Rh4AG353900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017657
Physical Location & Seq
Reverse (-)
66297288 .. 66299776
2489 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG353900.1

Sequence Viewer

Length: 666 bp
ATGAGTGAAACTTCATCAAAACCGTCATCCTTTTTCCCACTAGAAATGCGAACTCAATTAACATGGAGGAAATACAAAAACTCAGTTTTGTTCAAACTGCTAAATTTGTGGGCAAAGGCAACCGTATGTGTAGTACTGCTCCCCACAATCTTCTTCATTTGCGTCCCACTCTCTATCCTCCTCATCCCACCGTTATTTATTGCTTCTACATGGGCGTTTTGTATATGCAAGATACTATATTTCTTGTGCCCTCTTAGAGTTGTAGACACCATGAAGTTTTTCAGATTCTGTACCACATTAATATTAGGCAGCAGCAGCACAACCAAGGCTGGTGATAACGACAATGAGGAAAATATGATGAAGGAGTTTATAGCCAAGAGCCTGGAGGATAAGGAGCAGTCGATGAGCGTGGTTGAGTTGGACCCCTCGGAGGTGCCGTGTTTCTACGAATCAGATGATCATGAGCAAGAAAACAAAGAAGAGCAGAATGTTAAGGAAGAAGTGGGTAAAATCGGCATGTACGAGGAAGGGTCATGGCAAGTTTTGGTACCAAAGCCTCCAATACCCAAAAGGAAGAAAGACAAACATTCGTTCCATGAAGTTTTGTCATATTGGAAAAGCAAGGAGAAAGGAGAGGCAGCATCAGCTGGCATAAAAGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

25.39

Weight (kDa)

5.78

Isoelectric Point (pI)

39.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g29220
malus_domestica MD16G1055000.v1.1
prunus_persica Prupe.1G294400_v2.0.a1
rosa_laevigata RLG00000006451
rosa_multiflora Rmu_sc0004339.1_g000030
rosa_roxburghii Rroxscaffold_5G00378410
rosa_rugosa Rorug04G0299100
rosa_samantha Rh4AG353900 Rh4BG363800 Rh4CG378000 Rh4DG357800
rosa_wichuraiana Rw4G031040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 547
AccB1I GGYRCC 2 cut(s) 433, 547
AccI GTMKAC 1 cut(s) 264
AcsI RAATTY 1 cut(s) 103
AfaI GTAC 4 cut(s) 135, 292, 521, 549
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 1 cut(s) 94
AjnI CCWGG 1 cut(s) 381
AluBI AGCT 1 cut(s) 647
AluI AGCT 1 cut(s) 647
AlwNI CAGNNNCTG 1 cut(s) 288
ApeKI GCWGC 4 cut(s) 309, 312, 315, 638
ApoI RAATTY 1 cut(s) 103
AseI ATTAAT 1 cut(s) 299
Asp700I GAANNNNTTC 1 cut(s) 278
Asp718I GGTACC 1 cut(s) 547
AspS9I GGNCC 1 cut(s) 421
AsuHPI GGTGA 1 cut(s) 344
AvaII GGWCC 1 cut(s) 421
BaeGI GKGCMC 1 cut(s) 251
BanI GGYRCC 2 cut(s) 433, 547
BbvI GCAGC 4 cut(s) 321, 324, 327, 650
BceAI ACGGC 1 cut(s) 421
BciT130I CCWGG 1 cut(s) 383
BclI TGATCA 1 cut(s) 457
BfaI CTAG 1 cut(s) 41
BisI GCNGC 4 cut(s) 310, 313, 316, 639
BlsI GCNGC 4 cut(s) 311, 314, 317, 640
BmcAI AGTACT 1 cut(s) 135
Bme1390I CCNGG 1 cut(s) 383
Bme18I GGWCC 1 cut(s) 421
BmgT120I GGNCC 1 cut(s) 421
BmiI GGNNCC 3 cut(s) 423, 435, 549
BmrFI CCNGG 1 cut(s) 383
BmsI GCATC 1 cut(s) 650
BpmI CTGGAG 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 324, 426
BsaXI ACNNNNNCTCC 2 cut(s) 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 430
BseBI CCWGG 1 cut(s) 383
BseDI CCNNGG 2 cut(s) 324, 426
BseGI GGATG 2 cut(s) 26, 183
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMII CTCAG 1 cut(s) 96
BseRI GAGGAG 1 cut(s) 170
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 4 cut(s) 321, 324, 327, 650
BshNI GGYRCC 2 cut(s) 433, 547
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 1 cut(s) 430
BsmFI GGGAC 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 1 cut(s) 457
BspCNI CTCAG 1 cut(s) 95
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 3 cut(s) 423, 435, 549
BspQI GCTCTTC 1 cut(s) 474
BspT107I GGYRCC 2 cut(s) 433, 547
BssECI CCNNGG 2 cut(s) 324, 426
BssMI GATC 1 cut(s) 457
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 383
Bst4CI ACNGT 3 cut(s) 24, 124, 192
Bst6I CTCTTC 1 cut(s) 474
BstC8I GCNNGC 1 cut(s) 649
BstDEI CTNAG 2 cut(s) 82, 254
BstF5I GGATG 2 cut(s) 26, 183
BstKTI GATC 1 cut(s) 460
BstMBI GATC 1 cut(s) 457
BstMWI GCNNNNNNNGC 2 cut(s) 315, 644
BstNI CCWGG 1 cut(s) 383
BstNSI RCATGY 1 cut(s) 520
BstSCI CCNGG 1 cut(s) 381
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 4 cut(s) 321, 324, 327, 650
BstXI CCANNNNNNTGG 1 cut(s) 382
BtsCI GGATG 2 cut(s) 26, 183
Cac8I GCNNGC 1 cut(s) 649
CaiI CAGNNNCTG 1 cut(s) 288
CciI TCATGA 1 cut(s) 460
Cfr13I GGNCC 1 cut(s) 421
CseI GACGC 1 cut(s) 151
Csp6I GTAC 4 cut(s) 134, 291, 520, 548
CviAII CATG 7 cut(s) 63, 210, 271, 461, 517, 534, 596
CviJI RGCY 5 cut(s) 329, 374, 381, 556, 647
CviKI_1 RGCY 5 cut(s) 329, 374, 381, 556, 647
CviQI GTAC 4 cut(s) 134, 291, 520, 548
DdeI CTNAG 2 cut(s) 82, 254
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 381
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 7 cut(s) 66, 213, 274, 464, 520, 537, 599
FaqI GGGAC 1 cut(s) 149
FatI CATG 7 cut(s) 62, 209, 270, 460, 516, 533, 595
FbaI TGATCA 1 cut(s) 457
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 4 cut(s) 310, 313, 316, 639
FokI GGATG 2 cut(s) 13, 170
Fsp4HI GCNGC 4 cut(s) 310, 313, 316, 639
FspBI CTAG 1 cut(s) 41
GluI GCNGC 4 cut(s) 310, 313, 316, 639
GsuI CTGGAG 1 cut(s) 404
HgaI GACGC 1 cut(s) 151
Hin1II CATG 7 cut(s) 66, 213, 274, 464, 520, 537, 599
HinfI GANTC 2 cut(s) 285, 449
HphI GGTGA 1 cut(s) 344
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 3 cut(s) 284, 430, 454
Hpy188III TCNNGA 1 cut(s) 461
Hpy8I GTNNAC 1 cut(s) 265
HpyAV CCTTC 2 cut(s) 355, 521
HpyCH4III ACNGT 3 cut(s) 24, 124, 192
HpyCH4V TGCA 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 644
HpyF3I CTNAG 2 cut(s) 82, 254
Hsp92II CATG 7 cut(s) 66, 213, 274, 464, 520, 537, 599
KpnI GGTACC 1 cut(s) 551
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 1 cut(s) 457
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 2 cut(s) 144, 394
LpnPI CCDG 4 cut(s) 315, 368, 395, 633
Lsp1109I GCAGC 4 cut(s) 321, 324, 327, 650
LweI GCATC 1 cut(s) 650
MaeI CTAG 1 cut(s) 41
MalI GATC 1 cut(s) 459
MboI GATC 1 cut(s) 457
MboII GAAGA 5 cut(s) 142, 145, 491, 509, 586
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 2 cut(s) 56, 103
MmeI TCCRAC 1 cut(s) 399
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 4 cut(s) 59, 299, 492, 664
MspA1I CMGCKG 1 cut(s) 647
MspR9I CCNGG 1 cut(s) 383
MvaI CCWGG 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 315, 644
NdeII GATC 1 cut(s) 457
NlaIII CATG 7 cut(s) 66, 213, 274, 464, 520, 537, 599
NlaIV GGNNCC 3 cut(s) 423, 435, 549
NspI RCATGY 1 cut(s) 520
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 474
PcsI WCGNNNNNNNCGW 2 cut(s) 434, 519
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 2 cut(s) 285, 449
PkrI GCNGC 4 cut(s) 311, 314, 317, 640
PshBI ATTAAT 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 381
PspGI CCWGG 1 cut(s) 381
PspN4I GGNNCC 3 cut(s) 423, 435, 549
PspPI GGNCC 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 647
RsaI GTAC 4 cut(s) 135, 292, 521, 549
RsaNI GTAC 4 cut(s) 134, 291, 520, 548
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 4 cut(s) 59, 299, 492, 664
SatI GCNGC 4 cut(s) 310, 313, 316, 639
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 1 cut(s) 421
ScaI AGTACT 1 cut(s) 135
ScrFI CCNGG 1 cut(s) 383
SduI GDGCHC 1 cut(s) 251
SetI ASST 2 cut(s) 435, 649
SfaNI GCATC 1 cut(s) 650
SinI GGWCC 1 cut(s) 421
Sse9I AATT 2 cut(s) 56, 103
SspI AATATT 1 cut(s) 303
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 381
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 3 cut(s) 24, 124, 192
TaqI TCGA 1 cut(s) 401
TasI AATT 2 cut(s) 56, 103
TatI WGTACW 1 cut(s) 133
TfiI GAWTC 2 cut(s) 285, 449
Tru1I TTAA 4 cut(s) 59, 299, 492, 664
Tru9I TTAA 4 cut(s) 59, 299, 492, 664
TseI GCWGC 4 cut(s) 309, 312, 315, 638
TspDTI ATGAA 4 cut(s) 145, 287, 374, 612
VpaK11BI GGWCC 1 cut(s) 421
VspI ATTAAT 1 cut(s) 299
XapI RAATTY 1 cut(s) 103
XceI RCATGY 1 cut(s) 520
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 1 cut(s) 41
ZrmI AGTACT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.