RLG00000006328

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0013346
Physical Location & Seq
Reverse (-)
6268867 .. 6271523
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006328

Sequence Viewer

Length: 1056 bp
ATGGAGATCGATCAATCGGAGCCGCAAATCAAAAGCGAAGACCTCTTCAAAGCCGCCGAGACCGGCGACGCTTCGGCGTTCGAATCTCTCACTCCAAAGCAGCTCGCTTCAGCTTCCTCTCCCAGAAACGAGGACGGCCGCTCCCTCCTTCACGTCGCCGTCTCTTCCGGTCACTCTCAGGTGGTAAAGGTACTGCTAGCTGCTAATGAATCGAGTGGTGTGATAAACAGTACGGATGAAGATGGTTGGGCGCCGCTTCATTCTGCGGCGAGCAGTGGGAATGTAGTAATAGTGGAGATGCTGCTAAGCAAAGGAGCTGATGTTAATTTGAAGAATGATGGTGGTTGCGTTGCACTTCACTATGCTGCCAGCAAAGGATGGATGGAGATTGCTGAAAATTTGATCTCACATGGAGCAAAGCTTAATGTACAAGACAAGGTTGGTTCCACCCCATTGCATCGAGCAGCTAGTACTGGGAAATCACAATTGTGTGAACTTTTAATTGAGGAAGGAGCTGACATTGATGTTTTAGATAAAGCAGGCCAGACTCCTCTTATGAGTGCCGTGATTTGCGAGAACAAGGAGGTTTCTCTCCTTCTAATTAGGCATGGAGCAGATGTGGATGTGGAAGACAAAGAAGGGTACACAGTGCTTGGTCGAGCTTCGAATGAGTTCAGACCGTTATTGATTGATGCCGCTAAGGCGATGCTTGAAGGATTCCAGCAGCCTACTGCAACCTCAAAACGCATGCCAATCTTCTCCTTGGTGCCAGCATGGGGCTTTGACGCCCTTCCTACTACTGGCCGCATTGCTTGTTCAAAACATCTGCCGAATCACCCTCATAACCATTCACCCCGCTGTCTCCCTCGCCAGATTCCTCCCAGCTTTTCCCTCAAATCAACCACATTGAGCTCCATCTCCTTCCTTACAACCCCGCCACCGCCAACTCCAGTGACCCAAAACCTCATATTCAAATCCACTCGTCGCTCTGCTCACCTCCTCGCTGCCCTGTTTTCCTCTGTTTCGCCACCTTCCTCTGTTCTCATCCACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

352

Amino Acids

37.39

Weight (kDa)

5.9

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 112 8.6e-17 Ankyrin repeats (3 copies)
Ank_5 PF13857 76 - 122 1.8e-06 Ankyrin repeats (many copies)
Ank PF00023 81 - 113 1.3e-08 Ankyrin repeat
Ank_4 PF13637 86 - 132 1.8e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 111 - 177 1e-12 Ankyrin repeats (3 copies)
Ank PF00023 115 - 146 7.6e-06 Ankyrin repeat
Ank_4 PF13637 119 - 168 1.1e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 134 - 185 4.1e-07 Ankyrin repeats (many copies)
Ank PF00023 148 - 179 1.4e-06 Ankyrin repeat
Ank_2 PF12796 159 - 224 5e-10 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03430
fragaria_vesca FvH4_4g30470
malus_domestica MD13G1065800.v1.1 MD16G1064400.v1.1
prunus_persica Prupe.1G282400_v2.0.a1
pyrus_communis pycom13g05800 pycom16g05620
rosa_chinensis RchiOBHm_Chr4g0438821
rosa_laevigata RLG00000006328
rosa_multiflora Rmu_sc0002505.1_g000016
rosa_roxburghii Rroxscaffold_5G00379860
rosa_rugosa Rorug04G0313300
rosa_samantha Rh4AG364300 Rh4BG375400 Rh4CG389500 Rh4DG370000
rosa_wichuraiana Rw0G007240 Rw0G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 250, 766
AccBSI CCGCTC 1 cut(s) 141
AcoI YGGCCR 2 cut(s) 136, 802
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 2 cut(s) 251, 786
AfaI GTAC 5 cut(s) 192, 232, 429, 472, 644
AfiI CCNNNNNNNGG 2 cut(s) 776, 800
AgsI TTSAA 5 cut(s) 49, 331, 713, 819, 973
AjiI CACGTC 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 487
Alw21I GWGCWC 1 cut(s) 914
Alw26I GTCTC 3 cut(s) 53, 166, 866
AoxI GGCC 3 cut(s) 136, 541, 802
ApeKI GCWGC 7 cut(s) 100, 200, 301, 365, 464, 724, 1004
ApoI RAATTY 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 253
AsuHPI GGTGA 3 cut(s) 827, 843, 986
AsuII TTCGAA 2 cut(s) 81, 665
AsuNHI GCTAGC 1 cut(s) 196
BanI GGYRCC 2 cut(s) 250, 766
BanII GRGCYC 1 cut(s) 914
BbsI GAAGAC 2 cut(s) 45, 636
Bbv12I GWGCWC 1 cut(s) 914
BbvI GCAGC 7 cut(s) 112, 187, 288, 352, 476, 736, 991
BccI CCATC 5 cut(s) 236, 332, 372, 376, 923
BceAI ACGGC 3 cut(s) 143, 151, 548
BcoDI GTCTC 3 cut(s) 53, 166, 866
BfaI CTAG 2 cut(s) 197, 468
BfoI RGCGCY 1 cut(s) 254
BglI GCCNNNNNGGC 1 cut(s) 701
BlpI GCTNAGC 1 cut(s) 305
BmcAI AGTACT 1 cut(s) 472
BmgBI CACGTC 1 cut(s) 154
BmiI GGNNCC 4 cut(s) 21, 252, 445, 768
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 4 cut(s) 288, 466, 682, 696
BmtI GCTAGC 1 cut(s) 200
BmuI ACTGGG 1 cut(s) 483
BpiI GAAGAC 2 cut(s) 45, 636
BpmI CTGGAG 1 cut(s) 933
Bpu10I CCTNAGC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 2 cut(s) 81, 665
Bsa29I ATCGAT 1 cut(s) 9
BsaHI GRCGYC 2 cut(s) 251, 786
BsaI GGTCTC 1 cut(s) 53
BsaJI CCNNGG 1 cut(s) 762
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 6 cut(s) 125, 155, 306, 336, 603, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 776, 800
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 3 cut(s) 478, 805, 950
Bse3DI GCAATG 2 cut(s) 452, 807
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 5 cut(s) 241, 383, 387, 628, 1044
BseLI CCNNNNNNNGG 2 cut(s) 776, 800
BseMI GCAATG 2 cut(s) 452, 807
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 3 cut(s) 478, 805, 950
BseRI GAGGAG 2 cut(s) 540, 989
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 7 cut(s) 112, 187, 288, 352, 476, 736, 991
BseYI CCCAGC 1 cut(s) 881
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 3 cut(s) 138, 543, 804
BshNI GGYRCC 2 cut(s) 250, 766
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 914
BsiSI CCGG 2 cut(s) 63, 168
BslI CCNNNNNNNGG 2 cut(s) 776, 800
BsmAI GTCTC 3 cut(s) 53, 166, 866
BsmBI CGTCTC 1 cut(s) 166
BsnI GGCC 3 cut(s) 138, 543, 804
Bso31I GGTCTC 1 cut(s) 53
Bsp119I TTCGAA 2 cut(s) 81, 665
Bsp1286I GDGCHC 1 cut(s) 914
Bsp1407I TGTACA 1 cut(s) 427
Bsp143I GATC 3 cut(s) 6, 10, 402
Bsp1720I GCTNAGC 1 cut(s) 305
BspANI GGCC 3 cut(s) 138, 543, 804
BspCNI CTCAG 1 cut(s) 190
BspDI ATCGAT 1 cut(s) 9
BspLI GGNNCC 4 cut(s) 21, 252, 445, 768
BspOI GCTAGC 1 cut(s) 200
BspT104I TTCGAA 2 cut(s) 81, 665
BspT107I GGYRCC 2 cut(s) 250, 766
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 2 cut(s) 452, 807
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 427
BsrI ACTGG 3 cut(s) 478, 805, 950
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 3 cut(s) 6, 10, 402
BssNI GRCGYC 2 cut(s) 251, 786
BssT1I CCWWGG 1 cut(s) 762
Bst4CI ACNGT 3 cut(s) 230, 649, 681
Bst6I CTCTTC 2 cut(s) 50, 169
BstACI GRCGYC 2 cut(s) 251, 786
BstAUI TGTACA 1 cut(s) 427
BstBI TTCGAA 2 cut(s) 81, 665
BstC8I GCNNGC 7 cut(s) 105, 198, 271, 370, 541, 749, 771
BstDEI CTNAG 3 cut(s) 177, 305, 699
BstF5I GGATG 5 cut(s) 241, 383, 387, 628, 1044
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 3 cut(s) 9, 13, 405
BstMAI GTCTC 3 cut(s) 53, 166, 866
BstMBI GATC 3 cut(s) 6, 10, 402
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 701
BstNSI RCATGY 1 cut(s) 751
BstV1I GCAGC 7 cut(s) 112, 187, 288, 352, 476, 736, 991
BstV2I GAAGAC 2 cut(s) 45, 636
BstZI CGGCCG 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 9
BsuRI GGCC 3 cut(s) 138, 543, 804
BsuTUI ATCGAT 1 cut(s) 9
BtgZI GCGATG 1 cut(s) 719
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 5 cut(s) 241, 383, 387, 628, 1044
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 3 cut(s) 280, 654, 957
Cac8I GCNNGC 7 cut(s) 105, 198, 271, 370, 541, 749, 771
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 62
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 2 cut(s) 77, 794
Csp6I GTAC 5 cut(s) 191, 231, 428, 471, 643
CviAII CATG 4 cut(s) 410, 608, 748, 774
CviQI GTAC 5 cut(s) 191, 231, 428, 471, 643
DdeI CTNAG 3 cut(s) 177, 305, 699
DinI GGCGCC 1 cut(s) 252
DpnI GATC 3 cut(s) 8, 12, 404
DpnII GATC 3 cut(s) 6, 10, 402
EaeI YGGCCR 2 cut(s) 136, 802
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 50, 169
EarI CTCTTC 2 cut(s) 50, 169
Ecl136II GAGCTC 1 cut(s) 912
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 1 cut(s) 914
Eco31I GGTCTC 1 cut(s) 53
Eco52I CGGCCG 1 cut(s) 136
Eco53kI GAGCTC 1 cut(s) 912
Eco57I CTGAAG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 912
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 1 cut(s) 914
EgeI GGCGCC 1 cut(s) 252
EheI GGCGCC 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 762
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 4 cut(s) 413, 611, 751, 777
FaiI YATR 8 cut(s) 363, 411, 557, 609, 749, 775, 843, 968
FatI CATG 4 cut(s) 409, 607, 747, 773
FauI CCCGC 2 cut(s) 863, 942
FokI GGATG 5 cut(s) 248, 390, 394, 635, 1031
FriOI GRGCYC 1 cut(s) 914
FspBI CTAG 2 cut(s) 197, 468
GlaI GCGC 1 cut(s) 252
GsaI CCCAGC 1 cut(s) 885
GsuI CTGGAG 1 cut(s) 933
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 3 cut(s) 138, 543, 804
HapII CCGG 2 cut(s) 63, 168
HgaI GACGC 2 cut(s) 77, 794
HhaI GCGC 1 cut(s) 253
Hin1I GRCGYC 2 cut(s) 251, 786
Hin1II CATG 4 cut(s) 413, 611, 751, 777
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 6 cut(s) 83, 209, 547, 717, 832, 874
HpaII CCGG 2 cut(s) 63, 168
HphI GGTGA 3 cut(s) 827, 843, 986
Hpy166II GTNNAC 2 cut(s) 494, 645
Hpy188I TCNGA 2 cut(s) 19, 677
Hpy8I GTNNAC 2 cut(s) 494, 645
Hpy99I CGWCG 3 cut(s) 71, 158, 987
HpyAV CCTTC 8 cut(s) 158, 503, 605, 632, 707, 800, 931, 1041
HpyCH4III ACNGT 3 cut(s) 230, 649, 681
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 3 cut(s) 353, 457, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 701
HpyF3I CTNAG 3 cut(s) 177, 305, 699
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 2 cut(s) 251, 786
Hsp92II CATG 4 cut(s) 413, 611, 751, 777
HspAI GCGC 1 cut(s) 251
KasI GGCGCC 1 cut(s) 250
Kzo9I GATC 3 cut(s) 6, 10, 402
LmnI GCTCC 7 cut(s) 19, 146, 314, 413, 512, 611, 917
Lsp1109I GCAGC 7 cut(s) 112, 187, 288, 352, 476, 736, 991
LweI GCATC 4 cut(s) 288, 466, 682, 696
MaeI CTAG 2 cut(s) 197, 468
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 2 cut(s) 170, 952
MalI GATC 3 cut(s) 8, 12, 404
MbiI CCGCTC 1 cut(s) 141
MboI GATC 3 cut(s) 6, 10, 402
MboII GAAGA 7 cut(s) 37, 50, 156, 251, 343, 641, 748
MfeI CAATTG 1 cut(s) 485
MhlI GDGCHC 1 cut(s) 914
MluCI AATT 5 cut(s) 325, 397, 485, 501, 600
Mly113I GGCGCC 1 cut(s) 251
MlyI GAGTC 1 cut(s) 541
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 3 cut(s) 324, 423, 500
MslI CAYNNNNRTG 1 cut(s) 487
MspA1I CMGCKG 1 cut(s) 858
MspI CCGG 2 cut(s) 63, 168
MunI CAATTG 1 cut(s) 485
MwoI GCNNNNNNNGC 1 cut(s) 701
NarI GGCGCC 1 cut(s) 251
NdeII GATC 3 cut(s) 6, 10, 402
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 4 cut(s) 413, 611, 751, 777
NlaIV GGNNCC 4 cut(s) 21, 252, 445, 768
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 2 cut(s) 170, 952
NspI RCATGY 1 cut(s) 751
NspV TTCGAA 2 cut(s) 81, 665
OliI CACNNNNGTG 1 cut(s) 487
PaeI GCATGC 1 cut(s) 751
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 5 cut(s) 83, 209, 717, 832, 874
PleI GAGTC 1 cut(s) 541
PluTI GGCGCC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 541
Psp124BI GAGCTC 1 cut(s) 914
PspFI CCCAGC 1 cut(s) 881
PspN4I GGNNCC 4 cut(s) 21, 252, 445, 768
RsaI GTAC 5 cut(s) 192, 232, 429, 472, 644
RsaNI GTAC 5 cut(s) 191, 231, 428, 471, 643
RseI CAYNNNNRTG 1 cut(s) 487
SacI GAGCTC 1 cut(s) 914
SaqAI TTAA 3 cut(s) 324, 423, 500
Sau3AI GATC 3 cut(s) 6, 10, 402
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 1 cut(s) 541
SduI GDGCHC 1 cut(s) 914
SfaNI GCATC 4 cut(s) 288, 466, 682, 696
SfoI GGCGCC 1 cut(s) 252
SfuI TTCGAA 2 cut(s) 81, 665
SmiMI CAYNNNNRTG 1 cut(s) 487
SphI GCATGC 1 cut(s) 751
Sse9I AATT 5 cut(s) 325, 397, 485, 501, 600
SspDI GGCGCC 1 cut(s) 250
SspMI CTAG 2 cut(s) 197, 468
SstI GAGCTC 1 cut(s) 914
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 3 cut(s) 230, 649, 681
TaiI ACGT 1 cut(s) 156
TaqI TCGA 6 cut(s) 9, 81, 212, 460, 658, 665
TasI AATT 5 cut(s) 325, 397, 485, 501, 600
TatI WGTACW 2 cut(s) 427, 470
TauI GCSGC 7 cut(s) 25, 56, 141, 256, 269, 698, 807
TfiI GAWTC 5 cut(s) 83, 209, 717, 832, 874
Tru1I TTAA 3 cut(s) 324, 423, 500
Tru9I TTAA 3 cut(s) 324, 423, 500
TscAI CASTG 3 cut(s) 280, 654, 957
TseFI GTSAC 2 cut(s) 170, 952
TseI GCWGC 7 cut(s) 100, 200, 301, 365, 464, 724, 1004
Tsp45I GTSAC 2 cut(s) 170, 952
TspDTI ATGAA 3 cut(s) 222, 248, 252
TspGWI ACGGA 1 cut(s) 248
TspRI CASTG 3 cut(s) 280, 654, 957
XapI RAATTY 1 cut(s) 397
XceI RCATGY 1 cut(s) 751
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 2 cut(s) 197, 468
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.