pycom06g20070
MYB Family

Myb-related protein 308-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001619
Physical Location & Seq
Forward (+)
23248255 .. 23250038
1784 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g20070.1

Sequence Viewer

Length: 600 bp
ATGAGGAAACCTTGCTGTGAGAAGAGAAAGACGAAAAAAGGAGCATGGTCGAAGCAAGAAGATGAGAAGCTCACTCAATATGTTGAAAAAAATGGTGAAGGAATCTGGAGTTCCCTTCCACTAGCTGCAGGCTTGCTTCGTTGTGGAAAGAGCTGTAGATTAAGATGGGTAAATTATCTAAGGCCAACCGTGAAACGTGGCAACTTTGGTGAAGATGAAGAGGACCTAATCATTAGGCTCCATGCACTTCTTGGAAACAGGTGGTCGTTAATTGCTGGAAGATTGCCGGGACGAACTGACAATGAAGTGAAGAATCACTGGAACACTCATCTAAGGAGAAAACTAATACAAATGGGAGTTGACCCCAATAACCATCGCATAGGACACACACACAATATTGGCCTTACAAAACCATCATTTGGCTCCAGAAAAGTAAATTATCCCTCTGAACCTGTTGATTCTCAAGGGGATAAAGACTCTGATCAAATTAAACCATTGTCAGATTCTACCAGTGGCCTGGAAAGCAACACAAGCTGCAGTGGTTTGCCTGACCTCAATCTTGATATCACGATTAGCCCTCCAAGTTCATTCGGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

200

Amino Acids

22.38

Weight (kDa)

9.33

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 13 - 60 1.1e-13 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 16 - 74 3.3e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 66 - 111 9.7e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 114 2.4e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 419
AfiI CCNNNNNNNGG 1 cut(s) 419
AgsI TTSAA 1 cut(s) 86
AjnI CCWGG 1 cut(s) 516
AluBI AGCT 4 cut(s) 70, 125, 153, 534
AluI AGCT 4 cut(s) 70, 125, 153, 534
AoxI GGCC 3 cut(s) 182, 400, 514
ApeKI GCWGC 2 cut(s) 125, 534
AspS9I GGNCC 1 cut(s) 223
AsuC2I CCSGG 1 cut(s) 288
AsuHPI GGTGA 2 cut(s) 107, 221
AvaII GGWCC 1 cut(s) 223
BbvI GCAGC 2 cut(s) 112, 521
BccI CCATC 3 cut(s) 159, 381, 421
BciT130I CCWGG 1 cut(s) 518
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 1 cut(s) 288
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 3 cut(s) 126, 154, 535
BfuI GTATCC 1 cut(s) 587
BisI GCNGC 2 cut(s) 126, 535
BlsI GCNGC 2 cut(s) 127, 536
Bme1390I CCNGG 2 cut(s) 288, 518
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 223
BmiI GGNNCC 2 cut(s) 239, 424
BmrFI CCNGG 2 cut(s) 288, 518
BpmI CTGGAG 2 cut(s) 127, 409
BpuEI CTTGAG 1 cut(s) 447
BpuMI CCSGG 1 cut(s) 288
Bsc4I CCNNNNNNNGG 1 cut(s) 419
Bse1I ACTGG 2 cut(s) 323, 510
BseBI CCWGG 1 cut(s) 518
BseLI CCNNNNNNNGG 1 cut(s) 419
BseNI ACTGG 2 cut(s) 323, 510
BseXI GCAGC 2 cut(s) 112, 521
BshFI GGCC 3 cut(s) 184, 402, 516
BsiSI CCGG 1 cut(s) 287
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 1 cut(s) 419
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 3 cut(s) 184, 402, 516
Bsp143I GATC 1 cut(s) 481
BspANI GGCC 3 cut(s) 184, 402, 516
BspLI GGNNCC 2 cut(s) 239, 424
BspMAI CTGCAG 2 cut(s) 130, 539
BsrI ACTGG 2 cut(s) 323, 510
BssMI GATC 1 cut(s) 481
Bst2UI CCWGG 1 cut(s) 518
Bst4CI ACNGT 1 cut(s) 190
Bst6I CTCTTC 2 cut(s) 17, 213
BstC8I GCNNGC 2 cut(s) 130, 134
BstDEI CTNAG 2 cut(s) 179, 332
BstKTI GATC 1 cut(s) 484
BstMBI GATC 1 cut(s) 481
BstMWI GCNNNNNNNGC 2 cut(s) 522, 531
BstNI CCWGG 1 cut(s) 518
BstSCI CCNGG 2 cut(s) 286, 516
BstSFI CTRYAG 3 cut(s) 126, 154, 535
BstV1I GCAGC 2 cut(s) 112, 521
BstXI CCANNNNNNTGG 1 cut(s) 517
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 3 cut(s) 184, 402, 516
BtgZI GCGATG 1 cut(s) 359
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 3 cut(s) 316, 517, 544
Cac8I GCNNGC 2 cut(s) 130, 134
Cfr13I GGNCC 1 cut(s) 223
CviAII CATG 2 cut(s) 45, 242
DdeI CTNAG 2 cut(s) 179, 332
DpnI GATC 1 cut(s) 483
DpnII GATC 1 cut(s) 481
Eam1104I CTCTTC 2 cut(s) 17, 213
EarI CTCTTC 2 cut(s) 17, 213
Eco32I GATATC 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 516
EcoRV GATATC 1 cut(s) 565
FaeI CATG 2 cut(s) 48, 245
FaiI YATR 4 cut(s) 46, 81, 243, 380
FaqI GGGAC 1 cut(s) 303
FatI CATG 2 cut(s) 44, 241
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 2 cut(s) 126, 535
Fsp4HI GCNGC 2 cut(s) 126, 535
FspBI CTAG 1 cut(s) 122
GluI GCNGC 2 cut(s) 126, 535
GsuI CTGGAG 2 cut(s) 127, 409
HaeIII GGCC 3 cut(s) 184, 402, 516
HapII CCGG 1 cut(s) 287
Hin1II CATG 2 cut(s) 48, 245
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HinfI GANTC 5 cut(s) 102, 313, 458, 476, 503
HpaII CCGG 1 cut(s) 287
HphI GGTGA 2 cut(s) 107, 221
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 4 cut(s) 448, 481, 502, 593
Hpy188III TCNNGA 4 cut(s) 106, 426, 560, 568
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 2 cut(s) 92, 125
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 196
HpyCH4V TGCA 3 cut(s) 128, 245, 537
HpyF10VI GCNNNNNNNGC 2 cut(s) 522, 531
HpyF3I CTNAG 2 cut(s) 179, 332
HpySE526I ACGT 1 cut(s) 196
Hsp92II CATG 2 cut(s) 48, 245
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 1 cut(s) 481
LmnI GCTCC 3 cut(s) 41, 243, 428
Lsp1109I GCAGC 2 cut(s) 112, 521
MaeI CTAG 1 cut(s) 122
MaeII ACGT 1 cut(s) 196
MalI GATC 1 cut(s) 483
MboI GATC 1 cut(s) 481
MboII GAAGA 6 cut(s) 34, 71, 224, 230, 291, 322
MluCI AATT 4 cut(s) 172, 270, 436, 486
MlyI GAGTC 1 cut(s) 470
MnlI CCTC 4 cut(s) 214, 454, 563, 588
MseI TTAA 3 cut(s) 161, 269, 489
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 2 cut(s) 288, 518
MvaI CCWGG 1 cut(s) 518
MwoI GCNNNNNNNGC 2 cut(s) 522, 531
NciI CCSGG 1 cut(s) 288
NdeII GATC 1 cut(s) 481
NlaIII CATG 2 cut(s) 48, 245
NlaIV GGNNCC 2 cut(s) 239, 424
PfeI GAWTC 4 cut(s) 102, 313, 458, 503
PflMI CCANNNNNTGG 1 cut(s) 419
PkrI GCNGC 2 cut(s) 127, 536
PleI GAGTC 1 cut(s) 470
PpsI GAGTC 1 cut(s) 470
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 1 cut(s) 516
PspGI CCWGG 1 cut(s) 516
PspN4I GGNNCC 2 cut(s) 239, 424
PspPI GGNCC 1 cut(s) 223
PspPPI RGGWCCY 1 cut(s) 223
PstI CTGCAG 2 cut(s) 130, 539
SaqAI TTAA 3 cut(s) 161, 269, 489
SatI GCNGC 2 cut(s) 126, 535
Sau3AI GATC 1 cut(s) 481
Sau96I GGNCC 1 cut(s) 223
SchI GAGTC 1 cut(s) 470
ScrFI CCNGG 2 cut(s) 288, 518
SfcI CTRYAG 3 cut(s) 126, 154, 535
SinI GGWCC 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 462
SmoI CTYRAG 1 cut(s) 462
Sse9I AATT 4 cut(s) 172, 270, 436, 486
SspI AATATT 1 cut(s) 397
SspMI CTAG 1 cut(s) 122
StyD4I CCNGG 2 cut(s) 286, 516
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 1 cut(s) 199
TaqI TCGA 1 cut(s) 50
TasI AATT 4 cut(s) 172, 270, 436, 486
TfiI GAWTC 4 cut(s) 102, 313, 458, 503
Tru1I TTAA 3 cut(s) 161, 269, 489
Tru9I TTAA 3 cut(s) 161, 269, 489
TscAI CASTG 3 cut(s) 323, 517, 544
TseI GCWGC 2 cut(s) 125, 534
TspDTI ATGAA 3 cut(s) 231, 318, 576
TspRI CASTG 3 cut(s) 323, 517, 544
Van91I CCANNNNNTGG 1 cut(s) 419
VpaK11BI GGWCC 1 cut(s) 223
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XspI CTAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.