FvH4_4g32280

Protein of unknown function (DUF3326)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004231
Physical Location & Seq
Forward (+)
30995730 .. 31000658
4929 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32280.t4

Sequence Viewer

Length: 1233 bp
ATGACGGTCCTTCTCAGCCGTCCGCTCCCTTCTTTCCCGGCCATCCAACACCGACGTGTCACCAAATCCAGACCGTCCATTTCAACAAGCGTCTATTGCTTCAACTCCAAACACTCCTTCAAGAGAGACTACACAAGCGTGATGATTGTACCTACCGGAGTCGGAGCCGCCATCGGTGGATACGCCGGAGACGCCTTGCCGGTGGCTCGCGCTCTCTCCTCCGTCGTCGATTGCCTCATCACTCATCCCAATGTGCTGAATGCAGCAATGCTGTATTGGCCTATGCCTAATGCTTTGTATGTCGAAGGCTTTGCTCTCGATCGATTTGCCGAAGGCGTATGGGCTCTCAAGCCTGTTCACCAGAACAAGGTCGGGTTGGTTCTTGATTGTGGAATAGAAGAGGAGCTTCGCCTTCGTCATTTGCAAGTGGCTGATGCTGCAAGGGCTTCCCTTGGCTTGCCTGTGGTGGAATATGTTGTCACTGACACTCCTTTGCAGGTAGAAAAGTGGATTGATCCAAAAAGTGGGCAGTCAACAGGGAGGATTAAGCACCCTGAGTCACTACTGAGGGCTGTGCAGGCTTTAATCAAACGCTCACAAGTAAATGCCGTCGCAGTTGTTGGACGTTTCCCGGATGATGATTTTGAAGACACTGATGATTATCGACAAGGGATGGGTATTGATCTATTAGCGGGAGTCGAGGCAGTTATAAGCCATCTAGTAGTGAAAGAATTCCAAATTCCTTGTGCACATGCTCCTGCTTTATTACCCGTACCTCTGAGCCCATCTTTATGCCCAAAATCAGCTGCTGAGGAGTTGGGATACACATTCCTGCCTTGTGTGCTTGCTGGTCTTAGTAATGCACCACAGTACTTGGTCAAGAACTCTGAGTCCGAGCATGGTTGTGTACTGGCAAGTGATGTTGATAGTGTCATCCTGCCTATAGATGCTTGTGCAGGACCTGGTGCTCTTGCTTTTGCACAAAGCAAAGCCAATAAGCCCCTTATAATTGTTGTGGAGGAAAATGAAACAGTCCTTGATGACACACCTGATAAAGTTGGCATTGAAGCAATCAAGGTCTCAAACTATTGGGAAGCAATTGGTGTTATTGCAGCTCACAAGGCTGGAGTAAATCCACAGTCACTACGAAGAAATAGAATTAGCAATCTTCAACGCACTTCTCTGCTGCCTACTAATGGTTATGCCGTTCAAACTGCCTCAACAAATGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3326

Protein Analysis

411

Amino Acids

44.1

Weight (kDa)

6.07

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3326 PF11805 45 - 360 7.2e-147 Protein of unknown function (DUF3326)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 710, 1007
Acc36I ACCTGC 1 cut(s) 487
AccB7I CCANNNNNTGG 1 cut(s) 524
AccBSI CCGCTC 1 cut(s) 25
AccII CGCG 1 cut(s) 210
AciI CCGC 3 cut(s) 23, 168, 692
AclWI GGATC 1 cut(s) 509
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 2 cut(s) 731, 738
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 4 cut(s) 150, 774, 872, 909
AfiI CCNNNNNNNGG 3 cut(s) 367, 524, 1196
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 7 cut(s) 84, 103, 121, 647, 1067, 1172, 1211
AjiI CACGTC 1 cut(s) 56
AjnI CCWGG 1 cut(s) 961
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AleI CACNNNNGTG 2 cut(s) 54, 137
AloI GAACNNNNNNTCC 2 cut(s) 875, 907
AluBI AGCT 3 cut(s) 406, 806, 1115
AluI AGCT 3 cut(s) 406, 806, 1115
Alw21I GWGCWC 2 cut(s) 751, 970
Alw26I GTCTC 3 cut(s) 120, 183, 1084
Alw44I GTGCAC 1 cut(s) 747
AlwI GGATC 1 cut(s) 509
AlwNI CAGNNNCTG 2 cut(s) 809, 962
AoxI GGCC 2 cut(s) 39, 278
ApaLI GTGCAC 1 cut(s) 747
ApeKI GCWGC 5 cut(s) 263, 437, 806, 1112, 1186
ApoI RAATTY 2 cut(s) 731, 738
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 2 cut(s) 7, 959
AsuC2I CCSGG 2 cut(s) 38, 632
AsuHPI GGTGA 2 cut(s) 52, 350
AvaII GGWCC 2 cut(s) 7, 959
BaeGI GKGCMC 1 cut(s) 751
BanII GRGCYC 2 cut(s) 346, 785
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 2 cut(s) 751, 970
BbvCI CCTCAGC 1 cut(s) 810
BbvI GCAGC 5 cut(s) 275, 424, 793, 1124, 1173
BccI CCATC 5 cut(s) 50, 179, 667, 723, 793
BceAI ACGGC 3 cut(s) 3, 593, 1190
BcgI CGANNNNNNTGC 4 cut(s) 293, 308, 327, 342
BciT130I CCWGG 1 cut(s) 963
BciVI GTATCC 2 cut(s) 173, 815
BcnI CCSGG 2 cut(s) 38, 632
BcoDI GTCTC 3 cut(s) 120, 183, 1084
BfaI CTAG 1 cut(s) 719
BfmI CTRYAG 1 cut(s) 942
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 2 cut(s) 173, 815
BisI GCNGC 6 cut(s) 168, 264, 438, 807, 1113, 1187
BlsI GCNGC 6 cut(s) 169, 265, 439, 808, 1114, 1188
BmcAI AGTACT 1 cut(s) 872
Bme1390I CCNGG 3 cut(s) 38, 632, 963
Bme18I GGWCC 2 cut(s) 7, 959
BmgBI CACGTC 1 cut(s) 56
BmgT120I GGNCC 2 cut(s) 7, 959
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 3 cut(s) 38, 632, 963
BmsI GCATC 2 cut(s) 424, 937
BpiI GAAGAC 1 cut(s) 654
BpmI CTGGAG 1 cut(s) 1146
Bpu10I CCTNAGC 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 2 cut(s) 38, 632
Bsa29I ATCGAT 1 cut(s) 322
BsaBI GATNNNNATC 1 cut(s) 660
BsaHI GRCGYC 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 1084
BsaJI CCNNGG 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 524, 1196
Bse118I RCCGGY 1 cut(s) 199
Bse1I ACTGG 1 cut(s) 915
Bse3DI GCAATG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 660
BseBI CCWGG 1 cut(s) 963
BseCI ATCGAT 1 cut(s) 322
BseDI CCNNGG 1 cut(s) 451
BseGI GGATG 5 cut(s) 42, 244, 640, 678, 933
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 3 cut(s) 367, 524, 1196
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 6 cut(s) 28, 546, 557, 770, 801, 879
BseNI ACTGG 1 cut(s) 915
BseRI GAGGAG 3 cut(s) 208, 416, 827
BseSI GKGCMC 1 cut(s) 751
BseXI GCAGC 5 cut(s) 275, 424, 793, 1124, 1173
BsgI GTGCAG 2 cut(s) 596, 975
Bsh1236I CGCG 1 cut(s) 210
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 2 cut(s) 41, 280
BshVI ATCGAT 1 cut(s) 322
BsiEI CGRYCG 1 cut(s) 322
BsiHKAI GWGCWC 2 cut(s) 751, 970
BsiSI CCGG 5 cut(s) 38, 156, 186, 200, 632
BslI CCNNNNNNNGG 3 cut(s) 367, 524, 1196
BsmAI GTCTC 3 cut(s) 120, 183, 1084
BsmBI CGTCTC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 2 cut(s) 41, 280
Bso31I GGTCTC 1 cut(s) 1084
Bsp1286I GDGCHC 4 cut(s) 346, 751, 785, 970
Bsp143I GATC 3 cut(s) 319, 514, 682
BspACI CCGC 3 cut(s) 23, 168, 692
BspANI GGCC 2 cut(s) 41, 280
BspCNI CTCAG 6 cut(s) 27, 547, 558, 771, 802, 880
BspDI ATCGAT 1 cut(s) 322
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 166
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 1084
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 273
BsrFI RCCGGY 1 cut(s) 199
BsrI ACTGG 1 cut(s) 915
BssAI RCCGGY 1 cut(s) 199
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 3 cut(s) 319, 514, 682
BssNI GRCGYC 1 cut(s) 192
BssT1I CCWWGG 1 cut(s) 451
Bst2UI CCWGG 1 cut(s) 963
Bst4CI ACNGT 5 cut(s) 7, 75, 870, 1033, 1140
Bst6I CTCTTC 1 cut(s) 393
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 4 cut(s) 208, 458, 579, 846
BstDEI CTNAG 7 cut(s) 14, 555, 566, 779, 810, 854, 888
BstF5I GGATG 5 cut(s) 42, 244, 640, 678, 933
BstFNI CGCG 1 cut(s) 210
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 3 cut(s) 322, 517, 685
BstMAI GTCTC 3 cut(s) 120, 183, 1084
BstMBI GATC 3 cut(s) 319, 514, 682
BstMCI CGRYCG 1 cut(s) 322
BstMWI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
BstNI CCWGG 1 cut(s) 963
BstNSI RCATGY 1 cut(s) 755
BstSCI CCNGG 3 cut(s) 36, 630, 961
BstSFI CTRYAG 1 cut(s) 942
BstSLI GKGCMC 1 cut(s) 751
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 5 cut(s) 275, 424, 793, 1124, 1173
BstV2I GAAGAC 1 cut(s) 654
Bsu15I ATCGAT 1 cut(s) 322
BsuI GTATCC 2 cut(s) 173, 815
BsuRI GGCC 2 cut(s) 41, 280
BsuTUI ATCGAT 1 cut(s) 322
BtrI CACGTC 1 cut(s) 56
BtsCI GGATG 5 cut(s) 42, 244, 640, 678, 933
BtsIMutI CAGTG 2 cut(s) 480, 651
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 4 cut(s) 208, 458, 579, 846
CaiI CAGNNNCTG 2 cut(s) 809, 962
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 199
Cfr13I GGNCC 2 cut(s) 7, 959
ClaI ATCGAT 1 cut(s) 322
CseI GACGC 2 cut(s) 79, 200
CsiI ACCWGGT 1 cut(s) 961
Csp6I GTAC 4 cut(s) 149, 773, 871, 908
CspCI CAANNNNNGTGG 2 cut(s) 855, 890
CviAII CATG 2 cut(s) 752, 899
CviQI GTAC 4 cut(s) 149, 773, 871, 908
DdeI CTNAG 7 cut(s) 14, 555, 566, 779, 810, 854, 888
DpnI GATC 3 cut(s) 321, 516, 684
DpnII GATC 3 cut(s) 319, 514, 682
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 2 cut(s) 346, 785
Eco31I GGTCTC 1 cut(s) 1084
Eco47I GGWCC 2 cut(s) 7, 959
EcoO109I RGGNCCY 1 cut(s) 959
EcoRI GAATTC 1 cut(s) 731
EcoRII CCWGG 1 cut(s) 961
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 2 cut(s) 346, 785
ErhI CCWWGG 1 cut(s) 451
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 2 cut(s) 755, 902
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FatI CATG 2 cut(s) 751, 898
FauI CCCGC 1 cut(s) 685
Fnu4HI GCNGC 6 cut(s) 168, 264, 438, 807, 1113, 1187
FokI GGATG 5 cut(s) 29, 231, 647, 685, 920
FriOI GRGCYC 2 cut(s) 346, 785
Fsp4HI GCNGC 6 cut(s) 168, 264, 438, 807, 1113, 1187
FspBI CTAG 1 cut(s) 719
GlaI GCGC 1 cut(s) 211
GluI GCNGC 6 cut(s) 168, 264, 438, 807, 1113, 1187
GsuI CTGGAG 1 cut(s) 1146
HaeIII GGCC 2 cut(s) 41, 280
HapII CCGG 5 cut(s) 38, 156, 186, 200, 632
HgaI GACGC 2 cut(s) 79, 200
HhaI GCGC 1 cut(s) 212
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 2 cut(s) 755, 902
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HincII GTYRAC 1 cut(s) 534
HindII GTYRAC 1 cut(s) 534
HinfI GANTC 4 cut(s) 159, 557, 696, 890
HpaII CCGG 5 cut(s) 38, 156, 186, 200, 632
HphI GGTGA 2 cut(s) 52, 350
Hpy166II GTNNAC 4 cut(s) 358, 534, 749, 908
Hpy188I TCNGA 4 cut(s) 164, 780, 889, 895
Hpy188III TCNNGA 5 cut(s) 69, 121, 317, 383, 880
Hpy8I GTNNAC 4 cut(s) 358, 534, 749, 908
Hpy99I CGWCG 4 cut(s) 57, 227, 230, 614
HpyAV CCTTC 6 cut(s) 20, 39, 127, 299, 326, 422
HpyCH4III ACNGT 5 cut(s) 7, 75, 870, 1033, 1140
HpyCH4IV ACGT 2 cut(s) 55, 625
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
HpyF3I CTNAG 7 cut(s) 14, 555, 566, 779, 810, 854, 888
HpySE526I ACGT 2 cut(s) 55, 625
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 2 cut(s) 755, 902
HspAI GCGC 1 cut(s) 210
Kzo9I GATC 3 cut(s) 319, 514, 682
LmnI GCTCC 4 cut(s) 30, 164, 403, 760
Lsp1109I GCAGC 5 cut(s) 275, 424, 793, 1124, 1173
LweI GCATC 2 cut(s) 424, 937
MabI ACCWGGT 1 cut(s) 961
MaeI CTAG 1 cut(s) 719
MaeII ACGT 2 cut(s) 55, 625
MaeIII GTNAC 4 cut(s) 58, 478, 558, 1140
MalI GATC 3 cut(s) 321, 516, 684
MbiI CCGCTC 1 cut(s) 25
MboI GATC 3 cut(s) 319, 514, 682
MboII GAAGA 4 cut(s) 410, 659, 1160, 1161
MfeI CAATTG 1 cut(s) 1098
MhlI GDGCHC 4 cut(s) 346, 751, 785, 970
MluCI AATT 5 cut(s) 731, 738, 1008, 1098, 1158
MlyI GAGTC 4 cut(s) 168, 566, 705, 899
MmeI TCCRAC 3 cut(s) 70, 142, 601
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 2 cut(s) 546, 584
MslI CAYNNNNRTG 5 cut(s) 54, 137, 249, 790, 903
MspA1I CMGCKG 1 cut(s) 806
MspI CCGG 5 cut(s) 38, 156, 186, 200, 632
MspR9I CCNGG 3 cut(s) 38, 632, 963
MunI CAATTG 1 cut(s) 1098
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 1 cut(s) 963
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 8 cut(s) 96, 191, 277, 437, 443, 578, 841, 1121
NciI CCSGG 2 cut(s) 38, 632
NdeII GATC 3 cut(s) 319, 514, 682
NlaIII CATG 2 cut(s) 755, 902
NlaIV GGNNCC 1 cut(s) 166
NmuCI GTSAC 4 cut(s) 58, 478, 558, 1140
NspI RCATGY 1 cut(s) 755
OliI CACNNNNGTG 2 cut(s) 54, 137
PctI GAATGC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 731
PflMI CCANNNNNTGG 1 cut(s) 524
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 6 cut(s) 169, 265, 439, 808, 1114, 1188
Ple19I CGATCG 1 cut(s) 322
PleI GAGTC 4 cut(s) 167, 565, 704, 898
PpsI GAGTC 4 cut(s) 167, 565, 704, 898
PpuMI RGGWCCY 1 cut(s) 959
PsiI TTATAA 2 cut(s) 710, 1007
Psp5II RGGWCCY 1 cut(s) 959
Psp6I CCWGG 1 cut(s) 961
PspGI CCWGG 1 cut(s) 961
PspN4I GGNNCC 1 cut(s) 166
PspPI GGNCC 2 cut(s) 7, 959
PspPPI RGGWCCY 1 cut(s) 959
PstNI CAGNNNCTG 2 cut(s) 809, 962
PvuI CGATCG 1 cut(s) 322
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 4 cut(s) 150, 774, 872, 909
RsaNI GTAC 4 cut(s) 149, 773, 871, 908
RseI CAYNNNNRTG 5 cut(s) 54, 137, 249, 790, 903
SaqAI TTAA 2 cut(s) 546, 584
SatI GCNGC 6 cut(s) 168, 264, 438, 807, 1113, 1187
Sau3AI GATC 3 cut(s) 319, 514, 682
Sau96I GGNCC 2 cut(s) 7, 959
ScaI AGTACT 1 cut(s) 872
SchI GAGTC 4 cut(s) 168, 566, 705, 899
ScrFI CCNGG 3 cut(s) 38, 632, 963
SduI GDGCHC 4 cut(s) 346, 751, 785, 970
SexAI ACCWGGT 1 cut(s) 961
SfaNI GCATC 2 cut(s) 424, 937
SfcI CTRYAG 1 cut(s) 942
SinI GGWCC 2 cut(s) 7, 959
SmiMI CAYNNNNRTG 5 cut(s) 54, 137, 249, 790, 903
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 5 cut(s) 731, 738, 1008, 1098, 1158
SsiI CCGC 3 cut(s) 23, 168, 692
SspMI CTAG 1 cut(s) 719
StyD4I CCNGG 3 cut(s) 36, 630, 961
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 5 cut(s) 7, 75, 870, 1033, 1140
TaiI ACGT 2 cut(s) 58, 628
TaqI TCGA 6 cut(s) 228, 303, 318, 322, 664, 699
TasI AATT 5 cut(s) 731, 738, 1008, 1098, 1158
TatI WGTACW 2 cut(s) 870, 907
TauI GCSGC 1 cut(s) 170
Tru1I TTAA 2 cut(s) 546, 584
Tru9I TTAA 2 cut(s) 546, 584
TscAI CASTG 2 cut(s) 487, 658
TseFI GTSAC 4 cut(s) 58, 478, 558, 1140
TseI GCWGC 5 cut(s) 263, 437, 806, 1112, 1186
Tsp45I GTSAC 4 cut(s) 58, 478, 558, 1140
TspDTI ATGAA 1 cut(s) 1041
TspGWI ACGGA 1 cut(s) 211
TspRI CASTG 2 cut(s) 487, 658
Van91I CCANNNNNTGG 1 cut(s) 524
VneI GTGCAC 1 cut(s) 747
VpaK11BI GGWCC 2 cut(s) 7, 959
XapI RAATTY 2 cut(s) 731, 738
XceI RCATGY 1 cut(s) 755
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 1 cut(s) 719
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.