
PstNI CAGNNNCTG 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 1 cut(s) 126
RsaNI GTAC 1 cut(s) 125
RseI CAYNNNNRTG 1 cut(s) 164
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 3 cut(s) 78, 81, 474
Sau3AI GATC 1 cut(s) 237
Sau96I GGNCC 1 cut(s) 8
ScaI AGTACT 1 cut(s) 126
SchI GAGTC 1 cut(s) 195
SetI ASST 7 cut(s) 37, 79, 401, 411, 436, 462, 522
SfaNI GCATC 3 cut(s) 216, 454, 470
SfcI CTRYAG 1 cut(s) 381
SmiMI CAYNNNNRTG 1 cut(s) 164
Sse9I AATT 5 cut(s) 200, 315, 331, 499, 509
SsiI CCGC 2 cut(s) 102, 473
SspI AATATT 1 cut(s) 216
SspMI CTAG 2 cut(s) 128, 562
TaaI ACNGT 1 cut(s) 286
TasI AATT 5 cut(s) 200, 315, 331, 499, 509
TatI WGTACW 1 cut(s) 124
TauI GCSGC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 2 cut(s) 77, 80
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 2 cut(s) 163, 465
XapI RAATTY 3 cut(s) 200, 331, 499
XceI RCATGY 1 cut(s) 373
XspI CTAG 2 cut(s) 128, 562
ZrmI AGTACT 1 cut(s) 126
Zsp2I ATGCAT 2 cut(s) 6, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.