Rw0G007240

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00270
OG0013346
Physical Location & Seq
Forward (+)
120547 .. 122421
1875 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007240.1

Sequence Viewer

Length: 720 bp
ATGGAGATCGATCAATCGGCGCCGCAAACCAAAAGCGAAGACCTCTTCAAAGCCGCCGAGACCGGCGACGCTTCGGCTTTCGAATCTCTCACTCCAAAGCAGCTCGCTTCAGCTTCCTCTCTCAGAAACGAGGACGGCCGCTCCCTCCTTCACGTCGCCGTCTCTTCCGGTCACTCTCAGGTGGTAAAGGTACTGCTAGCTGCTGATGAATCGAGTGGTGTGATAAACAGTAAGGATGAAGATGGTTGGGCACCGCTGCATTCTGCGGCGAGCAGTGGGAATGTAGTAATAGCGGAGATGCTGCTAAGCAAAGGAGCTGATGTTAATTTGAAGAACGATGGTGGTCGCGTTGCACTTCACTATGCTGCCAGCAAGGGATGGATGGAGATTGCTGAAAATTTGATCTCACATGGGGCGAAGCTTAATGTACAAGACAAGGTTGGTTCCACCCCATTGCATCGAGCAGCTAGTACCGGGAAATCACAATTGTGTGAACTTCTAATTGAGGAAGGAGCTGAAATTGATGTTGTTGATAAAGCAGGCCAGACTCCTCTTATGAGTGCCGTGATTTGCGAGAACAAGGAGGTTTCTCTCCTTCTAATAAGGCATGGAGCAGATGTGGATGTGGAAGACAAAGAAGGATACACAGTGCTTGGTCGAGCTTCGAATGAGTTCAGACCATTATTGATTGATGCCGCTAAGGCGATGCTTGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

239

Amino Acids

25.18

Weight (kDa)

4.88

Isoelectric Point (pI)

33.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 15 - 112 6.7e-17 Ankyrin repeats (3 copies)
Ank PF00023 81 - 113 3.1e-08 Ankyrin repeat
Ank_4 PF13637 86 - 132 1.2e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 105 - 178 2.2e-14 Ankyrin repeats (3 copies)
Ank PF00023 115 - 146 4e-06 Ankyrin repeat
Ank_4 PF13637 119 - 168 5.9e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 134 - 185 1.1e-06 Ankyrin repeats (many copies)
Ank PF00023 148 - 179 3.1e-06 Ankyrin repeat
Ank_2 PF12796 160 - 222 1.3e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03430
fragaria_vesca FvH4_4g30470
malus_domestica MD13G1065800.v1.1 MD16G1064400.v1.1
prunus_persica Prupe.1G282400_v2.0.a1
pyrus_communis pycom13g05800 pycom16g05620
rosa_chinensis RchiOBHm_Chr4g0438821
rosa_laevigata RLG00000006328
rosa_multiflora Rmu_sc0002505.1_g000016
rosa_roxburghii Rroxscaffold_5G00379860
rosa_rugosa Rorug04G0313300
rosa_samantha Rh4AG364300 Rh4BG375400 Rh4CG389500 Rh4DG370000
rosa_wichuraiana Rw0G007240 Rw0G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 19, 250
AccBSI CCGCTC 1 cut(s) 141
AccII CGCG 1 cut(s) 348
AciI CCGC 7 cut(s) 23, 54, 139, 254, 266, 293, 696
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 3 cut(s) 192, 429, 472
AgsI TTSAA 3 cut(s) 49, 331, 713
AjiI CACGTC 1 cut(s) 154
AleI CACNNNNGTG 1 cut(s) 487
AluBI AGCT 8 cut(s) 103, 113, 200, 317, 421, 467, 515, 662
AluI AGCT 8 cut(s) 103, 113, 200, 317, 421, 467, 515, 662
Alw26I GTCTC 2 cut(s) 53, 166
AoxI GGCC 2 cut(s) 136, 541
ApeKI GCWGC 6 cut(s) 100, 200, 256, 301, 365, 464
ApoI RAATTY 1 cut(s) 397
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 1 cut(s) 22
AsuC2I CCSGG 1 cut(s) 475
AsuII TTCGAA 2 cut(s) 81, 665
AsuNHI GCTAGC 1 cut(s) 196
BaeGI GKGCMC 1 cut(s) 253
BanI GGYRCC 2 cut(s) 19, 250
BbsI GAAGAC 2 cut(s) 45, 636
BbvI GCAGC 6 cut(s) 112, 187, 243, 288, 352, 476
BccI CCATC 4 cut(s) 236, 332, 372, 376
BceAI ACGGC 3 cut(s) 143, 151, 548
BciVI GTATCC 1 cut(s) 635
BcnI CCSGG 1 cut(s) 475
BcoDI GTCTC 2 cut(s) 53, 166
BfaI CTAG 2 cut(s) 197, 468
BfoI RGCGCY 1 cut(s) 23
BfuI GTATCC 1 cut(s) 635
BglI GCCNNNNNGGC 1 cut(s) 701
BlpI GCTNAGC 1 cut(s) 305
Bme1390I CCNGG 1 cut(s) 475
BmgBI CACGTC 1 cut(s) 154
BmiI GGNNCC 3 cut(s) 21, 252, 445
BmrFI CCNGG 1 cut(s) 475
BmsI GCATC 4 cut(s) 288, 466, 682, 696
BmtI GCTAGC 1 cut(s) 200
BpiI GAAGAC 2 cut(s) 45, 636
Bpu10I CCTNAGC 1 cut(s) 699
Bpu1102I GCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 2 cut(s) 81, 665
BpuMI CCSGG 1 cut(s) 475
Bsa29I ATCGAT 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 53
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 6 cut(s) 125, 155, 306, 336, 603, 633
Bse118I RCCGGY 1 cut(s) 62
Bse3DI GCAATG 1 cut(s) 452
BseCI ATCGAT 1 cut(s) 9
BseGI GGATG 4 cut(s) 241, 383, 387, 628
BseMI GCAATG 1 cut(s) 452
BseMII CTCAG 2 cut(s) 136, 191
BseRI GAGGAG 1 cut(s) 540
BseSI GKGCMC 1 cut(s) 253
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 6 cut(s) 112, 187, 243, 288, 352, 476
Bsh1236I CGCG 1 cut(s) 348
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 2 cut(s) 138, 543
BshNI GGYRCC 2 cut(s) 19, 250
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 139
BsiSI CCGG 3 cut(s) 63, 168, 474
BsmAI GTCTC 2 cut(s) 53, 166
BsmBI CGTCTC 1 cut(s) 166
BsmI GAATGC 1 cut(s) 259
BsnI GGCC 2 cut(s) 138, 543
Bso31I GGTCTC 1 cut(s) 53
Bsp119I TTCGAA 2 cut(s) 81, 665
Bsp1286I GDGCHC 1 cut(s) 253
Bsp1407I TGTACA 1 cut(s) 427
Bsp143I GATC 3 cut(s) 6, 10, 402
Bsp1720I GCTNAGC 1 cut(s) 305
BspACI CCGC 7 cut(s) 23, 54, 139, 254, 266, 293, 696
BspANI GGCC 2 cut(s) 138, 543
BspCNI CTCAG 2 cut(s) 135, 190
BspDI ATCGAT 1 cut(s) 9
BspFNI CGCG 1 cut(s) 348
BspLI GGNNCC 3 cut(s) 21, 252, 445
BspOI GCTAGC 1 cut(s) 200
BspT104I TTCGAA 2 cut(s) 81, 665
BspT107I GGYRCC 2 cut(s) 19, 250
BspTNI GGTCTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 427
BssAI RCCGGY 1 cut(s) 62
BssMI GATC 3 cut(s) 6, 10, 402
BssNI GRCGYC 1 cut(s) 20
Bst4CI ACNGT 2 cut(s) 230, 649
Bst6I CTCTTC 2 cut(s) 50, 169
BstACI GRCGYC 1 cut(s) 20
BstAUI TGTACA 1 cut(s) 427
BstBI TTCGAA 2 cut(s) 81, 665
BstC8I GCNNGC 5 cut(s) 105, 198, 271, 370, 541
BstDEI CTNAG 4 cut(s) 122, 177, 305, 699
BstF5I GGATG 4 cut(s) 241, 383, 387, 628
BstFNI CGCG 1 cut(s) 348
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 3 cut(s) 9, 13, 405
BstMAI GTCTC 2 cut(s) 53, 166
BstMBI GATC 3 cut(s) 6, 10, 402
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 701
BstSCI CCNGG 1 cut(s) 473
BstSLI GKGCMC 1 cut(s) 253
BstUI CGCG 1 cut(s) 348
BstV1I GCAGC 6 cut(s) 112, 187, 243, 288, 352, 476
BstV2I GAAGAC 2 cut(s) 45, 636
BstZI CGGCCG 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 9
BsuI GTATCC 1 cut(s) 635
BsuRI GGCC 2 cut(s) 138, 543
BsuTUI ATCGAT 1 cut(s) 9
BtrI CACGTC 1 cut(s) 154
BtsCI GGATG 4 cut(s) 241, 383, 387, 628
BtsI GCAGTG 1 cut(s) 280
BtsIMutI CAGTG 2 cut(s) 280, 654
Cac8I GCNNGC 5 cut(s) 105, 198, 271, 370, 541
CfoI GCGC 1 cut(s) 22
Cfr10I RCCGGY 1 cut(s) 62
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 1 cut(s) 77
Csp6I GTAC 3 cut(s) 191, 428, 471
CviAII CATG 2 cut(s) 410, 608
CviQI GTAC 3 cut(s) 191, 428, 471
DdeI CTNAG 4 cut(s) 122, 177, 305, 699
DinI GGCGCC 1 cut(s) 21
DpnI GATC 3 cut(s) 8, 12, 404
DpnII GATC 3 cut(s) 6, 10, 402
EaeI YGGCCR 1 cut(s) 136
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 2 cut(s) 50, 169
EarI CTCTTC 2 cut(s) 50, 169
EclXI CGGCCG 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 53
Eco52I CGGCCG 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 93
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 2 cut(s) 413, 611
FaiI YATR 4 cut(s) 363, 411, 557, 609
FatI CATG 2 cut(s) 409, 607
FokI GGATG 4 cut(s) 248, 390, 394, 635
FspBI CTAG 2 cut(s) 197, 468
GlaI GCGC 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 2 cut(s) 138, 543
HapII CCGG 3 cut(s) 63, 168, 474
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 22
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 2 cut(s) 413, 611
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 3 cut(s) 83, 209, 547
HpaII CCGG 3 cut(s) 63, 168, 474
Hpy166II GTNNAC 1 cut(s) 494
Hpy188I TCNGA 2 cut(s) 125, 677
Hpy8I GTNNAC 1 cut(s) 494
Hpy99I CGWCG 2 cut(s) 71, 158
HpyAV CCTTC 5 cut(s) 158, 503, 605, 632, 707
HpyCH4III ACNGT 2 cut(s) 230, 649
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 3 cut(s) 259, 353, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 701
HpyF3I CTNAG 4 cut(s) 122, 177, 305, 699
HpySE526I ACGT 1 cut(s) 153
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 2 cut(s) 413, 611
HspAI GCGC 1 cut(s) 20
KasI GGCGCC 1 cut(s) 19
Kzo9I GATC 3 cut(s) 6, 10, 402
LmnI GCTCC 4 cut(s) 146, 314, 512, 611
LpnPI CCDG 7 cut(s) 76, 164, 181, 382, 487, 525, 557
Lsp1109I GCAGC 6 cut(s) 112, 187, 243, 288, 352, 476
LweI GCATC 4 cut(s) 288, 466, 682, 696
MaeI CTAG 2 cut(s) 197, 468
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 170
MalI GATC 3 cut(s) 8, 12, 404
MbiI CCGCTC 1 cut(s) 141
MboI GATC 3 cut(s) 6, 10, 402
MboII GAAGA 6 cut(s) 37, 50, 156, 251, 343, 641
MfeI CAATTG 1 cut(s) 485
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 5 cut(s) 325, 397, 485, 501, 519
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 1 cut(s) 541
MnlI CCTC 7 cut(s) 53, 124, 127, 155, 499, 561, 577
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 2 cut(s) 324, 423
MslI CAYNNNNRTG 1 cut(s) 487
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 3 cut(s) 63, 168, 474
MspR9I CCNGG 1 cut(s) 475
MunI CAATTG 1 cut(s) 485
Mva1269I GAATGC 1 cut(s) 259
MvnI CGCG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 701
NarI GGCGCC 1 cut(s) 20
NciI CCSGG 1 cut(s) 475
NdeII GATC 3 cut(s) 6, 10, 402
NheI GCTAGC 1 cut(s) 196
NlaIII CATG 2 cut(s) 413, 611
NlaIV GGNNCC 3 cut(s) 21, 252, 445
NmeAIII GCCGAG 1 cut(s) 82
NmuCI GTSAC 1 cut(s) 170
NspV TTCGAA 2 cut(s) 81, 665
OliI CACNNNNGTG 1 cut(s) 487
PctI GAATGC 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 2 cut(s) 83, 209
PleI GAGTC 1 cut(s) 541
PluTI GGCGCC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 541
PspN4I GGNNCC 3 cut(s) 21, 252, 445
RsaI GTAC 3 cut(s) 192, 429, 472
RsaNI GTAC 3 cut(s) 191, 428, 471
RseI CAYNNNNRTG 1 cut(s) 487
SaqAI TTAA 2 cut(s) 324, 423
Sau3AI GATC 3 cut(s) 6, 10, 402
SchI GAGTC 1 cut(s) 541
ScrFI CCNGG 1 cut(s) 475
SduI GDGCHC 1 cut(s) 253
SfaNI GCATC 4 cut(s) 288, 466, 682, 696
SfoI GGCGCC 1 cut(s) 21
SfuI TTCGAA 2 cut(s) 81, 665
SmiMI CAYNNNNRTG 1 cut(s) 487
Sse9I AATT 5 cut(s) 325, 397, 485, 501, 519
SsiI CCGC 7 cut(s) 23, 54, 139, 254, 266, 293, 696
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 2 cut(s) 197, 468
StyD4I CCNGG 1 cut(s) 473
TaaI ACNGT 2 cut(s) 230, 649
TaiI ACGT 1 cut(s) 156
TaqI TCGA 6 cut(s) 9, 81, 212, 460, 658, 665
TasI AATT 5 cut(s) 325, 397, 485, 501, 519
TatI WGTACW 1 cut(s) 427
TauI GCSGC 5 cut(s) 25, 56, 141, 269, 698
TfiI GAWTC 2 cut(s) 83, 209
Tru1I TTAA 2 cut(s) 324, 423
Tru9I TTAA 2 cut(s) 324, 423
TscAI CASTG 2 cut(s) 280, 654
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 6 cut(s) 100, 200, 256, 301, 365, 464
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 2 cut(s) 222, 252
TspRI CASTG 2 cut(s) 280, 654
XapI RAATTY 1 cut(s) 397
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 2 cut(s) 197, 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.