Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000004

Orthogroup ID

OG0001619

Physical Location & Seq

Genomic Coordinates Reverse (-)

50539500 .. 50547039

Gene Structure 7540 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug04G0331000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2253 bp

ATGGCGGCTGGAAGACATGGGGGTTATCGCGAAAACGAGTTCAGGGAGCGAGAGTCCAGTTTCGAATTGCCGCGGAGGGATTTAGCTTCTGCTAAGGAGGACTTGGAGCGGATGGGGAATGGGAATCGTGATGTTCACAAGGGCCGCGTTCGGGATTTGAGAGATAGAATCAGGGCTAGGTCCAAGGAGAGGGCTGTGGCTAATGGTGGATACCGATCCTCTTCGAGTAGGAGTGATTCCGGCAGCAGTGGCGGTGGCGGGCCTAAGCCGTGTGGGTTTTCTGCCAAGGCATTGGAAAGGGAACCTGGTGAATTGTCAAGTGAGAGCGGGTCCGATGATGCTATTGAATCCGGGTCGCAGGGCCGAGATGATGTAGAGGTTATGAAGGTGGTGGAGAATGGAAGTCAGACTCCCAAGGAGAGGAAAAGGAAGTTTTCGCCTATTGTGTGGGATAGAGATGATAAAGAAGGGAGTAATTCGTCCAAGACTAGGATTACAACAACAGTGGCAACAGCCCTTCCTCCACCCCCACTGGCTAGGGTGTCTCGTCAGTCTCCTAATGTTACTTCAGATAGCAGGGTACAAATATCTTCAGCTAAAATTGATGAAGCCGACAATTTACAACCTTCATCTCCGGTTAAGTCGTCTCCTGTGGCACCTGAGACTGTTAGCAATGGTGTATATGAGTCTCCGGTAGAGTTGTCTTCACCAGATCAGCATTTAGGGGATGTTCGTGAAGCAGAGCAGCTAGAAGAAGAAGATTATGTTCCAACCCGAAATATATCATCTTCAAGATGGGCAGCAGGAAATAATTCTCCTGGTGATGAAGGTGGAATTTCAGAAAACGAGGAAATTCCTAAAAGGAGGAGGAAAATGCCTCGTTTAGGATCAGTGGAAAACAGAGTACGCAACAGCTCATTGACTCCGGAGCTAGGGGAGCTTAAGAAAGATGGGTCTGAACGAGGTAGGGCGAGATCATCCGAGTCTGATGAACGAGATGCCCTTCGAAGGTCTAGTGGGGATGACTATCCTGATAATGACTCAGAGAGGGATGACTACATGGATGTTGACAAGGATAATGGTAATAATGATAGTGTTAGTCGTTATGATACTAGTTCAGAGGATGAGCATGATTCTCCTGAGACACCAGAGCCTGCAGCTCCTCCACAAAGAAGTGTAAACATGCTTCAGGGTTGTAGAAGTGTTGATGAGTTCGAGAGACTAAACAAGATAGATGAGGGCACTTATGGTGTTGTATATCGGGCCCAAGATAAGAAAACTGGTGAAATTGTGGCATTGAAGAAGGTAAAGATGGAGAAGGAAAGAGAAGGTTTTCCATTAACTTCTCTGAGGGAAATAAACATTCTCCTTTCTTTTCATCATCCATCAATTGTCGATGTTAAGGAAGTAGTGGTGGGCAGTAACCTTGATAGCATTTTTATGGTGATGGAATACATGGAGCATGATCTTAAAGCACTCATGGAGACAATGAAGCAGCCATTTAGTCCGAGTGAAGTCAAATGCCTTATGCTCCAGCTATTGGAAGGTGTCAAATATCTTCATGATAATTGGGTGCTTCATCGGGATTTGAAGACATCTAACCTGCTTTTGAATAATCGTGGTGAGTTGAAGATCTGTGACTTTGGGTTGGCTCGTCAATATGGAAGTCCTTTGAAACCGTATACTCATTTGGTGGTTACTCTTTGGTACAGGGCGCCGGAACTTCTGTTGGGAGCAAGAGAATATTCAACGGCAATCGATATGTGGTCACTAGGTTGTATCATGGCTGAGCTGTTATCAAAGGAGCCGCTTTTTAATGGGAAAACTGAATTTGAACAACTTGACAAAATTTTTAAGATACTTGGCACACCAAATGAGACTATTTGGCCTGGGTTTTCCAAGCTTCCTGGAGTAAAGGTCAACTTTGTTAAGCATCAGTATAACCTTTTGCGCAAGAAGTTCCCAGCTACTTCATTTACTGGATCTCCAGTTCTTACTGAAGCTGGATTTGATTTGTTGAATAAGCTTCTAACTTATGACCCTGAGAAGAGAATTTCTGCTGATGCTGCTCTGAATCATGAGTGGTTTCGCGAAGTCCCTCTTCCCAAGACGAAAGATTTCATGCCCACGTTTCCTGCTCAACATGCTCAAGACAGGCGCTCACGGAGAATGTTGAAGAGTCCAGATCCCTTGGAAGAGCAACGGAGAAAGGAGTTGCAAGGGGGTATAGGAACCGGGGGTGTGTTTGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

750

Amino Acids

83.98

Weight (kDa)

5.78

Isoelectric Point (pI)

57.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	405 - 696	2.1e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	408 - 603	1.1e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001619)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g32290 FvH4_5g17111 FvH4_5g17120
malus_domestica	MD06G1064300.v1.1 MD06G1229600.v1.1 MD06G1229700.v1.1 MD13G1026400.v1.1 MD14G1234500.v1.1 MD14G1234600.v1.1 MD16G1029400.v1.1
prunus_persica	Prupe.1G323400_v2.0.a1 Prupe.5G230700_v2.0.a1 Prupe.5G230800_v2.0.a1
pyrus_communis	pycom06g20060 pycom06g20070 pycom13g02490 pycom14g19530 pycom14g19550 pycom16g02530
rosa_chinensis	RchiOBHm_Chr4g0441081 RchiOBHm_Chr7g0178681 RchiOBHm_Chr7g0178691
rosa_laevigata	RLG00000005422 RLG00000005423 RLG00000006118
rosa_multiflora	Rmu_sc0002320.1_g000016 Rmu_sc0002320.1_g000018 Rmu_sc0006317.1_g000008
rosa_roxburghii	Rroxscaffold_3G00274670 Rroxscaffold_3G00274680 Rroxscaffold_5G00382010
rosa_rugosa	Rorug04G0331000 Rorug06G0418700
rosa_samantha	Rh4AG382500 Rh4BG396100 Rh4BG396200 Rh4CG411200 Rh4CG411300 Rh4CG411400 Rh4DG389100 Rh7AG017900 Rh7AG018000 Rh7BG017400 Rh7BG017500 Rh7CG018000 Rh7CG018100
rosa_wichuraiana	Rw4G033010 Rw7G001440 Rw7G001450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1952
Acc36I	ACCTGC	1 cut(s)	1611
AccB1I	GGYRCC	2 cut(s)	655, 1714
AccB7I	CCANNNNNTGG	1 cut(s)	1540
AccBSI	CCGCTC	2 cut(s)	109, 327
AccI	GTMKAC	1 cut(s)	1682
AccII	CGCG	4 cut(s)	30, 73, 147, 2091
AccIII	TCCGGA	1 cut(s)	925
AciI	CCGC	9 cut(s)	5, 71, 73, 109, 145, 252, 258, 327, 1808
AclWI	GGATC	4 cut(s)	210, 895, 1990, 2180
AcsI	RAATTY	5 cut(s)	834, 852, 1829, 1848, 2052
AcuI	CTGAAG	4 cut(s)	552, 576, 1172, 2019
AcyI	GRCGYC	1 cut(s)	1715
AfaI	GTAC	3 cut(s)	582, 906, 1709
AfiI	CCNNNNNNNGG	8 cut(s)	151, 189, 420, 489, 884, 932, 1008, 1540
AflII	CTTAAG	1 cut(s)	941
AhlI	ACTAGT	1 cut(s)	1112
AjnI	CCWGG	4 cut(s)	304, 817, 1888, 1906
AloI	GAACNNNNNNTCC	2 cut(s)	1974, 2006
Alw26I	GTCTC	9 cut(s)	549, 558, 651, 656, 693, 1136, 1213, 1478, 1871
AlwI	GGATC	4 cut(s)	210, 895, 1990, 2180
AlwNI	CAGNNNCTG	1 cut(s)	1154
Aor13HI	TCCGGA	1 cut(s)	925
AoxI	GGCC	5 cut(s)	142, 260, 361, 1263, 1886
ApaI	GGGCCC	1 cut(s)	1267
ApeKI	GCWGC	6 cut(s)	243, 745, 800, 1157, 1495, 2066
ApoI	RAATTY	5 cut(s)	834, 852, 1829, 1848, 2052
Asp700I	GAANNNNTTC	3 cut(s)	811, 1332, 2117
AspLEI	GCGC	3 cut(s)	1717, 1953, 2160
AspS9I	GGNCC	7 cut(s)	142, 180, 260, 330, 361, 1263, 1264
AsuC2I	CCSGG	2 cut(s)	352, 2236
AsuHPI	GGTGA	6 cut(s)	320, 699, 833, 1295, 1456, 1634
AsuII	TTCGAA	2 cut(s)	63, 1006
AvaII	GGWCC	2 cut(s)	180, 330
BaeGI	GKGCMC	2 cut(s)	1244, 1267
BanI	GGYRCC	2 cut(s)	655, 1714
BanII	GRGCYC	1 cut(s)	1267
BarI	GAAGNNNNNNTAC	2 cut(s)	1170, 1202
BbsI	GAAGAC	3 cut(s)	19, 696, 1598
BbvI	GCAGC	6 cut(s)	255, 757, 812, 1169, 1507, 2053
BccI	CCATC	6 cut(s)	106, 789, 944, 1306, 1393, 1441
BceAI	ACGGC	2 cut(s)	253, 1767
BciT130I	CCWGG	4 cut(s)	306, 819, 1890, 1908
BciVI	GTATCC	1 cut(s)	203
BcnI	CCSGG	2 cut(s)	352, 2236
BcoDI	GTCTC	9 cut(s)	549, 558, 651, 656, 693, 1136, 1213, 1478, 1871
BcuI	ACTAGT	1 cut(s)	1112
BfaI	CTAG	9 cut(s)	177, 489, 537, 749, 932, 1014, 1113, 1772, 2251
BfmI	CTRYAG	1 cut(s)	1155
BfoI	RGCGCY	2 cut(s)	1718, 2161
BfrI	CTTAAG	1 cut(s)	941
BfuAI	ACCTGC	1 cut(s)	1611
BfuI	GTATCC	1 cut(s)	203
BglII	AGATCT	1 cut(s)	1632
BlpI	GCTNAGC	1 cut(s)	1788
Bme1390I	CCNGG	6 cut(s)	306, 352, 819, 1890, 1908, 2236
Bme18I	GGWCC	2 cut(s)	180, 330
BmgT120I	GGNCC	7 cut(s)	142, 180, 260, 330, 361, 1263, 1264
BmiI	GGNNCC	7 cut(s)	303, 331, 657, 1265, 1716, 1806, 2233
BmrFI	CCNGG	6 cut(s)	306, 352, 819, 1890, 1908, 2236
BmsI	GCATC	4 cut(s)	328, 988, 1942, 2053
BpiI	GAAGAC	3 cut(s)	19, 696, 1598
BpmI	CTGGAG	3 cut(s)	1517, 1929, 1971
Bpu10I	CCTNAGC	2 cut(s)	93, 264
Bpu1102I	GCTNAGC	1 cut(s)	1788
Bpu14I	TTCGAA	2 cut(s)	63, 1006
BpuEI	CTTGAG	1 cut(s)	2133
BpuMI	CCSGG	2 cut(s)	352, 2236
Bsa29I	ATCGAT	1 cut(s)	1758
BsaBI	GATNNNNATC	2 cut(s)	214, 1026
BsaHI	GRCGYC	1 cut(s)	1715
BsaJI	CCNNGG	7 cut(s)	71, 183, 285, 414, 1889, 2190, 2235
BsaWI	WCCGGW	3 cut(s)	634, 691, 925
BsaXI	ACNNNNNCTCC	6 cut(s)	38, 68, 1902, 1932, 1969, 1999
Bsc4I	CCNNNNNNNGG	8 cut(s)	151, 189, 420, 489, 884, 932, 1008, 1540
Bse1I	ACTGG	5 cut(s)	57, 537, 1285, 1984, 1988
Bse3DI	GCAATG	1 cut(s)	679
Bse8I	GATNNNNATC	2 cut(s)	214, 1026
BseAI	TCCGGA	1 cut(s)	925
BseBI	CCWGG	4 cut(s)	306, 819, 1890, 1908
BseCI	ATCGAT	1 cut(s)	1758
BseDI	CCNNGG	7 cut(s)	71, 183, 285, 414, 1889, 2190, 2235
BseGI	GGATG	8 cut(s)	117, 733, 977, 1027, 1057, 1069, 1129, 1381
BseJI	GATNNNNATC	2 cut(s)	214, 1026
BseLI	CCNNNNNNNGG	8 cut(s)	151, 189, 420, 489, 884, 932, 1008, 1540
BseMI	GCAATG	1 cut(s)	679
BseMII	CTCAG	6 cut(s)	651, 1056, 1131, 1340, 1779, 2034
BseNI	ACTGG	5 cut(s)	57, 537, 1285, 1984, 1988
BseRI	GAGGAG	2 cut(s)	880, 1152
BseSI	GKGCMC	2 cut(s)	1244, 1267
BseXI	GCAGC	6 cut(s)	255, 757, 812, 1169, 1507, 2053
BseYI	CCCAGC	1 cut(s)	1963
Bsh1236I	CGCG	4 cut(s)	30, 73, 147, 2091
BshFI	GGCC	5 cut(s)	144, 262, 363, 1265, 1888
BshNI	GGYRCC	2 cut(s)	655, 1714
BshVI	ATCGAT	1 cut(s)	1758
BsiSI	CCGG	7 cut(s)	240, 351, 635, 692, 926, 1718, 2235
BslFI	GGGAC	1 cut(s)	2081
BslI	CCNNNNNNNGG	8 cut(s)	151, 189, 420, 489, 884, 932, 1008, 1540
BsmAI	GTCTC	9 cut(s)	549, 558, 651, 656, 693, 1136, 1213, 1478, 1871
BsmBI	CGTCTC	1 cut(s)	651
BsmFI	GGGAC	1 cut(s)	2081
BsnI	GGCC	5 cut(s)	144, 262, 363, 1265, 1888
Bsp119I	TTCGAA	2 cut(s)	63, 1006
Bsp120I	GGGCCC	1 cut(s)	1263
Bsp1286I	GDGCHC	2 cut(s)	1244, 1267
Bsp13I	TCCGGA	1 cut(s)	925
Bsp143I	GATC	8 cut(s)	215, 712, 887, 974, 1465, 1632, 1982, 2185
Bsp1720I	GCTNAGC	1 cut(s)	1788
Bsp68I	TCGCGA	2 cut(s)	30, 2091
BspACI	CCGC	9 cut(s)	5, 71, 73, 109, 145, 252, 258, 327, 1808
BspANI	GGCC	5 cut(s)	144, 262, 363, 1265, 1888
BspCNI	CTCAG	6 cut(s)	652, 1055, 1132, 1341, 1780, 2035
BspDI	ATCGAT	1 cut(s)	1758
BspEI	TCCGGA	1 cut(s)	925
BspFNI	CGCG	4 cut(s)	30, 73, 147, 2091
BspHI	TCATGA	2 cut(s)	1561, 2077
BspLI	GGNNCC	7 cut(s)	303, 331, 657, 1265, 1716, 1806, 2233
BspMAI	CTGCAG	1 cut(s)	1159
BspMI	ACCTGC	1 cut(s)	1611
BspPI	GGATC	4 cut(s)	210, 895, 1990, 2180
BspQI	GCTCTTC	1 cut(s)	2190
BspT104I	TTCGAA	2 cut(s)	63, 1006
BspT107I	GGYRCC	2 cut(s)	655, 1714
BspTI	CTTAAG	1 cut(s)	941
BsrBI	CCGCTC	2 cut(s)	109, 327
BsrDI	GCAATG	1 cut(s)	679
BsrI	ACTGG	5 cut(s)	57, 537, 1285, 1984, 1988
BssECI	CCNNGG	7 cut(s)	71, 183, 285, 414, 1889, 2190, 2235
BssMI	GATC	8 cut(s)	215, 712, 887, 974, 1465, 1632, 1982, 2185
BssNAI	GTATAC	1 cut(s)	1683
BssNI	GRCGYC	1 cut(s)	1715
BssT1I	CCWWGG	4 cut(s)	183, 285, 414, 2190
Bst1107I	GTATAC	1 cut(s)	1683
Bst2UI	CCWGG	4 cut(s)	306, 819, 1890, 1908
Bst4CI	ACNGT	3 cut(s)	505, 667, 1680
Bst6I	CTCTTC	5 cut(s)	226, 2042, 2106, 2171, 2190
BstACI	GRCGYC	1 cut(s)	1715
BstAFI	CTTAAG	1 cut(s)	941
BstBI	TTCGAA	2 cut(s)	63, 1006
BstC8I	GCNNGC	2 cut(s)	260, 1155
BstDEI	CTNAG	8 cut(s)	93, 264, 660, 1042, 1140, 1349, 1788, 2043
BstDSI	CCRYGG	1 cut(s)	71
BstENI	CCTNNNNNAGG	1 cut(s)	882
BstF5I	GGATG	8 cut(s)	117, 733, 977, 1027, 1057, 1069, 1129, 1381
BstFNI	CGCG	4 cut(s)	30, 73, 147, 2091
BstH2I	RGCGCY	2 cut(s)	1718, 2161
BstHHI	GCGC	3 cut(s)	1717, 1953, 2160
BstKTI	GATC	8 cut(s)	218, 715, 890, 977, 1468, 1635, 1985, 2188
BstMAI	GTCTC	9 cut(s)	549, 558, 651, 656, 693, 1136, 1213, 1478, 1871
BstMBI	GATC	8 cut(s)	215, 712, 887, 974, 1465, 1632, 1982, 2185
BstMWI	GCNNNNNNNGC	4 cut(s)	249, 937, 2066, 2144
BstNI	CCWGG	4 cut(s)	306, 819, 1890, 1908
BstNSI	RCATGY	2 cut(s)	1186, 2147
BstSCI	CCNGG	6 cut(s)	304, 350, 817, 1888, 1906, 2234
BstSFI	CTRYAG	1 cut(s)	1155
BstSLI	GKGCMC	2 cut(s)	1244, 1267
BstUI	CGCG	4 cut(s)	30, 73, 147, 2091
BstV1I	GCAGC	6 cut(s)	255, 757, 812, 1169, 1507, 2053
BstV2I	GAAGAC	3 cut(s)	19, 696, 1598
BstX2I	RGATCY	3 cut(s)	1632, 1982, 2185
BstXI	CCANNNNNNTGG	1 cut(s)	292
BstYI	RGATCY	3 cut(s)	1632, 1982, 2185
BstZ17I	GTATAC	1 cut(s)	1683
Bsu15I	ATCGAT	1 cut(s)	1758
BsuI	GTATCC	1 cut(s)	203
BsuRI	GGCC	5 cut(s)	144, 262, 363, 1265, 1888
BsuTUI	ATCGAT	1 cut(s)	1758
BtgI	CCRYGG	1 cut(s)	71
BtsCI	GGATG	8 cut(s)	117, 733, 977, 1027, 1057, 1069, 1129, 1381
BtsI	GCAGTG	1 cut(s)	253
BtsIMutI	CAGTG	4 cut(s)	253, 510, 530, 897
BtuMI	TCGCGA	2 cut(s)	30, 2091
BveI	ACCTGC	1 cut(s)	1611
Cac8I	GCNNGC	2 cut(s)	260, 1155
CaiI	CAGNNNCTG	1 cut(s)	1154
CciI	TCATGA	2 cut(s)	1561, 2077
CfoI	GCGC	3 cut(s)	1717, 1953, 2160
Cfr13I	GGNCC	7 cut(s)	142, 180, 260, 330, 361, 1263, 1264
Cfr42I	CCGCGG	1 cut(s)	74
ClaI	ATCGAT	1 cut(s)	1758
CsiI	ACCWGGT	1 cut(s)	304
Csp6I	GTAC	3 cut(s)	581, 905, 1708
CspCI	CAANNNNNGTGG	2 cut(s)	486, 521
CviQI	GTAC	3 cut(s)	581, 905, 1708
DdeI	CTNAG	8 cut(s)	93, 264, 660, 1042, 1140, 1349, 1788, 2043
DinI	GGCGCC	1 cut(s)	1716
DpnI	GATC	8 cut(s)	217, 714, 889, 976, 1467, 1634, 1984, 2187
DpnII	GATC	8 cut(s)	215, 712, 887, 974, 1465, 1632, 1982, 2185
Eam1104I	CTCTTC	5 cut(s)	226, 2042, 2106, 2171, 2190
EarI	CTCTTC	5 cut(s)	226, 2042, 2106, 2171, 2190
Eco130I	CCWWGG	4 cut(s)	183, 285, 414, 2190
Eco24I	GRGCYC	1 cut(s)	1267
Eco47I	GGWCC	2 cut(s)	180, 330
Eco57I	CTGAAG	4 cut(s)	552, 576, 1172, 2019
EcoNI	CCTNNNNNAGG	1 cut(s)	882
EcoRII	CCWGG	4 cut(s)	304, 817, 1888, 1906
EcoT14I	CCWWGG	4 cut(s)	183, 285, 414, 2190
EcoT38I	GRGCYC	1 cut(s)	1267
EgeI	GGCGCC	1 cut(s)	1716
EheI	GGCGCC	1 cut(s)	1716
ErhI	CCWWGG	4 cut(s)	183, 285, 414, 2190
Esp3I	CGTCTC	1 cut(s)	651
FalI	AAGNNNNNCTT	6 cut(s)	86, 118, 1907, 1939, 2085, 2117
FaqI	GGGAC	1 cut(s)	2081
FauI	CCCGC	2 cut(s)	251, 320
FblI	GTMKAC	1 cut(s)	1682
FokI	GGATG	8 cut(s)	124, 740, 964, 1034, 1064, 1076, 1136, 1368
FriOI	GRGCYC	1 cut(s)	1267
FspBI	CTAG	9 cut(s)	177, 489, 537, 749, 932, 1014, 1113, 1772, 2251
FspI	TGCGCA	1 cut(s)	1952
GlaI	GCGC	3 cut(s)	1716, 1952, 2159
GsaI	CCCAGC	1 cut(s)	1967
GsuI	CTGGAG	3 cut(s)	1517, 1929, 1971
HaeII	RGCGCY	2 cut(s)	1718, 2161
HaeIII	GGCC	5 cut(s)	144, 262, 363, 1265, 1888
HapII	CCGG	7 cut(s)	240, 351, 635, 692, 926, 1718, 2235
HhaI	GCGC	3 cut(s)	1717, 1953, 2160
Hin1I	GRCGYC	1 cut(s)	1715
Hin6I	GCGC	3 cut(s)	1715, 1951, 2158
HinP1I	GCGC	3 cut(s)	1715, 1951, 2158
HincII	GTYRAC	2 cut(s)	1069, 1921
HindII	GTYRAC	2 cut(s)	1069, 1921
HindIII	AAGCTT	2 cut(s)	1901, 2024
HpaII	CCGG	7 cut(s)	240, 351, 635, 692, 926, 1718, 2235
HphI	GGTGA	6 cut(s)	320, 699, 833, 1295, 1456, 1634
Hpy166II	GTNNAC	5 cut(s)	136, 1069, 1180, 1683, 1921
Hpy8I	GTNNAC	5 cut(s)	136, 1069, 1180, 1683, 1921
HpyCH4III	ACNGT	3 cut(s)	505, 667, 1680
HpyCH4IV	ACGT	1 cut(s)	2129
HpyCH4V	TGCA	2 cut(s)	1157, 2218
HpyF10VI	GCNNNNNNNGC	4 cut(s)	249, 937, 2066, 2144
HpyF3I	CTNAG	8 cut(s)	93, 264, 660, 1042, 1140, 1349, 1788, 2043
HpySE526I	ACGT	1 cut(s)	2129
Hsp92I	GRCGYC	1 cut(s)	1715
HspAI	GCGC	3 cut(s)	1715, 1951, 2158
KasI	GGCGCC	1 cut(s)	1714
Kpn2I	TCCGGA	1 cut(s)	925
KspI	CCGCGG	1 cut(s)	74
Kzo9I	GATC	8 cut(s)	215, 712, 887, 974, 1465, 1632, 1982, 2185
LguI	GCTCTTC	1 cut(s)	2190
LmnI	GCTCC	9 cut(s)	46, 106, 928, 937, 1165, 1459, 1536, 1733, 1804
Lsp1109I	GCAGC	6 cut(s)	255, 757, 812, 1169, 1507, 2053
LweI	GCATC	4 cut(s)	328, 988, 1942, 2053
MabI	ACCWGGT	1 cut(s)	304
MaeI	CTAG	9 cut(s)	177, 489, 537, 749, 932, 1014, 1113, 1772, 2251
MaeII	ACGT	1 cut(s)	2129
MaeIII	GTNAC	5 cut(s)	562, 1421, 1637, 1696, 1767
MalI	GATC	8 cut(s)	217, 714, 889, 976, 1467, 1634, 1984, 2187
MbiI	CCGCTC	2 cut(s)	109, 327
MboI	GATC	8 cut(s)	215, 712, 887, 974, 1465, 1632, 1982, 2185
MfeI	CAATTG	1 cut(s)	1389
MflI	RGATCY	3 cut(s)	1632, 1982, 2185
MhlI	GDGCHC	2 cut(s)	1244, 1267
Mly113I	GGCGCC	1 cut(s)	1715
MlyI	GAGTC	7 cut(s)	62, 403, 695, 916, 992, 1034, 2188
MmeI	TCCRAC	1 cut(s)	794
MroI	TCCGGA	1 cut(s)	925
MroXI	GAANNNNTTC	3 cut(s)	811, 1332, 2117
MseI	TTAA	8 cut(s)	639, 942, 1340, 1401, 1470, 1815, 1854, 1929
MslI	CAYNNNNRTG	1 cut(s)	1439
MspA1I	CMGCKG	1 cut(s)	73
MspCI	CTTAAG	1 cut(s)	941
MspI	CCGG	7 cut(s)	240, 351, 635, 692, 926, 1718, 2235
MspR9I	CCNGG	6 cut(s)	306, 352, 819, 1890, 1908, 2236
MunI	CAATTG	1 cut(s)	1389
MvaI	CCWGG	4 cut(s)	306, 819, 1890, 1908
MvnI	CGCG	4 cut(s)	30, 73, 147, 2091
MwoI	GCNNNNNNNGC	4 cut(s)	249, 937, 2066, 2144
NarI	GGCGCC	1 cut(s)	1715
NciI	CCSGG	2 cut(s)	352, 2236
NdeII	GATC	8 cut(s)	215, 712, 887, 974, 1465, 1632, 1982, 2185
NlaIV	GGNNCC	7 cut(s)	303, 331, 657, 1265, 1716, 1806, 2233
NmeAIII	GCCGAG	1 cut(s)	389
NmuCI	GTSAC	2 cut(s)	1637, 1767
NruI	TCGCGA	2 cut(s)	30, 2091
NsbI	TGCGCA	1 cut(s)	1952
NspI	RCATGY	2 cut(s)	1186, 2147
NspV	TTCGAA	2 cut(s)	63, 1006
PagI	TCATGA	2 cut(s)	1561, 2077
PciSI	GCTCTTC	1 cut(s)	2190
PdmI	GAANNNNTTC	3 cut(s)	811, 1332, 2117
PfeI	GAWTC	6 cut(s)	124, 168, 236, 347, 1133, 2074
PflMI	CCANNNNNTGG	1 cut(s)	1540
PfoI	TCCNGGA	1 cut(s)	1906
PleI	GAGTC	7 cut(s)	61, 403, 694, 916, 991, 1034, 2187
PluTI	GGCGCC	1 cut(s)	1718
PpsI	GAGTC	7 cut(s)	61, 403, 694, 916, 991, 1034, 2187
Psp6I	CCWGG	4 cut(s)	304, 817, 1888, 1906
PspFI	CCCAGC	1 cut(s)	1963
PspGI	CCWGG	4 cut(s)	304, 817, 1888, 1906
PspN4I	GGNNCC	7 cut(s)	303, 331, 657, 1265, 1716, 1806, 2233
PspOMI	GGGCCC	1 cut(s)	1263
PspPI	GGNCC	7 cut(s)	142, 180, 260, 330, 361, 1263, 1264
PstI	CTGCAG	1 cut(s)	1159
PstNI	CAGNNNCTG	1 cut(s)	1154
PsuI	RGATCY	3 cut(s)	1632, 1982, 2185
RruI	TCGCGA	2 cut(s)	30, 2091
RsaI	GTAC	3 cut(s)	582, 906, 1709
RsaNI	GTAC	3 cut(s)	581, 905, 1708
RseI	CAYNNNNRTG	1 cut(s)	1439
SacII	CCGCGG	1 cut(s)	74
SapI	GCTCTTC	1 cut(s)	2190
SaqAI	TTAA	8 cut(s)	639, 942, 1340, 1401, 1470, 1815, 1854, 1929
Sau3AI	GATC	8 cut(s)	215, 712, 887, 974, 1465, 1632, 1982, 2185
Sau96I	GGNCC	7 cut(s)	142, 180, 260, 330, 361, 1263, 1264
SchI	GAGTC	7 cut(s)	62, 403, 695, 916, 992, 1034, 2188
ScrFI	CCNGG	6 cut(s)	306, 352, 819, 1890, 1908, 2236
SduI	GDGCHC	2 cut(s)	1244, 1267
SexAI	ACCWGGT	1 cut(s)	304
SfaNI	GCATC	4 cut(s)	328, 988, 1942, 2053
SfcI	CTRYAG	1 cut(s)	1155
SfoI	GGCGCC	1 cut(s)	1716
Sfr303I	CCGCGG	1 cut(s)	74
SfuI	TTCGAA	2 cut(s)	63, 1006
SgrBI	CCGCGG	1 cut(s)	74
SinI	GGWCC	2 cut(s)	180, 330
SmiMI	CAYNNNNRTG	1 cut(s)	1439
SmlI	CTYRAG	2 cut(s)	941, 2148
SmoI	CTYRAG	2 cut(s)	941, 2148
SpeI	ACTAGT	1 cut(s)	1112
SsiI	CCGC	9 cut(s)	5, 71, 73, 109, 145, 252, 258, 327, 1808
SspDI	GGCGCC	1 cut(s)	1714
SspI	AATATT	1 cut(s)	1745
SspMI	CTAG	9 cut(s)	177, 489, 537, 749, 932, 1014, 1113, 1772, 2251
StyD4I	CCNGG	6 cut(s)	304, 350, 817, 1888, 1906, 2234
StyI	CCWWGG	4 cut(s)	183, 285, 414, 2190
TaaI	ACNGT	3 cut(s)	505, 667, 1680
TaiI	ACGT	1 cut(s)	2132
TaqI	TCGA	6 cut(s)	63, 224, 1006, 1215, 1395, 1758
TauI	GCSGC	4 cut(s)	8, 73, 147, 1810
TfiI	GAWTC	6 cut(s)	124, 168, 236, 347, 1133, 2074
Tru1I	TTAA	8 cut(s)	639, 942, 1340, 1401, 1470, 1815, 1854, 1929
Tru9I	TTAA	8 cut(s)	639, 942, 1340, 1401, 1470, 1815, 1854, 1929
TscAI	CASTG	4 cut(s)	253, 510, 537, 897
TseFI	GTSAC	2 cut(s)	1637, 1767
TseI	GCWGC	6 cut(s)	243, 745, 800, 1157, 1495, 2066
Tsp45I	GTSAC	2 cut(s)	1637, 1767
TspGWI	ACGGA	2 cut(s)	2179, 2218
TspRI	CASTG	4 cut(s)	253, 510, 537, 897
Van91I	CCANNNNNTGG	1 cut(s)	1540
Vha464I	CTTAAG	1 cut(s)	941
VpaK11BI	GGWCC	2 cut(s)	180, 330
XagI	CCTNNNNNAGG	1 cut(s)	882
XapI	RAATTY	5 cut(s)	834, 852, 1829, 1848, 2052
XceI	RCATGY	2 cut(s)	1186, 2147
XmiI	GTMKAC	1 cut(s)	1682
XmnI	GAANNNNTTC	3 cut(s)	811, 1332, 2117
XspI	CTAG	9 cut(s)	177, 489, 537, 749, 932, 1014, 1113, 1772, 2251

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.