Rroxscaffold_5G00379860

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013346
Physical Location & Seq
Forward (+)
60094387 .. 60096654
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00379860.1

Sequence Viewer

Length: 720 bp
ATGGAGATCGATCAATCGGAGCCGCAAACCAAAAGCGAAGACCTCTTCAAAGCCGCCGAGACCGGCGACTCTTCGGCGTTCGAATCTCTCACTCCAAAGCAGCTCGCTTCAGCTTCCTCTCTCAGAAACGAGGACGGCCGCTCCCTCCTTCACGTCGCCGTCTCTTCCGGTCAATCTCAGGTGGTAAAGGTAATGCTAGCTGCTGATGAATCGAGTGGTGTGATAAACAGTAGGGATGAAGATGGTTGGGCACCGCTTCATTCTGCGGCGAGCAGTGGGAATGTAGTAATAGTGGAGAAGCTGCTAAGCAAAGGAGCTGATGTTAATTTGAAGAATGATAGTGGTTGCGTTGCACTTCACTATGCTGCCAGCAAAGGATGGATGGAGATTGCTGAAAATTTGATCTCACATGGGGCAAAGCTTAATGTACAGGACAAGGTTGGTTCCACCCCATTGCATCGAGCAGCTAGTACTGGGAAATCACAATTGTGTGAACTTTTAATTGAGGAAGGAGCTGACATTGATGTTGTAGATAAAGCAGGCCAGACTCCTCTTATGGGTGCCGTGATTTGCGAGAACAAGGAGGTTTCTCTCCTTCTAATAAGGCATGGAGCAGATGTGGATGTGGAAGACAAAGAAGGGTACACAGTGCTTGGTCGAGCTTCGAATGAGTTCAGACCATTATTGATTGATGCCGCTAAGGCGATGGTTGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

239

Amino Acids

25.24

Weight (kDa)

4.76

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 112 2e-15 Ankyrin repeats (3 copies)
Ank_5 PF13857 76 - 122 6.4e-07 Ankyrin repeats (many copies)
Ank PF00023 81 - 113 4.5e-08 Ankyrin repeat
Ank_4 PF13637 86 - 132 3.5e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 97 - 178 3.3e-17 Ankyrin repeats (3 copies)
Ank PF00023 115 - 146 3.5e-06 Ankyrin repeat
Ank_4 PF13637 119 - 168 5.9e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 134 - 185 1.3e-07 Ankyrin repeats (many copies)
Ank PF00023 148 - 179 4.1e-07 Ankyrin repeat
Ank_2 PF12796 160 - 222 3.3e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03430
fragaria_vesca FvH4_4g30470
malus_domestica MD13G1065800.v1.1 MD16G1064400.v1.1
prunus_persica Prupe.1G282400_v2.0.a1
pyrus_communis pycom13g05800 pycom16g05620
rosa_chinensis RchiOBHm_Chr4g0438821
rosa_laevigata RLG00000006328
rosa_multiflora Rmu_sc0002505.1_g000016
rosa_roxburghii Rroxscaffold_5G00379860
rosa_rugosa Rorug04G0313300
rosa_samantha Rh4AG364300 Rh4BG375400 Rh4CG389500 Rh4DG370000
rosa_wichuraiana Rw0G007240 Rw0G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 250, 560
AccBSI CCGCTC 1 cut(s) 141
AciI CCGC 6 cut(s) 23, 54, 139, 254, 266, 696
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 3 cut(s) 429, 472, 644
AfiI CCNNNNNNNGG 1 cut(s) 557
AgsI TTSAA 3 cut(s) 49, 331, 713
AjiI CACGTC 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 487
AluBI AGCT 9 cut(s) 103, 113, 200, 301, 317, 421, 467, 515, 662
AluI AGCT 9 cut(s) 103, 113, 200, 301, 317, 421, 467, 515, 662
Alw26I GTCTC 2 cut(s) 53, 166
AoxI GGCC 2 cut(s) 136, 541
ApeKI GCWGC 5 cut(s) 100, 200, 301, 365, 464
ApoI RAATTY 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 671
AsuII TTCGAA 2 cut(s) 81, 665
AsuNHI GCTAGC 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 253
BanI GGYRCC 2 cut(s) 250, 560
BbsI GAAGAC 2 cut(s) 45, 636
BbvI GCAGC 5 cut(s) 112, 187, 288, 352, 476
BccI CCATC 4 cut(s) 236, 372, 376, 700
BceAI ACGGC 3 cut(s) 143, 151, 548
BcoDI GTCTC 2 cut(s) 53, 166
BfaI CTAG 2 cut(s) 197, 468
BglI GCCNNNNNGGC 1 cut(s) 701
BlpI GCTNAGC 1 cut(s) 305
BmcAI AGTACT 1 cut(s) 472
BmgBI CACGTC 1 cut(s) 154
BmiI GGNNCC 4 cut(s) 21, 252, 445, 562
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 466, 682
BmtI GCTAGC 1 cut(s) 200
BmuI ACTGGG 1 cut(s) 483
BpiI GAAGAC 2 cut(s) 45, 636
Bpu10I CCTNAGC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 2 cut(s) 81, 665
Bsa29I ATCGAT 1 cut(s) 9
BsaI GGTCTC 1 cut(s) 53
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 6 cut(s) 125, 155, 306, 336, 603, 633
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 452
BseCI ATCGAT 1 cut(s) 9
BseGI GGATG 4 cut(s) 241, 383, 387, 628
BseLI CCNNNNNNNGG 1 cut(s) 557
BseMI GCAATG 1 cut(s) 452
BseMII CTCAG 2 cut(s) 136, 191
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 540
BseSI GKGCMC 1 cut(s) 253
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 5 cut(s) 112, 187, 288, 352, 476
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 2 cut(s) 138, 543
BshNI GGYRCC 2 cut(s) 250, 560
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 139
BsiSI CCGG 2 cut(s) 63, 168
BslI CCNNNNNNNGG 1 cut(s) 557
BsmAI GTCTC 2 cut(s) 53, 166
BsmBI CGTCTC 1 cut(s) 166
BsnI GGCC 2 cut(s) 138, 543
Bso31I GGTCTC 1 cut(s) 53
Bsp119I TTCGAA 2 cut(s) 81, 665
Bsp1286I GDGCHC 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 427
Bsp143I GATC 3 cut(s) 6, 10, 402
Bsp1720I GCTNAGC 1 cut(s) 305
BspACI CCGC 6 cut(s) 23, 54, 139, 254, 266, 696
BspANI GGCC 2 cut(s) 138, 543
BspCNI CTCAG 2 cut(s) 135, 190
BspDI ATCGAT 1 cut(s) 9
BspLI GGNNCC 4 cut(s) 21, 252, 445, 562
BspOI GCTAGC 1 cut(s) 200
BspT104I TTCGAA 2 cut(s) 81, 665
BspT107I GGYRCC 2 cut(s) 250, 560
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 427
BsrI ACTGG 1 cut(s) 478
BssAI RCCGGY 1 cut(s) 62
BssMI GATC 3 cut(s) 6, 10, 402
Bst4CI ACNGT 2 cut(s) 230, 649
Bst6I CTCTTC 3 cut(s) 50, 76, 169
BstAUI TGTACA 1 cut(s) 427
BstBI TTCGAA 2 cut(s) 81, 665
BstC8I GCNNGC 5 cut(s) 105, 198, 271, 370, 541
BstDEI CTNAG 4 cut(s) 122, 177, 305, 699
BstF5I GGATG 4 cut(s) 241, 383, 387, 628
BstKTI GATC 3 cut(s) 9, 13, 405
BstMAI GTCTC 2 cut(s) 53, 166
BstMBI GATC 3 cut(s) 6, 10, 402
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 701
BstSLI GKGCMC 1 cut(s) 253
BstV1I GCAGC 5 cut(s) 112, 187, 288, 352, 476
BstV2I GAAGAC 2 cut(s) 45, 636
BstZI CGGCCG 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 9
BsuRI GGCC 2 cut(s) 138, 543
BsuTUI ATCGAT 1 cut(s) 9
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 4 cut(s) 241, 383, 387, 628
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 2 cut(s) 280, 654
Cac8I GCNNGC 5 cut(s) 105, 198, 271, 370, 541
Cfr10I RCCGGY 1 cut(s) 62
ClaI ATCGAT 1 cut(s) 9
Csp6I GTAC 3 cut(s) 428, 471, 643
CviAII CATG 2 cut(s) 410, 608
CviQI GTAC 3 cut(s) 428, 471, 643
DdeI CTNAG 4 cut(s) 122, 177, 305, 699
DpnI GATC 3 cut(s) 8, 12, 404
DpnII GATC 3 cut(s) 6, 10, 402
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 3 cut(s) 50, 76, 169
EarI CTCTTC 3 cut(s) 50, 76, 169
EclXI CGGCCG 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 53
Eco52I CGGCCG 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 2 cut(s) 413, 611
FaiI YATR 4 cut(s) 363, 411, 557, 609
FatI CATG 2 cut(s) 409, 607
FokI GGATG 4 cut(s) 248, 390, 394, 635
FspBI CTAG 2 cut(s) 197, 468
HaeIII GGCC 2 cut(s) 138, 543
HapII CCGG 2 cut(s) 63, 168
Hin1II CATG 2 cut(s) 413, 611
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 4 cut(s) 68, 83, 209, 547
HpaII CCGG 2 cut(s) 63, 168
Hpy166II GTNNAC 2 cut(s) 494, 645
Hpy188I TCNGA 3 cut(s) 19, 125, 677
Hpy8I GTNNAC 2 cut(s) 494, 645
Hpy99I CGWCG 1 cut(s) 158
HpyAV CCTTC 5 cut(s) 158, 503, 605, 632, 707
HpyCH4III ACNGT 2 cut(s) 230, 649
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 2 cut(s) 353, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 701
HpyF3I CTNAG 4 cut(s) 122, 177, 305, 699
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 2 cut(s) 413, 611
Kzo9I GATC 3 cut(s) 6, 10, 402
LmnI GCTCC 5 cut(s) 19, 146, 314, 512, 611
LpnPI CCDG 8 cut(s) 76, 164, 181, 382, 416, 459, 525, 557
Lsp1109I GCAGC 5 cut(s) 112, 187, 288, 352, 476
LweI GCATC 2 cut(s) 466, 682
MaeI CTAG 2 cut(s) 197, 468
MaeII ACGT 1 cut(s) 153
MalI GATC 3 cut(s) 8, 12, 404
MbiI CCGCTC 1 cut(s) 141
MboI GATC 3 cut(s) 6, 10, 402
MboII GAAGA 7 cut(s) 37, 50, 63, 156, 251, 343, 641
MfeI CAATTG 1 cut(s) 485
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 4 cut(s) 325, 397, 485, 501
MlyI GAGTC 2 cut(s) 62, 541
MnlI CCTC 7 cut(s) 53, 124, 127, 155, 499, 561, 577
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 3 cut(s) 324, 423, 500
MslI CAYNNNNRTG 1 cut(s) 487
MspI CCGG 2 cut(s) 63, 168
MunI CAATTG 1 cut(s) 485
MwoI GCNNNNNNNGC 1 cut(s) 701
NdeII GATC 3 cut(s) 6, 10, 402
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 2 cut(s) 413, 611
NlaIV GGNNCC 4 cut(s) 21, 252, 445, 562
NmeAIII GCCGAG 1 cut(s) 82
NspV TTCGAA 2 cut(s) 81, 665
OliI CACNNNNGTG 1 cut(s) 487
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 2 cut(s) 83, 209
PleI GAGTC 2 cut(s) 62, 541
PpsI GAGTC 2 cut(s) 62, 541
PspN4I GGNNCC 4 cut(s) 21, 252, 445, 562
RsaI GTAC 3 cut(s) 429, 472, 644
RsaNI GTAC 3 cut(s) 428, 471, 643
RseI CAYNNNNRTG 1 cut(s) 487
SaqAI TTAA 3 cut(s) 324, 423, 500
Sau3AI GATC 3 cut(s) 6, 10, 402
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 2 cut(s) 62, 541
SduI GDGCHC 1 cut(s) 253
SfaNI GCATC 2 cut(s) 466, 682
SfuI TTCGAA 2 cut(s) 81, 665
SmiMI CAYNNNNRTG 1 cut(s) 487
Sse9I AATT 4 cut(s) 325, 397, 485, 501
SsiI CCGC 6 cut(s) 23, 54, 139, 254, 266, 696
SspMI CTAG 2 cut(s) 197, 468
TaaI ACNGT 2 cut(s) 230, 649
TaiI ACGT 1 cut(s) 156
TaqI TCGA 6 cut(s) 9, 81, 212, 460, 658, 665
TasI AATT 4 cut(s) 325, 397, 485, 501
TatI WGTACW 2 cut(s) 427, 470
TauI GCSGC 5 cut(s) 25, 56, 141, 269, 698
TfiI GAWTC 2 cut(s) 83, 209
Tru1I TTAA 3 cut(s) 324, 423, 500
Tru9I TTAA 3 cut(s) 324, 423, 500
TscAI CASTG 2 cut(s) 280, 654
TseI GCWGC 5 cut(s) 100, 200, 301, 365, 464
TspDTI ATGAA 3 cut(s) 222, 248, 252
TspRI CASTG 2 cut(s) 280, 654
XapI RAATTY 1 cut(s) 397
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 2 cut(s) 197, 468
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.