Rw4G031040

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017657
Physical Location & Seq
Reverse (-)
57929945 .. 57931093
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031040.1

Sequence Viewer

Length: 669 bp
ATGATGAGTGAAACTTCATCAAAACCGCCATCCTTTTTCCCACTAGAAATGCGAACTCAATTAACATGGAGGAAATACAAAAACTCAGTTTTGTTCAAACTGCTAAATTTGTGGGCAAAGGCAACCGTATGTGTAGTAGTGCTCCCCACAATCTTCTTCATTTGCGTCCCACTCTCTATCCTCCTCCTCCCACCGTTATTTATTGCTTCTACATGGGCGTTTTGTATATGCAAGATACTATATTTCTTGTGCCCTCTTAGAGTTGTAGACACCATGAAGTTTTTCAGATTCTGTACCACATTAATATTAGGCAGCAGCAGCACAACCAAGGCTGGTGATAACGACAATGAGGAAAATATGATGAAGGAGTTTATAGCCAAGAGCCTGGAGGATAAGGAGCAGTCGATGAGCGTGGTTGAGTTGGACCCCTCGGAGGTGCCGTGTTTCTACGAATCAGATGATCATGAGCAAGAAAACAAAGAAGAGCAGAATGTTAAGGAAGAAGTGGGTAAAATCGGCATGTACGAGGAAGGGTCATGGCAAGTTTTGGTACCAAAGCCTCCAATACCCAAAAGGAAGAAAGACAAACATTCGTTCCATGAAGTTTTGTCATATTGGAAAAGCAAGGAGAAAGGACAGGCAGCATCAGCTGGCATAAAAGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.51

Weight (kDa)

6.02

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017657)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g29220
malus_domestica MD16G1055000.v1.1
prunus_persica Prupe.1G294400_v2.0.a1
rosa_laevigata RLG00000006451
rosa_multiflora Rmu_sc0004339.1_g000030
rosa_roxburghii Rroxscaffold_5G00378410
rosa_rugosa Rorug04G0299100
rosa_samantha Rh4AG353900 Rh4BG363800 Rh4CG378000 Rh4DG357800
rosa_wichuraiana Rw4G031040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 550
AccB1I GGYRCC 2 cut(s) 436, 550
AccI GTMKAC 1 cut(s) 267
AciI CCGC 1 cut(s) 26
AcsI RAATTY 1 cut(s) 106
AfaI GTAC 3 cut(s) 295, 524, 552
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 1 cut(s) 650
AluI AGCT 1 cut(s) 650
Alw21I GWGCWC 1 cut(s) 144
AlwNI CAGNNNCTG 1 cut(s) 291
ApeKI GCWGC 4 cut(s) 312, 315, 318, 641
ApoI RAATTY 1 cut(s) 106
AseI ATTAAT 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 281
Asp718I GGTACC 1 cut(s) 550
AspS9I GGNCC 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 347
AvaII GGWCC 1 cut(s) 424
BaeGI GKGCMC 1 cut(s) 254
BanI GGYRCC 2 cut(s) 436, 550
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 4 cut(s) 324, 327, 330, 653
BccI CCATC 1 cut(s) 37
BceAI ACGGC 1 cut(s) 424
BciT130I CCWGG 1 cut(s) 386
BclI TGATCA 1 cut(s) 460
BfaI CTAG 1 cut(s) 44
BisI GCNGC 4 cut(s) 313, 316, 319, 642
BlsI GCNGC 4 cut(s) 314, 317, 320, 643
Bme1390I CCNGG 1 cut(s) 386
Bme18I GGWCC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 424
BmiI GGNNCC 3 cut(s) 426, 438, 552
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 1 cut(s) 653
BpmI CTGGAG 1 cut(s) 407
BsaJI CCNNGG 2 cut(s) 327, 429
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 433
BseBI CCWGG 1 cut(s) 386
BseDI CCNNGG 2 cut(s) 327, 429
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 2 cut(s) 173, 176
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 4 cut(s) 324, 327, 330, 653
BshNI GGYRCC 2 cut(s) 436, 550
BsiHKAI GWGCWC 1 cut(s) 144
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 433
BsmFI GGGAC 1 cut(s) 152
Bsp1286I GDGCHC 2 cut(s) 144, 254
Bsp143I GATC 1 cut(s) 460
BspACI CCGC 1 cut(s) 26
BspCNI CTCAG 1 cut(s) 98
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 3 cut(s) 426, 438, 552
BspQI GCTCTTC 1 cut(s) 477
BspT107I GGYRCC 2 cut(s) 436, 550
BssECI CCNNGG 2 cut(s) 327, 429
BssMI GATC 1 cut(s) 460
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 2 cut(s) 127, 195
Bst6I CTCTTC 1 cut(s) 477
BstC8I GCNNGC 1 cut(s) 652
BstDEI CTNAG 2 cut(s) 85, 257
BstF5I GGATG 1 cut(s) 29
BstKTI GATC 1 cut(s) 463
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 2 cut(s) 318, 647
BstNI CCWGG 1 cut(s) 386
BstNSI RCATGY 1 cut(s) 523
BstSCI CCNGG 1 cut(s) 384
BstSLI GKGCMC 1 cut(s) 254
BstV1I GCAGC 4 cut(s) 324, 327, 330, 653
BstXI CCANNNNNNTGG 1 cut(s) 385
BtsCI GGATG 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 652
CaiI CAGNNNCTG 1 cut(s) 291
CciI TCATGA 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 424
CseI GACGC 1 cut(s) 154
Csp6I GTAC 3 cut(s) 294, 523, 551
CviAII CATG 7 cut(s) 66, 213, 274, 464, 520, 537, 599
CviJI RGCY 5 cut(s) 332, 377, 384, 559, 650
CviKI_1 RGCY 5 cut(s) 332, 377, 384, 559, 650
CviQI GTAC 3 cut(s) 294, 523, 551
DdeI CTNAG 2 cut(s) 85, 257
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 424
EcoRII CCWGG 1 cut(s) 384
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 7 cut(s) 69, 216, 277, 467, 523, 540, 602
FaqI GGGAC 1 cut(s) 152
FatI CATG 7 cut(s) 65, 212, 273, 463, 519, 536, 598
FbaI TGATCA 1 cut(s) 460
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 4 cut(s) 313, 316, 319, 642
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 4 cut(s) 313, 316, 319, 642
FspBI CTAG 1 cut(s) 44
GluI GCNGC 4 cut(s) 313, 316, 319, 642
GsuI CTGGAG 1 cut(s) 407
HgaI GACGC 1 cut(s) 154
Hin1II CATG 7 cut(s) 69, 216, 277, 467, 523, 540, 602
HinfI GANTC 2 cut(s) 288, 452
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 3 cut(s) 287, 433, 457
Hpy188III TCNNGA 1 cut(s) 464
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 2 cut(s) 358, 524
HpyCH4III ACNGT 2 cut(s) 127, 195
HpyCH4V TGCA 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 2 cut(s) 318, 647
HpyF3I CTNAG 2 cut(s) 85, 257
Hsp92II CATG 7 cut(s) 69, 216, 277, 467, 523, 540, 602
KpnI GGTACC 1 cut(s) 554
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 1 cut(s) 460
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 2 cut(s) 147, 397
LpnPI CCDG 5 cut(s) 318, 371, 398, 623, 636
Lsp1109I GCAGC 4 cut(s) 324, 327, 330, 653
LweI GCATC 1 cut(s) 653
MaeI CTAG 1 cut(s) 44
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 5 cut(s) 145, 148, 494, 512, 589
MhlI GDGCHC 2 cut(s) 144, 254
MluCI AATT 2 cut(s) 59, 106
MmeI TCCRAC 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 4 cut(s) 62, 302, 495, 667
MspA1I CMGCKG 1 cut(s) 650
MspR9I CCNGG 1 cut(s) 386
MvaI CCWGG 1 cut(s) 386
MwoI GCNNNNNNNGC 2 cut(s) 318, 647
NdeII GATC 1 cut(s) 460
NlaIII CATG 7 cut(s) 69, 216, 277, 467, 523, 540, 602
NlaIV GGNNCC 3 cut(s) 426, 438, 552
NspI RCATGY 1 cut(s) 523
PagI TCATGA 1 cut(s) 463
PciSI GCTCTTC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 2 cut(s) 437, 522
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 288, 452
PkrI GCNGC 4 cut(s) 314, 317, 320, 643
PshBI ATTAAT 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspN4I GGNNCC 3 cut(s) 426, 438, 552
PspPI GGNCC 1 cut(s) 424
PstNI CAGNNNCTG 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 3 cut(s) 295, 524, 552
RsaNI GTAC 3 cut(s) 294, 523, 551
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 4 cut(s) 62, 302, 495, 667
SatI GCNGC 4 cut(s) 313, 316, 319, 642
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 1 cut(s) 424
ScrFI CCNGG 1 cut(s) 386
SduI GDGCHC 2 cut(s) 144, 254
SetI ASST 2 cut(s) 438, 652
SfaNI GCATC 1 cut(s) 653
SinI GGWCC 1 cut(s) 424
Sse9I AATT 2 cut(s) 59, 106
SsiI CCGC 1 cut(s) 26
SspI AATATT 1 cut(s) 306
SspMI CTAG 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 384
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 2 cut(s) 127, 195
TaqI TCGA 1 cut(s) 404
TasI AATT 2 cut(s) 59, 106
TfiI GAWTC 2 cut(s) 288, 452
Tru1I TTAA 4 cut(s) 62, 302, 495, 667
Tru9I TTAA 4 cut(s) 62, 302, 495, 667
TseI GCWGC 4 cut(s) 312, 315, 318, 641
TspDTI ATGAA 5 cut(s) 6, 148, 290, 377, 615
VpaK11BI GGWCC 1 cut(s) 424
VspI ATTAAT 1 cut(s) 302
XapI RAATTY 1 cut(s) 106
XceI RCATGY 1 cut(s) 523
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 281
XspI CTAG 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.