MD13G1065800.v1.1

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013346
Physical Location & Seq
Forward (+)
4557871 .. 4560428
2558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1065800.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGAGATCGATCATCCAGCGCCGTCCGAGCAGAAACTCAAAGCCGAAGACCTCTTCAAAGCCGCCGAGACCGGAGATTCTTCGGCTCTCAAATCTCTCTCTCAGAAACAGCTCGCCGAAGCTTTCTCTCTCAGGAACGAGGACGCCCGCTCCATCCTCCACGTCGCCGTCTCCTCGGGCCGCTCCGAGGTGGTGAAGTTGCTGATTGCTGCCGATGAATCAACGAGTGTGATAAACAGCACGGATGAAGACGGATGGGCGCCGCTTCATTCTGCGGCCAGCATTGGGAATTCGGAAATAGTTGAGCTGTTGCTAAGCAAAGGAGCTGATGTTAATATAAAAAACAATGGCGGTCGATCTGCCCTTCATTATGCTGCCAGTAAGGGATGGTTTGAGATTACTCAGTGTTTGATCTCCCATGGGGCAAATATTAATCTGAAGGACAAGGTTGGTTGCACCCCATTGCACCGGGCAGCGAGCACTGCAAAATCAGAACTGTGTGAGCTTTTAGTTGAGGAAGGAGCAGAAGTTGATGCTGTTGATAAAGCTGGTCAGACTCCTCTCATGGGTGCGGTGATTTGCGATAACAAAGAGGTTTCTCTCCTTCTAATAAGGCACGGAGCAGACGTGGACGTGGAAGACAAGGAAGGATTCACGGTGCTTGGTCGAGCTTCTAAAGAATTTAGACCACTTTTGATCGACGCTGCTAAGGCCATGATTGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

243

Amino Acids

25.71

Weight (kDa)

4.99

Isoelectric Point (pI)

37.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 115 2.2e-17 Ankyrin repeats (3 copies)
Ank_5 PF13857 79 - 125 8.7e-08 Ankyrin repeats (many copies)
Ank PF00023 84 - 116 7.4e-10 Ankyrin repeat
Ank_3 PF13606 84 - 113 4.9e-06 Ankyrin repeat
Ank_4 PF13637 99 - 138 5.4e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 104 - 158 1.1e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 115 - 181 3.3e-14 Ankyrin repeats (3 copies)
Ank PF00023 118 - 149 1.9e-07 Ankyrin repeat
Ank_4 PF13637 122 - 171 4e-09 Ankyrin repeats (many copies)
Ank PF00023 151 - 182 3.4e-06 Ankyrin repeat
Ank_2 PF12796 164 - 227 2e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03430
fragaria_vesca FvH4_4g30470
malus_domestica MD13G1065800.v1.1 MD16G1064400.v1.1
prunus_persica Prupe.1G282400_v2.0.a1
pyrus_communis pycom13g05800 pycom16g05620
rosa_chinensis RchiOBHm_Chr4g0438821
rosa_laevigata RLG00000006328
rosa_multiflora Rmu_sc0002505.1_g000016
rosa_roxburghii Rroxscaffold_5G00379860
rosa_rugosa Rorug04G0313300
rosa_samantha Rh4AG364300 Rh4BG375400 Rh4CG389500 Rh4DG370000
rosa_wichuraiana Rw0G007240 Rw0G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 259
AccBSI CCGCTC 2 cut(s) 150, 183
AciI CCGC 7 cut(s) 63, 148, 181, 263, 275, 351, 572
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 2 cut(s) 289, 680
AcuI CTGAAG 1 cut(s) 458
AcyI GRCGYC 2 cut(s) 144, 260
AfiI CCNNNNNNNGG 3 cut(s) 187, 285, 566
AgsI TTSAA 2 cut(s) 58, 722
AjiI CACGTC 3 cut(s) 163, 628, 634
AluBI AGCT 7 cut(s) 112, 122, 307, 326, 505, 548, 671
AluI AGCT 7 cut(s) 112, 122, 307, 326, 505, 548, 671
Alw21I GWGCWC 1 cut(s) 482
Alw26I GTCTC 2 cut(s) 62, 175
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 3 cut(s) 178, 276, 711
ApeKI GCWGC 4 cut(s) 209, 374, 473, 704
ApoI RAATTY 2 cut(s) 289, 680
AseI ATTAAT 1 cut(s) 432
AspLEI GCGC 2 cut(s) 22, 262
AspS9I GGNCC 1 cut(s) 178
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 2 cut(s) 205, 586
AvaI CYCGRG 1 cut(s) 175
BanI GGYRCC 1 cut(s) 259
BbsI GAAGAC 3 cut(s) 54, 255, 645
Bbv12I GWGCWC 1 cut(s) 482
BbvI GCAGC 4 cut(s) 196, 361, 485, 691
BccI CCATC 3 cut(s) 161, 249, 381
BceAI ACGGC 2 cut(s) 7, 152
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 2 cut(s) 62, 175
BfoI RGCGCY 2 cut(s) 23, 263
BisI GCNGC 8 cut(s) 63, 181, 210, 263, 276, 375, 474, 705
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 8 cut(s) 64, 182, 211, 264, 277, 376, 475, 706
Bme1390I CCNGG 1 cut(s) 470
BmeT110I CYCGRG 1 cut(s) 175
BmgBI CACGTC 3 cut(s) 163, 628, 634
BmgT120I GGNCC 1 cut(s) 178
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 1 cut(s) 470
BmsI GCATC 1 cut(s) 523
BpiI GAAGAC 3 cut(s) 54, 255, 645
Bpu10I CCTNAGC 1 cut(s) 708
Bpu1102I GCTNAGC 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 470
Bsa29I ATCGAT 1 cut(s) 9
BsaHI GRCGYC 2 cut(s) 144, 260
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 174, 186, 418
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 8 cut(s) 134, 164, 315, 345, 513, 543, 612, 642
Bsc4I CCNNNNNNNGG 3 cut(s) 187, 285, 566
Bse1I ACTGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 461
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 3 cut(s) 174, 186, 418
BseGI GGATG 5 cut(s) 13, 153, 250, 260, 392
BseLI CCNNNNNNNGG 3 cut(s) 187, 285, 566
BseMI GCAATG 1 cut(s) 461
BseMII CTCAG 3 cut(s) 116, 145, 416
BseNI ACTGG 1 cut(s) 378
BseRI GAGGAG 2 cut(s) 163, 549
BseXI GCAGC 4 cut(s) 196, 361, 485, 691
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 3 cut(s) 180, 278, 713
BshNI GGYRCC 1 cut(s) 259
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 482
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 2 cut(s) 72, 469
BslI CCNNNNNNNGG 3 cut(s) 187, 285, 566
BsmAI GTCTC 2 cut(s) 62, 175
BsmBI CGTCTC 1 cut(s) 175
BsnI GGCC 3 cut(s) 180, 278, 713
Bso31I GGTCTC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 482
Bsp143I GATC 5 cut(s) 6, 10, 356, 411, 696
Bsp1720I GCTNAGC 1 cut(s) 314
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 7 cut(s) 63, 148, 181, 263, 275, 351, 572
BspANI GGCC 3 cut(s) 180, 278, 713
BspCNI CTCAG 3 cut(s) 115, 144, 415
BspDI ATCGAT 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 2 cut(s) 150, 183
BsrDI GCAATG 1 cut(s) 461
BsrI ACTGG 1 cut(s) 378
BssECI CCNNGG 3 cut(s) 174, 186, 418
BssMI GATC 5 cut(s) 6, 10, 356, 411, 696
BssNI GRCGYC 2 cut(s) 144, 260
BssT1I CCWWGG 1 cut(s) 418
Bst4CI ACNGT 2 cut(s) 498, 658
Bst6I CTCTTC 1 cut(s) 59
BstACI GRCGYC 2 cut(s) 144, 260
BstC8I GCNNGC 4 cut(s) 114, 148, 280, 478
BstDEI CTNAG 5 cut(s) 102, 131, 314, 402, 708
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 5 cut(s) 13, 153, 250, 260, 392
BstH2I RGCGCY 2 cut(s) 23, 263
BstHHI GCGC 2 cut(s) 22, 262
BstKTI GATC 5 cut(s) 9, 13, 359, 414, 699
BstMAI GTCTC 2 cut(s) 62, 175
BstMBI GATC 5 cut(s) 6, 10, 356, 411, 696
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 3 cut(s) 28, 482, 710
BstSCI CCNGG 1 cut(s) 468
BstV1I GCAGC 4 cut(s) 196, 361, 485, 691
BstV2I GAAGAC 3 cut(s) 54, 255, 645
Bsu15I ATCGAT 1 cut(s) 9
BsuRI GGCC 3 cut(s) 180, 278, 713
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 418
BtrI CACGTC 3 cut(s) 163, 628, 634
BtsCI GGATG 5 cut(s) 13, 153, 250, 260, 392
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 410, 480
Cac8I GCNNGC 4 cut(s) 114, 148, 280, 478
CfoI GCGC 2 cut(s) 22, 262
Cfr13I GGNCC 1 cut(s) 178
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 2 cut(s) 152, 710
CviAII CATG 3 cut(s) 419, 565, 715
DdeI CTNAG 5 cut(s) 102, 131, 314, 402, 708
DinI GGCGCC 1 cut(s) 261
DpnI GATC 5 cut(s) 8, 12, 358, 413, 698
DpnII GATC 5 cut(s) 6, 10, 356, 411, 696
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 59
EarI CTCTTC 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 458
Eco88I CYCGRG 1 cut(s) 175
EcoRI GAATTC 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 418
EgeI GGCGCC 1 cut(s) 261
EheI GGCGCC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 418
Esp3I CGTCTC 1 cut(s) 175
FaeI CATG 3 cut(s) 422, 568, 718
FaiI YATR 5 cut(s) 338, 372, 420, 566, 716
FatI CATG 3 cut(s) 418, 564, 714
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 8 cut(s) 63, 181, 210, 263, 276, 375, 474, 705
FokI GGATG 4 cut(s) 140, 257, 267, 399
Fsp4HI GCNGC 8 cut(s) 63, 181, 210, 263, 276, 375, 474, 705
GlaI GCGC 2 cut(s) 21, 261
GluI GCNGC 8 cut(s) 63, 181, 210, 263, 276, 375, 474, 705
HaeII RGCGCY 2 cut(s) 23, 263
HaeIII GGCC 3 cut(s) 180, 278, 713
HapII CCGG 2 cut(s) 72, 469
HgaI GACGC 2 cut(s) 152, 710
HhaI GCGC 2 cut(s) 22, 262
Hin1I GRCGYC 2 cut(s) 144, 260
Hin1II CATG 3 cut(s) 422, 568, 718
Hin6I GCGC 2 cut(s) 20, 260
HinP1I GCGC 2 cut(s) 20, 260
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 4 cut(s) 77, 218, 556, 651
HpaII CCGG 2 cut(s) 72, 469
HphI GGTGA 2 cut(s) 205, 586
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 7 cut(s) 28, 105, 187, 295, 438, 493, 555
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 1 cut(s) 631
Hpy99I CGWCG 2 cut(s) 167, 704
HpyAV CCTTC 6 cut(s) 374, 433, 512, 614, 641, 716
HpyCH4III ACNGT 2 cut(s) 498, 658
HpyCH4IV ACGT 3 cut(s) 162, 627, 633
HpyCH4V TGCA 3 cut(s) 456, 466, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 482, 710
HpyF3I CTNAG 5 cut(s) 102, 131, 314, 402, 708
HpySE526I ACGT 3 cut(s) 162, 627, 633
Hsp92I GRCGYC 2 cut(s) 144, 260
Hsp92II CATG 3 cut(s) 422, 568, 718
HspAI GCGC 2 cut(s) 20, 260
KasI GGCGCC 1 cut(s) 259
Kzo9I GATC 5 cut(s) 6, 10, 356, 411, 696
LmnI GCTCC 5 cut(s) 155, 188, 323, 521, 620
LpnPI CCDG 7 cut(s) 30, 85, 118, 292, 391, 482, 534
Lsp1109I GCAGC 4 cut(s) 196, 361, 485, 691
LweI GCATC 1 cut(s) 523
MaeII ACGT 3 cut(s) 162, 627, 633
MalI GATC 5 cut(s) 8, 12, 358, 413, 698
MbiI CCGCTC 2 cut(s) 150, 183
MboI GATC 5 cut(s) 6, 10, 356, 411, 696
MboII GAAGA 5 cut(s) 46, 59, 72, 260, 650
MhlI GDGCHC 1 cut(s) 482
MluCI AATT 2 cut(s) 289, 680
Mly113I GGCGCC 1 cut(s) 260
MlyI GAGTC 1 cut(s) 550
MnlI CCTC 8 cut(s) 62, 133, 167, 181, 184, 508, 570, 586
MseI TTAA 2 cut(s) 333, 432
MspI CCGG 2 cut(s) 72, 469
MspR9I CCNGG 1 cut(s) 470
MwoI GCNNNNNNNGC 3 cut(s) 28, 482, 710
NarI GGCGCC 1 cut(s) 260
NciI CCSGG 1 cut(s) 470
NcoI CCATGG 1 cut(s) 418
NdeII GATC 5 cut(s) 6, 10, 356, 411, 696
NlaIII CATG 3 cut(s) 422, 568, 718
NlaIV GGNNCC 1 cut(s) 261
NmeAIII GCCGAG 1 cut(s) 91
PcsI WCGNNNNNNNCGW 1 cut(s) 624
PfeI GAWTC 3 cut(s) 77, 218, 651
PkrI GCNGC 8 cut(s) 64, 182, 211, 264, 277, 376, 475, 706
PleI GAGTC 1 cut(s) 550
PluTI GGCGCC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 550
PshBI ATTAAT 1 cut(s) 432
PspN4I GGNNCC 1 cut(s) 261
PspPI GGNCC 1 cut(s) 178
SaqAI TTAA 2 cut(s) 333, 432
SatI GCNGC 8 cut(s) 63, 181, 210, 263, 276, 375, 474, 705
Sau3AI GATC 5 cut(s) 6, 10, 356, 411, 696
Sau96I GGNCC 1 cut(s) 178
SchI GAGTC 1 cut(s) 550
ScrFI CCNGG 1 cut(s) 470
SduI GDGCHC 1 cut(s) 482
SfaNI GCATC 1 cut(s) 523
SfoI GGCGCC 1 cut(s) 261
Sse9I AATT 2 cut(s) 289, 680
SsiI CCGC 7 cut(s) 63, 148, 181, 263, 275, 351, 572
SspDI GGCGCC 1 cut(s) 259
SspI AATATT 1 cut(s) 430
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 2 cut(s) 498, 658
TaiI ACGT 3 cut(s) 165, 630, 636
TaqI TCGA 4 cut(s) 9, 355, 667, 699
TasI AATT 2 cut(s) 289, 680
TauI GCSGC 4 cut(s) 65, 183, 265, 278
TfiI GAWTC 3 cut(s) 77, 218, 651
Tru1I TTAA 2 cut(s) 333, 432
Tru9I TTAA 2 cut(s) 333, 432
TscAI CASTG 2 cut(s) 410, 487
TseI GCWGC 4 cut(s) 209, 374, 473, 704
TspDTI ATGAA 4 cut(s) 231, 257, 261, 356
TspGWI ACGGA 3 cut(s) 257, 267, 633
TspRI CASTG 2 cut(s) 410, 487
VspI ATTAAT 1 cut(s) 432
XapI RAATTY 2 cut(s) 289, 680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.