FvH4_4g27941

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016832
Physical Location & Seq
Forward (+)
28724249 .. 28724744
496 bp
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UTR
Exon/CDS
Intron
FvH4_4g27941.t1

Sequence Viewer

Length: 330 bp
ATGCATCTGGGGCGATCATTTTCTCTGATATTCTTCTATGATATTAAACTCGAGCAACGTGTCCGAGAACTGAAAGAAGAAGTGAAAAGAATGCTAATGGCTGCAGTTAAAGAGCTTGCTTCGCACATGTTAGACATGGTGGATAACATTCAGCGTTTAGGCTTGTCATACAGTTTTGAAAATGAGATTGATACAATCTTGAAACACGTTCATGATCATTTTTATGGAAGTAAAGATCTTGGAAAGGATGGTGACCTTTACACTATTACTCTCCATTTTCGATTGCTTAGACAACAAGGCTATAATGTTTCATGCGGATATATGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

110

Amino Acids

12.87

Weight (kDa)

6.58

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 16 - 108 2e-27 Terpene synthase, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016832)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g27941
prunus_persica Prupe.4G238800_v2.0.a1
pyrus_communis pycom03g15650 pycom12g15550 pycom12g15650
rosa_chinensis RchiOBHm_Chr4g0399341
rosa_laevigata RLG00000035298
rosa_roxburghii Rroxscaffold_5G00344270
rosa_rugosa Rorug01G0225900 Rorug04G0016800
rosa_samantha Rh1AG240000 Rh5BG405500 Rh6AG030200 Rh7CG421600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 315
AflIII ACRYGT 3 cut(s) 58, 126, 205
AgsI TTSAA 2 cut(s) 179, 202
AluBI AGCT 1 cut(s) 115
AluI AGCT 1 cut(s) 115
Ama87I CYCGRG 1 cut(s) 50
ApeKI GCWGC 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 263
AvaI CYCGRG 1 cut(s) 50
BbvI GCAGC 1 cut(s) 88
BccI CCATC 1 cut(s) 242
BclI TGATCA 1 cut(s) 214
BfmI CTRYAG 1 cut(s) 102
BglII AGATCT 1 cut(s) 235
BisI GCNGC 1 cut(s) 102
BlsI GCNGC 1 cut(s) 103
BmeT110I CYCGRG 1 cut(s) 50
BmsI GCATC 1 cut(s) 13
BseGI GGATG 1 cut(s) 253
BseXI GCAGC 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 50
BsmI GAATGC 1 cut(s) 96
BsoBI CYCGRG 1 cut(s) 50
Bsp143I GATC 3 cut(s) 14, 214, 235
BspACI CCGC 1 cut(s) 315
BspHI TCATGA 1 cut(s) 211
BspMAI CTGCAG 1 cut(s) 106
BssMI GATC 3 cut(s) 14, 214, 235
Bst4CI ACNGT 1 cut(s) 173
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 287
BstEII GGTNACC 1 cut(s) 251
BstF5I GGATG 1 cut(s) 253
BstKTI GATC 3 cut(s) 17, 217, 238
BstMBI GATC 3 cut(s) 14, 214, 235
BstMWI GCNNNNNNNGC 2 cut(s) 10, 121
BstNSI RCATGY 1 cut(s) 130
BstPI GGTNACC 1 cut(s) 251
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BtsCI GGATG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 117
CciI TCATGA 1 cut(s) 211
CviAII CATG 4 cut(s) 127, 136, 212, 312
CviJI RGCY 4 cut(s) 101, 115, 162, 300
CviKI_1 RGCY 4 cut(s) 101, 115, 162, 300
DdeI CTNAG 1 cut(s) 287
DpnI GATC 3 cut(s) 16, 216, 237
DpnII GATC 3 cut(s) 14, 214, 235
Eco88I CYCGRG 1 cut(s) 50
Eco91I GGTNACC 1 cut(s) 251
EcoO65I GGTNACC 1 cut(s) 251
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 4 cut(s) 130, 139, 215, 315
FatI CATG 4 cut(s) 126, 135, 211, 311
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 1 cut(s) 102
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 1 cut(s) 102
GluI GCNGC 1 cut(s) 102
Hin1II CATG 4 cut(s) 130, 139, 215, 315
HphI GGTGA 1 cut(s) 263
Hpy188I TCNGA 2 cut(s) 27, 65
Hpy188III TCNNGA 2 cut(s) 199, 212
HpyCH4III ACNGT 1 cut(s) 173
HpyCH4IV ACGT 2 cut(s) 58, 207
HpyCH4V TGCA 2 cut(s) 4, 104
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 121
HpyF3I CTNAG 1 cut(s) 287
HpySE526I ACGT 2 cut(s) 58, 207
Hsp92II CATG 4 cut(s) 130, 139, 215, 315
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 3 cut(s) 14, 214, 235
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 1 cut(s) 13
MaeII ACGT 2 cut(s) 58, 207
MaeIII GTNAC 1 cut(s) 251
MalI GATC 3 cut(s) 16, 216, 237
MboI GATC 3 cut(s) 14, 214, 235
MboII GAAGA 2 cut(s) 25, 89
MflI RGATCY 1 cut(s) 235
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 45, 108
MslI CAYNNNNRTG 2 cut(s) 210, 222
Mva1269I GAATGC 1 cut(s) 96
MwoI GCNNNNNNNGC 2 cut(s) 10, 121
NdeII GATC 3 cut(s) 14, 214, 235
NlaIII CATG 4 cut(s) 130, 139, 215, 315
NmuCI GTSAC 1 cut(s) 251
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 130
PaeR7I CTCGAG 1 cut(s) 50
PagI TCATGA 1 cut(s) 211
PciI ACATGT 1 cut(s) 126
PctI GAATGC 1 cut(s) 96
PkrI GCNGC 1 cut(s) 103
PscI ACATGT 1 cut(s) 126
PspEI GGTNACC 1 cut(s) 251
PspXI VCTCGAGB 1 cut(s) 50
PstI CTGCAG 1 cut(s) 106
PsuI RGATCY 1 cut(s) 235
RseI CAYNNNNRTG 2 cut(s) 210, 222
SaqAI TTAA 2 cut(s) 45, 108
SatI GCNGC 1 cut(s) 102
Sau3AI GATC 3 cut(s) 14, 214, 235
SetI ASST 4 cut(s) 61, 117, 210, 258
SfaNI GCATC 1 cut(s) 13
SfcI CTRYAG 1 cut(s) 102
Sfr274I CTCGAG 1 cut(s) 50
SlaI CTCGAG 1 cut(s) 50
SmiMI CAYNNNNRTG 2 cut(s) 210, 222
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
SsiI CCGC 1 cut(s) 315
TaaI ACNGT 1 cut(s) 173
TaiI ACGT 2 cut(s) 61, 210
TaqI TCGA 2 cut(s) 51, 280
Tru1I TTAA 2 cut(s) 45, 108
Tru9I TTAA 2 cut(s) 45, 108
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 2 cut(s) 200, 300
XceI RCATGY 1 cut(s) 130
XhoI CTCGAG 1 cut(s) 50
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.