FvH4_4g30470

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013346
Physical Location & Seq
Forward (+)
30070534 .. 30072371
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30470.t1

Sequence Viewer

Length: 720 bp
ATGGAGATCGATCAATCGGAGCCGCAAACCGAAAGCCAAGACCTCTTCAAAGCCGCCGAGACCGGCGAGTCTTCGGCGTTCGAATCCCTCCCACCAAAGCAGCTCGCTTTAGCTTCCGCTCTCAGAAACGAGGACGGCCGCTCCCTCCTTCACGTCGCCGTCTCTTCCGGCCAATCTCAGGTGGTGAAGATACTACTAGCTGGTGAAGAATCCAGCAGCATGATAAACAGCAAGGATGAAGATGGCTGGGCGCCGCTTCATTCCGCGGCGAGCATTGGGAACACAGAAATAACCGAGATGCTGCTCAGCAAAGGAGCTGATGTTAATTTGAAGAATGATGGTGGCCGCGTTGCGCTTCACTATGCTGCCAGCAAGGGATGGATGGAGATTGCGGAGAATTTGATCTCGCACGGGGCAAAGCTTAATGCACACGACAAGGTTGGTTCGACCCCATTGCATAGAGCAGCTAGTACTGGGAAAGCACAATTGTGTGAATTTTTAATTGAGGAAGGAGCTGACATAGATGTTGTTGATAGAGCAGGCCAGACTCCTCTTATGAGTGCTGTGATTTGCGAGAACAAGGAGGTTTCTCTTCTTCTAATAAGGCACGGAGCAGATGTGGATGTGGAAGACAAAGAAGGATACACAGTGCTTGGTCGAGCTTCAAATGAGTTTAGACCATTATTGATCGATGCCGCCAAGGCAATGCTTGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

240

Amino Acids

25.45

Weight (kDa)

4.74

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 112 6.7e-15 Ankyrin repeats (3 copies)
Ank PF00023 81 - 113 2.1e-08 Ankyrin repeat
Ank_4 PF13637 93 - 132 1.9e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 101 - 155 6.6e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 110 - 178 2.2e-13 Ankyrin repeats (3 copies)
Ank PF00023 115 - 146 8.2e-06 Ankyrin repeat
Ank_4 PF13637 119 - 168 8.3e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 134 - 185 4.6e-07 Ankyrin repeats (many copies)
Ank PF00023 148 - 179 6.2e-07 Ankyrin repeat
Ank_4 PF13637 152 - 201 3.3e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 160 - 222 1.8e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03430
fragaria_vesca FvH4_4g30470
malus_domestica MD13G1065800.v1.1 MD16G1064400.v1.1
prunus_persica Prupe.1G282400_v2.0.a1
pyrus_communis pycom13g05800 pycom16g05620
rosa_chinensis RchiOBHm_Chr4g0438821
rosa_laevigata RLG00000006328
rosa_multiflora Rmu_sc0002505.1_g000016
rosa_roxburghii Rroxscaffold_5G00379860
rosa_rugosa Rorug04G0313300
rosa_samantha Rh4AG364300 Rh4BG375400 Rh4CG389500 Rh4DG370000
rosa_wichuraiana Rw0G007240 Rw0G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 67
AccB1I GGYRCC 1 cut(s) 250
AccBSI CCGCTC 2 cut(s) 119, 141
AccII CGCG 2 cut(s) 266, 348
AcoI YGGCCR 3 cut(s) 136, 169, 343
AcsI RAATTY 2 cut(s) 397, 494
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 1 cut(s) 472
AfiI CCNNNNNNNGG 1 cut(s) 178
AgsI TTSAA 4 cut(s) 49, 331, 666, 713
AjiI CACGTC 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 487
AluBI AGCT 8 cut(s) 103, 113, 200, 317, 421, 467, 515, 662
AluI AGCT 8 cut(s) 103, 113, 200, 317, 421, 467, 515, 662
Alw26I GTCTC 2 cut(s) 53, 166
AoxI GGCC 4 cut(s) 136, 169, 343, 541
ApeKI GCWGC 5 cut(s) 100, 216, 301, 365, 464
ApoI RAATTY 2 cut(s) 397, 494
AspLEI GCGC 2 cut(s) 253, 355
AsuHPI GGTGA 2 cut(s) 196, 215
AsuII TTCGAA 1 cut(s) 81
BanI GGYRCC 1 cut(s) 250
BbsI GAAGAC 2 cut(s) 63, 636
BbvI GCAGC 5 cut(s) 112, 228, 288, 352, 476
BccI CCATC 4 cut(s) 236, 332, 372, 376
BceAI ACGGC 2 cut(s) 143, 151
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciVI GTATCC 1 cut(s) 635
BcoDI GTCTC 2 cut(s) 53, 166
BfaI CTAG 2 cut(s) 197, 468
BfoI RGCGCY 1 cut(s) 254
BfuI GTATCC 1 cut(s) 635
BglI GCCNNNNNGGC 1 cut(s) 701
BlpI GCTNAGC 1 cut(s) 305
BmcAI AGTACT 1 cut(s) 472
BmgBI CACGTC 1 cut(s) 154
BmiI GGNNCC 2 cut(s) 21, 252
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 288, 682
BmuI ACTGGG 1 cut(s) 483
BpiI GAAGAC 2 cut(s) 63, 636
Bpu1102I GCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 1 cut(s) 81
Bsa29I ATCGAT 2 cut(s) 9, 690
BsaHI GRCGYC 1 cut(s) 251
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 264, 699
BsaXI ACNNNNNCTCC 6 cut(s) 125, 155, 306, 336, 603, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 2 cut(s) 452, 711
BseCI ATCGAT 2 cut(s) 9, 690
BseDI CCNNGG 2 cut(s) 264, 699
BseGI GGATG 4 cut(s) 241, 383, 387, 628
BseLI CCNNNNNNNGG 1 cut(s) 178
BseMI GCAATG 2 cut(s) 452, 711
BseMII CTCAG 3 cut(s) 136, 191, 319
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 540
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 5 cut(s) 112, 228, 288, 352, 476
BseYI CCCAGC 1 cut(s) 246
Bsh1236I CGCG 2 cut(s) 266, 348
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 4 cut(s) 138, 171, 345, 543
BshNI GGYRCC 1 cut(s) 250
BshVI ATCGAT 2 cut(s) 9, 690
BsiEI CGRYCG 1 cut(s) 139
BsiSI CCGG 2 cut(s) 63, 168
BslI CCNNNNNNNGG 1 cut(s) 178
BsmAI GTCTC 2 cut(s) 53, 166
BsmBI CGTCTC 1 cut(s) 166
BsnI GGCC 4 cut(s) 138, 171, 345, 543
Bso31I GGTCTC 1 cut(s) 53
Bsp119I TTCGAA 1 cut(s) 81
Bsp143I GATC 4 cut(s) 6, 10, 402, 687
Bsp1720I GCTNAGC 1 cut(s) 305
BspANI GGCC 4 cut(s) 138, 171, 345, 543
BspCNI CTCAG 3 cut(s) 135, 190, 318
BspDI ATCGAT 2 cut(s) 9, 690
BspFNI CGCG 2 cut(s) 266, 348
BspLI GGNNCC 2 cut(s) 21, 252
BspT104I TTCGAA 1 cut(s) 81
BspT107I GGYRCC 1 cut(s) 250
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 2 cut(s) 119, 141
BsrDI GCAATG 2 cut(s) 452, 711
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 1 cut(s) 478
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 264, 699
BssMI GATC 4 cut(s) 6, 10, 402, 687
BssNI GRCGYC 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 699
Bst4CI ACNGT 1 cut(s) 649
Bst6I CTCTTC 3 cut(s) 50, 169, 597
BstACI GRCGYC 1 cut(s) 251
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 4 cut(s) 105, 271, 370, 541
BstDEI CTNAG 3 cut(s) 122, 177, 305
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 4 cut(s) 241, 383, 387, 628
BstFNI CGCG 2 cut(s) 266, 348
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 2 cut(s) 253, 355
BstKTI GATC 4 cut(s) 9, 13, 405, 690
BstMAI GTCTC 2 cut(s) 53, 166
BstMBI GATC 4 cut(s) 6, 10, 402, 687
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 701
BstUI CGCG 2 cut(s) 266, 348
BstV1I GCAGC 5 cut(s) 112, 228, 288, 352, 476
BstV2I GAAGAC 2 cut(s) 63, 636
BstZI CGGCCG 1 cut(s) 136
Bsu15I ATCGAT 2 cut(s) 9, 690
BsuI GTATCC 1 cut(s) 635
BsuRI GGCC 4 cut(s) 138, 171, 345, 543
BsuTUI ATCGAT 2 cut(s) 9, 690
BtgI CCRYGG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 4 cut(s) 241, 383, 387, 628
BtsIMutI CAGTG 1 cut(s) 654
Cac8I GCNNGC 4 cut(s) 105, 271, 370, 541
CfoI GCGC 2 cut(s) 253, 355
Cfr10I RCCGGY 1 cut(s) 62
Cfr42I CCGCGG 1 cut(s) 267
ClaI ATCGAT 2 cut(s) 9, 690
Csp6I GTAC 1 cut(s) 471
CviAII CATG 1 cut(s) 220
CviQI GTAC 1 cut(s) 471
DdeI CTNAG 3 cut(s) 122, 177, 305
DinI GGCGCC 1 cut(s) 252
DpnI GATC 4 cut(s) 8, 12, 404, 689
DpnII GATC 4 cut(s) 6, 10, 402, 687
DrdI GACNNNNNNGTC 1 cut(s) 67
DseDI GACNNNNNNGTC 1 cut(s) 67
EaeI YGGCCR 3 cut(s) 136, 169, 343
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 3 cut(s) 50, 169, 597
EarI CTCTTC 3 cut(s) 50, 169, 597
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 53
Eco52I CGGCCG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 699
EgeI GGCGCC 1 cut(s) 252
EheI GGCGCC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 1 cut(s) 223
FaiI YATR 5 cut(s) 221, 363, 459, 521, 557
FatI CATG 1 cut(s) 219
FokI GGATG 4 cut(s) 248, 390, 394, 635
FspBI CTAG 2 cut(s) 197, 468
GlaI GCGC 2 cut(s) 252, 354
GsaI CCCAGC 1 cut(s) 250
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 4 cut(s) 138, 171, 345, 543
HapII CCGG 2 cut(s) 63, 168
HhaI GCGC 2 cut(s) 253, 355
Hin1I GRCGYC 1 cut(s) 251
Hin1II CATG 1 cut(s) 223
Hin6I GCGC 2 cut(s) 251, 353
HinP1I GCGC 2 cut(s) 251, 353
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 4 cut(s) 68, 83, 209, 547
HpaII CCGG 2 cut(s) 63, 168
HphI GGTGA 2 cut(s) 196, 215
Hpy188I TCNGA 2 cut(s) 19, 125
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 4 cut(s) 158, 503, 632, 707
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 428, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 701
HpyF3I CTNAG 3 cut(s) 122, 177, 305
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 251
Hsp92II CATG 1 cut(s) 223
HspAI GCGC 2 cut(s) 251, 353
KasI GGCGCC 1 cut(s) 250
KspI CCGCGG 1 cut(s) 267
Kzo9I GATC 4 cut(s) 6, 10, 402, 687
LmnI GCTCC 5 cut(s) 19, 146, 314, 512, 611
Lsp1109I GCAGC 5 cut(s) 112, 228, 288, 352, 476
LweI GCATC 2 cut(s) 288, 682
MaeI CTAG 2 cut(s) 197, 468
MaeII ACGT 1 cut(s) 153
MalI GATC 4 cut(s) 8, 12, 404, 689
MbiI CCGCTC 2 cut(s) 119, 141
MboI GATC 4 cut(s) 6, 10, 402, 687
MfeI CAATTG 1 cut(s) 485
MluCI AATT 5 cut(s) 325, 397, 485, 494, 501
Mly113I GGCGCC 1 cut(s) 251
MlyI GAGTC 2 cut(s) 77, 541
MnlI CCTC 7 cut(s) 53, 98, 124, 155, 499, 561, 577
MseI TTAA 3 cut(s) 324, 423, 500
MslI CAYNNNNRTG 1 cut(s) 487
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 2 cut(s) 63, 168
MunI CAATTG 1 cut(s) 485
MvnI CGCG 2 cut(s) 266, 348
MwoI GCNNNNNNNGC 1 cut(s) 701
NarI GGCGCC 1 cut(s) 251
NdeII GATC 4 cut(s) 6, 10, 402, 687
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 2 cut(s) 21, 252
NmeAIII GCCGAG 1 cut(s) 82
NspV TTCGAA 1 cut(s) 81
OliI CACNNNNGTG 1 cut(s) 487
PfeI GAWTC 2 cut(s) 83, 209
PleI GAGTC 2 cut(s) 76, 541
PluTI GGCGCC 1 cut(s) 254
PpsI GAGTC 2 cut(s) 76, 541
PspFI CCCAGC 1 cut(s) 246
PspN4I GGNNCC 2 cut(s) 21, 252
RsaI GTAC 1 cut(s) 472
RsaNI GTAC 1 cut(s) 471
RseI CAYNNNNRTG 1 cut(s) 487
SacII CCGCGG 1 cut(s) 267
SaqAI TTAA 3 cut(s) 324, 423, 500
Sau3AI GATC 4 cut(s) 6, 10, 402, 687
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 2 cut(s) 77, 541
SfaNI GCATC 2 cut(s) 288, 682
SfoI GGCGCC 1 cut(s) 252
Sfr303I CCGCGG 1 cut(s) 267
SfuI TTCGAA 1 cut(s) 81
SgrBI CCGCGG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 487
Sse9I AATT 5 cut(s) 325, 397, 485, 494, 501
SspDI GGCGCC 1 cut(s) 250
SspMI CTAG 2 cut(s) 197, 468
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 1 cut(s) 649
TaiI ACGT 1 cut(s) 156
TaqI TCGA 5 cut(s) 9, 81, 446, 658, 690
TasI AATT 5 cut(s) 325, 397, 485, 494, 501
TatI WGTACW 1 cut(s) 470
TauI GCSGC 7 cut(s) 25, 56, 141, 256, 269, 348, 698
TfiI GAWTC 2 cut(s) 83, 209
Tru1I TTAA 3 cut(s) 324, 423, 500
Tru9I TTAA 3 cut(s) 324, 423, 500
TscAI CASTG 1 cut(s) 654
TseI GCWGC 5 cut(s) 100, 216, 301, 365, 464
TspDTI ATGAA 2 cut(s) 248, 252
TspGWI ACGGA 1 cut(s) 624
TspRI CASTG 1 cut(s) 654
XapI RAATTY 2 cut(s) 397, 494
XspI CTAG 2 cut(s) 197, 468
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.