Rh4BG363800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0017657
Physical Location & Seq
Reverse (-)
52203038 .. 52205507
2470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG363800.1

Sequence Viewer

Length: 666 bp
ATGATGAGTGAAACTTCATCAAAACCGTCATCCTTTTTCCCACTAGAAATGCGAACTCAATTATCATGGAGGAAATACAAAAACTCAGTTTTGTTCAAACTGCTAAATTTGTGGGCAAAGGCAACCGTATGTGTAGTAGTGCTCCCCACAATCTTCTTCATTTGCGTCCCACTCTCTATCCTTCTCATCCCACCGTTATTTATTGCTTCTACATGGGCGTTTTGTATATGCAAGATACTATATTTCTTGTGCCCTCTTAGAGTTGTAGACACCATGAAGTTTTTCAGATTCTGTACCACATTAATATTAGGCAGCAGCAGCACAACCAAGGCTGGTGATAACGACAATGAGGAAAATATGATGAATGAGTTTATAGCCAAGAGCCTGGAGGATAAGGAGCAGTCGATGAGCGTGGTTGAGTTGGACCCCTCGGAGGTGCCGTGTTTCTACGAATCAGATGATCATGAGCAAGAAAACAAAGAAGAGCAGAATGTTAAGGAAGAAGTGGGTAAAATCGGCATGTACGAGGAAGGGTCATGGCAAGTTTTGGTACCAAAGCCAATACCCAAAAGGAAGAAGGACAAACATTCGTTCCATGAAGTTTTGTCATATTGGAAAAGCAAGGAGAAAGGAGAGGCAGCATCAGCTGGCATAAAAGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

25.38

Weight (kDa)

5.57

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g29220
malus_domestica MD16G1055000.v1.1
prunus_persica Prupe.1G294400_v2.0.a1
rosa_laevigata RLG00000006451
rosa_multiflora Rmu_sc0004339.1_g000030
rosa_roxburghii Rroxscaffold_5G00378410
rosa_rugosa Rorug04G0299100
rosa_samantha Rh4AG353900 Rh4BG363800 Rh4CG378000 Rh4DG357800
rosa_wichuraiana Rw4G031040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 550
AccB1I GGYRCC 2 cut(s) 436, 550
AccI GTMKAC 1 cut(s) 267
AcsI RAATTY 1 cut(s) 106
AfaI GTAC 3 cut(s) 295, 524, 552
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 1 cut(s) 647
AluI AGCT 1 cut(s) 647
Alw21I GWGCWC 1 cut(s) 144
AlwNI CAGNNNCTG 1 cut(s) 291
ApeKI GCWGC 4 cut(s) 312, 315, 318, 638
ApoI RAATTY 1 cut(s) 106
AseI ATTAAT 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 281
Asp718I GGTACC 1 cut(s) 550
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 347
AvaII GGWCC 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 254
BanI GGYRCC 2 cut(s) 436, 550
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 4 cut(s) 324, 327, 330, 650
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 1 cut(s) 386
BclI TGATCA 1 cut(s) 460
BfaI CTAG 1 cut(s) 44
BisI GCNGC 4 cut(s) 313, 316, 319, 639
BlsI GCNGC 4 cut(s) 314, 317, 320, 640
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 3 cut(s) 426, 438, 552
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 650
BpmI CTGGAG 1 cut(s) 407
BsaJI CCNNGG 2 cut(s) 327, 429
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 433
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 2 cut(s) 327, 429
BseGI GGATG 2 cut(s) 29, 186
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMII CTCAG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 4 cut(s) 324, 327, 330, 650
BshNI GGYRCC 2 cut(s) 436, 550
BsiHKAI GWGCWC 1 cut(s) 144
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 433
BsmFI GGGAC 1 cut(s) 152
Bsp1286I GDGCHC 2 cut(s) 144, 254
Bsp143I GATC 1 cut(s) 460
BspCNI CTCAG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 3 cut(s) 426, 438, 552
BspQI GCTCTTC 1 cut(s) 477
BspT107I GGYRCC 2 cut(s) 436, 550
BssECI CCNNGG 2 cut(s) 327, 429
BssMI GATC 1 cut(s) 460
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 3 cut(s) 27, 127, 195
Bst6I CTCTTC 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 649
BstDEI CTNAG 2 cut(s) 85, 257
BstF5I GGATG 2 cut(s) 29, 186
BstKTI GATC 1 cut(s) 463
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 2 cut(s) 318, 644
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 254
BstV1I GCAGC 4 cut(s) 324, 327, 330, 650
BstXI CCANNNNNNTGG 1 cut(s) 385
BtsCI GGATG 2 cut(s) 29, 186
Cac8I GCNNGC 1 cut(s) 649
CaiI CAGNNNCTG 1 cut(s) 291
CciI TCATGA 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 424
CseI GACGC 1 cut(s) 154
Csp6I GTAC 3 cut(s) 294, 523, 551
CviAII CATG 7 cut(s) 66, 213, 274, 464, 520, 537, 596
CviJI RGCY 5 cut(s) 332, 377, 384, 559, 647
CviKI_1 RGCY 5 cut(s) 332, 377, 384, 559, 647
CviQI GTAC 3 cut(s) 294, 523, 551
DdeI CTNAG 2 cut(s) 85, 257
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 7 cut(s) 69, 216, 277, 467, 523, 540, 599
FaqI GGGAC 1 cut(s) 152
FatI CATG 7 cut(s) 65, 212, 273, 463, 519, 536, 595
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 4 cut(s) 313, 316, 319, 639
FokI GGATG 2 cut(s) 16, 173
Fsp4HI GCNGC 4 cut(s) 313, 316, 319, 639
FspBI CTAG 1 cut(s) 44
GluI GCNGC 4 cut(s) 313, 316, 319, 639
GsuI CTGGAG 1 cut(s) 407
HgaI GACGC 1 cut(s) 154
Hin1II CATG 7 cut(s) 69, 216, 277, 467, 523, 540, 599
HinfI GANTC 2 cut(s) 288, 452
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 3 cut(s) 287, 433, 457
Hpy188III TCNNGA 1 cut(s) 464
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 3 cut(s) 191, 524, 571
HpyCH4III ACNGT 3 cut(s) 27, 127, 195
HpyCH4V TGCA 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 318, 644
HpyF3I CTNAG 2 cut(s) 85, 257
Hsp92II CATG 7 cut(s) 69, 216, 277, 467, 523, 540, 599
KpnI GGTACC 1 cut(s) 554
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 1 cut(s) 460
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 2 cut(s) 147, 397
LpnPI CCDG 4 cut(s) 318, 371, 398, 633
Lsp1109I GCAGC 4 cut(s) 324, 327, 330, 650
LweI GCATC 1 cut(s) 650
MaeI CTAG 1 cut(s) 44
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 5 cut(s) 145, 148, 494, 512, 586
MhlI GDGCHC 2 cut(s) 144, 254
MluCI AATT 2 cut(s) 59, 106
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 8 cut(s) 63, 264, 343, 382, 427, 439, 520, 628
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 3 cut(s) 302, 495, 664
MspA1I CMGCKG 1 cut(s) 647
MspR9I CCNGG 1 cut(s) 386
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 318, 644
NdeII GATC 1 cut(s) 460
NlaIII CATG 7 cut(s) 69, 216, 277, 467, 523, 540, 599
NlaIV GGNNCC 3 cut(s) 426, 438, 552
NspI RCATGY 1 cut(s) 523
PagI TCATGA 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 2 cut(s) 437, 522
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 288, 452
PkrI GCNGC 4 cut(s) 314, 317, 320, 640
PshBI ATTAAT 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 3 cut(s) 426, 438, 552
PspPI GGNCC 1 cut(s) 424
PstNI CAGNNNCTG 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 3 cut(s) 295, 524, 552
RsaNI GTAC 3 cut(s) 294, 523, 551
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 3 cut(s) 302, 495, 664
SatI GCNGC 4 cut(s) 313, 316, 319, 639
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 2 cut(s) 144, 254
SetI ASST 2 cut(s) 438, 649
SfaNI GCATC 1 cut(s) 650
SinI GGWCC 1 cut(s) 424
Sse9I AATT 2 cut(s) 59, 106
SspI AATATT 1 cut(s) 306
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 3 cut(s) 27, 127, 195
TaqI TCGA 1 cut(s) 404
TasI AATT 2 cut(s) 59, 106
TfiI GAWTC 2 cut(s) 288, 452
Tru1I TTAA 3 cut(s) 302, 495, 664
Tru9I TTAA 3 cut(s) 302, 495, 664
TseI GCWGC 4 cut(s) 312, 315, 318, 638
TspDTI ATGAA 5 cut(s) 6, 148, 290, 377, 612
VpaK11BI GGWCC 1 cut(s) 424
VspI ATTAAT 1 cut(s) 302
XapI RAATTY 1 cut(s) 106
XceI RCATGY 1 cut(s) 523
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.