MD16G1064400.v1.1

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013346
Physical Location & Seq
Forward (+)
4533537 .. 4536895
3359 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1064400.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGAGATCGATCATCCAACGCCGTCCGAGCTGAAACTCAAAGCCGAAGATCTCTTCAAAGCCGCCGAGACCGGTGATTCTTTGGCGTTCAAATCTCTCTCAGTGAAACAGCTCGCCGAAGCTTTCTCTCTCAGGAACGAGGACGCCCGCTCCCTCCTCCACGTCGCTGTCTCTTCAGGTCGCTCCGAGGTGATGAAGCTGCTGATTGATGCTGATGAATCAACGAGTGTGATAAACAGCACGGATGAAGATGGATGGGCGCCGCTTCATTCTGCGGCCAGCATTGGGAATTCAGAAATAGTTGAGCTGTTGCTAAGCAAAGGAGCTGATGTTAATTTAAAAAACAATGGCGGTCGATCTGCCCTTCATTATGCTGCCAGTAAGGGATGGTTTGAGGTTGCTCAGAGTTTGATCTCCCATGGGGCAAAGGTTAATCTGAAGGACAAGGTTGGTTGCACCCCATTGCACCGGGCAGCTAGCACTGCAAAATCACAACTGTGTGAGCTTTTAATTGAGGAAGGGGCAGAGGTTGATGCTATTGATAGAGCTGGTCAGACTCCTCTTATGGGTGCGGTGATTTGCGATAACAAAGAGGTTTGTCTCCTTCTAATAAGGCACGGAGCGGACGTGGACGTGGAAGACAAGGAAGGATACACGGTGCTTGGTCGAGCTTCTAAAGAATTTAGACCACTTTTGATCGACGCTGCTAAGGCCATGATTGAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

243

Amino Acids

25.86

Weight (kDa)

5.05

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 18 - 115 5.3e-16 Ankyrin repeats (3 copies)
Ank_4 PF13637 53 - 105 6.7e-09 Ankyrin repeats (many copies)
Ank_5 PF13857 79 - 125 8.1e-08 Ankyrin repeats (many copies)
Ank PF00023 84 - 116 1.6e-09 Ankyrin repeat
Ank_3 PF13606 84 - 112 7.6e-06 Ankyrin repeat
Ank_4 PF13637 99 - 133 2.9e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 104 - 158 2.9e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 113 - 181 3e-13 Ankyrin repeats (3 copies)
Ank PF00023 118 - 149 8.3e-07 Ankyrin repeat
Ank_4 PF13637 122 - 171 7.4e-10 Ankyrin repeats (many copies)
Ank_2 PF12796 165 - 227 6.7e-09 Ankyrin repeats (3 copies)
Ank PF00023 184 - 215 8.6e-06 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013346)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G03430
fragaria_vesca FvH4_4g30470
malus_domestica MD13G1065800.v1.1 MD16G1064400.v1.1
prunus_persica Prupe.1G282400_v2.0.a1
pyrus_communis pycom13g05800 pycom16g05620
rosa_chinensis RchiOBHm_Chr4g0438821
rosa_laevigata RLG00000006328
rosa_multiflora Rmu_sc0002505.1_g000016
rosa_roxburghii Rroxscaffold_5G00379860
rosa_rugosa Rorug04G0313300
rosa_samantha Rh4AG364300 Rh4BG375400 Rh4CG389500 Rh4DG370000
rosa_wichuraiana Rw0G007240 Rw0G016790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 259
AccBSI CCGCTC 2 cut(s) 150, 623
AciI CCGC 7 cut(s) 63, 148, 263, 275, 351, 572, 623
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 2 cut(s) 289, 680
AcuI CTGAAG 2 cut(s) 159, 458
AcyI GRCGYC 2 cut(s) 144, 260
AfiI CCNNNNNNNGG 2 cut(s) 285, 566
AgeI ACCGGT 1 cut(s) 71
AgsI TTSAA 3 cut(s) 58, 91, 722
AjiI CACGTC 3 cut(s) 163, 628, 634
AleI CACNNNNGTG 1 cut(s) 496
Alw26I GTCTC 3 cut(s) 62, 175, 605
AoxI GGCC 2 cut(s) 276, 711
ApeKI GCWGC 4 cut(s) 199, 374, 473, 704
ApoI RAATTY 2 cut(s) 289, 680
AsiGI ACCGGT 1 cut(s) 71
AspLEI GCGC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 470
AsuHPI GGTGA 3 cut(s) 86, 202, 586
AsuNHI GCTAGC 1 cut(s) 476
BanI GGYRCC 1 cut(s) 259
BbsI GAAGAC 1 cut(s) 645
BbvI GCAGC 4 cut(s) 186, 361, 485, 691
BccI CCATC 3 cut(s) 245, 249, 381
BceAI ACGGC 1 cut(s) 7
BciVI GTATCC 1 cut(s) 644
BcnI CCSGG 1 cut(s) 470
BcoDI GTCTC 3 cut(s) 62, 175, 605
BfaI CTAG 1 cut(s) 477
BfoI RGCGCY 1 cut(s) 263
BfuI GTATCC 1 cut(s) 644
BglII AGATCT 1 cut(s) 49
BisI GCNGC 7 cut(s) 63, 200, 263, 276, 375, 474, 705
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 7 cut(s) 64, 201, 264, 277, 376, 475, 706
Bme1390I CCNGG 1 cut(s) 470
BmgBI CACGTC 3 cut(s) 163, 628, 634
BmiI GGNNCC 1 cut(s) 261
BmrFI CCNGG 1 cut(s) 470
BmsI GCATC 2 cut(s) 199, 523
BmtI GCTAGC 1 cut(s) 480
BpiI GAAGAC 1 cut(s) 645
Bpu10I CCTNAGC 1 cut(s) 708
Bpu1102I GCTNAGC 1 cut(s) 314
BpuMI CCSGG 1 cut(s) 470
Bsa29I ATCGAT 1 cut(s) 9
BsaHI GRCGYC 2 cut(s) 144, 260
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 186, 418
BsaWI WCCGGW 1 cut(s) 71
BsaXI ACNNNNNCTCC 6 cut(s) 134, 164, 315, 345, 612, 642
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 566
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 378
Bse3DI GCAATG 1 cut(s) 461
BseCI ATCGAT 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 186, 418
BseGI GGATG 4 cut(s) 13, 250, 260, 392
BseLI CCNNNNNNNGG 2 cut(s) 285, 566
BseMI GCAATG 1 cut(s) 461
BseMII CTCAG 3 cut(s) 114, 145, 416
BseNI ACTGG 1 cut(s) 378
BseRI GAGGAG 2 cut(s) 146, 549
BseXI GCAGC 4 cut(s) 186, 361, 485, 691
Bsh1285I CGRYCG 1 cut(s) 355
BshFI GGCC 2 cut(s) 278, 713
BshNI GGYRCC 1 cut(s) 259
BshTI ACCGGT 1 cut(s) 71
BshVI ATCGAT 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 355
BsiSI CCGG 2 cut(s) 72, 469
BslI CCNNNNNNNGG 2 cut(s) 285, 566
BsmAI GTCTC 3 cut(s) 62, 175, 605
BsnI GGCC 2 cut(s) 278, 713
Bso31I GGTCTC 1 cut(s) 62
Bsp143I GATC 6 cut(s) 6, 10, 49, 356, 411, 696
Bsp1720I GCTNAGC 1 cut(s) 314
Bsp19I CCATGG 1 cut(s) 418
BspACI CCGC 7 cut(s) 63, 148, 263, 275, 351, 572, 623
BspANI GGCC 2 cut(s) 278, 713
BspCNI CTCAG 3 cut(s) 113, 144, 415
BspDI ATCGAT 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 261
BspOI GCTAGC 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 259
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 2 cut(s) 150, 623
BsrDI GCAATG 1 cut(s) 461
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 378
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 2 cut(s) 186, 418
BssMI GATC 6 cut(s) 6, 10, 49, 356, 411, 696
BssNI GRCGYC 2 cut(s) 144, 260
BssT1I CCWWGG 1 cut(s) 418
Bst4CI ACNGT 2 cut(s) 498, 658
Bst6I CTCTTC 2 cut(s) 59, 178
BstACI GRCGYC 2 cut(s) 144, 260
BstC8I GCNNGC 4 cut(s) 114, 148, 280, 478
BstDEI CTNAG 5 cut(s) 100, 131, 314, 402, 708
BstDSI CCRYGG 1 cut(s) 418
BstF5I GGATG 4 cut(s) 13, 250, 260, 392
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 6 cut(s) 9, 13, 52, 359, 414, 699
BstMAI GTCTC 3 cut(s) 62, 175, 605
BstMBI GATC 6 cut(s) 6, 10, 49, 356, 411, 696
BstMCI CGRYCG 1 cut(s) 355
BstMWI GCNNNNNNNGC 3 cut(s) 28, 482, 710
BstSCI CCNGG 1 cut(s) 468
BstV1I GCAGC 4 cut(s) 186, 361, 485, 691
BstV2I GAAGAC 1 cut(s) 645
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
Bsu15I ATCGAT 1 cut(s) 9
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 2 cut(s) 278, 713
BsuTUI ATCGAT 1 cut(s) 9
BtgI CCRYGG 1 cut(s) 418
BtrI CACGTC 3 cut(s) 163, 628, 634
BtsCI GGATG 4 cut(s) 13, 250, 260, 392
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 2 cut(s) 108, 480
Cac8I GCNNGC 4 cut(s) 114, 148, 280, 478
CfoI GCGC 1 cut(s) 262
Cfr10I RCCGGY 1 cut(s) 71
ClaI ATCGAT 1 cut(s) 9
CseI GACGC 2 cut(s) 152, 710
CspAI ACCGGT 1 cut(s) 71
CviAII CATG 2 cut(s) 419, 715
DdeI CTNAG 5 cut(s) 100, 131, 314, 402, 708
DinI GGCGCC 1 cut(s) 261
DpnI GATC 6 cut(s) 8, 12, 51, 358, 413, 698
DpnII GATC 6 cut(s) 6, 10, 49, 356, 411, 696
DraI TTTAAA 1 cut(s) 339
EaeI YGGCCR 1 cut(s) 276
Eam1104I CTCTTC 2 cut(s) 59, 178
EarI CTCTTC 2 cut(s) 59, 178
Eco130I CCWWGG 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 62
Eco57I CTGAAG 2 cut(s) 159, 458
EcoRI GAATTC 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 418
EgeI GGCGCC 1 cut(s) 261
EheI GGCGCC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 418
FaeI CATG 2 cut(s) 422, 718
FaiI YATR 4 cut(s) 372, 420, 566, 716
FatI CATG 2 cut(s) 418, 714
FauI CCCGC 1 cut(s) 155
Fnu4HI GCNGC 7 cut(s) 63, 200, 263, 276, 375, 474, 705
FokI GGATG 3 cut(s) 257, 267, 399
Fsp4HI GCNGC 7 cut(s) 63, 200, 263, 276, 375, 474, 705
FspBI CTAG 1 cut(s) 477
GlaI GCGC 1 cut(s) 261
GluI GCNGC 7 cut(s) 63, 200, 263, 276, 375, 474, 705
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 2 cut(s) 278, 713
HapII CCGG 2 cut(s) 72, 469
HgaI GACGC 2 cut(s) 152, 710
HhaI GCGC 1 cut(s) 262
Hin1I GRCGYC 2 cut(s) 144, 260
Hin1II CATG 2 cut(s) 422, 718
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HindIII AAGCTT 1 cut(s) 120
HinfI GANTC 3 cut(s) 77, 218, 556
HpaII CCGG 2 cut(s) 72, 469
HphI GGTGA 3 cut(s) 86, 202, 586
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 6 cut(s) 28, 187, 295, 405, 438, 555
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 1 cut(s) 631
Hpy99I CGWCG 2 cut(s) 167, 704
HpyAV CCTTC 6 cut(s) 374, 433, 512, 614, 641, 716
HpyCH4III ACNGT 2 cut(s) 498, 658
HpyCH4IV ACGT 3 cut(s) 162, 627, 633
HpyCH4V TGCA 3 cut(s) 456, 466, 485
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 482, 710
HpyF3I CTNAG 5 cut(s) 100, 131, 314, 402, 708
HpySE526I ACGT 3 cut(s) 162, 627, 633
Hsp92I GRCGYC 2 cut(s) 144, 260
Hsp92II CATG 2 cut(s) 422, 718
HspAI GCGC 1 cut(s) 260
KasI GGCGCC 1 cut(s) 259
Kzo9I GATC 6 cut(s) 6, 10, 49, 356, 411, 696
LmnI GCTCC 4 cut(s) 155, 188, 323, 620
LpnPI CCDG 7 cut(s) 85, 118, 162, 292, 391, 482, 534
Lsp1109I GCAGC 4 cut(s) 186, 361, 485, 691
LweI GCATC 2 cut(s) 199, 523
MaeI CTAG 1 cut(s) 477
MaeII ACGT 3 cut(s) 162, 627, 633
MalI GATC 6 cut(s) 8, 12, 51, 358, 413, 698
MbiI CCGCTC 2 cut(s) 150, 623
MboI GATC 6 cut(s) 6, 10, 49, 356, 411, 696
MboII GAAGA 5 cut(s) 46, 59, 165, 260, 650
MflI RGATCY 1 cut(s) 49
MluCI AATT 4 cut(s) 289, 334, 510, 680
Mly113I GGCGCC 1 cut(s) 260
MlyI GAGTC 1 cut(s) 550
MmeI TCCRAC 1 cut(s) 41
MnlI CCTC 9 cut(s) 133, 164, 167, 181, 388, 508, 520, 570, 586
MseI TTAA 4 cut(s) 333, 338, 432, 509
MslI CAYNNNNRTG 1 cut(s) 496
MspI CCGG 2 cut(s) 72, 469
MspR9I CCNGG 1 cut(s) 470
MwoI GCNNNNNNNGC 3 cut(s) 28, 482, 710
NarI GGCGCC 1 cut(s) 260
NciI CCSGG 1 cut(s) 470
NcoI CCATGG 1 cut(s) 418
NdeII GATC 6 cut(s) 6, 10, 49, 356, 411, 696
NheI GCTAGC 1 cut(s) 476
NlaIII CATG 2 cut(s) 422, 718
NlaIV GGNNCC 1 cut(s) 261
NmeAIII GCCGAG 1 cut(s) 91
OliI CACNNNNGTG 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 624
PfeI GAWTC 2 cut(s) 77, 218
PinAI ACCGGT 1 cut(s) 71
PkrI GCNGC 7 cut(s) 64, 201, 264, 277, 376, 475, 706
PleI GAGTC 1 cut(s) 550
PluTI GGCGCC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 550
PspN4I GGNNCC 1 cut(s) 261
PsuI RGATCY 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 496
SaqAI TTAA 4 cut(s) 333, 338, 432, 509
SatI GCNGC 7 cut(s) 63, 200, 263, 276, 375, 474, 705
Sau3AI GATC 6 cut(s) 6, 10, 49, 356, 411, 696
SchI GAGTC 1 cut(s) 550
ScrFI CCNGG 1 cut(s) 470
SfaNI GCATC 2 cut(s) 199, 523
SfoI GGCGCC 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 496
Sse9I AATT 4 cut(s) 289, 334, 510, 680
SsiI CCGC 7 cut(s) 63, 148, 263, 275, 351, 572, 623
SspDI GGCGCC 1 cut(s) 259
SspMI CTAG 1 cut(s) 477
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 1 cut(s) 418
TaaI ACNGT 2 cut(s) 498, 658
TaiI ACGT 3 cut(s) 165, 630, 636
TaqI TCGA 4 cut(s) 9, 355, 667, 699
TasI AATT 4 cut(s) 289, 334, 510, 680
TauI GCSGC 3 cut(s) 65, 265, 278
TfiI GAWTC 2 cut(s) 77, 218
Tru1I TTAA 4 cut(s) 333, 338, 432, 509
Tru9I TTAA 4 cut(s) 333, 338, 432, 509
TscAI CASTG 2 cut(s) 108, 487
TseI GCWGC 4 cut(s) 199, 374, 473, 704
TspDTI ATGAA 5 cut(s) 209, 231, 257, 261, 356
TspGWI ACGGA 2 cut(s) 257, 633
TspRI CASTG 2 cut(s) 108, 487
XapI RAATTY 2 cut(s) 289, 680
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.