FvH4_4g29220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017657
Physical Location & Seq
Reverse (-)
29446884 .. 29448294
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29220.t1

Sequence Viewer

Length: 702 bp
ATGAGTGAAACTTCATCAAAACCGTCATTCTTTTTCCCACTAGAAATGCCAACTCAATTTTCATGGAGGAAATACGAGAACTCAGTTTTGTTCAAACTGCTGAAGTTGTGGGCAAAGGCAACCGTATGTGTAGTGGTGCTCCCCACAATCCTCTTCGTGTGCGTCCCACTCTCTATCCTTCTCATCCCACCTTTATTTATTGCTTCGACATGGGCGTTTTGTATGTGCAAGTTACTATATTTCTTGTGCCCCCTTAGAGTTGTAGACACCATGAAGTTTTTCAGATTCTTTACCACGTTAATATTAGGTGGCAGCAGCACAGCCAGCACAAGCAAGGCTAGTGATAACGACGATGAGGGAAATAAGGAGGAGGAGATGATAGCCAAGAGCCGGGAGGATAAGGAGCAGCCGATGAGCGTGGTTGAGTTGGACCCTTCGGAGGTGCCGTGTTTCTACGAGTCCGATGATCATGAGCAAGAAAACAAAGAAGAGCAGAACAATGTTAAGGAAGTCAAGGAAGAAGTGGGTAAAACCGGCATGTACGAAGAAGGGTCATGGCAAGTTTTGGTACCAAAGCCTCCAATACCCAAAAGGAAGAAGGAGAAACATACGTTCCACGAAGTTTTGTCATATTGGAAAAGCAAGGAGAAAGGAGAGGCAGCATCAGCTGGCACAAGAGAAGAAGATTATTGCAGCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

26.73

Weight (kDa)

5.22

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g29220
malus_domestica MD16G1055000.v1.1
prunus_persica Prupe.1G294400_v2.0.a1
rosa_laevigata RLG00000006451
rosa_multiflora Rmu_sc0004339.1_g000030
rosa_roxburghii Rroxscaffold_5G00378410
rosa_rugosa Rorug04G0299100
rosa_samantha Rh4AG353900 Rh4BG363800 Rh4CG378000 Rh4DG357800
rosa_wichuraiana Rw4G031040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 568
AccB1I GGYRCC 2 cut(s) 442, 568
AccI GTMKAC 1 cut(s) 264
AcuI CTGAAG 1 cut(s) 122
AfaI GTAC 2 cut(s) 542, 570
AfiI CCNNNNNNNGG 2 cut(s) 390, 439
AgsI TTSAA 1 cut(s) 94
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AluBI AGCT 1 cut(s) 668
AluI AGCT 1 cut(s) 668
Alw21I GWGCWC 1 cut(s) 141
ApeKI GCWGC 5 cut(s) 312, 315, 406, 659, 693
Asp700I GAANNNNTTC 1 cut(s) 278
Asp718I GGTACC 1 cut(s) 568
AspS9I GGNCC 1 cut(s) 430
AsuC2I CCSGG 1 cut(s) 392
AvaII GGWCC 1 cut(s) 430
BaeGI GKGCMC 1 cut(s) 251
BanI GGYRCC 2 cut(s) 442, 568
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 4 cut(s) 324, 327, 418, 671
BceAI ACGGC 1 cut(s) 430
BclI TGATCA 1 cut(s) 466
BcnI CCSGG 1 cut(s) 392
BfaI CTAG 3 cut(s) 41, 339, 700
BisI GCNGC 5 cut(s) 313, 316, 407, 660, 694
BlsI GCNGC 5 cut(s) 314, 317, 408, 661, 695
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 3 cut(s) 432, 444, 570
BmrFI CCNGG 1 cut(s) 392
BmsI GCATC 1 cut(s) 671
BpuMI CCSGG 1 cut(s) 392
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 390, 439
Bse118I RCCGGY 1 cut(s) 533
BseGI GGATG 1 cut(s) 183
BseLI CCNNNNNNNGG 2 cut(s) 390, 439
BseMII CTCAG 1 cut(s) 96
BseRI GAGGAG 2 cut(s) 383, 386
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 4 cut(s) 324, 327, 418, 671
BshNI GGYRCC 2 cut(s) 442, 568
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 2 cut(s) 391, 534
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 2 cut(s) 390, 439
BsmFI GGGAC 1 cut(s) 149
Bsp1286I GDGCHC 2 cut(s) 141, 251
Bsp143I GATC 1 cut(s) 466
BspCNI CTCAG 1 cut(s) 95
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 3 cut(s) 432, 444, 570
BspQI GCTCTTC 1 cut(s) 483
BspT107I GGYRCC 2 cut(s) 442, 568
BsrFI RCCGGY 1 cut(s) 533
BssAI RCCGGY 1 cut(s) 533
BssMI GATC 1 cut(s) 466
Bst4CI ACNGT 2 cut(s) 24, 124
Bst6I CTCTTC 2 cut(s) 158, 483
BstC8I GCNNGC 2 cut(s) 325, 670
BstDEI CTNAG 2 cut(s) 82, 254
BstF5I GGATG 1 cut(s) 183
BstKTI GATC 1 cut(s) 469
BstMBI GATC 1 cut(s) 466
BstMWI GCNNNNNNNGC 2 cut(s) 324, 665
BstNSI RCATGY 1 cut(s) 541
BstSCI CCNGG 1 cut(s) 390
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 4 cut(s) 324, 327, 418, 671
BtsCI GGATG 1 cut(s) 183
Cac8I GCNNGC 2 cut(s) 325, 670
CciI TCATGA 1 cut(s) 469
Cfr10I RCCGGY 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 430
CseI GACGC 1 cut(s) 151
Csp6I GTAC 2 cut(s) 541, 569
CviAII CATG 6 cut(s) 63, 210, 271, 470, 538, 555
CviJI RGCY 7 cut(s) 323, 338, 383, 390, 409, 577, 668
CviKI_1 RGCY 7 cut(s) 323, 338, 383, 390, 409, 577, 668
CviQI GTAC 2 cut(s) 541, 569
DdeI CTNAG 2 cut(s) 82, 254
DpnI GATC 1 cut(s) 468
DpnII GATC 1 cut(s) 466
Eam1104I CTCTTC 2 cut(s) 158, 483
EarI CTCTTC 2 cut(s) 158, 483
Eco47I GGWCC 1 cut(s) 430
Eco57I CTGAAG 1 cut(s) 122
FaeI CATG 6 cut(s) 66, 213, 274, 473, 541, 558
FaqI GGGAC 1 cut(s) 149
FatI CATG 6 cut(s) 62, 209, 270, 469, 537, 554
FbaI TGATCA 1 cut(s) 466
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 5 cut(s) 313, 316, 407, 660, 694
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 5 cut(s) 313, 316, 407, 660, 694
FspBI CTAG 3 cut(s) 41, 339, 700
GluI GCNGC 5 cut(s) 313, 316, 407, 660, 694
HapII CCGG 2 cut(s) 391, 534
HgaI GACGC 1 cut(s) 151
Hin1II CATG 6 cut(s) 66, 213, 274, 473, 541, 558
HinfI GANTC 2 cut(s) 285, 458
HpaII CCGG 2 cut(s) 391, 534
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 3 cut(s) 284, 439, 463
Hpy188III TCNNGA 1 cut(s) 470
Hpy8I GTNNAC 1 cut(s) 265
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 4 cut(s) 188, 444, 542, 592
HpyCH4III ACNGT 2 cut(s) 24, 124
HpyCH4IV ACGT 2 cut(s) 296, 611
HpyCH4V TGCA 2 cut(s) 228, 693
HpyF10VI GCNNNNNNNGC 2 cut(s) 324, 665
HpyF3I CTNAG 2 cut(s) 82, 254
HpySE526I ACGT 2 cut(s) 296, 611
Hsp92II CATG 6 cut(s) 66, 213, 274, 473, 541, 558
KpnI GGTACC 1 cut(s) 572
Ksp22I TGATCA 1 cut(s) 466
Kzo9I GATC 1 cut(s) 466
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 2 cut(s) 144, 403
LpnPI CCDG 4 cut(s) 337, 404, 547, 654
Lsp1109I GCAGC 4 cut(s) 324, 327, 418, 671
LweI GCATC 1 cut(s) 671
MaeI CTAG 3 cut(s) 41, 339, 700
MaeII ACGT 2 cut(s) 296, 611
MaeIII GTNAC 1 cut(s) 231
MalI GATC 1 cut(s) 468
MboI GATC 1 cut(s) 466
MboII GAAGA 7 cut(s) 145, 500, 530, 557, 607, 692, 695
MhlI GDGCHC 2 cut(s) 141, 251
MluCI AATT 1 cut(s) 56
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 408
MnlI CCTC 9 cut(s) 60, 161, 349, 361, 364, 388, 433, 588, 649
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 2 cut(s) 299, 504
MspA1I CMGCKG 1 cut(s) 668
MspI CCGG 2 cut(s) 391, 534
MspR9I CCNGG 1 cut(s) 392
MwoI GCNNNNNNNGC 2 cut(s) 324, 665
NciI CCSGG 1 cut(s) 392
NdeII GATC 1 cut(s) 466
NlaIII CATG 6 cut(s) 66, 213, 274, 473, 541, 558
NlaIV GGNNCC 3 cut(s) 432, 444, 570
NspI RCATGY 1 cut(s) 541
PagI TCATGA 1 cut(s) 469
PciSI GCTCTTC 1 cut(s) 483
PcsI WCGNNNNNNNCGW 2 cut(s) 212, 443
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 285
PkrI GCNGC 5 cut(s) 314, 317, 408, 661, 695
PleI GAGTC 1 cut(s) 466
PpsI GAGTC 1 cut(s) 466
PspN4I GGNNCC 3 cut(s) 432, 444, 570
PspPI GGNCC 1 cut(s) 430
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 2 cut(s) 542, 570
RsaNI GTAC 2 cut(s) 541, 569
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 2 cut(s) 299, 504
SatI GCNGC 5 cut(s) 313, 316, 407, 660, 694
Sau3AI GATC 1 cut(s) 466
Sau96I GGNCC 1 cut(s) 430
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 1 cut(s) 392
SduI GDGCHC 2 cut(s) 141, 251
SetI ASST 6 cut(s) 193, 299, 310, 444, 614, 670
SfaNI GCATC 1 cut(s) 671
SinI GGWCC 1 cut(s) 430
Sse9I AATT 1 cut(s) 56
SspI AATATT 1 cut(s) 303
SspMI CTAG 3 cut(s) 41, 339, 700
StyD4I CCNGG 1 cut(s) 390
TaaI ACNGT 2 cut(s) 24, 124
TaiI ACGT 2 cut(s) 299, 614
TaqI TCGA 1 cut(s) 206
TasI AATT 1 cut(s) 56
TfiI GAWTC 1 cut(s) 285
Tru1I TTAA 2 cut(s) 299, 504
Tru9I TTAA 2 cut(s) 299, 504
TseI GCWGC 5 cut(s) 312, 315, 406, 659, 693
TspDTI ATGAA 2 cut(s) 51, 287
VpaK11BI GGWCC 1 cut(s) 430
XceI RCATGY 1 cut(s) 541
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 3 cut(s) 41, 339, 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.