MD13G1026400.v1.1
MYB Family

SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001619
Physical Location & Seq
Reverse (-)
1905470 .. 1907202
1733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1026400.v1.1.491

Sequence Viewer

Length: 609 bp
ATGAGGAAACCCTGCTGCGATAAGCAAGACACAAACAAAGGAGCTTGGTCCAAGCAAGAAGACCTCAAGCTCATCGATTACATTCGTAAACATGGCGAGGGTTGTTGGCGTACCCTTCCTCAGGCTGCAGGCCTACTTCGTTGTGGTAAAAGTTGTAGGCTGAGATGGATAAACTATCTACGACCAGACCTCAAAAGAGGCAACTTTGCAGAAGATGAAGAAGATCTCATCGTCAAGCTTCATGCGCTCCTAGGCAACAGGTGGTCATTGATTGCTGGGAGATTGCCAGGACGTACAGACAATGAAGTGAAAAACTATTGGAACTCTCATTTGAGAAGAAAGCTTATGAGCATGGGTATAGATCCAAATAATCATCGACCGAACAACGTTAATCTTTCTCGTCTTCATCATCAAAATTCACAAACTGTCAGTAGTACTGCAACATTGTCTGCAGCTTTAAAAAACCCTAGCAATTATCAGCAGCAAGCAAGATCCAGTGGTAATAATAGTTGCGACCAAAAATCGGACGGGACAAGTTGCTTAGAGGATGATTCTTGTGGCCGGCTGCCTGATTTAAACCTAGACCTCACAATAACTGCTCCTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

203

Amino Acids

22.87

Weight (kDa)

9.17

Isoelectric Point (pI)

51.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 13 - 60 2.6e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 16 - 74 4.4e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 66 - 111 5.8e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 115 2.5e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 387
AclWI GGATC 2 cut(s) 356, 486
AcoI YGGCCR 1 cut(s) 559
AcsI RAATTY 1 cut(s) 415
AfaI GTAC 3 cut(s) 112, 295, 436
AfiI CCNNNNNNNGG 2 cut(s) 121, 523
AjnI CCWGG 1 cut(s) 286
AluBI AGCT 5 cut(s) 44, 70, 238, 343, 455
AluI AGCT 5 cut(s) 44, 70, 238, 343, 455
AlwI GGATC 2 cut(s) 356, 486
AoxI GGCC 2 cut(s) 130, 559
ApeKI GCWGC 5 cut(s) 15, 125, 452, 481, 565
ApoI RAATTY 1 cut(s) 415
AspA2I CCTAGG 1 cut(s) 250
AspLEI GCGC 1 cut(s) 247
AspS9I GGNCC 1 cut(s) 48
AvaII GGWCC 1 cut(s) 48
AvrII CCTAGG 1 cut(s) 250
AxyI CCTNAGG 1 cut(s) 120
BbsI GAAGAC 2 cut(s) 66, 395
BbvI GCAGC 5 cut(s) 2, 112, 464, 493, 552
BccI CCATC 1 cut(s) 159
BciT130I CCWGG 1 cut(s) 288
BfaI CTAG 3 cut(s) 251, 468, 581
BfmI CTRYAG 2 cut(s) 126, 450
BglII AGATCT 1 cut(s) 223
BisI GCNGC 5 cut(s) 16, 126, 453, 482, 566
BlnI CCTAGG 1 cut(s) 250
BlsI GCNGC 5 cut(s) 17, 127, 454, 483, 567
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 1 cut(s) 288
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 48
BmrFI CCNGG 1 cut(s) 288
BpiI GAAGAC 2 cut(s) 66, 395
BpuEI CTTGAG 1 cut(s) 50
Bsa29I ATCGAT 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 250
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 523
Bse118I RCCGGY 1 cut(s) 561
Bse1I ACTGG 1 cut(s) 495
Bse21I CCTNAGG 1 cut(s) 120
BseBI CCWGG 1 cut(s) 288
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 250
BseGI GGATG 1 cut(s) 553
BseLI CCNNNNNNNGG 2 cut(s) 121, 523
BseMII CTCAG 2 cut(s) 134, 152
BseNI ACTGG 1 cut(s) 495
BseXI GCAGC 5 cut(s) 2, 112, 464, 493, 552
BseYI CCCAGC 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 380
BshFI GGCC 2 cut(s) 132, 561
BshVI ATCGAT 1 cut(s) 75
BsiEI CGRYCG 1 cut(s) 380
BsiSI CCGG 1 cut(s) 562
BslFI GGGAC 1 cut(s) 544
BslI CCNNNNNNNGG 2 cut(s) 121, 523
BsmFI GGGAC 1 cut(s) 544
BsnI GGCC 2 cut(s) 132, 561
Bsp143I GATC 3 cut(s) 223, 361, 491
BspANI GGCC 2 cut(s) 132, 561
BspCNI CTCAG 2 cut(s) 133, 153
BspDI ATCGAT 1 cut(s) 75
BspMAI CTGCAG 2 cut(s) 130, 454
BspPI GGATC 2 cut(s) 356, 486
BsrFI RCCGGY 1 cut(s) 561
BsrI ACTGG 1 cut(s) 495
BssAI RCCGGY 1 cut(s) 561
BssECI CCNNGG 1 cut(s) 250
BssMI GATC 3 cut(s) 223, 361, 491
BssT1I CCWWGG 1 cut(s) 250
Bst2UI CCWGG 1 cut(s) 288
Bst4CI ACNGT 1 cut(s) 427
BstC8I GCNNGC 3 cut(s) 130, 486, 563
BstDEI CTNAG 3 cut(s) 120, 161, 541
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 1 cut(s) 553
BstHHI GCGC 1 cut(s) 247
BstKTI GATC 3 cut(s) 226, 364, 494
BstMBI GATC 3 cut(s) 223, 361, 491
BstMCI CGRYCG 1 cut(s) 380
BstMWI GCNNNNNNNGC 1 cut(s) 244
BstNI CCWGG 1 cut(s) 288
BstSCI CCNGG 1 cut(s) 286
BstSFI CTRYAG 2 cut(s) 126, 450
BstV1I GCAGC 5 cut(s) 2, 112, 464, 493, 552
BstV2I GAAGAC 2 cut(s) 66, 395
BstX2I RGATCY 3 cut(s) 223, 361, 491
BstYI RGATCY 3 cut(s) 223, 361, 491
Bsu15I ATCGAT 1 cut(s) 75
Bsu36I CCTNAGG 1 cut(s) 120
BsuRI GGCC 2 cut(s) 132, 561
BsuTUI ATCGAT 1 cut(s) 75
BtsCI GGATG 1 cut(s) 553
BtsIMutI CAGTG 1 cut(s) 502
Cac8I GCNNGC 3 cut(s) 130, 486, 563
CfoI GCGC 1 cut(s) 247
Cfr10I RCCGGY 1 cut(s) 561
Cfr13I GGNCC 1 cut(s) 48
ClaI ATCGAT 1 cut(s) 75
Csp6I GTAC 3 cut(s) 111, 294, 435
CviAII CATG 3 cut(s) 92, 242, 352
CviQI GTAC 3 cut(s) 111, 294, 435
DdeI CTNAG 3 cut(s) 120, 161, 541
DpnI GATC 3 cut(s) 225, 363, 493
DpnII GATC 3 cut(s) 223, 361, 491
DraI TTTAAA 2 cut(s) 459, 576
EaeI YGGCCR 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 250
Eco147I AGGCCT 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 48
Eco81I CCTNAGG 1 cut(s) 120
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 286
EcoT14I CCWWGG 1 cut(s) 250
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 3 cut(s) 95, 245, 355
FaiI YATR 5 cut(s) 93, 243, 347, 353, 359
FaqI GGGAC 1 cut(s) 544
FatI CATG 3 cut(s) 91, 241, 351
Fnu4HI GCNGC 5 cut(s) 16, 126, 453, 482, 566
FokI GGATG 1 cut(s) 560
Fsp4HI GCNGC 5 cut(s) 16, 126, 453, 482, 566
FspBI CTAG 3 cut(s) 251, 468, 581
GlaI GCGC 1 cut(s) 246
GluI GCNGC 5 cut(s) 16, 126, 453, 482, 566
GsaI CCCAGC 1 cut(s) 279
HaeIII GGCC 2 cut(s) 132, 561
HapII CCGG 1 cut(s) 562
HhaI GCGC 1 cut(s) 247
Hin1II CATG 3 cut(s) 95, 245, 355
Hin6I GCGC 1 cut(s) 245
HinP1I GCGC 1 cut(s) 245
HindIII AAGCTT 2 cut(s) 236, 341
HinfI GANTC 1 cut(s) 551
HpaII CCGG 1 cut(s) 562
Hpy166II GTNNAC 1 cut(s) 89
Hpy188I TCNGA 1 cut(s) 526
Hpy8I GTNNAC 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 2 cut(s) 292, 387
HpyCH4V TGCA 4 cut(s) 128, 209, 440, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 244
HpyF3I CTNAG 3 cut(s) 120, 161, 541
HpySE526I ACGT 2 cut(s) 292, 387
Hsp92II CATG 3 cut(s) 95, 245, 355
HspAI GCGC 1 cut(s) 245
KroI GCCGGC 1 cut(s) 561
KroNI GCCGGC 1 cut(s) 563
Kzo9I GATC 3 cut(s) 223, 361, 491
LmnI GCTCC 3 cut(s) 41, 252, 604
Lsp1109I GCAGC 5 cut(s) 2, 112, 464, 493, 552
MaeI CTAG 3 cut(s) 251, 468, 581
MaeII ACGT 2 cut(s) 292, 387
MalI GATC 3 cut(s) 225, 363, 493
MboI GATC 3 cut(s) 223, 361, 491
MboII GAAGA 6 cut(s) 71, 224, 230, 233, 348, 395
MflI RGATCY 3 cut(s) 223, 361, 491
MluCI AATT 2 cut(s) 415, 472
MnlI CCTC 7 cut(s) 74, 91, 129, 191, 200, 538, 596
MroNI GCCGGC 1 cut(s) 561
MseI TTAA 3 cut(s) 390, 458, 575
MspI CCGG 1 cut(s) 562
MspR9I CCNGG 1 cut(s) 288
MvaI CCWGG 1 cut(s) 288
MwoI GCNNNNNNNGC 1 cut(s) 244
NaeI GCCGGC 1 cut(s) 563
NdeII GATC 3 cut(s) 223, 361, 491
NgoMIV GCCGGC 1 cut(s) 561
NlaIII CATG 3 cut(s) 95, 245, 355
PceI AGGCCT 1 cut(s) 132
PdiI GCCGGC 1 cut(s) 563
PfeI GAWTC 1 cut(s) 551
PkrI GCNGC 5 cut(s) 17, 127, 454, 483, 567
Psp1406I AACGTT 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 286
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 1 cut(s) 286
PspPI GGNCC 1 cut(s) 48
PstI CTGCAG 2 cut(s) 130, 454
PsuI RGATCY 3 cut(s) 223, 361, 491
RsaI GTAC 3 cut(s) 112, 295, 436
RsaNI GTAC 3 cut(s) 111, 294, 435
SaqAI TTAA 3 cut(s) 390, 458, 575
SatI GCNGC 5 cut(s) 16, 126, 453, 482, 566
Sau3AI GATC 3 cut(s) 223, 361, 491
Sau96I GGNCC 1 cut(s) 48
ScaI AGTACT 1 cut(s) 436
ScrFI CCNGG 1 cut(s) 288
SfcI CTRYAG 2 cut(s) 126, 450
SinI GGWCC 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 2 cut(s) 415, 472
SseBI AGGCCT 1 cut(s) 132
SspMI CTAG 3 cut(s) 251, 468, 581
StuI AGGCCT 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 286
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 2 cut(s) 295, 390
TaqI TCGA 2 cut(s) 75, 376
TaqII GACCGA 1 cut(s) 394
TasI AATT 2 cut(s) 415, 472
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 3 cut(s) 390, 458, 575
Tru9I TTAA 3 cut(s) 390, 458, 575
TscAI CASTG 1 cut(s) 502
TseI GCWGC 5 cut(s) 15, 125, 452, 481, 565
TspDTI ATGAA 4 cut(s) 230, 231, 318, 395
TspRI CASTG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 48
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 1 cut(s) 415
XmaJI CCTAGG 1 cut(s) 250
XspI CTAG 3 cut(s) 251, 468, 581
ZrmI AGTACT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.