Rh4CG355200

Reticulon-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0016650
Physical Location & Seq
Reverse (-)
61108211 .. 61109346
1136 bp
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UTR
Exon/CDS
Intron
Rh4CG355200.1

Sequence Viewer

Length: 414 bp
ATGCTGTTCATGGCTATTGGAGTCCTCTGGTGCAAATTCGCGGGACGAATATTCAATCGGATGCCGTCGGAGATCAACATTCAACTGCCACAATCTACTTGCATATACATCTTTGGAACAGTTAACTGGTTCCTACTGAAGTTCTGTGAAATTACATCCGGAAAAAATTTCAAACTCTTCTTTGTGGCAATGGCTGGTCTATACATGTTGTCATCTATAGGAACTTATATCAGCTCATTGAACCTATTATATACAATCATTCTCTCGGTAGGAACATTGCCAGCTTTGTATGCGCGATATGAAACTCAGGTGGATACAATCGCGGCCAGATGCATTGGAGTGGTGAAGAATTTATGCAAATTGTTTCAGGTCAAAGTTCTTGACAAGATACCGAGGCACAGGAAACTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05010 map05010
Pfam Domains
Protein Families
Reticulon

Protein Analysis

137

Amino Acids

15.6

Weight (kDa)

9.68

Isoelectric Point (pI)

27.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 1 - 109 7.3e-19 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016650)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68230 AT1G68230
fragaria_vesca FvH4_4g26750
malus_domestica MD16G1037300.v1.1
prunus_persica Prupe.1G316500_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0434431
rosa_laevigata RLG00000006672
rosa_multiflora Rmu_co8483911.1_g000001
rosa_roxburghii Rroxscaffold_5G00375790
rosa_samantha Rh4AG332100 Rh4BG340000 Rh4CG355200 Rh4DG335300
rosa_wichuraiana Rw4G028800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 41, 295, 323
AccIII TCCGGA 1 cut(s) 158
AciI CCGC 2 cut(s) 41, 323
AcoI YGGCCR 1 cut(s) 324
AcsI RAATTY 3 cut(s) 35, 166, 349
AcuI CTGAAG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 4 cut(s) 55, 83, 172, 241
AluBI AGCT 2 cut(s) 234, 284
AluI AGCT 2 cut(s) 234, 284
Aor13HI TCCGGA 1 cut(s) 158
AoxI GGCC 1 cut(s) 324
ApoI RAATTY 3 cut(s) 35, 166, 349
AspLEI GCGC 1 cut(s) 295
AsuHPI GGTGA 1 cut(s) 355
BceAI ACGGC 1 cut(s) 49
BciVI GTATCC 1 cut(s) 307
BfaI CTAG 1 cut(s) 412
BfmI CTRYAG 1 cut(s) 216
BfuI GTATCC 1 cut(s) 307
BisI GCNGC 1 cut(s) 324
BlsI GCNGC 1 cut(s) 325
BmiI GGNNCC 1 cut(s) 131
BmsI GCATC 2 cut(s) 51, 320
BsaJI CCNNGG 1 cut(s) 392
BsaWI WCCGGW 1 cut(s) 158
Bse1I ACTGG 1 cut(s) 131
Bse3DI GCAATG 2 cut(s) 195, 275
BseAI TCCGGA 1 cut(s) 158
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 2 cut(s) 66, 155
BseMI GCAATG 2 cut(s) 195, 275
BseMII CTCAG 1 cut(s) 320
BseNI ACTGG 1 cut(s) 131
Bsh1236I CGCG 3 cut(s) 41, 295, 323
BshFI GGCC 1 cut(s) 326
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 57
BsnI GGCC 1 cut(s) 326
Bsp13I TCCGGA 1 cut(s) 158
Bsp143I GATC 1 cut(s) 72
BspACI CCGC 2 cut(s) 41, 323
BspANI GGCC 1 cut(s) 326
BspCNI CTCAG 1 cut(s) 319
BspEI TCCGGA 1 cut(s) 158
BspFNI CGCG 3 cut(s) 41, 295, 323
BspLI GGNNCC 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 195, 275
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 1 cut(s) 72
Bst4CI ACNGT 1 cut(s) 121
Bst6I CTCTTC 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 282
BstDEI CTNAG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 66, 155
BstFNI CGCG 3 cut(s) 41, 295, 323
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 1 cut(s) 75
BstMBI GATC 1 cut(s) 72
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstNSI RCATGY 1 cut(s) 208
BstSFI CTRYAG 1 cut(s) 216
BstUI CGCG 3 cut(s) 41, 295, 323
BsuI GTATCC 1 cut(s) 307
BsuRI GGCC 1 cut(s) 326
BtsCI GGATG 2 cut(s) 66, 155
Cac8I GCNNGC 1 cut(s) 282
CfoI GCGC 1 cut(s) 295
CviAII CATG 2 cut(s) 10, 205
CviJI RGCY 5 cut(s) 14, 194, 234, 284, 326
CviKI_1 RGCY 5 cut(s) 14, 194, 234, 284, 326
DdeI CTNAG 1 cut(s) 306
DpnI GATC 1 cut(s) 74
DpnII GATC 1 cut(s) 72
EaeI YGGCCR 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 158
EcoT22I ATGCAT 1 cut(s) 335
FaeI CATG 2 cut(s) 13, 208
FaqI GGGAC 1 cut(s) 57
FatI CATG 2 cut(s) 9, 204
FauI CCCGC 1 cut(s) 34
Fnu4HI GCNGC 1 cut(s) 324
FokI GGATG 2 cut(s) 73, 142
Fsp4HI GCNGC 1 cut(s) 324
FspBI CTAG 1 cut(s) 412
GlaI GCGC 1 cut(s) 294
GluI GCNGC 1 cut(s) 324
HaeIII GGCC 1 cut(s) 326
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 295
Hin1II CATG 2 cut(s) 13, 208
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 1 cut(s) 21
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 1 cut(s) 159
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 60, 70
Hpy188III TCNNGA 2 cut(s) 159, 380
Hpy8I GTNNAC 1 cut(s) 124
Hpy99I CGWCG 1 cut(s) 70
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4V TGCA 4 cut(s) 33, 102, 333, 357
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
HpyF3I CTNAG 1 cut(s) 306
Hsp92II CATG 2 cut(s) 13, 208
HspAI GCGC 1 cut(s) 293
Kpn2I TCCGGA 1 cut(s) 158
KspAI GTTAAC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 72
LpnPI CCDG 9 cut(s) 13, 112, 172, 180, 293, 294, 340, 353, 385
LweI GCATC 2 cut(s) 51, 320
MaeI CTAG 1 cut(s) 412
MalI GATC 1 cut(s) 74
MboI GATC 1 cut(s) 72
MboII GAAGA 2 cut(s) 169, 358
MluCI AATT 5 cut(s) 35, 150, 166, 349, 359
MlyI GAGTC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 2 cut(s) 35, 387
Mph1103I ATGCAT 1 cut(s) 335
MroI TCCGGA 1 cut(s) 158
MseI TTAA 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 338
MspI CCGG 1 cut(s) 159
MvnI CGCG 3 cut(s) 41, 295, 323
MwoI GCNNNNNNNGC 1 cut(s) 290
NdeII GATC 1 cut(s) 72
NlaIII CATG 2 cut(s) 13, 208
NlaIV GGNNCC 1 cut(s) 131
NsiI ATGCAT 1 cut(s) 335
NspI RCATGY 1 cut(s) 208
PciI ACATGT 1 cut(s) 204
PkrI GCNGC 1 cut(s) 325
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
PscI ACATGT 1 cut(s) 204
PspN4I GGNNCC 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 1 cut(s) 324
Sau3AI GATC 1 cut(s) 72
SchI GAGTC 1 cut(s) 30
SetI ASST 5 cut(s) 236, 246, 286, 312, 372
SfaNI GCATC 2 cut(s) 51, 320
SfcI CTRYAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 338
Sse9I AATT 5 cut(s) 35, 150, 166, 349, 359
SsiI CCGC 2 cut(s) 41, 323
SspI AATATT 1 cut(s) 51
SspMI CTAG 1 cut(s) 412
TaaI ACNGT 1 cut(s) 121
TasI AATT 5 cut(s) 35, 150, 166, 349, 359
TauI GCSGC 1 cut(s) 326
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TspDTI ATGAA 1 cut(s) 315
XapI RAATTY 3 cut(s) 35, 166, 349
XceI RCATGY 1 cut(s) 208
XspI CTAG 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.